Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,980,991 A→G 6.2% N554S (AAC→AGC)  rpsA → 30S ribosomal protein S1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,980,9910AG 6.2% 186.2 / 2.4 66N554S (AAC→AGC) rpsA30S ribosomal protein S1
Reads supporting (aligned to +/- strand):  ref base A (32/28);  new base G (2/2);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.48e-01

CGCGTTGAAGACGCGCGTAACGTTCTGAAGGAAGGCGAAGAGATCGAAGCCAAGATCATCAGCGTTGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGAT  >  NC_002947/1980843‑1981137
                                                                                                                                                    |                                                                                                                                                  
cgcgTTGACGACGCGCGTAACGTTCTGAAGGAAGGCGAAGAGATCGAAGCCAAGATCATCAGCGTTGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGaaaaa                                                                                                                                                    <  2:176484/149‑1 (MQ=255)
 gcgTTGAAGACGCGCGTAACGTTCTGAAGGAAGGCGAAGAGATCGAAGCCAAGATCATCAGCGTTGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAAc                                                                                                                                                   <  2:213033/149‑1 (MQ=255)
      gAAGACGCGCGTAACGTTCTGAAGGAAGGCGAAGAGATCGAAGCCAAGATCATCAGCGTTGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAAccatcc                                                                                                                                              >  4:37363/1‑144 (MQ=255)
       cttcatcgcgTAACGTTCTGAAGGAAGGCGAAGAGATCGAAGCCAAGATCATCAGCGTTGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTc                                                                                                                                               <  6:162651/141‑1 (MQ=255)
                gTAACGTTCTGAAGGAAGGCGAAGAGATCGAAGCCAAGATCATCAGCGTTGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAgctgct                                                                                                                                    >  5:226013/1‑149 (MQ=255)
                     gTTCTGAAGGAAGGCGAAGAGATCGAAGCCAAGATCATCAGCGTAGACCGCAAGTCCCGCGATATCAGCCTCTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCAGAAAAACGCTCCGGAAGCTGCAGCCGa                                                                                                                               >  6:1273/1‑149 (MQ=255)
                            aggaaggCGAAGAGATCGAAGCCAAGATCATCAGCGTTGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACaccacc                                                                                                                        <  4:119217/149‑1 (MQ=255)
                            aggaaggCGAAGAGATCGAAGCCAAGATCATCAGCGTTGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACaccacc                                                                                                                        <  8:274620/149‑1 (MQ=255)
                              gaaggCGAAGAGATCGAAGCCAAGATCATCAGCGTTGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCAGGAAGCTGCTGCCGACACCACCAt                                                                                                                      >  3:253434/1‑149 (MQ=255)
                                  gccaaGCGAGCGAAGCCAAGATCATCAGCGTTGACCGCAAGTCCCGCGTTATCCGCCTGTCGATCAAATCGAACGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGgct                                                                                                                  <  8:229571/146‑1 (MQ=255)
                                    gAAGAGATCGAAGCCAAGATCATCAGCGTTGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAg                                                                                                                                         >  6:261592/1‑124 (MQ=255)
                                    gAAGAGATCGAAGCCAAGATCATCAGCGTTGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAg                                                                                                                                         <  5:261592/124‑1 (MQ=255)
                                             gAAGCCAAGATCATCAGCGTTGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAATCGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTgcg                                                                                                       <  5:1273/149‑1 (MQ=255)
                                                 ccAAGATCATCAGCGTTGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGaa                                                                                                   <  6:226013/149‑1 (MQ=255)
                                                  cAAGATCATCAGCGTTGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAg                                                                                                  <  8:40733/149‑1 (MQ=255)
                                                  cAAGATCATCAGCGTTGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAg                                                                                                  >  3:314376/1‑149 (MQ=255)
                                                   aaGATCATCAGCGTTGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAg                                                                                                  >  1:20577/1‑148 (MQ=255)
                                                   aaGATCATCAGCGTTGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAg                                                                                                  >  1:119641/1‑148 (MQ=255)
                                                   aaGATCATCAGCGTTGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGc                                                                                                 <  4:314376/149‑1 (MQ=255)
                                                       tcatcaGCGTTGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATg                                                                                             <  1:158116/149‑1 (MQ=255)
                                                         atcaGCGTTGACCGCAAGTCCCGCGTTATCAGTCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATggt                                                                                           <  7:9087/149‑2 (MQ=255)
                                                         atcaGCGTCGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGc                                                                                           >  4:129448/1‑149 (MQ=255)
                                                            accGTTGACCCCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAACAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGTCCa                                                                                         <  3:37363/146‑1 (MQ=255)
                                                                     cGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACt                                                                                >  7:267897/1‑148 (MQ=255)
                                                                     cGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAACAGAACt                                                                                >  8:252112/1‑148 (MQ=255)
                                                                       cAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAg                                                                             >  5:32657/1‑149 (MQ=255)
                                                                       cAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAg                                                                             >  2:76221/1‑149 (MQ=255)
                                                                        aaGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGt                                                                            >  3:191879/1‑149 (MQ=255)
                                                                             ccGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGt                                                                       <  4:253434/149‑1 (MQ=255)
                                                                              cgcgTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGtt                                                                      <  4:191879/149‑1 (MQ=255)
                                                                              cgcgTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGtt                                                                      >  3:25713/1‑149 (MQ=255)
                                                                               gcgTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAGCGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGttt                                                                     >  5:307096/1‑149 (MQ=255)
                                                                               gcgTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAGCGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGttt                                                                     >  5:302226/1‑149 (MQ=255)
                                                                                 gTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAACAGAACTCAGTTCTGCTTgg                                                                   <  7:252112/149‑2 (MQ=255)
                                                                                    aTCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATc                                                                 <  5:212564/148‑1 (MQ=255)
                                                                                     tCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCgg                                                               >  2:303043/1‑149 (MQ=255)
                                                                                     tCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCgg                                                               >  1:264411/1‑149 (MQ=255)
                                                                                      cAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCgg                                                               <  8:267897/148‑1 (MQ=255)
                                                                                        gCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTa                                                             >  5:34209/1‑148 (MQ=255)
                                                                                        gCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTa                                                             >  2:162677/1‑148 (MQ=255)
                                                                                           tGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGt                                                                       >  4:25985/1‑135 (MQ=255)
                                                                                           tGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGt                                                                       <  3:25985/135‑1 (MQ=255)
                                                                                           tGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTaaa                                                           >  5:269971/1‑147 (MQ=255)
                                                                                             tCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAgg                                                       >  2:77519/1‑149 (MQ=255)
                                                                                                aTCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCaa                                                                                        >  8:36497/1‑113 (MQ=255)
                                                                                                 tCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAACCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGa                                                   >  1:229087/1‑149 (MQ=255)
                                                                                                      tCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCtgctgc                                                                                                        <  6:183635/91‑1 (MQ=255)
                                                                                                      tCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCtgctgc                                                                                                        >  5:183635/1‑91 (MQ=255)
                                                                                                        gAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCgg                                            <  4:25713/149‑1 (MQ=255)
                                                                                                         aaaGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAg                                                                             <  8:74531/115‑1 (MQ=255)
                                                                                                         aaaGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAg                                                                             >  7:74531/1‑115 (MQ=255)
                                                                                                          aaGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCggg                                           <  6:34209/148‑1 (MQ=255)
                                                                                                             acgacgCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGc                                       <  1:76221/149‑1 (MQ=255)
                                                                                                                 cgCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAGCGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCtt                                   <  6:307096/149‑1 (MQ=255)
                                                                                                                 cgCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAGCGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCtt                                   <  6:302226/149‑1 (MQ=255)
                                                                                                                 cgCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCtt                                   <  6:269971/149‑1 (MQ=255)
                                                                                                                  gCTGAAGAGCGCGAAGCTATCCAGAGCCTGAATAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCttt                                  <  2:21040/149‑1 (MQ=255)
                                                                                                                    tGAAGCCCGCGAAGCTATCCAGAGCCTGAAAACCGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCCCGGGTCGCCCt                                    <  8:118391/145‑1 (MQ=255)
                                                                                                                    tGAAGAGCGCGAAGCTATCCAGAGCCTGAGAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCa                                                                                     >  6:40230/1‑96 (MQ=255)
                                                                                                                    tGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCCCGGGTCGCCCt                                    >  7:118391/1‑145 (MQ=255)
                                                                                                                      aaGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCttttttt                              <  1:162677/149‑1 (MQ=255)
                                                                                                                      aaGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCtttttt                               <  2:119641/148‑1 (MQ=255)
                                                                                                                              gAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTc                                              >  5:2807/1‑125 (MQ=255)
                                                                                                                              gAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTc                                              <  6:2807/125‑1 (MQ=255)
                                                                                                                                    aTCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACAACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAg                >  7:235327/1‑149 (MQ=255)
                                                                                                                                     tCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAgg               >  5:319117/1‑149 (MQ=255)
                                                                                                                                        agagCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAggtgg            >  7:296113/1‑149 (MQ=255)
                                                                                                                                                aaaaaCGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTgcggc      <  3:129448/147‑1 (MQ=255)
                                                                                                                                                aaaaaCGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTgcggc      <  1:77519/147‑1 (MQ=255)
                                                                                                                                                aaaaaCGCTCCGGAAGCTACTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTgcggcg     >  8:308556/1‑148 (MQ=255)
                                                                                                                                                  aaaCGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGAt  <  2:20577/149‑1 (MQ=255)
                                                                                                                                                  aaaCGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGAt  <  8:296113/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGCGTTGAAGACGCGCGTAACGTTCTGAAGGAAGGCGAAGAGATCGAAGCCAAGATCATCAGCGTTGACCGCAAGTCCCGCGTTATCAGCCTGTCGATCAAATCGAAAGACGACGCTGAAGAGCGCGAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGAT  >  NC_002947/1980843‑1981137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑