Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,969,232 C→T 8.6% V96V (GTG→GTA)  ubiG ← ubiquinone biosynthesis O‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,969,2320CT 8.6% 126.8 / 2.4 47V96V (GTG→GTA) ubiGubiquinone biosynthesis O‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (24/18);  new base T (2/2);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

CTGGCCGCCCGGCTTGACCATGCGGTAGCAGGCGCGAATCACCGAAGACGGGTCGGGCACGTGCTCGAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGCCATGGCTTCGCTGAGGATGCCGCCACCGCAACCCACGTCCAGCACTTTCTT  >  NC_002947/1969085‑1969375
                                                                                                                                                   |                                                                                                                                                
cTGGCCGCCCGGCTTGACCATGCGGTAGCAGGCGCGAATCACCGAAGACGCGTCGGGCACGTGCTCGAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCa                                                                                                                                                 >  5:274116/1‑149 (MQ=255)
           gCTTGACCATGCGGTAGCAGGCGCGAATCACCGAAGACGGGTCGGGCACGTGCTCGAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCg                                                                                                                                      <  5:243181/149‑1 (MQ=255)
              tGACCATGCGGTAGCAGGCGCGAATCACCGAAGACGGGTCGGGCACGTGCTCGAGCATTTCCAGGCAGGTGACCCCGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGAc                                                                                                                                   >  8:136137/1‑149 (MQ=255)
                        gTAGCAGGCGCGAATCACCGAAGACGGGTCGGGCACGTGCTCGAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGGGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGt                                                                                                                         <  2:196474/149‑1 (MQ=255)
                             aGGCGCGAATCACCGAAGACGGGTCGGGCACGTGCTCGAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAAc                                                                                                                    <  5:213010/149‑1 (MQ=255)
                              ggCGCGAATCACCGAAGACGGGTCGGGCACGTGCTCGAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACt                                                                                                                   <  1:4362/149‑1 (MQ=255)
                               gcgcGAATCACCGAAGACGGGTCGGGCACGTGCTCGAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACt                                                                                                                   >  8:4509/1‑148 (MQ=255)
                               gcgcGAATCACCGAAGACGGGTCGGGCACGTGCTCGAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACt                                                                                                                   <  4:217917/148‑1 (MQ=255)
                               gcgcGAATCACCGAAGACGGGTCGGGCACGTGCTCGAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACt                                                                                                                   >  3:217917/1‑148 (MQ=255)
                                cgcgAATCACCGAAGACGGGTCGGGCACGTGCTCGAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGa                                                                                                                 <  8:60009/149‑1 (MQ=255)
                                      tCACCGAAGACGGGTCGGGCACGTGCTCGAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCAcc                                                                                                           >  3:146833/1‑149 (MQ=255)
                                        aCCGAAGACGGGTCGGGCACGTGCTCGAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGc                                                                                                         >  2:220791/1‑149 (MQ=255)
                                          cGAAGACGGGTCGGGCACGTGCTCGAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTgg                                                                                                                          <  1:2476/130‑1 (MQ=255)
                                          cGAAGACGGGTCGGGCACGTGCTCGAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTgg                                                                                                                          >  2:2476/1‑130 (MQ=255)
                                          cGAAGACGGGTCGGGCACGTGCTCGAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGc                                                                                                         >  6:53128/1‑147 (MQ=255)
                                              gACGGGTCGGGCACGTGCTCGAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTTCACGCCGGACTCCAGCTGGTGCAACTGAGCCACAGCAAGTgg                                                                                                   >  4:18199/1‑149 (MQ=255)
                                                                tCGAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTACACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCTCCAGTGGCGCCTCGCCCATGTCgc                                                                                  >  6:245205/1‑147 (MQ=255)
                                                                  gAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTTGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATAc                                                                               >  7:244273/1‑149 (MQ=255)
                                                                  gAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTc                                                                                             >  2:313102/1‑135 (MQ=255)
                                                                  gAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTc                                                                                             <  1:313102/135‑1 (MQ=255)
                                                                   aGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATAcc                                                                              >  6:61544/1‑149 (MQ=255)
                                                                        ttCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGCCCAGCGCTTCGGCGGTGATCTGCCGGTATTCAACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCCCCAGAGGCGCCTCGCCCAAGTCGATACCGCTGa                                                                         >  5:140724/1‑149 (MQ=255)
                                                                          ccAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCg                                                                                            <  7:150594/128‑1 (MQ=255)
                                                                          ccAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCg                                                                                            >  8:150594/1‑128 (MQ=255)
                                                                           caggcaggTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑cc                                                                      <  7:4509/148‑1 (MQ=255)
                                                                           caggcaggTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑cc                                                                      <  5:61544/148‑1 (MQ=255)
                                                                              gcaggTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTgg                                                                  >  1:179543/1‑149 (MQ=255)
                                                                                              aaCTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGCCAt                                                  >  2:246392/1‑149 (MQ=255)
                                                                                                      aGGCATTTCCTTGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGCCATGGCTTCGc                                          <  8:244273/149‑1 (MQ=255)
                                                                                                      aGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGCCATGGCTTCGc                                          >  7:93464/1‑149 (MQ=255)
                                                                                                         cATTTCCTCGGCCAGGGCTTCGGCGGTGATCGGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGCCATGGCTTCGCTGa                                       <  7:136137/149‑1 (MQ=255)
                                                                                                         cATTTCCTCGGCCAGGGCTTCCGCGGTGATCTGCCGGTATTCCACCTGCTCGCCGGACTCCAGCTGGTGCAACTGAGGCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGCCATGGCTTCGCTGa                                       <  6:274116/149‑1 (MQ=255)
                                                                                                               cTCGGCCAGGGCTTCGGCGGTTATCTGCCGGTATTCCAACTTCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGGCATTGGCGGCTCGCCCATGTCGTTACCGGTGACCCGTGGCGCCGCGCAGGGCCATGGCTTCGCTGAGGAt                                   <  1:220791/148‑1 (MQ=255)
                                                                                                               cTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGCCATGGCTTCGCTGAGGATgc                                 >  6:314605/1‑149 (MQ=255)
                                                                                                                     cAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGCCATGGCTTCGCTGAGGATGCCGCCAc                           >  3:300398/1‑149 (MQ=255)
                                                                                                                     cAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGCCATGGCTTCGCTGAGGATGCCGCCAc                           >  2:100305/1‑149 (MQ=255)
                                                                                                                     cAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGCCATGGCTTCGCTGAGGATGCCGCCAc                           >  8:54549/1‑149 (MQ=255)
                                                                                                                        ggCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGCCATGGCTTCGCTGAGGATGCCGCCACCg                         >  6:304039/1‑148 (MQ=255)
                                                                                                                         gCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGCCATGGc                                               <  2:323280/125‑1 (MQ=255)
                                                                                                                         gCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGCCATGGc                                               >  1:323280/1‑125 (MQ=255)
                                                                                                                           ttCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGTCATGGCTTCGCTGAGGATGCCGCCACCGCAAc                     >  6:76652/1‑149 (MQ=255)
                                                                                                                                     aTCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGCCATGGCTTCGCTGAGGATGCCGCCACCGCAACCCACGTCCa            <  8:93464/148‑1 (MQ=255)
                                                                                                                                          ccGGTATTCTACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGCCATGGCTTCGCTGAGGATGCCGCCACCGCAACCCACGt               >  7:231133/1‑140 (MQ=255)
                                                                                                                                          ccGGTATTCTACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGCCATGGCTTCGCTGAGGATGCCGCCACCGCAACCCACGt               <  2:270303/140‑1 (MQ=255)
                                                                                                                                          ccGGTATTCTACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGCCATGGCTTCGCTGAGGATGCCGCCACCGCAACCCACGt               <  8:231133/140‑1 (MQ=255)
                                                                                                                                          ccGGTATTCTACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGCCATGGCTTCGCTGAGGATGCCGCCACCGCAACCCACGt               >  1:270303/1‑140 (MQ=255)
                                                                                                                                              tATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGCCATGGCTTCGCTGAGGATGCCGCCACCGCAACCCACGTCCAGCACTTTCtt  <  1:246392/149‑1 (MQ=255)
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CTGGCCGCCCGGCTTGACCATGCGGTAGCAGGCGCGAATCACCGAAGACGGGTCGGGCACGTGCTCGAGCATTTCCAGGCAGGTGACCACGTCGAACTGTTCAGGCATTTCCTCGGCCAGGGCTTCGGCGGTGATCTGCCGGTATTCCACCTGCACGCCGGACTCCAGCTGGTGCAACTGAGCCACCGCCAGTGGCGCCTCGCCCATGTCGATACCGGTGA‑CCGTGGCGCCGCGCAGGGCCATGGCTTCGCTGAGGATGCCGCCACCGCAACCCACGTCCAGCACTTTCTT  >  NC_002947/1969085‑1969375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑