Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,611,213 A→G 8.3% W289R (TGG→CGG) ‡ PP_1412 ← 3‑hydroxyisobutyryl‑CoA hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,611,2130AG 8.3% 133.6 / 3.0 48W289R (TGG→CGG) ‡PP_14123‑hydroxyisobutyryl‑CoA hydrolase
Reads supporting (aligned to +/- strand):  ref base A (25/19);  new base G (2/2);  total (27/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01

CCAGCGCGGCTGGTTGTCTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTGATCGATCTCATGCCG  >  NC_002947/1611067‑1611360
                                                                                                                                                  |                                                                                                                                                   
ccAGCGCGGCTGGTTGTCTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCccaga                                                                                                                                                   <  7:27888/149‑1 (MQ=255)
     gcgGCTGGTTGTCTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCccagaccaga                                                                                                                                              <  8:180759/149‑1 (MQ=255)
     gcgGCTGGTTGTCTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCccagaccaga                                                                                                                                              <  2:232972/149‑1 (MQ=255)
            gTTGTCTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGCATCTGGTCTGGGAGCAGATCCGcc                                                                                                                                        <  8:151141/148‑1 (MQ=255)
              tGTCTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTc                                                                                                                                     >  6:91032/1‑149 (MQ=255)
                 cTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGc                                                                                                                                  >  1:151336/1‑149 (MQ=255)
                         agcagGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAAcc                                                                                                                          >  7:176085/1‑149 (MQ=255)
                            agGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTGCCAGACCAGATGCGCCGTCTGCGGGCAACCCTc                                                                                                                       <  1:285115/149‑1 (MQ=255)
                              gCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCAt                                                                                                                     <  7:172385/149‑1 (MQ=255)
                                 aGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATg                                                                                                                    >  2:198769/1‑147 (MQ=255)
                                      gcACGCCTTCACTGAATTCAGGCTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCGTCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCCGCGGGCACCCCGCATGCAGCCgg                                                                                                             >  7:50342/1‑149 (MQ=255)
                                                  tGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCt                                                                                                 >  3:89850/1‑149 (MQ=255)
                                                    aaTTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGc                                                                                               >  4:81176/1‑149 (MQ=255)
                                                                    aaCAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCa                                                                               >  5:77053/1‑149 (MQ=255)
                                                                    aaCAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCa                                                                               >  8:27271/1‑149 (MQ=255)
                                                                           cAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATgctgct                                                                        >  6:238981/1‑149 (MQ=255)
                                                                            acgcccATGCTGTATTCCCTCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCtt                                                                       <  8:50342/144‑1 (MQ=255)
                                                                              gCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATTCTGCTTGa                                                                     <  4:89850/149‑1 (MQ=255)
                                                                              gCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGa                                                                     <  6:77053/149‑1 (MQ=255)
                                                                                          ttCCATCTGGCACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAgc                                                          <  6:269325/148‑1 (MQ=255)
                                                                                             cATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAgcgcgc                                                      >  8:245882/1‑149 (MQ=255)
                                                                                             cATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAgcgcgc                                                      <  5:91032/149‑1 (MQ=255)
                                                                                                   gAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCAtgc                                                                            <  1:286382/121‑1 (MQ=255)
                                                                                                   gAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCAtgc                                                                            >  2:286382/1‑121 (MQ=255)
                                                                                                   gAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAg                                                 <  3:81176/148‑1 (MQ=255)
                                                                                                   gAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGGGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTTCTTGAGCCTTTCCAGCGCGCGCCAgg                                                <  8:176085/149‑1 (MQ=255)
                                                                                                    aaCACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCGGGc                                               <  2:151336/149‑1 (MQ=255)
                                                                                                        ccTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGaa                                           <  6:247708/149‑1 (MQ=255)
                                                                                                         cTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAgg                                                <  8:208773/143‑1 (MQ=255)
                                                                                                         cTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAgg                                                >  7:208773/1‑143 (MQ=255)
                                                                                                         cTGCGCCAACGACAGGTACCGCGCCCAGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAg                                          >  7:85069/1‑149 (MQ=255)
                                                                                                                aaCGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATc                                   >  3:301001/1‑149 (MQ=255)
                                                                                                                aaCGACAGGTACCGCACCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATc                                   >  3:321938/1‑149 (MQ=255)
                                                                                                                   gACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGc                                >  7:295675/1‑149 (MQ=255)
                                                                                                                   gACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGc                                <  4:301001/149‑1 (MQ=255)
                                                                                                                      aGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCAc                             <  7:27271/149‑1 (MQ=255)
                                                                                                                        gTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGt                           >  4:166851/1‑149 (MQ=255)
                                                                                                                        gTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGt                           <  1:198769/149‑1 (MQ=255)
                                                                                                                           ccGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGa                        <  3:166851/149‑1 (MQ=255)
                                                                                                                           ccGCGCCCGGCGGATCTGCTCCCAGACCAGAAGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTc                          >  5:210924/1‑147 (MQ=255)
                                                                                                                            cgcgCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAg                       >  6:51102/1‑149 (MQ=255)
                                                                                                                              ctgacggcgcATCTGGTCTGGGAGCAGATCCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGt                      >  5:263654/5‑148 (MQ=38)
                                                                                                                                cccGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAAc                   >  5:243058/1‑149 (MQ=255)
                                                                                                                                   ggcgcATCTGGTCTGGGAGCAGATCCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGa                                                                     >  2:141120/1‑96 (MQ=37)
                                                                                                                                   ggcgcATCTGGTCTGGGAGCAGATCCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGa                                                                     <  1:141120/96‑1 (MQ=37)
                                                                                                                                      ggATCTGCTCCCAGACCAGATACGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTgatcg             <  6:273080/149‑1 (MQ=255)
                                                                                                                                             cTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTGATCGATCTCAt      >  2:172716/1‑149 (MQ=255)
                                                                                                                                              tCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAAc                   <  8:19149/135‑1 (MQ=255)
                                                                                                                                              tCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTGATCGATCTCATg     >  3:84173/1‑149 (MQ=255)
                                                                                                                                              tCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCAACCGCCACGTCGAGTAAc                   >  7:19149/1‑135 (MQ=255)
                                                                                                                                               cccAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTGATCGATCTCATGc    >  3:291769/1‑149 (MQ=255)
                                                                                                                                                ccagaccagaTGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTGATCGATCTCATGcc   >  7:95021/1‑149 (MQ=255)
                                                                                                                                                 cagaccagaTGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTGATCGATCTCATGCcg  >  2:105901/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CCAGCGCGGCTGGTTGTCTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTGATCGATCTCATGCCG  >  NC_002947/1611067‑1611360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑