Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,240,327	C→T	7.3%	L186L (CTG→TTG)	gltX →	glutamate‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,240,327	0	C	T	7.3%	159.4 / 2.7	55	L186L (CTG→TTG)	gltX	glutamate‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (21/30);  new base T (2/2);  total (23/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

CCGAAGAAGTGCAGCGCCGCCTGGACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAG  >  NC_002947/2240187‑2240473                                                                                                                                             |                                                                                                                                                     ccGAAGAAGTGCAGCGCCGCCTGGACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCt‑gatg                                                                                                                                                >  7:195621/1‑146 (MQ=255)     agaagTGCAGCGCCGCCTGGACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑acg                                                                                                                                        >  3:44085/1‑149 (MQ=255)     agaagTGCAGCGCCGCCTGGACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑acg                                                                                                                                        >  5:200255/1‑149 (MQ=255)        agTGGAGCGCCGCCGGGACGCGGGCGAGCCGCACGTGCTCCGCCTGAAGGTGCCGAGCGAAGGGATCGGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑acgacg                                                                                                                                     <  4:35144/149‑1 (MQ=255)         gTGCAGCGCCGCCTGGACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACgg                                                                                                                                    >  7:292533/1‑149 (MQ=255)                gccgccTGGACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCGGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCg                                                                                                                             <  3:230233/149‑1 (MQ=255)                 ccgccTGGACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑CCGACGGCCTGCCGa                                                                                                                            >  4:197693/1‑149 (MQ=255)                            cgggCGAGCCGCACGTGATCCGCATGAAGGGGCCGAGCGCAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTg                                                                                                                 <  8:292533/147‑1 (MQ=255)                               gCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTgg                                                                                                                <  4:136810/147‑1 (MQ=255)                               gCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGc                                                                                                               <  6:200255/148‑1 (MQ=255)                                   gCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACg                                                                                                          >  1:116201/1‑149 (MQ=255)                                      gcccGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCACGTCGGACCGCATGGACATGCAGGTGTT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTgg                                                                                                       >  7:60800/4‑149 (MQ=255)                                      gCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGTT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTgg                                                                                                       >  7:60799/1‑149 (MQ=255)                                       cACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGt                                                                                                      >  2:53673/1‑149 (MQ=255)                                           tGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTcgac                                                                                                  <  8:195621/149‑1 (MQ=255)                                             aTCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCTGCGATGTGTTTTTCTCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTcgacga                                                                                                <  1:107474/149‑1 (MQ=255)                                              tCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCt‑ga                                                                                                                                                  >  7:37820/1‑99 (MQ=255)                                              tCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCt‑ga                                                                                                                                                  <  8:37820/99‑1 (MQ=255)                                                  cATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGTCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGaccacc                                                                                           >  1:199985/1‑149 (MQ=255)                                                  cATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGaccacc                                                                                           >  7:247432/1‑149 (MQ=255)                                                      aaGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGa                                                                                        <  2:259691/148‑1 (MQ=255)                                                        ggTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATgg                                                                                     <  8:91670/149‑1 (MQ=255)                                                        ggTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATgg                                                                                     <  4:217306/149‑1 (MQ=255)                                                        ggTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATgg                                                                                     <  1:53673/149‑1 (MQ=255)                                                         gTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATggg                                                                                    >  7:33825/1‑149 (MQ=255)                                                              gAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGATCCGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATc                                                                                <  1:95357/149‑1 (MQ=255)                                                                  gAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCa                                                                           >  4:9840/1‑149 (MQ=255)                                                                    aGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACg                                                                         >  3:186893/1‑149 (MQ=255)                                                                        aTCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGtgct                                                                     >  7:127269/1‑149 (MQ=255)                                                                               tggtgCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTggg                                                              <  8:247432/149‑1 (MQ=255)                                                                                   gCCGGACATGCTGCGCGGCGATGTCGAGATCCTGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTgggg                                                             >  4:191439/1‑146 (MQ=255)                                                                                      ggACATGCTGCGCGGCGATGTCGAGATCCTGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGt                                                       <  3:191439/149‑1 (MQ=255)                                                                                        aCATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTgg                                                     <  4:186893/149‑1 (MQ=255)                                                                                               gcgcGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCTGGATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCa                                               >  1:77583/1‑149 (MQ=255)                                                                                                cgcgGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATc                                             <  8:33825/149‑1 (MQ=255)                                                                                                 gcggcgATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCg                                            <  3:197693/149‑1 (MQ=255)                                                                                                    gcgATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGgcg                                         <  2:188408/149‑1 (MQ=255)                                                                                                     cgATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGaa                                                           <  5:147459/130‑1 (MQ=255)                                                                                                     cgATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGaa                                                           >  6:147459/1‑130 (MQ=255)                                                                                                     cgATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGgcgc                                        <  2:77583/149‑1 (MQ=255)                                                                                                            gagaTCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGaa                                                           <  4:275878/123‑1 (MQ=255)                                                                                                            gagaTCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCt                                 >  1:245206/1‑149 (MQ=255)                                                                                                            gagaTCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCt                                 <  2:245206/149‑1 (MQ=255)                                                                                                              gaTCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGa                               <  2:199985/149‑1 (MQ=255)                                                                                                                tCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATc                             <  1:38821/149‑1 (MQ=255)                                                                                                                   cGTGGTACGGAATGGACATGCAGTTGCT‑GATGAAGA‑ACGACGGCCTGCCGAGGTACTTCCTGGCCAACGTTGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCaaa                          <  2:116201/149‑1 (MQ=255)                                                                                                                   cGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCt                                 <  4:194900/142‑1 (MQ=255)                                                                                                                   cGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCt                                 >  3:194900/1‑142 (MQ=255)                                                                                                                     tGGGACCGCATGGACATGCAGGTGTT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACt                        <  8:60799/149‑1 (MQ=255)                                                                                                                     ggggACCGCCTGGACATGCAGGTGTG‑TATGAAGT‑ACGACGGCCTCGCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACt                        <  8:60800/148‑1 (MQ=255)                                                                                                                        gACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTa                     <  8:127269/149‑1 (MQ=255)                                                                                                                              aTGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTa               <  3:245453/149‑1 (MQ=255)                                                                                                                                      gCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCt       >  1:110095/1‑149 (MQ=255)                                                                                                                                          gTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGa   <  3:213341/149‑1 (MQ=255)                                                                                                                                           tGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGag  >  4:114044/1‑149 (MQ=255)                                                                                                                                             |                                                                                                                                                     CCGAAGAAGTGCAGCGCCGCCTGGACGCTGGCGAGCCGCACGTGATCCGCATGAAGGTGCCGAGCGAAGGGATCTGCGTGGTGCCGGACATGCTGCGCGGCGATGTCGAGATCCCGTGGGACCGCATGGACATGCAGGTGCT‑GATGAAGA‑ACGACGGCCTGCCGACGTACTTCCTGGCCAACGTGGTCGACGACCACCTGATGGGCATCACCCACGTGCTGCGTGGGGAAGAGTGGCTGCCATCGGCGCCCAAGCTGATCAAACTGTACGAGTACTTCGGCTGGGAG  >  NC_002947/2240187‑2240473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑