Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,731,535 G→C 11.0% R779G (CGC→GGC)  PP_1526 ← beta‑(1‑3)‑glucosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,731,5350GC11.0% 152.1 / 6.6 54R779G (CGC→GGC) PP_1526beta‑(1‑3)‑glucosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (27/20);  new base C (2/3);  total (31/23)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.55e-01

GCGCATGTCCGAACTCGGCAGCCCCTGCACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAGGAAGATGATCTTGCCGACC  >  NC_002947/1731395‑1731683
                                                                                                                                            |                                                                                                                                                    
gcgcATGTCCGAACTCGGCAGCCCCTGCACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCCGCACGCCGAAGAACg                                                                                                                                              <  1:254135/149‑1 (MQ=255)
gcgcATGTCCGAACTCGGCAGCCCCTGCACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCCGCACGCCGAAGAACg                                                                                                                                              <  5:307755/149‑1 (MQ=255)
   cATGTCCGAACTCGGCAGCCCCTGCACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCa                                                                                                                                           <  7:216280/149‑1 (MQ=255)
       tCCGAACTCGGCAGCCCCTGCACCAGGTAGATGCCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCAtgct                                                                                                                                       >  7:142047/1‑149 (MQ=255)
       tCCGAACTCGGCAGCCCCTGCACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCAtgct                                                                                                                                       >  7:228088/1‑149 (MQ=255)
       tCCGAACTCGGCAGCCCCTGCACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCAtgct                                                                                                                                       >  6:30827/1‑149 (MQ=255)
          gAACTCGGCAGCCCCTGCACCAGGTAGATAGCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCAtgctgc                                                                                                                                     >  1:30176/1‑148 (MQ=255)
          gAACTCGGCAGCCCCTGCACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCAtgctgc                                                                                                                                     <  2:30176/148‑1 (MQ=255)
                           cACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATa                                                                                                                     <  4:162465/147‑1 (MQ=255)
                              cAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCCGCACGCCGAAGAACg                                                                                                                                              <  1:139414/119‑1 (MQ=255)
                              cAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCCGCACGCCGAAGAACg                                                                                                                                              >  2:139414/1‑119 (MQ=255)
                               aGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTAc                                                                                                               >  1:221269/1‑149 (MQ=255)
                                         ccAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGctgctg                                                                                                                                   <  4:227863/119‑1 (MQ=255)
                                         ccAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGctgctg                                                                                                                                   >  3:227863/1‑119 (MQ=255)
                                         ccAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATAc                                                                                                                    <  6:262897/134‑1 (MQ=255)
                                         ccAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATAc                                                                                                                    >  5:262897/1‑134 (MQ=255)
                                         ccAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGc                                                                                                      >  4:138326/1‑148 (MQ=255)
                                            aCGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATg                                                                                                  <  5:30827/149‑1 (MQ=255)
                                              gccgcGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGgt                                                                                                >  6:301665/1‑149 (MQ=255)
                                                cgcgCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGgtgt                                                                                              <  2:14079/149‑1 (MQ=255)
                                                 gcgcCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATa                                                                                                                     <  2:68283/125‑1 (MQ=255)
                                                 gcgcCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATa                                                                                                                     >  1:68283/1‑125 (MQ=255)
                                                     cccACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGa                                                                                          >  5:213031/1‑148 (MQ=255)
                                                       cacaGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTAcc                                                                                                              >  4:33008/1‑126 (MQ=255)
                                                       cacaGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTAcc                                                                                                              <  3:33008/126‑1 (MQ=255)
                                                       cacaGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAg                                                                                       >  3:175550/1‑149 (MQ=255)
                                                        acaGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGt                                                                                      <  8:142047/149‑1 (MQ=255)
                                                              gcaCCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGGGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGAAGGTGTGCGAAAGTGCCAGc                                                                                >  2:96144/1‑149 (MQ=255)
                                                                        gaACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGAGACAGTGACAGCCCGGCCAgcg                                                                      >  4:116928/1‑149 (MQ=255)
                                                                          aCAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGt                                                                                      >  6:166967/1‑131 (MQ=255)
                                                                          aCAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGt                                                                                      <  5:166967/131‑1 (MQ=255)
                                                                              ctctTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTg                                                                >  5:186779/1‑149 (MQ=255)
                                                                                ctTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGc                                                               >  5:294506/1‑148 (MQ=255)
                                                                                  tCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCg                                                              >  1:83628/1‑147 (MQ=255)
                                                                                   cACGGGCTTCGGAAATCGCCACCAGCAACGGTTGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTTAAGAAACCATACAGTACCGCCTTGGCGTTGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGa                                                             <  8:197190/147‑1 (MQ=255)
                                                                                            cGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCtt                                                   <  2:83628/148‑1 (MQ=255)
                                                                                              gAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAg                                                >  2:161050/1‑149 (MQ=255)
                                                                                                       accaGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGCAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCAcccc                                       >  3:181627/1‑149 (MQ=255)
                                                                                                                ccATGGCTGTCAGCGTTCTTCGGCGTGCAGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTcg                              >  1:183018/1‑149 (MQ=255)
                                                                                                                ccATGGCTGTCAGCGTTCTTCGGCGTGCAGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTCGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTcg                              >  1:188076/1‑149 (MQ=255)
                                                                                                                  aTGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTcggc                            >  8:168991/1‑149 (MQ=255)
                                                                                                                     gCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTcggcgg                          <  2:221269/148‑1 (MQ=255)
                                                                                                                      cTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTa                        <  3:138326/149‑1 (MQ=255)
                                                                                                                         tCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACa                      <  7:168991/148‑1 (MQ=255)
                                                                                                                           aGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATcc                                                     <  5:33488/115‑1 (MQ=255)
                                                                                                                           aGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATcc                                                     >  6:33488/1‑115 (MQ=255)
                                                                                                                           aGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAgg                    >  4:44388/1‑148 (MQ=255)
                                                                                                                           aGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAgg                    >  3:208917/1‑148 (MQ=255)
                                                                                                                            gCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAgg                    >  4:119288/1‑147 (MQ=255)
                                                                                                                             cGTTCTTCCGCACGCCGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAgg                    >  6:58970/1‑146 (MQ=255)
                                                                                                                                  ttcGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAGGAAgatgat            >  2:228504/1‑149 (MQ=255)
                                                                                                                                    cGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAGGAAGATGATc           <  8:228088/148‑1 (MQ=255)
                                                                                                                                     ggCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAGGAAGATGATCtt         <  4:175550/149‑1 (MQ=255)
                                                                                                                                           cGGATGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGTTGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAGGAAGATGATCTTGCCGa    <  3:116928/148‑1 (MQ=255)
                                                                                                                                            ggAAGAACGGCATGCTGCTGGTGAAGAAACGATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAGGAAGATGATCTTGCCGAcc  >  1:28943/1‑149 (MQ=255)
                                                                                                                                            ggAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAGGAAGATGATCTTGCCGAcc  <  1:161050/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
GCGCATGTCCGAACTCGGCAGCCCCTGCACCAGGTAGATACCCAACGCCGCGCCCCACAGCAGCACCATGATGAACAGCTCTTCACGGGCTTCGGAAATCGCCACCAGCAACCCATGGCTGTCAGCGTTCTTCGGCGTGCGGAAGAACGGCATGCTGCTGGTGAAGAAACCATACAGTACCGCCTTGGCGATGGTGTGCGACAGTGCCAGCCCGGCCAGCGCAGCTGCGAAGGCATCCTTGAGGTTCACCCCCACCGCTCGGCGGTACAGGAAGATGATCTTGCCGACC  >  NC_002947/1731395‑1731683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑