Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,171,167 C→T 9.5% T170M (ACG→ATG)  PP_5484 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,171,1670CT 9.5% 113.2 / 2.1 42T170M (ACG→ATG) PP_5484hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (17/21);  new base T (2/2);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGTGAGGCTACATGGCGTCGAGGGGGAGTCACTGACAAAGGCGCAGGAAGAGGTGATAGAGAGCAAGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCGGGTGACTTTCTGGTACCGGATAGCTTTGAAGACATGATGATCGTGCCGATCTCACCCCGTTTGGCGATCGTTGCCGGCGTGCCTGACTGC  >  NC_002947/2171025‑2171312
                                                                                                                                              |                                                                                                                                                 
gaTGTGAGGCTACATGGCGTCGAGGGGGAGTCACTGACAAAGGCGCAGGAAGAGGTGATAGAGAGCAAGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCtt                                                                                                                                             <  8:201297/149‑1 (MQ=255)
 aTGTGAGGCTACATGGCGTCGAGGGGGAGTCACTGACAAAGGCGCAGGAAGAGGTGATAGAGAGCAAGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTc                                                                                                                                            <  5:215554/149‑1 (MQ=255)
  tgtgAGGCTACATGGCGTCGAGGGGGAGTCACTGACAAAGGCGCAGGAAGAGGTGATAGAGAGCAAGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTc                                                                                                                                            <  7:85654/148‑1 (MQ=255)
    tgAGGCTACATGGCGTCGAGGGGGAGTCACTGACAAAGGCGCAGGAAGAGGTGATAGAGAGCAAGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATa                                                                                                                                         >  5:200815/1‑149 (MQ=255)
             aTGGCGTCGAGGGGGAGTCACTGACAAAGGCGGAGGAAGATGTTATATAGAGCAAGCACGAGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAAc                                                                                                                                <  6:113801/149‑1 (MQ=255)
             aTGGCGTCGAGGGGGAGTCACTGACAAAGGCGCAGGAAGAGGTGATAGAGAGCAAGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAAc                                                                                                                                <  2:51472/149‑1 (MQ=255)
                      aGGGGGAGTCACTGACAAAGGCGCAGGAAGAGGTGATAGAGAGCAAGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATg                                                                                                                       <  6:180105/149‑1 (MQ=255)
                       gggggAGTCACTGACAAAGGCGCAGGAAGAGGTGATAGAGAGCAAGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATg                                                                                                                       >  4:267600/1‑148 (MQ=255)
                          ggAGTCACTGACAAAGGCGCAGGAAGAGGTGATAGAGAGCAAGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGt                                                                                                                   <  2:116588/149‑1 (MQ=255)
                             gTCACTGACAAAGGCGCAGGAAGAGGTGATAGAGAGCAAGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTggg                                                                                                                <  3:267600/149‑1 (MQ=255)
                                      aaaGGCGCAGGAAGAGGTGATAGAGAGCAAGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGATGACCTTCATACAAGCAAACGa                                                                                                                              >  1:133391/1‑126 (MQ=255)
                                      aaaGGCGCAGGAAGAGGTGATAGAGAGCAAGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGATGACCTTCATACAAGCAAACGa                                                                                                                              >  1:138324/1‑126 (MQ=255)
                                      aaaGGCGCAGGAAGAGGTGATAGAGAGCAAGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGATGACCTTCATACAAGCAAACGa                                                                                                                              <  2:133391/126‑1 (MQ=255)
                                      aaaGGCGCAGGAAGAGGTGATAGAGAGCAAGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGATGACCTTCATACAAGCAAACGa                                                                                                                              <  2:138324/126‑1 (MQ=255)
                                            gcAGGAAGAGGTGATAGAGAGCAAGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCt                                                                                                  >  1:240966/1‑148 (MQ=255)
                                                  agagGTGATAGAGAGCACGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCgg                                                                                           <  8:237852/149‑1 (MQ=255)
                                                  agagGTGATAGAGAGCAAGCACGCGATCTTCGCTTGAAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCgg                                                                                           <  8:110810/149‑1 (MQ=255)
                                                    agGTGATAGAGAGCAAGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCGGGt                                                                                         >  7:177494/1‑149 (MQ=255)
                                                         aTAGAGAGCAAGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCtt                                                                                                                                             >  1:195145/1‑92 (MQ=255)
                                                         aTAGAGAGCAAGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCtt                                                                                                                                             <  2:195145/92‑1 (MQ=255)
                                                             agagCAAGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCGGGTGACTTTCTg                                                                                <  6:200815/149‑1 (MQ=255)
                                                                     caCGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTAc                                                                                                <  8:201759/125‑1 (MQ=255)
                                                                     caCGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTAc                                                                                                >  7:283771/1‑125 (MQ=255)
                                                                     caCGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTAc                                                                                                <  8:283771/125‑1 (MQ=255)
                                                                                cGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCGGGTGACTTTCTGGTACCGGATAGCTTTg                                                                <  2:240966/146‑1 (MQ=255)
                                                                                   ttCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTCGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGGTCTACTTCGGGGGACTTTCTGGTACCCGAAAGCTTTGAAGACa                                                          >  4:133487/1‑149 (MQ=255)
                                                                                       aaCGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCGGGTGACTTTCTGGTACCGGATAGCTTTGAAGACatgat                                                      >  4:275559/1‑149 (MQ=255)
                                                                                           gAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCGGGTGACTTTCTGGTACCGGATAGCTTTGAAGACATGATGATc                                                  <  1:130481/149‑1 (MQ=255)
                                                                                               ggTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCGGGTGACTTTCTGGTACCGGATAGCTTTGAAGACATGATGATCGTGc                                              >  4:142774/1‑149 (MQ=255)
                                                                                                gTCATGGGGAGGCGGCTCGCGAGCTTTCACGTTTTGGGGTATGAGCCGACCTTCATACAAGCTAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCGGGTGACTTTCTGGTACCGGATAGCTTTGAAGACATGATGATCGTGc                                              <  3:133487/148‑1 (MQ=255)
                                                                                                   aaGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTGCTACTTCGGGTGACTTTCTGGTACCGGATAGCTTTGAAGACATGATGATCGTGCCGAt                                          >  5:133647/1‑149 (MQ=255)
                                                                                                               ctCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCGGGTGACTTTCTGGTACCGGATAGCTTTGAAGACATGATGATCGTGCCGATCTCACCCCGttt                              <  4:108288/149‑1 (MQ=255)
                                                                                                               ctCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCGGGTGACTTTCTGGTACCGGATAGCTTTGAAGACATGATGATCGTGCCGATCTCACCCCGttt                              >  3:108288/1‑149 (MQ=255)
                                                                                                                  gcgAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCGGGTGACTTTCTGGTACCGGATAGCTTTGAAGACATGATGATCGTGCCGATCTCACACCGTTTGGc                           <  6:314052/149‑1 (MQ=255)
                                                                                                                    gAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCGGGTGACTTTCTGGTACCGGATAGCTTTGAAGACATGATGATCGTGCCGATCTCACCCCGTTTGGCGa                         >  8:17360/1‑149 (MQ=255)
                                                                                                                     aGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCGGGTGACTTTCTGGTACCGGATAGCTTTGAAGACATGATGATCGTGCCGATCTCACCCCGTTTGGCGAt                        >  6:160311/1‑149 (MQ=255)
                                                                                                                          cgattttttGGGGTATGAGACGACCTTCATACAAGCAAACTTTTCGATGCAGTGGGGCATCGCCCGTTCTACTTCGGGTGACTTTCTGGTACCGGATAGCTTTGAAGACATGATGATCGTGCCGATCTCACCCCGTTTGGCGATCGtt                    <  1:274307/143‑1 (MQ=255)
                                                                                                                           cACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCGGGTGACTTTCTGGTACCGGATAGCTTTGAAGAc                                                           >  4:28904/1‑108 (MQ=255)
                                                                                                                           cACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCGGGTGACTTTCTGGTACCGGATAGCTTTGAAGAc                                                           <  3:28904/108‑1 (MQ=255)
                                                                                                                                 ttGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCGGGTGACTTTCTGGTACCGGATAGCTTTGAAGACATGATGATCGTGCCGATCTCACCCCGTTTGGCGATTGtt                    >  2:274307/1‑141 (MQ=255)
                                                                                                                                      gTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCGGGTGACTTTCTGGTACCGGATAGCTTTGAAGACATGATGATCGTGCCGATCTCACCCCGTTTGGCGATCGTTGCCGGCGTGCCt        >  3:37828/1‑148 (MQ=255)
                                                                                                                                           agaCGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCGGGTGACTTTCTGGTACCGGATAGCTTTGAAGACATGATGATCGTGCCGATCTCACCCCGTTTGGCGATCGTTGCCGGCGTGCCTGACTGc  >  8:30096/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
GATGTGAGGCTACATGGCGTCGAGGGGGAGTCACTGACAAAGGCGCAGGAAGAGGTGATAGAGAGCAAGCACGCGATCTACGCTTCTAACGGAGTGGTCAAGGGGAGGCTGCTCGCGAGCATTCACGTTTTGGGGTATGAGACGACCTTCATACAAGCAAACGAGTCGATGCAGTGGGGCATCGCCCGTTCTACTTCGGGTGACTTTCTGGTACCGGATAGCTTTGAAGACATGATGATCGTGCCGATCTCACCCCGTTTGGCGATCGTTGCCGGCGTGCCTGACTGC  >  NC_002947/2171025‑2171312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑