Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,823,180	C→T	100%	D692N (GAC→AAC)	mutS ←	DNA mismatch repair protein MutS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,823,180	0	C	T	100.0%	147.9 / NA	45	D692N (GAC→AAC)	mutS	DNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/24);  total (21/24)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCAGCGGCTCGCTCTCCGGCAGCACGGTCAGTTCGAAGTAGTGGGTGGCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTCCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCATCTCCACCATGAAGGTCGAGCGCCCGCCGGCCAGGTCGTCACTGGAGCCGATGCGGGTGAAGATGCGGTCGACCAGCGACAACTCG  >  NC_002947/1823036‑1823322                                                                                                                                                 |                                                                                                                                               ccaGCGGCTCGCTCTCCGGCAGCACGGTCAGTTCGAAGTAGTGGGTGGCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCAt                                                                                                                                             >  6:326230/1‑148 (MQ=255)    gCGGCTCGCTCTCCGGCAGCACGGTCAGTTCGAAGTAGTGGGTGGCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGAGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAg                                                                                                                                          >  3:164639/1‑148 (MQ=255)         tcgctcTCCGGCAGCACGGTCAGTTCGAAGTAGTGGGTGGCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAg                                                                                                                                    >  4:106211/1‑149 (MQ=255)           gctcTCCGGCAGCACGGTCAGTTCGAAGTAGTGGGTGGCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAg                                                                                                                                    <  5:169494/147‑1 (MQ=255)           gctcTCCGGCAGCACGGTCAGTTCGAAGTAGTGGGTGGCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGc                                                                                                                                  >  5:260343/1‑149 (MQ=255)               tcCGGCAGCACGGTCAGTTCGAAGTAGTGGGTGGCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGcg                                                                                                                              >  4:15286/1‑149 (MQ=255)               tcCGGCAGCACGGTCAGTTCGAAGTAGAGGGTGGCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTAGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGcg                                                                                                                              >  5:40152/1‑149 (MQ=255)                   gcagcaCGGTCAGTTCGAAGTAGTGGGTGGCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGcggtcg                                                                                                                          >  8:240823/1‑149 (MQ=255)                    cagcaCGGTCAGTTCGAAGTAGTGGGTGGCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGcggtcgg                                                                                                                         <  7:283154/149‑1 (MQ=255)                      gcaCGGTCAGTTCGAAGTAGTGGGTGGCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTg                                                                                                                       <  3:320858/149‑1 (MQ=255)                           gTCAGTTCGAAGTAGTGGGTGGCGAACAAGGTGTAGGCACGCAGCTGGGCCAGTCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCAtt                                                                                                                  <  6:40152/149‑1 (MQ=255)                            tCAGTTCGAAGTAGTGGGTGGCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATtg                                                                                                                 <  1:8976/149‑1 (MQ=255)                                ttCGAAGTAGTGGGTGGCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCt                                                                                                                                 >  5:76741/1‑129 (MQ=255)                                ttCGAAGTAGTGGGTGGCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCt                                                                                                                                 <  6:76741/129‑1 (MQ=255)                                ttCGAAGTAGTGGGTGGCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCa                                                                                                             <  5:326230/149‑1 (MQ=255)                                   gAAGTAGTGGGTGGCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGa                                                                                                          <  1:146464/149‑1 (MQ=255)                                         gtgggtggCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTgg                                                                                                    <  3:15286/149‑1 (MQ=255)                                         gtgggtggCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTgg                                                                                                    >  2:298841/1‑149 (MQ=255)                                              tggCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGCCAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCCGGCTGCGGTCGGGGGCATTGTGCAGGATGTTGGCGGtt                                                                                               >  7:264600/1‑149 (MQ=255)                                              tggCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGtt                                                                                               >  4:28609/1‑149 (MQ=255)                                                 cGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCg                                                                                            <  6:260343/149‑1 (MQ=255)                                                  gAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGc                                                                                           >  4:61155/1‑149 (MQ=255)                                                    aCAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTc                                                                                         <  7:240823/149‑1 (MQ=255)                                                    aCAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTc                                                                                         <  1:298841/149‑1 (MQ=255)                                                         gtgtAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCAtctc                                                                                    >  2:236051/1‑149 (MQ=255)                                                         gtgtAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCAtctc                                                                                    >  2:18795/1‑149 (MQ=255)                                                            tAGGCACGCAGCTTGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCTGTCGGTTGCATTGTGCATGATGTTGGCGGTTTCGCTCATCTccac                                                                                 <  4:314988/149‑1 (MQ=255)                                                            tAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCTACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCATCTccac                                                                                 <  8:299222/149‑1 (MQ=255)                                                                      gCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCATCTCCACCATGAAGGTc                                                                       <  3:106211/149‑1 (MQ=255)                                                                          ggCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCATCTCCACCATGAAGGTCGAg                                                                    <  2:143018/148‑1 (MQ=255)                                                                                gcgcTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCATCTCCACCATGAAGGTCGAGcgcccgc                                                             >  1:3358/1‑149 (MQ=255)                                                                                       gCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCATCTCCACCATGAAGGTCGAGCGCCCGCCGGCCAg                                                      <  3:28609/149‑1 (MQ=255)                                                                                        cGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCATCTCCACCATGAAGGTCGAGCGCCCGCCGGCCAgg                                                     <  3:61155/149‑1 (MQ=255)                                                                                         ggCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACAGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCATCTCCACCATGAAGGTCGAGCGCCCGCCGGCCAGgt                                                    >  8:47086/1‑149 (MQ=255)                                                                                                 aGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCATCTCCACCATGAAGGTCGAGCGCCCGCCGGCCAGGTCGTCACTg                                            <  1:236051/149‑1 (MQ=255)                                                                                                 aGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCATCTCCACCATGAAGGTCGAGCGCCCGCCGGCCAGGTCGTCACTg                                            <  1:18795/149‑1 (MQ=255)                                                                                                   gCCAGCGCCAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCATCTCCACCATGAAGGTCGAGCGCCCGCCggt                                                         >  5:102748/1‑133 (MQ=255)                                                                                                   gCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCATCTCCACCATGAAGGTCGAGCGCCCGCCggt                                                         <  6:102748/134‑2 (MQ=255)                                                                                                     cAGCGACAGGCCGTCGAAGGTGCTGGTACAGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCATCTCCACCATGAAGGTCGAGCGCCCGCCGGCCAGGTCGTCACTGGAGc                                        <  7:47086/149‑1 (MQ=255)                                                                                                            aGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCAGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCATCTCCACCATGAAGGTCGAGCGCCCGCCGGCCAGGTCGTCACTGGAGCCGATGCg                                 >  1:246522/1‑149 (MQ=255)                                                                                                                  tCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCATCTCCACCATGAAGGTCGAGCGCCCGCCGGCCAGGTCGTCACTGGAGCCGATGCGGGTGaa                           <  3:22668/149‑1 (MQ=255)                                                                                                                  tCGAAGGTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCATCTCCACCATGAAGGTCGAGCGCCCGCCGGCCAGGTCGTCACTGGAGCCGATGCGGGTGaa                           <  2:3358/149‑1 (MQ=255)                                                                                                                       ggTGCTGGTACCGCGGCCCACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCATCTCCACCATGAAGGTCGAGCGCCCGCCGGCCAGGTCGTCACTGGAGCCGATGCGGGTGAAGATGc                      >  6:95632/1‑149 (MQ=255)                                                                                                                                         cACTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCATCTCCACCATGAAGGTCGAGCGCCCGCCGGCCAGGTCGTCACTGGAGCCGATGCGGGTGAAGATGCGGTCGACCAGCGAcaact    >  7:102761/1‑149 (MQ=255)                                                                                                                                           cTTCGTTCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCATCTCCACCATGAAGGTCGAGCGCCCGCCGGCCAGGTCGTCACTGGAGCCGATGCGGGTGAAGATGCGGTCGACCAGCGAcaactcg  >  3:112511/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                               CCAGCGGCTCGCTCTCCGGCAGCACGGTCAGTTCGAAGTAGTGGGTGGCGAACAAGGTGTAGGCACGCAGCTGGGCCAGGCGCTCAGCGGCAGCCCAGGCCAGCGACAGGCCGTCGAAGGTGCTGGTACCGCGGCCCACTTCGTCCATCAGCACCAGGCTGCGGTCGGTGGCATTGTGCAGGATGTTGGCGGTTTCGCTCATCTCCACCATGAAGGTCGAGCGCCCGCCGGCCAGGTCGTCACTGGAGCCGATGCGGGTGAAGATGCGGTCGACCAGCGACAACTCG  >  NC_002947/1823036‑1823322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑