| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,905,820 | A→C | 7.0% | E692A (GAG→GCG) ‡ | nirB → | nitrite reductase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,905,820 | 0 | A | C | 7.0% | 172.6 / 4.4 | 57 | E692A (GAG→GCG) ‡ | nirB | nitrite reductase large subunit |
| Reads supporting (aligned to +/- strand): ref base A (26/26); new base C (2/2); total (28/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01 | |||||||||||
AAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCTGGTACGCCTGATCGACCGCG > NC_002947/1905675‑1905966 | aaGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGgc < 6:51053/149‑1 (MQ=255) gTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGgcg > 3:41249/1‑148 (MQ=255) tgcgtgGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCagag < 6:232414/149‑1 (MQ=255) cgtgGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCTCGGCGCattcac < 7:191413/149‑6 (MQ=255) gtgGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCTGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCaa < 5:90421/149‑1 (MQ=255) tgGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAg < 1:281461/149‑1 (MQ=255) tgGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAg < 5:53731/149‑1 (MQ=255) gggCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAgg < 2:56754/149‑1 (MQ=255) agcaCCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACAt > 1:134159/1‑149 (MQ=255) ttACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCa > 3:145575/1‑149 (MQ=255) ggACAGTGTCGCCATGGCGCTGCGCCTGGAAGATCGCTACAATGGCCTGCGCAGCCCCCACAAGCTCAAGGTCGCGGTATCGGGATGCACCGGTGAATGCGCCGAGGAGCAGAGCAAGGACATCGGCGTGATAGCCACGGCCAAGGGCt > 6:78661/1‑149 (MQ=255) ggACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCt < 3:113374/149‑1 (MQ=255) gACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGAGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTg > 6:11771/1‑149 (MQ=255) aCAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTgg < 7:233083/149‑1 (MQ=255) cGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGACACCGCCACGGGCTGGA‑ACCTGt > 4:205282/1‑149 (MQ=255) ccATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑Ac < 2:134159/149‑1 (MQ=255) cATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACa < 6:297199/131‑1 (MQ=255) cATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACa > 5:297199/1‑131 (MQ=255) gCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGtgtg < 4:145575/149‑1 (MQ=255) ggAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAAcggcg > 7:100604/1‑149 (MQ=255) ggAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAAcggcg > 8:59795/1‑149 (MQ=255) tCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑Ac > 1:109889/1‑121 (MQ=255) tCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑Ac < 2:109889/121‑1 (MQ=255) tCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATgc > 2:116850/1‑149 (MQ=255) cGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACAt < 7:110107/89‑1 (MQ=255) cGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACAt > 8:110107/1‑89 (MQ=255) cAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCcgc < 5:212103/149‑1 (MQ=255) aGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCgg < 7:59795/149‑1 (MQ=255) gggCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGATATCGCCACCGCCAAGGGCTGGA‑ACCTCT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCgg > 6:294029/1‑148 (MQ=255) ggCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCa > 2:49317/1‑149 (MQ=255) gCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCAc > 3:101543/1‑94 (MQ=255) gCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCAc < 4:101543/94‑1 (MQ=255) ccTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGTCACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCAt < 1:49317/149‑1 (MQ=255) gcAGCCCCCACCAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGCGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAg < 5:232797/149‑1 (MQ=255) gcAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAg < 8:57045/149‑1 (MQ=255) cacaAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGc > 2:85812/1‑149 (MQ=255) aaGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCAc < 8:100604/149‑1 (MQ=255) tCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGAc > 2:243787/1‑149 (MQ=255) aaGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCt > 7:135169/1‑149 (MQ=255) gTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGc < 6:243430/122‑1 (MQ=255) gTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGc > 5:243430/1‑122 (MQ=255) tCGCGGTATCGGTATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCTCCGACAAGGGCTGGA‑ACCTGT‑TCGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGga < 5:11771/148‑1 (MQ=255) cgcgGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGgac < 2:182819/148‑1 (MQ=255) cgcgGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGgac < 2:187874/148‑1 (MQ=255) ggTATCGGGATGCGCCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGgacgac > 3:216886/1‑148 (MQ=255) aTCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAg > 8:35494/1‑149 (MQ=255) ggATGGAGCCGTGAATGCGCCGTGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACgt < 5:284525/80‑1 (MQ=255) ggATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCt < 3:205282/149‑1 (MQ=255) gATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCTg > 7:218991/1‑149 (MQ=255) aCCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCTGGTACg > 8:22601/1‑149 (MQ=255) cccGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCTGGTACGc < 1:243787/149‑1 (MQ=255) ccGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCTGGTACGcc > 2:253180/1‑149 (MQ=255) gTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCTGGTACGCCt > 5:202438/1‑148 (MQ=255) ctctGCGCCTCGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGACACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGc < 1:64298/99‑1 (MQ=255) ctctGCGCCTCGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGc > 2:64298/4‑101 (MQ=255) tctGCGCCTCGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCTGGTACGCCTGATc > 4:228355/3‑149 (MQ=255) cGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCTGGTACGCCTGATCGACcgcg > 6:13542/1‑149 (MQ=255) | AAGTCGTGCGTGGGCAGCACCTGGTGCCGTTACGGCGTGCAGGACAGTGTCGCCATGGCCCTGCGCCTGGAAGATCGCTACAAGGGCCTGCGCAGCCCCCACAAGCTCAAGTTCGCGGTATCGGGATGCACCCGTGAATGCGCCGAGGCGCAGAGCAAGGACATCGGCGTTATCGCCACCGACAAGGGCTGGA‑ACCTGT‑ACGTGTGCGGTAACGGCGGCATGCGCCCGCGGCATGCCGAGCTGTTCGCCACCGACCTGGACGACGAAGCCCTGGTACGCCTGATCGACCGCG > NC_002947/1905675‑1905966 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |