| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,270,150 | C→T | 45.2% | A318V (GCA→GTA) | metZ → | O‑succinylhomoserine sulfhydrylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,270,150 | 0 | C | T | 45.2% | 27.2 / 112.5 | 93 | A318V (GCA→GTA) | metZ | O‑succinylhomoserine sulfhydrylase |
| Reads supporting (aligned to +/- strand): ref base C (21/30); new base T (20/22); total (41/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-02 | |||||||||||
CTGCGTATGCGTGCGCACTGCGAAAGCGCTCAGGCCCTGGCCGAATGGCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAACCACCATCGCTCATCCGGCGACCACCTCACACGGTCGTCTGTCGCC > NC_002947/2270005‑2270297 | cttcGTATGCGTGCGGACTGCGAAAGCGCCCAGGCGCTGGCCGTATGGCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCAGGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGt < 5:210423/146‑1 (MQ=255) cGTATGCGTGCGCACTGCGAAAGCGCTCAGGCCCTGGCCGAATGGCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAACGCCAGATGAGCGGTTTTGGTGCAGTg < 5:329649/147‑1 (MQ=255) cgtgcgCACTGCGAAAGCGCTCAGGCCCTGGCCGAATGGCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCt < 2:60004/148‑1 (MQ=255) cgtgcgCACTGCGAAAGCGCTCAGGCCCTGGCCGAATGGCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCt < 5:292426/148‑1 (MQ=255) gtgcgCACTGCGAAAGCGCTCAGGCCCTGGCCGAATGGCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCa > 4:34410/1‑144 (MQ=255) gtgcgCACTGCGAAAGCGCTCAGGCCCTGGCCGAATGGCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCttt > 6:305450/1‑149 (MQ=255) gtgcgCACTGCGAAAGCGCTCAGGCCCTGGCCGAATGGCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCttt < 5:305450/149‑1 (MQ=255) gtgcgCACTGCGAAAGCGCTCAGGCCCTGGCCGAATGGCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGCGCAGTGGTCa < 3:34410/144‑1 (MQ=255) cgcACTGCGAAAGCGCTCAGGCCCTGGCCGAATGGCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGaa < 8:28159/149‑1 (MQ=255) cgcACTGCGAAAGCGCTCAGGCCCTGGCCGAATGGCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGaa < 5:173409/149‑1 (MQ=255) cgcACTGCGAAAGCGCTCAGGCCCTGGCCGAATGGCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGaa < 5:23612/149‑1 (MQ=255) cTGCGAAAGCGCTCAGGCCCTGGCCGAATGGCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCa < 2:303851/149‑1 (MQ=255) gcTCAGGCCCTGGCCGAATGGCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCa > 7:280448/1‑148 (MQ=255) ggCCCTGGCCGAATGGCTGGAGCAGCAGGACGGCGGGGAGAAGGTGCATTACGCCGGCCGGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAagatg < 6:290827/149‑3 (MQ=255) cTGGCCGAATGGCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGgcgc < 8:108159/149‑1 (MQ=255) ggCCGAATGGCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCt > 5:67199/1‑149 (MQ=255) gCCGAATGGCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTg > 3:280167/1‑149 (MQ=255) aaTGGCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGt > 4:11573/1‑149 (MQ=255) aTGGCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGtt < 2:52504/149‑1 (MQ=255) gCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTc < 3:145855/148‑1 (MQ=255) cTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAAGGGGGGGCAAAGAGGGCGCCTGGCGTTTCa > 2:120219/1‑149 (MQ=255) cTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTcc > 8:118029/1‑147 (MQ=255) gAGCAGCTTGACGGGGTGGAGAAGGTTCATTACGCCGGCCTGCCCAGCCACCCTCAGCACGAACTGGGCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCgt < 2:14359/149‑2 (MQ=255) gAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATc < 5:163707/147‑1 (MQ=255) agcagcAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGAc < 7:98545/149‑1 (MQ=255) gcagGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTa > 1:223312/1‑149 (MQ=255) agGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTAcc > 1:157978/1‑149 (MQ=255) ggACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCaaa < 6:161969/118‑1 (MQ=255) ggACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCaaa > 5:161969/1‑118 (MQ=255) ggACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTAccc < 6:67199/149‑1 (MQ=255) gACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACg < 3:303540/142‑1 (MQ=255) gACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACg > 4:303540/1‑142 (MQ=255) aCGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGa < 7:86761/149‑1 (MQ=255) aCGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGa < 1:67527/149‑1 (MQ=255) aCGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGa < 3:24969/149‑1 (MQ=255) gTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAagag > 4:5850/1‑114 (MQ=255) gTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAagag < 3:5850/114‑1 (MQ=255) gagaAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGAttt < 3:11573/148‑1 (MQ=255) gagaAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGAttt < 5:161256/148‑1 (MQ=255) aGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAAGGGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCAt < 1:120219/149‑1 (MQ=255) aGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGCGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATc < 7:213094/125‑1 (MQ=255) aGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGAGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATc > 8:213094/1‑125 (MQ=255) ggTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCa < 7:31896/149‑1 (MQ=255) gTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGt > 8:256576/1‑77 (MQ=255) gTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTGTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCAc > 5:58289/1‑149 (MQ=255) tGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCAc > 1:328468/1‑148 (MQ=255) ttACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGt > 4:82315/1‑132 (MQ=255) ttACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGt < 3:82315/132‑1 (MQ=255) gCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCt > 7:4562/1‑149 (MQ=255) cGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTgg < 8:96876/149‑1 (MQ=255) cGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGGGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTgg < 8:280448/149‑1 (MQ=255) gCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGtt > 6:46964/1‑101 (MQ=255) gCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGtt < 5:46964/101‑1 (MQ=255) gccAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCCTCGCCGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAg > 7:247633/2‑149 (MQ=255) cccAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGAc < 6:56381/147‑1 (MQ=255) cccAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGAc < 2:157978/147‑1 (MQ=255) cccAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGAc > 5:56381/1‑147 (MQ=255) cccAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAg < 3:89528/149‑1 (MQ=255) cccAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GTGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACg < 5:80443/132‑1 (MQ=255) cccAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGCTATTAGTACACTGGTCATCTTTTAAATAAA‑GTTGGGCAAAGAGAGAGCGTGGCGTTACAACGACGCTACACAACTGATTTCCATAACg > 6:80443/1‑132 (MQ=255) cAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGc > 3:86697/1‑148 (MQ=255) ccGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTg > 2:307824/1‑86 (MQ=255) ccGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTg < 1:307824/86‑1 (MQ=255) cGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAg > 5:69289/1‑139 (MQ=255) cGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAg < 6:69289/139‑1 (MQ=255) caCGAACTGGCCAAGCGCCAGATGAGCGGTGTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAACCACCATCGc < 6:58289/149‑1 (MQ=255) aaCTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCa < 3:110741/108‑1 (MQ=255) aaCTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCa > 4:110741/1‑108 (MQ=255) aaCTGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAACCACCATCGCTCAt > 1:314833/1‑149 (MQ=255) aaCTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAac > 5:239569/1‑136 (MQ=255) aaCTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAac < 6:239569/136‑1 (MQ=255) tGGCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAACCACCATCGCTCATCCg > 3:82374/1‑149 (MQ=255) tGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGt > 1:290092/1‑122 (MQ=255) tGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGt < 2:290092/122‑1 (MQ=255) tGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAACCACCATCGCTCATCCg > 8:195304/1‑149 (MQ=255) gCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAACCACCATCGCTCATCCGGc > 5:119960/1‑149 (MQ=255) gCCAAGCGCCAGATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAACCACCATCGCTCATCCGGc < 4:86697/149‑1 (MQ=255) aaGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGt > 8:171932/1‑92 (MQ=255) aaGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGt < 7:171932/92‑1 (MQ=255) aaGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAACCACCATCGCTCATCCGGCGac > 1:236641/1‑149 (MQ=255) gcCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACAAACCTGGGTGACAGCAAAACCACCATCGc > 5:307211/1‑133 (MQ=255) gcCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACAAACCTGGGTGACAGCAAAACCACCATCGc < 6:307211/133‑1 (MQ=255) gATGAGCGGTTTTGGTGTAGTGGTCAGCTTTGGAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAACCACCATCGCTCATCCGGCGACCACCTca > 6:216239/1‑148 (MQ=255) tGAGCGGTTTTGGTGTAGTGGGCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAACCACCATCGCTCATCCGGCGACCACCTCacac < 2:73305/149‑1 (MQ=255) tGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAACCACCATCGCTCATCCGGCGACCACCTCacac > 7:214843/1‑149 (MQ=255) aGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTCTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAACCACCATCGCTCATCCGGCGACCACCTCACACgg < 4:166144/149‑1 (MQ=255) gTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAACCACCATCGCTCATCCGGCGACCACCTCACACGgtcgt > 3:248798/1‑149 (MQ=255) ttttGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAACCACCATCGCTCATCCGGCGACCACCTCACACGgtcgtc < 2:236641/149‑1 (MQ=255) ttGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAACCACCATCGCTCATCCGGCGACCACCTCACACGGTCGTCt < 7:174753/148‑1 (MQ=255) gtgtAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAACCACCATCGCTCATCCGGCGACCACCTCACACGGTCGTCTGTc < 2:85988/148‑1 (MQ=255) gTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAACCACCATCGCTCATCCGGCGACCACCTCACACGGTCGTCTGTc < 7:195304/148‑1 (MQ=255) gCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTg < 1:278587/90‑1 (MQ=255) gCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTg > 2:278587/1‑90 (MQ=255) gCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAACCACCATCGCTCATCCGGCGACCACCTCACACGGTCGTCTGTcgcc > 6:244968/1‑149 (MQ=255) | CTGCGTATGCGTGCGCACTGCGAAAGCGCTCAGGCCCTGGCCGAATGGCTGGAGCAGCAGGACGGCGTGGAGAAGGTGCATTACGCCGGCCTGCCCAGCCACCCGCAGCACGAACTGGCCAAGCGCCAGATGAGCGGTTTTGGTGCAGTGGTCAGCTTTGAAGTCAA‑GGGGGGCAAAGAGGGCGCCTGGCGTTTCATCGACGCTACCCGAGTGATTTCCATCACGACCAACCTGGGTGACAGCAAAACCACCATCGCTCATCCGGCGACCACCTCACACGGTCGTCTGTCGCC > NC_002947/2270005‑2270297 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |