Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	213,354	T→A	9.6%	L6287Q (CTG→CAG)	PP_0168 →	putative surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	213,354	0	T	A	9.6%	185.2 / 8.1	70	L6287Q (CTG→CAG)	PP_0168	putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base T (34/29);  new base A (4/3);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

CACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGCCACT  >  NC_002947/213211‑213499                                                                                                                                                |                                                                                                                                                   cACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTc                                                                                                                                                <  1:12409/148‑1 (MQ=255) cACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTc                                                                                                                                                >  2:12409/1‑148 (MQ=255)  aCGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGTCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGt                                                                                                                                                 <  3:269741/146‑1 (MQ=255)  aCGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCAGGTc                                                                                                                                                >  2:224213/1‑147 (MQ=255)  aCGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGAACTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGt                                                                                                                                                 >  4:269741/1‑146 (MQ=255)           aCCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGa                                                                                                                                      <  1:231875/148‑1 (MQ=255)           aCCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGa                                                                                                                                      <  5:270016/148‑1 (MQ=255)                           ccccAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtc                                                                                                                     <  4:82644/149‑1 (MQ=255)                             ccAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcat                                                                                                                   >  7:191947/1‑149 (MQ=255)                              cAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcatc                                                                                                                  <  2:265475/149‑1 (MQ=255)                              cAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcatc                                                                                                                  <  5:61566/149‑1 (MQ=255)                                 gCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcatcat                                                                                                                >  2:48783/1‑148 (MQ=255)                                   aaCGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcatcatc                                                                                                               >  1:44033/1‑147 (MQ=255)                                   aaCGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGtcatcatc                                                                                                               >  4:252396/1‑147 (MQ=255)                                   aaCGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCaa                                                                                                             <  1:224213/149‑1 (MQ=255)                                    aCGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAg                                                                                                            >  3:272403/1‑149 (MQ=255)                                    aCGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAg                                                                                                            <  5:114841/149‑1 (MQ=255)                                     cGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAgg                                                                                                           <  8:191947/149‑1 (MQ=255)                                        aGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATTACCAGTTCATAATCAACGCTg                                                                                                        >  6:229355/1‑149 (MQ=18)                                                   tCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTc                                                                                                                                                <  1:299264/98‑1 (MQ=255)                                                   tCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTc                                                                                                                                                >  2:299264/1‑98 (MQ=255)                                                    cAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAAc                                                                                                 >  5:15249/1‑144 (MQ=255)                                                    cAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAAc                                                                                                 <  6:15249/144‑1 (MQ=255)                                                     aaGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGc                                                                                                      <  1:303838/138‑1 (MQ=255)                                                     aaGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGc                                                                                                      >  2:303838/1‑138 (MQ=255)                                                      aGGTCACCATCACGGCCCACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAg‑cgcg                                                                                         >  5:90883/1‑149 (MQ=255)                                                        gTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAg‑cgcgc                                                                                        >  1:157267/1‑148 (MQ=255)                                                           aCCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATa                                                                                    >  7:11345/1‑149 (MQ=255)                                                           aCCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCAAGGTCATCATCCAGGCTGGCGAAACCAG‑CGCGCCAt                                                                                     <  1:48783/148‑1 (MQ=255)                                                               tCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACAcc                                                                                >  4:132960/1‑149 (MQ=255)                                                                  cGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCAc                                                                             >  4:103174/1‑149 (MQ=255)                                                                   ggCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACg                                                                            <  2:157267/149‑1 (MQ=255)                                                                    gCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGc                                                                           >  3:259432/1‑149 (MQ=255)                                                                            gACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAg                                                                   >  7:121213/1‑149 (MQ=255)                                                                               cTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCg                                                                <  6:90883/149‑1 (MQ=255)                                                                                tCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGa                                                               >  1:233589/1‑149 (MQ=255)                                                                                 cGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAgg                                                                  >  2:168031/1‑145 (MQ=255)                                                                                 cGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAgg                                                                  <  1:168031/145‑1 (MQ=255)                                                                                  ggTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGa                                                               <  2:44033/147‑1 (MQ=255)                                                                                   gTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGa                                                            <  4:272403/149‑1 (MQ=255)                                                                                               gTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGa                                                <  1:162086/149‑1 (MQ=255)                                                                                                tCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGAt                                               <  8:11345/149‑1 (MQ=255)                                                                                                  aaGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATg                                              <  2:233589/148‑1 (MQ=255)                                                                                                   aGCCGACGTACACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCt                                            >  6:151997/1‑149 (MQ=255)                                                                                                      cGACATTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGc                                         >  6:144601/1‑149 (MQ=255)                                                                                                                  tGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTg                             <  8:121213/149‑1 (MQ=255)                                                                                                                    cacaTCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTggg                           <  4:259432/149‑1 (MQ=255)                                                                                                                      caTCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCa                         <  4:33665/149‑1 (MQ=255)                                                                                                                       aTCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATacac                                                                                 <  1:203737/92‑1 (MQ=255)                                                                                                                       aTCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATacac                                                                                 >  2:203737/1‑92 (MQ=255)                                                                                                                       aTCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCAt                        >  8:95325/1‑149 (MQ=255)                                                                                                                       aTCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCAt                        >  5:130026/1‑149 (MQ=255)                                                                                                                         cAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCAtt                       >  5:319994/1‑148 (MQ=255)                                                                                                                           acacCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAAt                    >  8:105967/1‑149 (MQ=255)                                                                                                                             acCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTc                  >  8:37063/1‑149 (MQ=255)                                                                                                                               cGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCgg                >  6:2631/1‑149 (MQ=255)                                                                                                                               cGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCgg                <  6:319994/149‑1 (MQ=255)                                                                                                                                 ccTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCgg                >  2:21773/1‑147 (MQ=255)                                                                                                                                  tcagggtCACGACCAGGTCGTGAGCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAAt                    >  3:182215/7‑142 (MQ=255)                                                                                                                                  tcagggtCACGACCAGGTCGTGAGCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAAt                    <  4:182215/136‑1 (MQ=255)                                                                                                                                  cTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACCCCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAg             >  8:50947/1‑149 (MQ=255)                                                                                                                                  cTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCCCGCTGCGCAAGGCGCTGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCgg                >  4:273624/1‑146 (MQ=255)                                                                                                                                    agggtCACGACCAGGTCGTGAGCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCgg                <  2:40808/140‑1 (MQ=255)                                                                                                                                    agggtCACGACCAGGTCGTGAGCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCgg                >  1:40808/5‑144 (MQ=255)                                                                                                                                        tCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACg       >  1:241117/1‑149 (MQ=255)                                                                                                                                         cACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGc      <  3:103174/149‑1 (MQ=255)                                                                                                                                          aCGACCTGGTCGTGACCCTGAGCAACAATGCACAGGTAATCATCAAGGCTGG‑ATAACAAGCCACGCCATACCCCCACAATCCGAAAGACGATAACGTCTACAACGATGCTGGCCAGATCAGCCGGGGCATTAATTCGGCAGTGGACGc      >  6:259620/1‑148 (MQ=14)                                                                                                                                          aCGACCAGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGa         >  2:51655/1‑145 (MQ=255)                                                                                                                                          aCGACCAGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGa         <  1:51655/145‑1 (MQ=255)                                                                                                                                             aCCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACTCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGTCACt  <  5:229355/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                   CACGTCGGTGACCGACGAGCCGGGTACCCCAGGCAACGAAGGCGACTTGGTCAAGGTCACCATCACGGCCGACCAGACCTCGGTGGCCGAGAACGTCAAGCCGACGTTCACCGTGCACATCAACACCGCCTTGGCTCACGACCTGGTCGTGACCCTGAGCAACAATGCCCAGGTCATCATCAAGGCTGGCGAAACCAG‑CGCGCCATACACCCACGCTGCGCAAGGCGATGACGTCTACAACGATGCTGGCCAGATCAGCCTGGGCATTAATTCGGCAGTGGACGCCACT  >  NC_002947/213211‑213499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑