Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,817,333 G→A 9.1% A159A (GCG→GCA)  pcm → protein‑L‑isoaspartate O‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,817,3330GA 9.1% 120.6 / 2.9 44A159A (GCG→GCA) pcmprotein‑L‑isoaspartate O‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (17/23);  new base A (2/2);  total (19/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

GACGGCGATCCTGGCCCAGCTGGTCGAGCGAGTGTTTTCGGTAGAGCGTATCAAGGTACTGCAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGACCAACTGGCACCCGGTGGGCGCATGGTCATCCCGGTAGGGCCGGCCGGCGAAGCGCAGCAACTGATGCTGAT  >  NC_002947/1817186‑1817470
                                                                                                                                                   |                                                                                                                                         
gACGGCGATCCTGGCCCAGCTGGTCGAGCGAGTGTTTTCGGTAGAGCGTATCAAGGTACTGCAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCTCTGGGGTGACGGTGGTGCTGGCTGGCCGgcgc                                                                                                                                          >  6:228600/1‑149 (MQ=255)
     cGATCCTGGCCCAGCTGGTCGAGCGAGTGTTTTCGGTAGAGCGTATCAAGGTACTGCAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTgcg                                                                                                                                     >  4:308559/1‑149 (MQ=255)
      gATCCTGGCCCAGCTGGTCGAGCGAGTGTTTTCGGTAGAGCGTATCAAGGTACTGCAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTgcgc                                                                                                                                    <  2:191618/149‑1 (MQ=255)
               ccAGCTGGTCGAGCGAGTGTTTTCGGTAGAGCGTATCAAGGTACTGCAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACg                                                                                                                           >  3:19488/1‑149 (MQ=255)
                      gTCGAGGGTGTGTTTTCGGTAGAGCGTATGAAGGTAGTGCAGGACCGGGCCATAGAGCGCCTGGTGGAGCTTAAGCTGCGCTACGTGGTCTTCCGCTGGGGTGAGGGTTGTGAGGGGTGGGCGGCGCTTGCGCCGTACAACGGcatg                                                                                                                      <  1:162525/147‑2 (MQ=255)
                      gTCGAGCGAGTGTTTTCGGTAGAGCGTATCAAGGTACTGCAGGACCGGGCCAAATAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCAGCTGGGGTGACGGTTGTGAGTTGTGGCCGGCGCTTGCGCCGTACAACGGcatc                                                                                                                      <  1:167628/147‑1 (MQ=255)
                             gagTGTTTTCGGTAGAGCGTATCAAGGTACTGCAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCACTTGCGCCGTACAACGGCATCATCGTCAcc                                                                                                             >  4:169440/1‑149 (MQ=255)
                               gtgtTTTCGGTAGAGCGTATCAAGGTACTGCAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCAccgc                                                                                                           >  6:77950/1‑149 (MQ=255)
                               gtgtTTTCGGTAGAGCGTATCAAGGTACTGCAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCAccgc                                                                                                           >  6:179602/1‑149 (MQ=255)
                               gtgtTTTCGGTAGAGCGTATCAAGGTACTGCAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCAccgc                                                                                                           <  6:128745/149‑1 (MQ=255)
                                  ttttCGGTAGAGCGTATCAAGGTACTGCAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGGGAGGGGTGGACGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGt                                                                                                        >  8:233859/1‑149 (MQ=255)
                                        gTAGCGCGTATCAAGGTACGGCAGGACCGGGCCAAAGAGCGCGGGGTGGAGCTTAACGTGCGCAAGGTGGGCTTCGGCTGGGGTGTCGGATGGGGGGGGTGGCGGGGGCTTGCGCGGTCCAAGGGCAGCAGCGTGACCGCCGGGGCGcc                                                                                                  >  4:74079/1‑149 (MQ=255)
                                             gCGTATCAAGGTACTGCAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGaa                                                                                              <  4:150188/148‑1 (MQ=255)
                                              cGTATCAAGGTACTGCAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGt                                                                                            <  1:14770/149‑1 (MQ=255)
                                                 aTCAAGGTACTGCAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGc                                                                                          >  8:242716/1‑148 (MQ=255)
                                                   cAAGGTACTGCAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCAACGTCACCGCCGAGGCGCCGGAAGTGcaa                                                                                        >  6:334477/1‑146 (MQ=255)
                                                    aaGGTACTGCAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCcacc                                                                                      >  2:94826/1‑148 (MQ=255)
                                                      ggTACTGCAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAgg                                                                                    <  1:6968/149‑1 (MQ=255)
                                                       gTACTGCAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGcctgtggc                                                                                   <  5:77950/149‑7 (MQ=255)
                                                       gTACTGCAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCACTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAgg                                                                                    >  2:8637/1‑148 (MQ=255)
                                                          cTGCAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCAc                                                                                 <  2:234914/148‑1 (MQ=255)
                                                             cAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGc                                                                              <  3:308559/148‑1 (MQ=255)
                                                                gACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCACTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGa                                                                          <  1:8637/149‑1 (MQ=255)
                                                                     ggCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGACCAAc                                                                     >  8:234531/1‑149 (MQ=255)
                                                                         aaaGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGAGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGACCAACTgg                                                                  >  1:238955/1‑148 (MQ=255)
                                                                          aaGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGACCAACTGGCa                                                                >  6:209567/1‑149 (MQ=255)
                                                                               cgcCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCGCAGGCACTGCTCGACCAACTGGCACCCgg                                                           <  5:37347/149‑1 (MQ=255)
                                                                                gcCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGACCAACTGGCACCCGGt                                                          <  5:179602/149‑1 (MQ=255)
                                                                                gcCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGACCAACTGGCACCCGGt                                                          <  5:29360/149‑1 (MQ=255)
                                                                                     gtggAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAg                                                                                             >  1:139879/1‑109 (MQ=255)
                                                                                     gtggAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAg                                                                                             <  2:139879/109‑1 (MQ=255)
                                                                                         aGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGGGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGACCAACTGGCACCCGGTGGGCGCATg                                                 <  5:334477/149‑1 (MQ=255)
                                                                                                    cgcAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCTGAAGTGCCACAGGCACTGCTCGACCAACTGGCACCCGGTGGGCGCATGGTCATCCCg                                        <  7:242716/147‑1 (MQ=255)
                                                                                                    cgcAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGACCAACTGGCACCCGGTGGGCGCATGGTCATCCCg                                        <  4:19488/147‑1 (MQ=255)
                                                                                                      cAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCACTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGACCAACTGGCACCCGGTGGGCGCATGGTCATCCCGGTAg                                    <  3:169440/149‑1 (MQ=255)
                                                                                                         cGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGACCAACTGGCACCCGGTGGGCGCATGGTCATCCCGGTAGggc                                 <  7:234531/149‑1 (MQ=255)
                                                                                                                ttCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGACCAACTGGCACCCGGTGGGCGCATGGTCATCCCGGTAGGGccggccgg                          >  6:1805/1‑149 (MQ=255)
                                                                                                                  ccGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGACCAACTGGCACCCGGTGGGCGCATGGTCATCCCGGTAGGGccggccgg                          <  5:209567/147‑1 (MQ=255)
                                                                                                                  ccGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGACCAACTGGCACCCGGTGGGCGCATGGTCATCCCGGTAGGGccggccgg                          <  6:281678/147‑1 (MQ=255)
                                                                                                                      tGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGACCACCTGGCACCCGGTGGGCGCATGGTCATCCCGGTAGGGCCGGCCGGCGAAgc                    >  5:76582/1‑149 (MQ=255)
                                                                                                                          gTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGACCAACTGGCACCCGGTGGGCGCATGGTCATCCCGGTAGGGCCGGCCGGCGAAgcg                   >  7:24045/1‑146 (MQ=255)
                                                                                                                          gTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGACCAACTGGCACCCGGTGGGCGCATGGTCATCCCGGTAGGGCCGGCCGGCGAAgcg                   <  8:24045/146‑1 (MQ=255)
                                                                                                                           tGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGACCAACTGGCACCCGGTGGGCGCATGGTCATCCCGGTAGGGCCGGCCGGCgaagcgaagc               <  5:228600/149‑1 (MQ=255)
                                                                                                                                 ttGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGACCAACTGGCACCCGGTGGGCGCATGGTCATCCCGGTAGGGCCGGCCGGCGAAGCGCAGCAACTGa         <  3:74079/149‑1 (MQ=255)
                                                                                                                                        gggTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGACCAACTGGCACCCGGTGGGCGCATGGTCATCCCGGTAGGGCCGGCCGGCGAAGCGCAGCAACTGATGCTGAt  >  2:179120/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                         
GACGGCGATCCTGGCCCAGCTGGTCGAGCGAGTGTTTTCGGTAGAGCGTATCAAGGTACTGCAGGACCGGGCCAAAGAGCGCCTGGTGGAGCTTAACCTGCGCAACGTGGTCTTCCGCTGGGGTGACGGTTGTGAGGGGTGGCCGGCGCTTGCGCCGTACAACGGCATCATCGTCACCGCCGTGGCGCCGGAAGTGCCACAGGCACTGCTCGACCAACTGGCACCCGGTGGGCGCATGGTCATCCCGGTAGGGCCGGCCGGCGAAGCGCAGCAACTGATGCTGAT  >  NC_002947/1817186‑1817470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑