Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,611,208 C→G 8.7% E290D (GAG→GAC)  PP_1412 ← 3‑hydroxyisobutyryl‑CoA hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,611,2080CG 8.7% 139.2 / 4.4 46E290D (GAG→GAC) PP_14123‑hydroxyisobutyryl‑CoA hydrolase
Reads supporting (aligned to +/- strand):  ref base C (21/21);  new base G (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.02e-01

CCAGCGCGGCTGGTTGTCTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTGATCGATCTCAT  >  NC_002947/1611067‑1611356
                                                                                                                                             |                                                                                                                                                    
ccAGCGCGGCTGGTTGTCTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCccaga                                                                                                                                               <  7:27888/149‑1 (MQ=255)
     gcgGCTGGTTGTCTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCccagaccaga                                                                                                                                          <  8:180759/149‑1 (MQ=255)
     gcgGCTGGTTGTCTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCccagaccaga                                                                                                                                          <  2:232972/149‑1 (MQ=255)
            gTTGTCTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGCATCTGGTCTGGGAGCAGATCCGcc                                                                                                                                    <  8:151141/148‑1 (MQ=255)
              tGTCTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTc                                                                                                                                 >  6:91032/1‑149 (MQ=255)
                 cTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGc                                                                                                                              >  1:151336/1‑149 (MQ=255)
                         agcagGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAAcc                                                                                                                      >  7:176085/1‑149 (MQ=255)
                            agGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTGCCAGACCAGATGCGCCGTCTGCGGGCAACCCTc                                                                                                                   <  1:285115/149‑1 (MQ=255)
                              gCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCAt                                                                                                                 <  7:172385/149‑1 (MQ=255)
                                 aGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATg                                                                                                                >  2:198769/1‑147 (MQ=255)
                                      gcACGCCTTCACTGAATTCAGGCTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCGTCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCCGCGGGCACCCCGCATGCAGCCgg                                                                                                         >  7:50342/1‑149 (MQ=255)
                                                  tGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCt                                                                                             >  3:89850/1‑149 (MQ=255)
                                                    aaTTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGc                                                                                           >  4:81176/1‑149 (MQ=255)
                                                                    aaCAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCa                                                                           >  5:77053/1‑149 (MQ=255)
                                                                    aaCAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCa                                                                           >  8:27271/1‑149 (MQ=255)
                                                                           cAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATgctgct                                                                    >  6:238981/1‑149 (MQ=255)
                                                                            acgcccATGCTGTATTCCCTCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCtt                                                                   <  8:50342/144‑1 (MQ=255)
                                                                              gCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATTCTGCTTGa                                                                 <  4:89850/149‑1 (MQ=255)
                                                                              gCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGa                                                                 <  6:77053/149‑1 (MQ=255)
                                                                                          ttCCATCTGGCACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAgc                                                      <  6:269325/148‑1 (MQ=255)
                                                                                             cATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAgcgcgc                                                  >  8:245882/1‑149 (MQ=255)
                                                                                             cATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAgcgcgc                                                  <  5:91032/149‑1 (MQ=255)
                                                                                                   gAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCAtgc                                                                        <  1:286382/121‑1 (MQ=255)
                                                                                                   gAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCAtgc                                                                        >  2:286382/1‑121 (MQ=255)
                                                                                                   gAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAg                                             <  3:81176/148‑1 (MQ=255)
                                                                                                   gAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGGGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTTCTTGAGCCTTTCCAGCGCGCGCCAgg                                            <  8:176085/149‑1 (MQ=255)
                                                                                                    aaCACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCGGGc                                           <  2:151336/149‑1 (MQ=255)
                                                                                                        ccTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGaa                                       <  6:247708/149‑1 (MQ=255)
                                                                                                         cTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAgg                                            >  7:208773/1‑143 (MQ=255)
                                                                                                         cTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAgg                                            <  8:208773/143‑1 (MQ=255)
                                                                                                         cTGCGCCAACGACAGGTACCGCGCCCAGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAg                                      >  7:85069/1‑149 (MQ=255)
                                                                                                                aaCGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATc                               >  3:301001/1‑149 (MQ=255)
                                                                                                                aaCGACAGGTACCGCACCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATc                               >  3:321938/1‑149 (MQ=255)
                                                                                                                   gACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGc                            >  7:295675/1‑149 (MQ=255)
                                                                                                                   gACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGc                            <  4:301001/149‑1 (MQ=255)
                                                                                                                      aGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCAc                         <  7:27271/149‑1 (MQ=255)
                                                                                                                        gTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGt                       <  1:198769/149‑1 (MQ=255)
                                                                                                                        gTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGt                       >  4:166851/1‑149 (MQ=255)
                                                                                                                           ccGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGa                    <  3:166851/149‑1 (MQ=255)
                                                                                                                           ccGCGCCCGGCGGATCTGCTCCCAGACCAGAAGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTc                      >  5:210924/1‑147 (MQ=255)
                                                                                                                            cgcgCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAg                   >  6:51102/1‑149 (MQ=255)
                                                                                                                              ctgacggcgcATCTGGTCTGGGAGCAGATCCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGt                  >  5:263654/5‑148 (MQ=38)
                                                                                                                                cccGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAAc               >  5:243058/1‑149 (MQ=255)
                                                                                                                                   ggcgcATCTGGTCTGGGAGCAGATCCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGa                                                                 <  1:141120/96‑1 (MQ=37)
                                                                                                                                   ggcgcATCTGGTCTGGGAGCAGATCCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGa                                                                 >  2:141120/1‑96 (MQ=37)
                                                                                                                                      ggATCTGCTCCCAGACCAGATACGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTgatcg         <  6:273080/149‑1 (MQ=255)
                                                                                                                                             cTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTGATCGATCTCAt  >  2:172716/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
CCAGCGCGGCTGGTTGTCTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTGATCGATCTCAT  >  NC_002947/1611067‑1611356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑