Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,726,488 A→T 7.8% L559Q (CTG→CAG)  plsB ← glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,726,4880AT 7.8% 185.1 / 5.6 64L559Q (CTG→CAG) plsBglycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (30/29);  new base T (3/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.08e-01

CCGCGTTGCCTTCATCCAGGTAGAGGATGCGGCCCAGGGCGTCCTTCTGCTCGGCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATGGCGGCCGCCTCGTTGAGGTGGCGGGCCACGGTTTCTCCCAGGCGGG  >  NC_002947/1726342‑1726635
                                                                                                                                                  |                                                                                                                                                   
ccGCGTTGCCTTCATCCAGGTAGAGGATGCGGCCCAGGGCGTCCTTCTGCTCGGCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCagcagc                                                                                                                                                   >  8:252547/1‑149 (MQ=255)
     ttGCCTTCATCCAGGTAGAGGATGCGGCCCAGGGCGTCCTTCTGCTCGGCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCTGCTGCGCCaa                                                                                                                                              <  3:291955/149‑2 (MQ=255)
     ttGCCTTCATCCAGGTAGAGGATGCGGCCCAGGGCGTCCTTCTGCTCGGCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAg                                                                                                                                              >  3:13602/1‑149 (MQ=255)
        ccTTCATCCAGGTAGAGGATGCGGCCCTGGGCGTCCTTCTGCTCGGCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGAGAGTAGGGCACTTGGCGCAGCAGCGCGAGGTa                                                                                                                                           >  6:7837/1‑149 (MQ=255)
             aTCCAGGTAGAGGATGCGGCCCAGGGCGTCCTTCTGCTCGGCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGt                                                                                                                                      >  8:272983/1‑149 (MQ=255)
              tCCAGGTAGAGGATGCGGCCCAGGGCGTCCTTCTGCTCGGCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGGAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTc                                                                                                                                     >  1:268162/1‑149 (MQ=255)
                cAGGTAGAGGATGCGGCCCAGGGCGTCCTTCTGCTCGGCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTc                                                                                                                                     >  7:232802/1‑147 (MQ=255)
                              ggCCCAGGGCGTCCTTCTGCTCGGCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAgg                                                                                                                     <  2:34406/149‑1 (MQ=255)
                                cccAGGGCGTCCTTCTGCTCGGCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGgc                                                                                                                   >  8:97160/1‑149 (MQ=255)
                                cccAGGGCGTCCTTCTGCTCGGCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGgc                                                                                                                   <  5:173869/149‑1 (MQ=255)
                                  cAGGGCGTCCTTCTGCTCGGCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGgcgc                                                                                                                 <  8:75132/149‑1 (MQ=255)
                                   aGGGCGTCCTTCTGCTCGGCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAg                                                                                                                      >  1:325822/1‑143 (MQ=255)
                                   aGGGCGTCCTTCTGCTCGGCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAg                                                                                                                      <  2:325822/143‑1 (MQ=255)
                                           cTTCTGCTCGGCCACCAGGTTCATGCTGAGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTc                                                                                                          >  1:19158/1‑147 (MQ=255)
                                              cTGCTCGGCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAgg                                                                                                      <  6:135533/148‑1 (MQ=255)
                                              cTGCTCGGCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAg                                                                                                       <  7:72272/147‑1 (MQ=255)
                                               tGCTCGGCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAg                                                                                                       <  5:304413/146‑1 (MQ=255)
                                               tGCTCGGCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAg                                                                                                       >  6:304413/1‑146 (MQ=255)
                                                 cTCGGCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGc                                                                                                    <  2:19158/147‑1 (MQ=255)
                                                    ggCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCGGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAgg                                                                                                <  5:191797/148‑1 (MQ=255)
                                                       caccaGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAggcggc                                                                                            >  4:304867/1‑149 (MQ=255)
                                                        accaGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCt                                                                                           >  1:35532/1‑149 (MQ=255)
                                                         ccaGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCtg                                                                                          >  7:126811/1‑149 (MQ=255)
                                                          caGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGgcgcgc                                                                                                               <  4:225595/127‑1 (MQ=255)
                                                          caGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCtgg                                                                                         <  7:252547/149‑1 (MQ=255)
                                                           aGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCtggt                                                                                        <  7:272983/149‑1 (MQ=255)
                                                            ggTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCtggtg                                                                                       <  4:131648/149‑1 (MQ=255)
                                                                       cgcACATCTTCGATCAGTGCCTGGCCGTCGCCCGCGGGCAGGGGGGGGTGCGGCGAGTCGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGt                                                                              <  5:145/147‑1 (MQ=255)
                                                                        gcACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGc                                                                            >  3:84742/1‑148 (MQ=255)
                                                                          acaTGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACATGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAg                                                                         >  1:95649/1‑149 (MQ=255)
                                                                           caTGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAgg                                                                        <  7:97160/149‑1 (MQ=255)
                                                                              gTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGc                                                                      >  2:87229/1‑148 (MQ=255)
                                                                               ttCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGcca                                                                    >  1:194901/1‑149 (MQ=255)
                                                                                  gATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAAGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGccacc                                                                  <  1:87229/148‑1 (MQ=255)
                                                                                      aGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGAcagca                                                                                <  3:267090/130‑1 (MQ=255)
                                                                                      aGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGAcagca                                                                                >  4:267090/1‑130 (MQ=255)
                                                                                      aGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGtt                                                             <  4:13602/149‑1 (MQ=255)
                                                                                       gTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGtt                                                             >  8:18777/1‑148 (MQ=255)
                                                                                        tGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGa                                                           >  7:84328/1‑149 (MQ=255)
                                                                                        tGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGa                                                           >  7:97520/1‑149 (MQ=255)
                                                                                              gCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCggg                                                      <  8:232802/148‑1 (MQ=255)
                                                                                               ccGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGtt                                                    <  5:106899/149‑1 (MQ=255)
                                                                                                cGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGtt                                                    >  5:94291/1‑148 (MQ=255)
                                                                                                     cccTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATggc                                              >  4:307559/1‑149 (MQ=255)
                                                                                                     cccTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATggc                                              >  4:302786/1‑149 (MQ=255)
                                                                                                     cccTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATggc                                              <  4:137045/149‑1 (MQ=255)
                                                                                                      ccTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACATGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATggcg                                             >  1:210129/1‑149 (MQ=255)
                                                                                                      acTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACATGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTACAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACAGGGTTGATggcg                                             >  1:214725/2‑149 (MQ=255)
                                                                                                           ggCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATGGCGgccgc                                        <  3:304867/149‑1 (MQ=255)
                                                                                                                ggtggtGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATGGCGGCCGCCGCGt                                   <  6:94291/149‑1 (MQ=255)
                                                                                                                 gtggtgTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATGGCGGCCGCCTCGtt                                  <  8:126811/149‑1 (MQ=255)
                                                                                                                      gtgCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATGGCGGCCGCCTCGTTGAGGt                             >  6:246801/1‑149 (MQ=255)
                                                                                                                         cggcgAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATGGCGGCCGCCTCGTTGAGGTGGc                          <  3:218370/149‑1 (MQ=255)
                                                                                                                           gctaGTAGGGCACTTGGCGCAGCAGCGCCAGGTACATGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGTCAAGTCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATGGCGGCCGACTCGTTGAGGTGGCgg                        <  2:214725/146‑1 (MQ=255)
                                                                                                                           gcgAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATGGCGGCCGCCTCGTTGAGGTGGCgg                        <  2:210129/149‑1 (MQ=255)
                                                                                                                                  gggCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATGGCGGCCGCCTCGTTGAGGTGGCGGGCCACg                  <  2:35532/148‑1 (MQ=255)
                                                                                                                                   ggCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACGGGGTTGATGGCGGCCGCCTCGTTGAGGTGGCGGGCCACGGt                >  5:283687/1‑149 (MQ=255)
                                                                                                                                     cACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCACGGCGGCTGGTGGACAGCAGTGCCATGGACACCAGGTTGACCGGGTTGATGGCGGCCGCCGCGTTGAGGTGCCGGGCCACGGttt              >  3:184998/1‑149 (MQ=255)
                                                                                                                                     cACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATGGCGGCCGCCTCGTTGAGGTGGCGGGCCACGGttt              >  6:218006/1‑149 (MQ=255)
                                                                                                                                      aCTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATGGCGGCCGCCTCGTTGAGGTGGCGGGCCACGGTTtc             >  1:136518/1‑149 (MQ=255)
                                                                                                                                        ctGGCGCTGCTGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATggcg                                             <  5:181885/114‑1 (MQ=255)
                                                                                                                                        ctGGCGCTGCTGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATggcg                                             >  6:181885/2‑115 (MQ=255)
                                                                                                                                        ctGGCGCTGCTGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATGGCGGCCGCCTCGTTGAGGTGGCGGGCCACGGTTtctc           >  4:75613/2‑149 (MQ=255)
                                                                                                                                        ctGGCGCTGCTGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATGGCGGCCGCCTCGTTGAGGTGGCGGGCCACGGTTtctc           >  7:111829/2‑149 (MQ=255)
                                                                                                                                           gcgcAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATGGCGGCCGCCTCGTTGAGGTGGCGGGCCACGGTTTCTCCCa        <  2:95649/149‑1 (MQ=255)
                                                                                                                                                 cagcGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATGGCGGCCGCCTCGTTGAGGTGGCGGGCCACGGTTTCTCCCAggcggg  >  1:12973/1‑149 (MQ=255)
                                                                                                                                                 cagcGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATGGCGGCCGCCTCGTTGAGGTGGCGGGCCACGGTTTCTCCCAggcggg  <  7:18777/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CCGCGTTGCCTTCATCCAGGTAGAGGATGCGGCCCAGGGCGTCCTTCTGCTCGGCCACCAGGTTCATGCTGCGCACATGTTCGATCAGTGCCTGGCCGTCGCCCTCGGGCAGGGTGGTGTGCGGCGAGTAGGGCACTTGGCGCAGCAGCGCCAGGTACAGGTCGAGTACGCGGGTCAGGGCGCGCTCGTCCAGGGCAAGGCGGCTGGTGGACAGCAGTGCCAGGGCCACCAGGTTGACCGGGTTGATGGCGGCCGCCTCGTTGAGGTGGCGGGCCACGGTTTCTCCCAGGCGGG  >  NC_002947/1726342‑1726635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑