Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,597,097 G→C 7.4% V555V (GTC→GTG)  dctB ← C4‑dicarboxylate transport sensor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,597,0970GC 7.4% 168.3 / 2.7 53V555V (GTC→GTG) dctBC4‑dicarboxylate transport sensor protein
Reads supporting (aligned to +/- strand):  ref base G (22/27);  new base C (2/2);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCGACAGCAGTGCGGATACTAGCTTCATCATCGACGACGATCACCGAATTCAACATACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTCGGCAATGCCGCCACCCGAGTCGAGCACACTCAGGCGCCATAGGTCCTCGTC  >  NC_002947/1596954‑1597243
                                                                                                                                               |                                                                                                                                                  
cTCGACAGCAGTGCGGATACTAGCTTCATCATCGACGACGATCACCGAATTCAACATACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGa                                                                                                                                                <  8:151746/148‑1 (MQ=255)
   gACAGCAGTGCGGATACTAGCTTCATCATCGACGACGATCACCGAATTCAACATACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGcc                                                                                                                                            >  6:138936/1‑149 (MQ=255)
      agcagTGCGGATACTAGCTTCATCATCGACGACGATCACCGAATTCAACATACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTa                                                                                                                                         <  6:152230/149‑1 (MQ=255)
             cGGATACTAGCTTCATCATCGACGACGATCACCGAATTCAACATACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAAt                                                                                                                                   <  4:2270/148‑1 (MQ=255)
             cGGATACTAGCTTCATCATCGACGACGATCACCGAATTCAACATACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATg                                                                                                                                  <  3:28121/149‑1 (MQ=255)
                  aCTAGCTTCATCATCGACGACGATCACCGAATTCAACATACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAg                                                                                                                             >  8:254886/1‑149 (MQ=255)
                             catcGACGACGATCACCGAATTCAACATACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAg                                                                                                                             >  1:298815/1‑138 (MQ=255)
                             catcGACGACGATCACCGAATTCAACATACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAg                                                                                                                             <  2:298815/138‑1 (MQ=255)
                              atcGACGACGATCACCGAATTCAACATACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCagccctag                                                                                                                 >  4:77000/1‑149 (MQ=255)
                                 gacgacgaTCACCGAATTCAACATACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCagccctagccc                                                                                                              >  3:92720/1‑149 (MQ=255)
                                     acgcTCACCGAATTCAACATACAGGCTCCAGGGCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTcc                                                                                                          <  6:14365/149‑1 (MQ=255)
                                            ccGAATTCAACATACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCg                                                                                                   >  7:44/1‑149 (MQ=255)
                                               aaTTCAACATACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGtt                                                                                                <  8:243160/149‑1 (MQ=255)
                                                    aaCATACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTtggtg                                                                                           >  5:119239/1‑149 (MQ=255)
                                                    aaCATACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTtggtg                                                                                           <  4:278285/149‑1 (MQ=255)
                                                    aaCATACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTtggtg                                                                                           <  7:71774/149‑1 (MQ=255)
                                                    aaCATACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTtggtg                                                                                           >  2:175899/1‑149 (MQ=255)
                                                    aaCATACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTACCCCATCCCCCACCGGTTtggtg                                                                                           >  8:144088/1‑149 (MQ=255)
                                                     aCATACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTtggtgg                                                                                          <  2:199621/149‑1 (MQ=255)
                                                        tACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGgtggtg                                                                                        >  7:273752/1‑148 (MQ=255)
                                                        tACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTga                                                                                       <  8:44/149‑1 (MQ=255)
                                                             gCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAAc                                                                                  <  6:119239/149‑1 (MQ=255)
                                                                 cAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGt                                                                              <  3:77000/149‑1 (MQ=255)
                                                                  aGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTc                                                                             <  2:207475/149‑1 (MQ=255)
                                                                     tCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGaa                                                                          <  5:119991/149‑1 (MQ=255)
                                                                            gCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTg                                                                   <  5:138936/149‑1 (MQ=255)
                                                                                      gCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCg                                                                            >  6:286936/1‑130 (MQ=255)
                                                                                      gCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCg                                                                            <  5:286936/130‑1 (MQ=255)
                                                                                          aGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCCAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTc                                                     >  2:19459/1‑149 (MQ=255)
                                                                                            gcgctcgcCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCGGTAGTAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTCgg                                                   <  7:144088/149‑1 (MQ=255)
                                                                                            gcgcgcgcCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTCgg                                                   >  6:199023/1‑149 (MQ=255)
                                                                                                gcgcCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTCGGCAAt                                               >  6:67347/1‑149 (MQ=255)
                                                                                                 cgccgcCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTCGGCAATg                                              <  7:254886/149‑1 (MQ=255)
                                                                                                   ccgccAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGCACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTCGGCAATgcc                                            <  5:199023/149‑1 (MQ=255)
                                                                                                              gTTTTCGGGCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTCGGCAATGCCGCCACCCGAGt                                 <  8:227195/149‑1 (MQ=255)
                                                                                                               ttttCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTCGGCAATGCCGCCACCCGAGTc                                >  3:305529/1‑149 (MQ=255)
                                                                                                               ttttCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTCGGCAATGCCGCCACCCGAGTc                                >  7:133038/1‑149 (MQ=255)
                                                                                                               ttttCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGACGAAGACCTTGGCCAGGTCGGCTTCGGCAATGCCGCCACCCGAGTc                                >  7:240038/1‑149 (MQ=255)
                                                                                                                tttCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTTGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCg                                                          >  6:69137/1‑122 (MQ=255)
                                                                                                                tttCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCg                                                          <  5:69137/122‑1 (MQ=255)
                                                                                                                  tCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGTCTTCGGCAATGCCGCCACCCGAGTCGa                              <  1:175899/148‑1 (MQ=255)
                                                                                                                  tCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTCGGCAATGCCGCCACCCGAGTCGa                              <  3:307052/148‑1 (MQ=255)
                                                                                                                    ggCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTCGGCAATGCCGCCACCCGAGTCGAGc                            <  4:92720/148‑1 (MQ=255)
                                                                                                                       ccGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCTGGCCCAGCCCTAGCCCTTCCCCCCCCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTCGGCAATGCCGCCACCCGAGTCGAGCacac                        >  1:239460/1‑149 (MQ=255)
                                                                                                                       ccGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCTCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTc                                                     <  3:317951/120‑1 (MQ=255)
                                                                                                                       ccGCAGCTGGCCGCCCGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCACTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTc                                                     >  4:317951/1‑120 (MQ=255)
                                                                                                                         gCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCt                                                                     <  6:134602/102‑1 (MQ=255)
                                                                                                                         gCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCt                                                                     >  5:134602/1‑102 (MQ=255)
                                                                                                                                  ctacgGCATCGTCCACGAGGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTCGGCAATGCCGCCACCCGAGTCGAGCACACTCAGGCGCCa              >  7:139078/4‑148 (MQ=255)
                                                                                                                                     cgGCATCGTCCACGAGGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTCGGCAATGCCGCCACCCGAGTCGAGCACACTCAGGCGCCAt             <  7:254801/146‑1 (MQ=255)
                                                                                                                                     cgGCATCGTCCACGAGGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTCGGCAATGCCGCCACCCGAGTCGAGCACACTCAGGCGCCAt             >  8:254801/1‑146 (MQ=255)
                                                                                                                                         cTCGTGGACGAAGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTCGGCAATGCCGCCACCCGAGTCGAGCACACTCAGGCGCCATAGGTCCt      >  2:73394/1‑149 (MQ=255)
                                                                                                                                             tGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTCGGCAATGCCGCCACCCGAGTCGAGCACACTCAGGCGCCATAGGTCCtcgtc  <  8:273752/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
CTCGACAGCAGTGCGGATACTAGCTTCATCATCGACGACGATCACCGAATTCAACATACAGGCTCCAGGTCACGGGGCAGGGTAAGGCTCAGGCGCGCGCCGCCAGGCAGGTTTTCGGCCCGCAGCTGGCCGCCGGCCTCGTGGACGATGCCGTAGGAAATGGCCAGGCCCAGCCCTAGCCCTTCCCCCACCGGTTTGGTGGTGAAGAACGGGTCGAAGACCTTGGCCAGGTCGGCTTCGGCAATGCCGCCACCCGAGTCGAGCACACTCAGGCGCCATAGGTCCTCGTC  >  NC_002947/1596954‑1597243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑