Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,611,216 C→G 8.3% V288L (GTC→CTC)  PP_1412 ← 3‑hydroxyisobutyryl‑CoA hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,611,2160CG 8.3% 136.6 / 4.3 48V288L (GTC→CTC) PP_14123‑hydroxyisobutyryl‑CoA hydrolase
Reads supporting (aligned to +/- strand):  ref base C (25/19);  new base G (2/2);  total (27/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-01

GCGGCTGGTTGTCTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTGATCGATCTCATGCCGTCG  >  NC_002947/1611072‑1611363
                                                                                                                                                |                                                                                                                                                   
gcgGCTGGTTGTCTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCccagaccaga                                                                                                                                                 <  8:180759/149‑1 (MQ=255)
gcgGCTGGTTGTCTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCccagaccaga                                                                                                                                                 <  2:232972/149‑1 (MQ=255)
       gTTGTCTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGCATCTGGTCTGGGAGCAGATCCGcc                                                                                                                                           <  8:151141/148‑1 (MQ=255)
         tGTCTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTc                                                                                                                                        >  6:91032/1‑149 (MQ=255)
            cTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGc                                                                                                                                     >  1:151336/1‑149 (MQ=255)
                    agcagGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAAcc                                                                                                                             >  7:176085/1‑149 (MQ=255)
                       agGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTGCCAGACCAGATGCGCCGTCTGCGGGCAACCCTc                                                                                                                          <  1:285115/149‑1 (MQ=255)
                         gCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCAt                                                                                                                        <  7:172385/149‑1 (MQ=255)
                            aGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATg                                                                                                                       >  2:198769/1‑147 (MQ=255)
                                 gcACGCCTTCACTGAATTCAGGCTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCGTCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCCGCGGGCACCCCGCATGCAGCCgg                                                                                                                >  7:50342/1‑149 (MQ=255)
                                             tGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCt                                                                                                    >  3:89850/1‑149 (MQ=255)
                                               aaTTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGc                                                                                                  >  4:81176/1‑149 (MQ=255)
                                                               aaCAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCa                                                                                  >  5:77053/1‑149 (MQ=255)
                                                               aaCAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCa                                                                                  >  8:27271/1‑149 (MQ=255)
                                                                      cAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATgctgct                                                                           >  6:238981/1‑149 (MQ=255)
                                                                       acgcccATGCTGTATTCCCTCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCtt                                                                          <  8:50342/144‑1 (MQ=255)
                                                                         gCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATTCTGCTTGa                                                                        <  4:89850/149‑1 (MQ=255)
                                                                         gCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGa                                                                        <  6:77053/149‑1 (MQ=255)
                                                                                     ttCCATCTGGCACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAgc                                                             <  6:269325/148‑1 (MQ=255)
                                                                                        cATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAgcgcgc                                                         >  8:245882/1‑149 (MQ=255)
                                                                                        cATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAgcgcgc                                                         <  5:91032/149‑1 (MQ=255)
                                                                                              gAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCAtgc                                                                               >  2:286382/1‑121 (MQ=255)
                                                                                              gAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCAtgc                                                                               <  1:286382/121‑1 (MQ=255)
                                                                                              gAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAg                                                    <  3:81176/148‑1 (MQ=255)
                                                                                              gAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGGGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTTCTTGAGCCTTTCCAGCGCGCGCCAgg                                                   <  8:176085/149‑1 (MQ=255)
                                                                                               aaCACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCGGGc                                                  <  2:151336/149‑1 (MQ=255)
                                                                                                   ccTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGaa                                              <  6:247708/149‑1 (MQ=255)
                                                                                                    cTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAgg                                                   <  8:208773/143‑1 (MQ=255)
                                                                                                    cTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAgg                                                   >  7:208773/1‑143 (MQ=255)
                                                                                                    cTGCGCCAACGACAGGTACCGCGCCCAGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAg                                             >  7:85069/1‑149 (MQ=255)
                                                                                                           aaCGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATc                                      >  3:301001/1‑149 (MQ=255)
                                                                                                           aaCGACAGGTACCGCACCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATc                                      >  3:321938/1‑149 (MQ=255)
                                                                                                              gACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGc                                   <  4:301001/149‑1 (MQ=255)
                                                                                                              gACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGc                                   >  7:295675/1‑149 (MQ=255)
                                                                                                                 aGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCAc                                <  7:27271/149‑1 (MQ=255)
                                                                                                                   gTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGt                              >  4:166851/1‑149 (MQ=255)
                                                                                                                   gTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGt                              <  1:198769/149‑1 (MQ=255)
                                                                                                                      ccGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGa                           <  3:166851/149‑1 (MQ=255)
                                                                                                                      ccGCGCCCGGCGGATCTGCTCCCAGACCAGAAGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTc                             >  5:210924/1‑147 (MQ=255)
                                                                                                                       cgcgCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAg                          >  6:51102/1‑149 (MQ=255)
                                                                                                                         ctgacggcgcATCTGGTCTGGGAGCAGATCCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGt                         >  5:263654/5‑148 (MQ=38)
                                                                                                                           cccGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAAc                      >  5:243058/1‑149 (MQ=255)
                                                                                                                              ggcgcATCTGGTCTGGGAGCAGATCCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGa                                                                        >  2:141120/1‑96 (MQ=37)
                                                                                                                              ggcgcATCTGGTCTGGGAGCAGATCCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGa                                                                        <  1:141120/96‑1 (MQ=37)
                                                                                                                                 ggATCTGCTCCCAGACCAGATACGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTgatcg                <  6:273080/149‑1 (MQ=255)
                                                                                                                                        cTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTGATCGATCTCAt         >  2:172716/1‑149 (MQ=255)
                                                                                                                                         tCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAAc                      <  8:19149/135‑1 (MQ=255)
                                                                                                                                         tCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTGATCGATCTCATg        >  3:84173/1‑149 (MQ=255)
                                                                                                                                         tCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCAACCGCCACGTCGAGTAAc                      >  7:19149/1‑135 (MQ=255)
                                                                                                                                          cccAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTGATCGATCTCATGc       >  3:291769/1‑149 (MQ=255)
                                                                                                                                           ccagaccagaTGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTGATCGATCTCATGcc      >  7:95021/1‑149 (MQ=255)
                                                                                                                                            cagaccagaTGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTGATCGATCTCATGCcg     >  2:105901/1‑149 (MQ=255)
                                                                                                                                               accagaTGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTGATCGATCTCATGCcgtcg  <  6:243058/149‑1 (MQ=255)
                                                                                                                                               accagaTGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTGATCGATCTCATGCcgtcg  >  3:301830/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GCGGCTGGTTGTCTTTGTCCAGCAGGCGAGCGCGCACGCCTTCACTGAATTCAGGGTGGCGGCAACAGTTCAGGCTCATGCTGTATTCCATCTGGAACACCTGCGCCAACGACAGGTACCGCGCCCGGCGGATCTGCTCCCAGACCAGATGCGCCGTCAGCGGGCAACCCTCATGCAGACGGTGGCCGGCATCTGCCAGCAATGGGTCGTCATGCTGCTTGAGCCTTTCCAGCGCGCGCCAGGCTGAAGCCGCATCCGCCACGTCGAGTAACTGATCGATCTCATGCCGTCG  >  NC_002947/1611072‑1611363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑