Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,102,999 C→G 11.9% noncoding (35/76 nt) PP_t23 → tRNA‑Gly

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,102,9990CG11.9% 129.8 / 8.8 50noncoding (35/76 nt)PP_t23tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base C (17/27);  new base G (3/3);  total (20/30)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

GGTTCATGTTCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAG  >  NC_002947/2102852‑2103140
                                                                                                                                                   |                                                                                                                                             
ggTACATGTTCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGc                                                                                                                                               >  6:165708/1‑148 (MQ=255)
 gTTCATGTTCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCACTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGcc                                                                                                                                              >  7:55140/1‑148 (MQ=255)
        ttCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGACCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGGCAAGGTCgt                                                                                                                                      <  5:245339/149‑2 (MQ=255)
          cTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCggg                                                                                                                                     <  6:233488/148‑1 (MQ=255)
              tGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgc                                                                                                                                <  3:166507/149‑1 (MQ=255)
              tGCACTGAAAAGGGTTGTGTGTCCCCTTCGTCGAGTGGCCTAGGACACCGCCCTTTCCCGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTcgc                                                                                                                                <  5:139104/149‑1 (MQ=255)
                   tGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGtt                                                                                                                           >  7:150653/1‑149 (MQ=255)
                     aaaaGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTc                                                                                                                          >  7:148375/1‑148 (MQ=255)
                            ttgtGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGATCAGTTGGTAGAGCACGACCTTGCCAACGTCGGGGTCGCGAGTTCGAGTCTCg                                                                                                                  >  1:134060/1‑149 (MQ=255)
                              gtgtgtCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGa                                                                                                                              <  6:22040/135‑1 (MQ=37)
                              gtgagtCCCCTTCGTCTAGTGGCCTAGGACACCCCCCTTTCACGGCGGTAACAGCGGTTCGAGTACCCTAGGGGACGCCAAAAGCGCGAATAGCTCAGTTGCTAGAGCACGACCATGCCAAGGTCGGGGTCGCTAGATCGAGACTCGtt                                                                                                                >  8:299368/1‑149 (MQ=11)
                                       cTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTc                                                                                                        >  2:1545/1‑148 (MQ=34)
                                        ttCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTc                                                                                                        >  1:202334/1‑147 (MQ=34)
                                           gTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGa                                                                                                                        <  2:177715/128‑1 (MQ=35)
                                           gTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGtt                                                                                                   >  1:200408/1‑149 (MQ=34)
                                           gTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGtt                                                                                                   >  2:238059/1‑149 (MQ=34)
                                           gTCTAGTGGCCTAGGACACCGCCCTTGCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGa                                                                                                                        >  1:177715/1‑128 (MQ=25)
                                            tCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGtt                                                                                                   >  6:156154/1‑148 (MQ=34)
                                                gTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTaa                                                                                                <  8:148375/147‑1 (MQ=35)
                                                gTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTaa                                                                                                <  8:150653/147‑1 (MQ=35)
                                                        ggACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGttt                                                                                                  <  2:133291/137‑1 (MQ=34)
                                                        ggACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGttt                                                                                                  >  1:133291/1‑137 (MQ=34)
                                                                   tgaaagggcggTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGt                                                                           <  5:15568/143‑1 (MQ=255)
                                                                        cggcggTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCTAGTTTAAATACTGTGGCGTCTATGCGGTGCAgt                                                                      >  5:145121/1‑149 (MQ=255)
                                                                         ggcggTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAgtg                                                                     <  2:200408/149‑1 (MQ=255)
                                                                         ggcggTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAgtg                                                                     <  2:106520/149‑1 (MQ=255)
                                                                           cggTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAgtggt                                                                   <  2:202334/149‑1 (MQ=255)
                                                                                 taGGGGTTCGAGTCCCGTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGATTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGaa                                                             <  7:299368/148‑1 (MQ=255)
                                                                                 cAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAgtggtggtg                                                               >  1:128892/1‑147 (MQ=255)
                                                                                      gTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTc                                                                                                                                        >  8:186592/1‑69 (MQ=40)
                                                                                          gAGTCCGCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTaa                                                     <  8:78335/148‑1 (MQ=255)
                                                                                           aGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTaatg                                                   <  2:128892/149‑3 (MQ=255)
                                                                                            gTCCCCTAGGGGAAGCCAAATGCGGGAATAGCTCAGTTGTTAGAGCACTACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAgg                                                  <  8:55140/149‑1 (MQ=255)
                                                                                                     gggACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACg                                         >  1:23623/1‑149 (MQ=255)
                                                                                                             aaaTGCGGGAATAGCTCTGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGt                                 >  4:202262/1‑149 (MQ=255)
                                                                                                                tGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGt                              <  1:1545/149‑1 (MQ=255)
                                                                                                                tGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCAGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGt                              <  1:238059/149‑1 (MQ=255)
                                                                                                                    ggAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAg                          <  5:156154/149‑1 (MQ=255)
                                                                                                                       aTAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGGGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCtt                       <  8:212912/149‑1 (MQ=255)
                                                                                                                        tAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCTAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTc                      <  6:145121/149‑1 (MQ=255)
                                                                                                                           cTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGGGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCaaa                   <  5:165708/149‑1 (MQ=255)
                                                                                                                              aGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAAtt                 <  1:19188/148‑1 (MQ=255)
                                                                                                                                ttGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCg              >  3:196013/1‑149 (MQ=255)
                                                                                                                                  ggTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCgg             <  2:134060/148‑1 (MQ=255)
                                                                                                                                  ggTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCgg             <  3:202262/148‑1 (MQ=255)
                                                                                                                                         cccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGc      >  1:119050/3‑148 (MQ=255)
                                                                                                                                          ccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGgc                                                 >  4:264033/2‑104 (MQ=255)
                                                                                                                                          ccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGgc                                                 <  3:264033/103‑1 (MQ=255)
                                                                                                                                          ccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAAc                                   >  3:20035/2‑118 (MQ=255)
                                                                                                                                          ccGACCTTGGCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAAc                                   <  4:20035/117‑1 (MQ=255)
                                                                                                                                           cGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCa   <  2:23623/149‑1 (MQ=255)
                                                                                                                                            gACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAg  <  4:196013/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
GGTTCATGTTCTGGTGCACTGAAAAGTGTTGTGTGTCCCCTTCGTCTAGTGGCCTAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGACGCCAAATGCGGGAATAGCTCAGTTGGTAGAGCACGACCTTGCCAAGGTCGGGGTCGCGAGTTCGAGTCTCGTTTCCCGCTCCAGTTTAAATACTGTGGCGTCTATGCGGTGCAGTGGTGGTGAAGGTCGTAAAGGCGCTTCACGCCGAACGTGGTAAAGCTTCAAATTGCGGGAATAGCTCAG  >  NC_002947/2102852‑2103140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑