| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,670,221 | C→T | 75.0% | intergenic (+37/‑6) | trmD → / → rplS | tRNA (guanosine(37)‑N1)‑methyltransferase TrmD/50S ribosomal protein L19 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,670,221 | 0 | C | T | 75.0% | 88.6 / 29.2 | 56 | intergenic (+37/‑6) | trmD/rplS | tRNA (guanosine(37)‑N1)‑methyltransferase TrmD/50S ribosomal protein L19 |
| Reads supporting (aligned to +/- strand): ref base C (7/7); new base T (20/22); total (27/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
CCTTGGTAGGACCTTCGAACGACGCGCCGATCTTCTGGAAAGTCGCTCGCTTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGCACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTGAAGGAAGGTGAGCGTTCCCGTCTGCAGGCGTTCGAAGGC > NC_002947/1670076‑1670364 | ccTTGGTAGGACCTTCGAACGACGCGCCGATCTTCTGGAAAGTCGCTCGCTTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGtaca < 3:9389/149‑1 (MQ=255) ttGGTAGGACCTTCGAACGACGCGCCGATCTTCTGGAAAGTCGCTCGCTTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAg > 5:83972/1‑148 (MQ=255) ttCGAACGACGCGCCGATCTTCTGGAAAGTCGCTCGCTTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACcaac > 4:238766/1‑147 (MQ=255) cGAACGACGCTCCGATCTTCTGGAAAGTCGCTCGCTTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACcaac‑aa < 1:301262/147‑1 (MQ=255) cGAACGACGCGCCGATCTTCTGGAAAGTCGCTCGCTTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACcaac‑aa < 2:169885/147‑1 (MQ=255) aaCGACGCGCCGATCTTCTGGAAAGTCGCTCGCTTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGAACGAC‑AAGat > 1:113538/1‑148 (MQ=255) acgacgCGCCGATCTTCTGGAAAGTCGCTCGCTTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGat‑c > 6:176852/1‑148 (MQ=255) acgCGCCGATCTTCTGGAAAGTCGCTCGCTTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGCACAGCATGACCAAC‑AAGat‑catc > 5:203169/1‑148 (MQ=255) acgCGCCGATCTTCTGGAAAGTCGCTCGCTTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGCACAGCATGACCAAC‑AAGAT‑CATcc < 6:203169/149‑1 (MQ=255) cgcgCCGATCTTCTGGAAAGTCGCTCGCTTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCca < 8:203049/149‑1 (MQ=255) gcgcCGATCTTCTGGAAAGTCGCTCGCTTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGCACAGCATGACCAAC‑AAGAT‑CATCcag > 2:110733/1‑149 (MQ=255) tctGGAAAGTCGCTCGCTTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAg > 1:315710/1‑149 (MQ=255) gAAAGTCGCTCGCTTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGCACAGCATGACcaac > 4:24999/1‑123 (MQ=255) gAAAGTCGCTCGCTTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGCACAGCATGACcaac < 3:24999/123‑1 (MQ=255) agaGTCGCTCGCTTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAg > 2:183086/3‑149 (MQ=255) aaGTCGCTCGCTTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAg < 3:238766/148‑1 (MQ=255) gctcgctTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGAt < 6:83972/148‑1 (MQ=255) gctcgctTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGCGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGAt > 8:17816/1‑148 (MQ=255) gctTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAg < 3:283483/147‑1 (MQ=255) tCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGATCCATCCAGCAGCTCGAAGCCGAGCAGATGAGCaa < 1:207036/147‑1 (MQ=255) gagaagagaaGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGcc < 3:257537/128‑1 (MQ=255) gagaagagaaGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGcc > 4:257537/1‑128 (MQ=255) gagaagagaaGAAGCTGCGCGAGGAATATCTCAGCGAGCGGTAAGATAGTTAAAAGTATCGATTGAGCATAGCGTATACATCTTAGTAGTACATCATGCACAAC‑AAGAT‑CATCCAGCAGCTCGACGACGACCAGATGAGCAACGAAA‑ta > 5:140038/1‑148 (MQ=255) agaagagaagAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑Tc < 7:17816/148‑1 (MQ=255) gagaagaAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGa > 7:188758/1‑148 (MQ=255) gagaagaAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGAc > 8:162368/1‑149 (MQ=255) ctgctCGAGGAATATCTCCGCGAGCGGGACGATAGTAAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGCACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCAc > 1:262508/1‑148 (MQ=255) cGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGCACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCAGAGCAGATGAGAAAGGAAA‑TCCAGACCTTCGCACCAGGCg > 8:288474/1‑149 (MQ=255) gAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGa > 7:213083/1‑149 (MQ=255) ggAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGa > 7:303661/1‑123 (MQ=255) ggAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGa < 8:303661/123‑1 (MQ=255) tcCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTc > 2:81952/1‑149 (MQ=255) cgctAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTc < 7:257675/147‑1 (MQ=255) cgAGGGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATGCATCTTAGGAGCACAGCATGACGTAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCAGCAGGCGACACCGTTGTCGTTc < 2:262508/149‑1 (MQ=255) gggACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGCACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGtt < 7:288474/149‑1 (MQ=255) gATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGCACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTaa < 6:122723/146‑1 (MQ=255) gATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGCACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTaa > 5:122723/1‑146 (MQ=255) ttAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTgaag > 8:125528/1‑148 (MQ=255) aaCGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAATTCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTgaagga < 1:81952/148‑1 (MQ=255) aaCGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTgaaggaag < 7:133187/149‑1 (MQ=255) cGTATCTATGGTGGATCGCGTATCAATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGGGAAACCGTAGTCGTTCAGGTTAAAGTGAAGGAAGGt < 1:183086/149‑1 (MQ=255) cGTATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTGAAGGAAGGt > 3:97695/1‑149 (MQ=255) tATCGATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTGAAGGAAGGTGa < 8:188758/149‑1 (MQ=255) gATGGTGGATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTGAAGGAAGGTGCGCGt > 8:195200/1‑149 (MQ=255) aTGGTGGATCGCGTATCCATCTTAGGAGCACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTGAAGGAAGGTGAGc > 5:254867/1‑146 (MQ=255) aTGGTGGATCGCGTATCCATCTTAGGAGCACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTGAAGGAAGGTGAGc < 6:254867/146‑1 (MQ=255) tggtggATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTGAAGGAAGGTGAGCGTTc < 7:125528/149‑1 (MQ=255) tggATCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTGAAGGAAGGTGAGCGTTccc < 7:195200/148‑1 (MQ=255) gATCGCGTATCCATCTTAGGAGTACAGCATGACCAACTAAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTGAAGGAAGGTGAGCGTTCCCGt > 1:225312/1‑149 (MQ=255) aTCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTGAAGGAAGGTGAGCGTTCCCGTCt < 5:176852/149‑1 (MQ=255) tCGCGTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTGAAGGAAGGTGAGCGTTCCCGTCTg < 2:315710/149‑1 (MQ=255) gTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTGAAGGAAGGTGAGCg > 7:267120/1‑135 (MQ=255) gTATCCATCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTGAAGGAAGGTGAGCg < 8:267120/135‑1 (MQ=255) tCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTGAAGGAAGGTGAGCGTTCCCGTCTGCa < 5:193670/140‑1 (MQ=255) tCTTAGGAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTGAAGGAAGGTGAGCGTTCCCGTCTGCa > 6:193670/1‑140 (MQ=255) aGGAGCACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTGAAGGAAGGTGAGCGTTCCCGTCTGCAGGCGTTCGAAgg < 4:232190/148‑1 (MQ=255) ggAGTACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTGAAGGAAGGTGAGCGTTCCCGTCTGCAGGCGTTCGAAGGc < 8:213083/148‑1 (MQ=255) | CCTTGGTAGGACCTTCGAACGACGCGCCGATCTTCTGGAAAGTCGCTCGCTTTCTGGAGAAGAGAAGAAGCTGCTCGAGGAATATCTCCGCGAGCGGGACGATAGTTAAACGTATCGATGGTGGATCGCGTATCCATCTTAGGAGCACAGCATGACCAAC‑AAGAT‑CATCCAGCAGCTCGAAGCCGAGCAGATGAGCAAGGAAA‑TCCCGACCTTCGCACCAGGCGACACCGTTGTCGTTCAGGTTAAAGTGAAGGAAGGTGAGCGTTCCCGTCTGCAGGCGTTCGAAGGC > NC_002947/1670076‑1670364 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |