Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,179,314	T→A	9.9%	intergenic (+113/+155)	PP_1931 → / ← PP_5486	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,179,314	0	T	A	9.9%	131.0 / 2.6	47	intergenic (+113/+155)	PP_1931/PP_5486	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (22/20);  new base A (3/2);  total (25/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCAAGGGGCTGCTCAAGGCGATCAAGGCGTGAACTGGCCTACTGAGGCGCCGGTATGCATGCCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGATCATCAGCGAAAGGGCTAGCCTCGCTTTCCGCAAATGCCCGACCGTATCGATGGGGTTTCTAG  >  NC_002947/2179168‑2179459                                                                                                                                                   |                                                                                                                                                  cATCAAGGGGCTGCTCAAGGCGATCAAGGCGTGAACTGGCGTACTGAGGCGCCGGTATGCATGCCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGttt                                                                                                                                                 <  2:77657/149‑1 (MQ=255) cATCAAGGGGCTGCTCAAGGCGATCAAGGCGTGAACTGGCCTACTGAGGCGCCGGTATGCATGCCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGttt                                                                                                                                                 <  5:212878/149‑1 (MQ=255)  aTCAAGGGGCTGCTCAAGGCGATCAAGGCGTGAACTGGCCTACTGAGGCGCCGGTATGCATGCCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTg                                                                                                                                                <  8:158339/149‑1 (MQ=255)     aaGGGGCTGCTCAAGGCGATCAAGGCGTGAACTGGCCTACTGAGGCGCCGGTATGCATGCCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGc                                                                                                                                              <  5:259520/148‑1 (MQ=255)        gggCTGCTCAAGGCGATCAAGGCGTGAACTGGCCTACTGAGGCGCCGGTATGCATGCCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGc                                                                                                                                          <  7:287002/149‑1 (MQ=255)           ctgctCAAGGCGATCAAGGCGTGAACTGGCCTACTGAGGCGCCGGTATGCATGCCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCctc                                                                                                                                       <  4:193004/149‑1 (MQ=255)              ctCAAGGCGATCAAGGCGGGACCTGCCCTACTGAGGCGCCGGTATGCATCCCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCACCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCctct                                                                                                                                      <  8:78894/147‑1 (MQ=255)                  aGGCGATCAAGGCGTGAACTGGCCTACTGAGGCGCCGGTATGCATGCCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCctctc                                                                                                                                     >  8:277305/1‑144 (MQ=255)                      gATCAAGGCGTGAACTGGCCTACTGAGGCGCCGGTATGCATGCCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATccat                                                                                                                            >  1:11758/1‑147 (MQ=255)                          aaGGCGTGAACTGGCCTACTGAGGCGCCGGTATGCATGCCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTgcgc                                                                                                                        >  6:151518/1‑149 (MQ=255)                          aaGGCGTGAACTGGCCTACTGAGGCGCCGGTATGCATGCCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTgcgc                                                                                                                        >  4:136254/1‑149 (MQ=255)                          aaGGCGTGAACTGGCCTACTGAGGCGCCGGTATGCATGCCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAAACTGGCAAgaaatt                                                                                                                                      >  4:216214/1‑130 (MQ=255)                          aaGGCGTGAACTGGCCTACTGAGGCGCCGGTATGCATGCCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAAACTGGCAAgaaatt                                                                                                                                      <  3:216214/135‑6 (MQ=255)                                                    ccGGTATGCATGCCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCGCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCCAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTca                                                                                              <  6:155090/149‑1 (MQ=255)                                                       gTATGCATGCCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTc                                                                                               >  5:59172/1‑145 (MQ=255)                                                       gTATGCATGCCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTc                                                                                               <  6:59172/145‑1 (MQ=255)                                                              tgcCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTgg                                                                                    >  5:246889/1‑149 (MQ=255)                                                                cccAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTggg                                                                                   <  8:125967/148‑1 (MQ=255)                                                                cccAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTggg                                                                                   <  2:318945/148‑1 (MQ=255)                                                                          cTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGcggc                                                                        >  5:217230/1‑149 (MQ=255)                                                                           tCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTgg                                                                                                                                               <  4:170281/77‑1 (MQ=255)                                                                           tCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTgg                                                                                                                                               >  3:170281/1‑77 (MQ=255)                                                                           tCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGcggcc                                                                       >  1:318958/1‑149 (MQ=255)                                                                             aaGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGcggccg                                                                      <  3:136254/148‑1 (MQ=255)                                                                                    aCTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGcggcc                                                                       >  7:271478/1‑140 (MQ=255)                                                                                    aCTCACATCGCCACATGCCCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGcggcc                                                                       <  8:271478/140‑1 (MQ=255)                                                                                     cTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGa                                                                 <  7:217987/145‑1 (MQ=255)                                                                                     cTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGa                                                                 >  8:217987/1‑145 (MQ=255)                                                                                        acaTCGCCACATGCGCCCCAGCACGGCGATTTGAGCTACACCACGCAAATTCTTGCCAGTTTGGCAAGCATCTCTAGATCCCTGCGCCCTTCAACGCATTTCGAGAGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGatcgtgcg                                                          >  8:93621/1‑144 (MQ=255)                                                                                        acaTCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGatcatc                                                            <  6:246889/147‑1 (MQ=255)                                                                                        acaTCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGatcat                                                             >  2:320572/1‑146 (MQ=255)                                                                                        acaTCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGAtcatca                                                           <  1:320572/148‑1 (MQ=255)                                                                                        acaTCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGAtcatca                                                           <  5:151518/148‑1 (MQ=255)                                                                                        acaTCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGATTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGatcatc                                                            <  8:148166/147‑1 (MQ=255)                                                                                                      gcCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGcgg                                                                         <  6:245432/120‑1 (MQ=255)                                                                                                         ccAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGATCATCAGCGAAAGGGCTAGCCTCg                                         >  2:58584/1‑149 (MQ=255)                                                                                                                           cTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGATCATCAGCGAAAGGGCTAGCCTCGCTTTCCGCAAATGCCCGa                       >  3:44313/1‑149 (MQ=255)                                                                                                                              aaCCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGATCATCAGCGAAAGGGCTAGCCTCGCTTTCCGCAAATGCCCGACCg                    >  7:130719/1‑149 (MQ=255)                                                                                                                                 cACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGATCATCAGCGAAAGGGCTa                                               >  8:206160/1‑119 (MQ=255)                                                                                                                                 cACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGCCCGATCATCAGCGAAAGGGCTa                                               <  7:206160/119‑1 (MQ=255)                                                                                                                                    gCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGATCATCAGCGAAAGGGCTAGCCTCGCTTTCCGCAAATGCCCGACCGTATCGa              >  5:296587/1‑149 (MQ=255)                                                                                                                                      aaTTTCTTGCCAGTTTGGCAAGCGTCTCTAGATCCCTGCGCCCTTCACCGCATTTCGCGTGTGTTCACACAGCCTGGGTCGCTAGCGGCCGGCCGATCATCAGCGAAAGGGGTAGCCTCGCGTTCCGCCAGTGCCCGCCCGTATCGagg            >  4:228703/1‑147 (MQ=255)                                                                                                                                         ttCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGATCATCAGCGAAAGGGCTAGCCTCGCTTTCCGCAAATGCCCGACCGTATCGATgggg         >  3:150023/1‑149 (MQ=255)                                                                                                                                         ttCTTGCCAGTTTGGAAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGATCATCAGCGAAAGGGCTAGCCTCGCTTTCCGCAAATGCCCGACCGTATCGATgggg         >  6:136235/1‑149 (MQ=255)                                                                                                                                          gcTTGCCAAACTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGATCATCAGCGAAAGGGCTAGCCTCGCTTTCCGCAAATGCCCGACCGTATCGATGGGGt        >  7:167584/2‑149 (MQ=255)                                                                                                                                          gcTTGCCAAACTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGATCATCAGCGAAAGGGCTAGCCTCGCTTTCCGCAAATGCCCGACCGTATCGATGGGGt        >  5:255097/2‑149 (MQ=255)                                                                                                                                              gCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGATCATCAGCGAAAGGGCTAGCCTCGCTTTCCGCAAATGCCCGACCGTATCGATGGGGTTTc     <  2:11758/148‑1 (MQ=255)                                                                                                                                                cAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGATCATCAGCGAAAGGGCTAGCCTCGCTTTCCGCAAATGCCCGACCGTATCGATGGGGTTTCTAg  <  7:277305/149‑1 (MQ=255)                                                                                                                                                  gTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGATCATCAGCGAAAGGGCTAGCCTCGCTTTCCGCAAATGCCCGACCGTATCGATGGGGTTTCTAg  >  7:27554/1‑147 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CATCAAGGGGCTGCTCAAGGCGATCAAGGCGTGAACTGGCCTACTGAGGCGCCGGTATGCATGCCCAAAAGACCTCAAGAACGACTCACATCGCCACATGCGCCCCAGCACGGCGATTTGAGCTAAACCACGCAATTTCTTGCCAGTTTGGCAAGCCTCTCTAGATCCCTGCGCCCTTCAACGCATTTCGCGTGTTTTCACACAGCCTGGGTCGATAGCGGCCGGCCGATCATCAGCGAAAGGGCTAGCCTCGCTTTCCGCAAATGCCCGACCGTATCGATGGGGTTTCTAG  >  NC_002947/2179168‑2179459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑