Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,715,647 T→C 7.8% V198A (GTG→GCG)  PP_1510 → carboxyvinyl‑carboxyphosphonate phosphorylmutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,715,6470TC 7.8% 138.4 / 2.8 51V198A (GTG→GCG) PP_1510carboxyvinyl‑carboxyphosphonate phosphorylmutase
Reads supporting (aligned to +/- strand):  ref base T (22/25);  new base C (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

TTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGTTGGCTCGTCGCTGGCCCGTGCTGCACTCGGGGCCTTCTAC  >  NC_002947/1715502‑1715792
                                                                                                                                                 |                                                                                                                                                 
ttCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCgtggt                                                                                                                                                <  2:148692/149‑1 (MQ=255)
   aCCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGTCGTGGTgca                                                                                                                                             <  4:225743/149‑1 (MQ=255)
   aCCTAGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCgtggtg                                                                                                                                               >  3:285824/1‑147 (MQ=255)
    ccTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTgcag                                                                                                                                            >  7:118588/1‑149 (MQ=255)
     cTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTgcagg                                                                                                                                           >  5:271187/1‑149 (MQ=255)
       tgtgCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTgcagg                                                                                                                                           >  2:195433/1‑147 (MQ=255)
         tgCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTgcaggcag                                                                                                                                        <  8:217453/148‑1 (MQ=255)
         tgCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGt                                                                                                                                       <  5:321226/149‑1 (MQ=255)
                 ggCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACcgc                                                                                                                               <  7:286513/149‑1 (MQ=255)
                       aaaCCTGCTTCACGGACGCATGGACCTCGACGACAAAATCCTGCGACTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCAGGGCTGCGCACCGTGGAGGAAGTACGTGCCGTGGTGCATGCAGTCGCACCGCGCCCtg                                                                                                                         >  2:275385/1‑149 (MQ=255)
                               tgcACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAAtgtg                                                                                                                 >  6:225648/1‑149 (MQ=255)
                                 cACGGACGCATGGACCTCGACGACCCCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCt                                                                                                               <  3:26823/149‑1 (MQ=255)
                                 cACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGc                                                                                                                <  5:225648/148‑1 (MQ=255)
                                       cGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCGCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCTCCCTGTGAATGTGCTGATggg                                                                                                         <  1:275385/149‑1 (MQ=255)
                                          aTGGACCTCGACGACACCATCCAGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCAt                                                                                                      >  1:29425/1‑149 (MQ=255)
                                                 tCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGACGGGCATggcgggc                                                                                               >  1:195280/1‑149 (MQ=255)
                                                   gacgacACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATggcgggc                                                                                               <  4:285824/147‑1 (MQ=255)
                                                       acacCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCg                                                                                         <  5:38315/149‑1 (MQ=255)
                                                           cATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCt                                                                                       >  7:101636/1‑147 (MQ=255)
                                                           cATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGa                                                                                     <  4:268560/149‑1 (MQ=255)
                                                             tCCTGCGCCTGCAAGCCTATGCGGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTGCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGc                                                                                   <  8:118588/149‑1 (MQ=255)
                                                                  cgcCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTc                                                                                <  1:195433/147‑1 (MQ=255)
                                                                  cgcCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCaa                                                                              <  1:40994/149‑1 (MQ=255)
                                                                      tGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAg                                                                          >  8:229236/1‑149 (MQ=255)
                                                                       gCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGACAAc                                                                             >  8:129878/1‑145 (MQ=255)
                                                                           gCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCtgt                                                                                                                        >  1:282199/1‑98 (MQ=255)
                                                                           gCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCtgt                                                                                                                        <  2:282199/98‑1 (MQ=255)
                                                                                tGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGAcc                                                                <  6:271187/149‑1 (MQ=255)
                                                                                   cGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTgg                                                             >  6:23519/1‑149 (MQ=255)
                                                                                       gCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGACGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGt                                                         <  2:195280/149‑1 (MQ=255)
                                                                                         cGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGGCAACCAGTTGCAGGACCTGGGCGTgc                                                       >  2:88186/1‑149 (MQ=255)
                                                                                                   gTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAg                                             >  4:21170/1‑149 (MQ=255)
                                                                                                         tACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCTTGCGCCGCGTCAGCGTTgg                                       >  8:284232/1‑149 (MQ=255)
                                                                                                         tACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGtt                                         <  2:29425/147‑1 (MQ=255)
                                                                                                         tACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGtt                                         <  7:229236/147‑1 (MQ=255)
                                                                                                         tACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGCGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGTTgg                                       >  5:107941/1‑149 (MQ=255)
                                                                                                           cGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGt                                          <  4:216803/144‑1 (MQ=255)
                                                                                                           cGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGt                                          >  3:216803/1‑144 (MQ=255)
                                                                                                             cccccGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGTTGGCtcg                                   >  1:4007/1‑149 (MQ=255)
                                                                                                              ccccGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGTTGGCtcgt                                  >  4:183165/1‑149 (MQ=255)
                                                                                                               cccGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGTTGGc                                      >  6:72788/1‑144 (MQ=255)
                                                                                                               cccGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGTTGGc                                      <  5:72788/144‑1 (MQ=255)
                                                                                                                         gcACCGTGGAGGAAGTCCGTGCCGCGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGTTGGCTCGTCGCTGGCCCGt                       >  8:74579/1‑149 (MQ=255)
                                                                                                                             cGTGGAGGAAGTCCGTGCCGTGGTGCAGGCTGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGtt                                                                        >  1:27020/1‑96 (MQ=255)
                                                                                                                             cGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGtt                                                                        <  2:27020/96‑1 (MQ=255)
                                                                                                                               tGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGTTGGCTCGTCGCTGGCCCGTGCTGCa                 <  8:101636/149‑1 (MQ=255)
                                                                                                                                  aggaAGTCCGTGCCGCGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGTTGGCTCGTCGCTGGCCCGTGCTGCACTc              <  6:107941/149‑1 (MQ=255)
                                                                                                                                   ggaAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGTTGGCTCGTCGCTGGCCCGtgct                    >  7:305256/1‑142 (MQ=255)
                                                                                                                                   ggaAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGTTGGCTCGTCGCTGGCCCGtgct                    <  8:305256/142‑1 (MQ=255)
                                                                                                                                       gTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGTTGGCTCGTCGCTGGCCCGTGCTGCACTCggg           >  7:280267/1‑147 (MQ=255)
                                                                                                                                       gTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGTTGGCTCGTCGCTGGCCCGTGCTGCACTCggg           <  8:280267/147‑1 (MQ=255)
                                                                                                                                       gTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAAGGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGTTGGCTCGTCGCTGGCCCGTGCTGCACTCGGGGc         <  6:105967/149‑1 (MQ=255)
                                                                                                                                        tCCGTGCCGCGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGTTGGCTCGTCGCTGGCCCGTGCTGCACTCGGGGcc        <  7:74579/149‑1 (MQ=255)
                                                                                                                                               cgtgGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTgcgc                                                     >  3:207061/1‑97 (MQ=255)
                                                                                                                                               cgtgGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTgcgc                                                     <  4:207061/97‑1 (MQ=255)
                                                                                                                                               cgtgGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGTTGGCTCGTCGCTGGCCCGTGCTGCACTCGGGGCCTTCTAc  >  7:14552/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TTCACCTTGTGCGCCCGGGCAGAAAACCTGCTGCACGGACGCATGGACCTCGACGACACCATCCTGCGCCTGCAAGCCTATGCCGAAGCCGGTGCCGACGTGCTCTACGCCCCCGGGCTGCGCACCGTGGAGGAAGTCCGTGCCGTGGTGCAGGCAGTCGCACCGCGCCCTGTGAATGTGCTGATGGGCATGGCGGGCGTGCCGCTGAGCGTCAACCAGTTGCAGGACCTGGGCGTGCGCCGCGTCAGCGTTGGCTCGTCGCTGGCCCGTGCTGCACTCGGGGCCTTCTAC  >  NC_002947/1715502‑1715792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑