Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,073,373 A→C 13.1% L12R (CTG→CGG)  PP_1847 ← TonB‑dependent ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,073,3730AC13.1% 76.5 / 4.1 37L12R (CTG→CGG) PP_1847TonB‑dependent ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base A (16/12);  new base C (2/3);  total (18/19)
Fisher's exact test for biased strand distribution p-value = 6.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGCTGGAAGCCGCCCACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATACACCAGATGGTGATCCCCCTGTAGGAGCGGGTTTAC  >  NC_002947/2073233‑2073520
                                                                                                                                            |                                                                                                                                                   
cgGGCTGGAAGCCGCCCACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAgg                                                                                                                                             <  4:136336/149‑1 (MQ=255)
 gggCTGGAAGCCGCCCACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAggg                                                                                                                                            <  8:64328/149‑1 (MQ=255)
 gggCTGGAAGCCGCCCACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGACGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGTGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAggg                                                                                                                                            <  8:149007/149‑1 (MQ=255)
    cTGGAAGCCGCCCACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCCTGCTCAgcctgg                                                                                                                                         <  8:240318/149‑6 (MQ=255)
      ggAAGCCGCCCACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAgg                                                                                                                                       <  3:260653/149‑1 (MQ=255)
              cccACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCTCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTg                                                                                                                               <  2:223177/149‑1 (MQ=255)
              cccACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGt                                                                                                                                >  3:295574/1‑148 (MQ=255)
                            gCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCCGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGc                                                                                                                 >  6:181443/1‑149 (MQ=255)
                                gccggttAGCCATCATTGGCGTAGGTGTCGGACACCTGCAATGGTGCCAGCATCACGCTGTCTTCGGCAACCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTg                                                                                                             <  2:43731/143‑1 (MQ=255)
                                 ccTGGTAGCCAGCATTGGCGTAGGGGGCGGAGACCTGCAAGGGGGCCAGCATCACGCTGTCTTGGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTgg                                                                                                            <  3:254291/149‑1 (MQ=255)
                                                  gCGAAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACAGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGCCGGGGGTGATGCGGGCGCATGCGGTGGGGCTCCTCGCTGCGCGa                                                                                           >  3:241043/1‑149 (MQ=255)
                                                             ggAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGGTGCGCGATACCTGTACTg                                                                                >  2:141042/1‑149 (MQ=255)
                                                                   cTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCtt                                                                          >  5:73971/1‑149 (MQ=255)
                                                                   cTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCGGTACTGGCCCtt                                                                          >  3:219034/1‑149 (MQ=255)
                                                                   cTGCAATGGGGCCACCATCACGCTGTCTTCGGCAAGCACAGAGGTGCACTGCAGGGCCAGGCCCAGGCAGAGCAGCCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCTCTGGGGCTCCTGGCAGCGCGATACCTGTACTTGCCCtt                                                                          >  5:330835/1‑149 (MQ=255)
                                                                     gCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTc                                                                        >  1:244180/1‑149 (MQ=255)
                                                                          ggggCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCggg                                                                   >  7:155511/1‑149 (MQ=255)
                                                                                              ttCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACCCCgg                                               >  7:189939/1‑149 (MQ=255)
                                                                                                 ggCAACCACACTGGGGCACTGCCGGGCCAGGCCCAGGCTGTGCTGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGggtg                                            <  8:182561/149‑1 (MQ=255)
                                                                                                  gCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCggg                                                                   >  5:246080/1‑125 (MQ=255)
                                                                                                   cAAGCACAGCGGGGCAGTGCAGGGCCTGGCCAAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGAGGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGggtggt                                          <  1:132405/149‑1 (MQ=255)
                                                                                                   cAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCACGCGGGGGTGATGCGGGGGCATGCGGTGCGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGTGTAAACCCACTCCTACACCGggtggt                                          >  5:137983/1‑149 (MQ=255)
                                                                                                      gCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGa                                        >  3:296064/1‑148 (MQ=255)
                                                                                                          agcggggCACGGCAGGGCCCGGCCCTGGCTGAGCTGGCTCAGGGCCCGGCGGGGGTGAGGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATacac                                   <  8:155511/146‑1 (MQ=255)
                                                                                                           gagGGGCCCGCCCGGGCCAGGCCCCGGCGCCCCCGCCCCAGGGCCCGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATACAcc                                  <  1:191129/149‑1 (MQ=255)
                                                                                                                 cACTGCTGGGCCTGGCCCAGGCTGAGCTGGCTCAGGGGCTGGGGGGGGTGTTGGGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATACACCAGATgg                            <  2:244180/149‑1 (MQ=255)
                                                                                                                  acggCAGGGCCCGGCCCTGGCTTCGCAGGCTCCGTGCCAGGGGGGGGTTATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATACACCAGATGGt                           <  1:130993/146‑1 (MQ=255)
                                                                                                                            caggcccaggcTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCgggg                                                                                                                       <  5:31704/47‑1 (MQ=255)
                                                                                                                            caggcccaggcTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCgggg                                                                                                                       >  6:31704/1‑47 (MQ=255)
                                                                                                                            caggcccaggcTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATACACCAGATGGTGATCCCCCTg                 >  6:131030/1‑149 (MQ=255)
                                                                                                                             aggcccaggcTGAGCCGGCTCAGGGGCCGGCGGGGGTGTTGCGGGGCCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATACACCAGATGGTGATCCCCCTGt                <  4:296064/149‑1 (MQ=255)
                                                                                                                              ggcccaggcTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGTGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATACACCAGATGGTGATCCCCCTGTa               <  4:295574/149‑1 (MQ=255)
                                                                                                                               gcccaggcTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGGTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATACACCAGATGGTGa                         >  1:213224/1‑138 (MQ=255)
                                                                                                                                  gccccTGAGCCTGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATACACCAGATGGTGATCCCCCTGTAGGAg           >  1:214329/5‑149 (MQ=255)
                                                                                                                                    cccTGAGCCTGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGCTACCTGTACTGGCCCTTTCGCGGGTAAACCCACACCTACACCGGGGGGTGATACACCAGCTGGTGATCCCCCTGTAGGcgcg         >  8:38879/3‑149 (MQ=255)
                                                                                                                                     gcTGTGCTGGGTCTGGGGCTGGCGGGGGTCTTGCGGGGGCATGGGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATACACCAGATGGTGATCCCCCTGTAGGAGCgg        <  1:302554/149‑1 (MQ=255)
                                                                                                                                           cAGGATCAGGGGCAGGCGGGGGTCAGGCGGGGGCAGGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCTGGTAAACCCACTCCTACACCGGGTGGTGATACACCAGATGGTGATCCCCCTGTAGGAGCGGGTTTAc  <  5:181443/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
CGGGCTGGAAGCCGCCCACAGCGGCCTGGCGTGCCTGGTAGCCATCATTGGCGTAGGTGTCGGAGACCTGCAATGGGGCCAGCATCACGCTGTCTTCGGCAAGCACAGAGGGGCACTGCAGGGCCAGGCCCAGGCTGAGCAGGCTCAGGGGCAGGCGGGGGTGATGCGGGGGCATGCGGTGGGGCTCCTGGATGCGCGATACCTGTACTGGCCCTTTCGCGGGTAAACCCACTCCTACACCGGGTGGTGATACACCAGATGGTGATCCCCCTGTAGGAGCGGGTTTAC  >  NC_002947/2073233‑2073520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑