Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,177,884 C→T 6.4% L40L (CTC→CTT)  PP_1931 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,177,8840CT 6.4% 186.1 / 2.1 62L40L (CTC→CTT) PP_1931hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (33/25);  new base T (2/2);  total (35/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

GTCGTCGTGACCGTGGGGACAATCATGAAGCGATTCATCGAAGGTGAGGCTCGAACGCAGGTTACATTGCTGCCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGTCCAGCCGCCGGCTTGAGCGTGAAGCT  >  NC_002947/2177741‑2178025
                                                                                                                                               |                                                                                                                                             
gtcgtcGTGACCGTGGGGACAATCATGAAGCGATTCATCGAAGGTGAGGCCCGAACGCAGGTTACATTGCTGCCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGa                                                                                                                                             <  5:333879/146‑1 (MQ=255)
   gtcgtGACCGTGGGGACAATCATGAAGCGATTCATCGAAGGTGAGGCTCGAACGCAGGTTACATTGCTGCCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTggg                                                                                                                                       >  5:79069/1‑149 (MQ=255)
   gtcgtGACCGTGGGGACAATCATGAAGCGATTCATCGAAGGTGAGGCTCGAACGCAGGTTACATTGCTGCCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTgg                                                                                                                                        <  1:103684/148‑1 (MQ=255)
         aCCGTGGGGACAATCATGAAGCGATTCATCGAAGGTGAGGCTCGAACGCAGGTTACATTGCTGCCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCt                                                                                                                                 >  5:215784/1‑149 (MQ=255)
                      tCATGAAGCGATTCATCGAAGGTGAGGCTCGAACGCAGGTTACATTGCTGCCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGgt                                                                                                                    <  6:79069/149‑1 (MQ=255)
                         tGAAGCGATTCATCGAAGGTGAGGCTCGAACGCAGGTTACATTGCTGCCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTc                                                                                                                 >  5:167144/1‑149 (MQ=255)
                            aGCGATTCATCGAAGGTGAGGCTCGAACGCAGGTTACATTGCTGCCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGAt                                                                                                              <  8:76169/149‑1 (MQ=255)
                             gCGATTCATCGAAGGTGAGGCTCGAACGCAGGTTACATTGCTGCCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATc                                                                                                             >  7:96782/1‑149 (MQ=255)
                                   cATCGAAGGTGAGGCTCGAACGCAGGTTACATTGCTGCCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGgca                                                                                                        <  6:215784/148‑1 (MQ=255)
                                    aTCGAAGGTGAGGCTCGAACGCAGGTTACATTGCTGCCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGc                                                                                                                        >  3:9601/1‑131 (MQ=255)
                                    aTCGAAGGTGAGGCTCGAACGCAGGTTACATTGCTGCCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGc                                                                                                                        <  4:9601/131‑1 (MQ=255)
                                     tCGAAGGTGAGGCTCGAACGCAGGTTACATTGCTGCCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGgcagc                                                                                                      >  8:113241/1‑148 (MQ=255)
                                       gAAGGTGAGGCTCGAACGCAGGTTACATTGCTGCCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCaa                                                                                                    >  1:72822/1‑148 (MQ=255)
                                                  tctaaCGCAGGTTACATTGCTGCCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCgg                                                                                        >  3:32435/4‑149 (MQ=255)
                                                     aaCGCAGGTTACATTGCTGCCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGcc                                                                                      >  1:109838/1‑148 (MQ=255)
                                                         ccggTTACATTGCTGCCGGACTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGGGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCa                                                                                 <  4:282040/147‑1 (MQ=255)
                                                                 aTTGCTGCCGGAGTGCCTGGACGACTACGTCAGCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCt                                                                                                                                 >  6:142260/1‑93 (MQ=255)
                                                                 aTTGCTGCCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCt                                                                                                                                 <  5:142260/93‑1 (MQ=255)
                                                                       gcCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGc                                                                   >  8:28994/1‑149 (MQ=255)
                                                                        ccGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGc                                                                   <  2:17339/148‑1 (MQ=255)
                                                                         cGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCt                                                                  >  6:134331/1‑148 (MQ=255)
                                                                           gAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGaa                                                               <  4:32435/149‑1 (MQ=255)
                                                                                  tGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGatcta                                                         >  4:145473/1‑148 (MQ=255)
                                                                                    gacgacTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGatctac                                                        <  5:171744/147‑1 (MQ=255)
                                                                                    gacgacTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCATCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTCCCATCCAGCGGTCTTGCTGAAGatctacat                                                      >  1:242078/1‑149 (MQ=255)
                                                                                       gacTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGatctacatcta                                                   >  6:71453/1‑149 (MQ=255)
                                                                                         cTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCAACCAGCGGTCTTGCTGAAGATCTACATCTACg                                                 >  7:223606/1‑149 (MQ=255)
                                                                                              tCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTAc                                            >  8:7339/1‑149 (MQ=255)
                                                                                               cACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTAc                                            >  2:325183/1‑148 (MQ=255)
                                                                                                  cGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCAGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTa                                         >  8:15197/1‑148 (MQ=255)
                                                                                                        aaaCCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTACAccctc                                  >  1:119256/1‑146 (MQ=255)
                                                                                                          aCCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCAtt                                <  3:145473/149‑1 (MQ=255)
                                                                                                          aCCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCAtt                                <  2:72822/149‑1 (MQ=255)
                                                                                                            ccAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCAtt                                >  6:176251/1‑147 (MQ=255)
                                                                                                            ccAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCAtt                                >  2:264427/1‑147 (MQ=255)
                                                                                                            ccAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCa                              <  2:109838/149‑1 (MQ=255)
                                                                                                            ccAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCa                              <  7:113241/149‑1 (MQ=255)
                                                                                                             cAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAg                             <  8:96782/149‑1 (MQ=255)
                                                                                                              aGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGt                            <  7:7339/149‑1 (MQ=255)
                                                                                                                     gtgGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCt                                              <  2:113111/124‑1 (MQ=255)
                                                                                                                     gtgGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCt                                              >  1:113111/1‑124 (MQ=255)
                                                                                                                      tgGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGTCCAgccgc                    >  1:241480/1‑149 (MQ=255)
                                                                                                                           gATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGTCCAGCCGCCGGCt               >  8:261014/1‑149 (MQ=255)
                                                                                                                                 ttCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGatctac                                                        >  4:235257/1‑102 (MQ=255)
                                                                                                                                 ttCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGTCCAGCCGCCGGCTTGAg           >  7:208373/1‑147 (MQ=255)
                                                                                                                                 ttCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGTCCAGCCGCCGGCTTGAg           <  8:208373/147‑1 (MQ=255)
                                                                                                                                 ttCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGTCCAGCCGCCGGCTTGAGCg         >  6:265546/1‑149 (MQ=255)
                                                                                                                                   cgtcgATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGTCCAGCCGCCGGCTTGAGCGt        <  1:325183/148‑1 (MQ=255)
                                                                                                                                   cgtcgATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGTCCAGCCGCCGGCTGGact          <  5:134331/146‑3 (MQ=255)
                                                                                                                                    gtcgATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGTCCAGCCGCCGGCTTGAGCGTg       >  6:268270/1‑148 (MQ=255)
                                                                                                                                     tcgATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCCACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGt                            <  1:321346/126‑1 (MQ=255)
                                                                                                                                     tcgATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCCACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGt                            >  2:321346/1‑126 (MQ=255)
                                                                                                                                      cgATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGTCCAGCCGCCGGCTTGAGCGTGAAg    >  4:296180/1‑149 (MQ=255)
                                                                                                                                       gATGAACTTGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGTCCAGCCGCCGGCTTGAGCGTg       >  4:87815/1‑145 (MQ=255)
                                                                                                                                       gATGAACTTGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGTCCAGCCGCCGGCTTGAGCGTg       >  4:113184/1‑145 (MQ=255)
                                                                                                                                       gATGAACTTGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGTCCAGCCGCCGGCTTGAGCGTg       <  3:113184/145‑1 (MQ=255)
                                                                                                                                       gATGAACTTGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGTCCAGCCGCCGGCTTGAGCGTg       <  3:87815/145‑1 (MQ=255)
                                                                                                                                       gATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGTCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGTCCAGCCGCCGGCTTGAGCGTGAAg    <  7:261014/148‑1 (MQ=255)
                                                                                                                                        aTGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGTCCAGCCGCCGGCTTGAGCGTGAAGCt  <  3:296180/149‑1 (MQ=255)
                                                                                                                                             cTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACgg                                                <  6:24717/98‑1 (MQ=255)
                                                                                                                                             cTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACgg                                                >  5:24717/1‑98 (MQ=255)
                                                                                                                                              tCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCa                                  >  8:43296/1‑111 (MQ=255)
                                                                                                                                               |                                                                                                                                             
GTCGTCGTGACCGTGGGGACAATCATGAAGCGATTCATCGAAGGTGAGGCTCGAACGCAGGTTACATTGCTGCCGGAGTGCCTGGACGACTACGTCACCGATGAAAACCCAGTGCGTGTGGTCGATGTCTTCGTCGATGAACTCGACCTGGGCGCGCTCGATTTCGCAGGTGTCGATCCGGCAGCAACAGGTCGCCCGGCCTACCATCCAGCGGTCTTGCTGAAGATCTACATCTACGGCTACCTAAACCGCATTCAGTCCAGCCGCCGGCTTGAGCGTGAAGCT  >  NC_002947/2177741‑2178025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑