| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,282,770 | T→G | 20.6% | intergenic (‑162/+67) | PP_2010 ← / ← PP_2011 | cytochrome b561/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,282,770 | 0 | T | G | 20.6% | 136.1 / 32.6 | 63 | intergenic (‑162/+67) | PP_2010/PP_2011 | cytochrome b561/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (22/28); new base G (6/7); total (28/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.89e-01 | |||||||||||
GGTCTGAATGGCGCTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCAT > NC_002947/2282627‑2282913 | ggTCTGAATGGCGCTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTg > 8:95837/1‑148 (MQ=255)ggTCTGAATGGCGCTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGGCttcc < 3:238593/149‑3 (MQ=255) tCTGAATGGCGCTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGaa < 7:171419/149‑1 (MQ=255) tGAATGGCGCTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGc > 5:64671/1‑149 (MQ=255) aaTGGCGCTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGc < 4:30146/147‑1 (MQ=255) aaTGGCGCTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCt > 3:268743/1‑149 (MQ=255) aTGGCGCTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTAGGAAGCCtg > 5:156052/1‑149 (MQ=255) tGGCGCTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCtgt < 7:227944/149‑1 (MQ=255) gcgcTCTTGCTGGCGCGCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGc < 2:104904/149‑1 (MQ=255) cgcTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCa < 4:268743/149‑1 (MQ=255) cgcTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCa < 5:265277/149‑1 (MQ=255) cgcTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCCCTTCGCGGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCa < 8:261911/149‑1 (MQ=25) ctTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTg > 3:42547/1‑149 (MQ=255) ttGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGtt < 6:64671/147‑1 (MQ=255) ttGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGtt < 4:309165/147‑1 (MQ=255) ttGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGtt < 2:223299/147‑1 (MQ=255) ttGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGtt < 7:95837/147‑1 (MQ=255) ttGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGtt < 1:4758/147‑1 (MQ=255) gCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGtt < 1:73019/145‑1 (MQ=255) gCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGtt > 2:73019/1‑145 (MQ=255) gCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTg < 6:299707/146‑1 (MQ=255) cTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACt < 3:286142/149‑1 (MQ=255) gcgcTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACtgtg < 4:30439/149‑1 (MQ=255) gcgcTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACtgt < 5:201292/148‑1 (MQ=255) cgcTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTgg < 4:42547/149‑1 (MQ=255) gcTCAGGAGTCCTTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGGGAAAAATGGGTTGCCAGCCACCGGCCTCTTCGCCGGCACGCCCTGCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTgg < 1:251976/149‑1 (MQ=37) aGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACtgtg < 1:303116/143‑1 (MQ=255) aGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACtgtg > 2:303116/1‑143 (MQ=255) aGTCATTTTATTGGCTAACGGAAACAGTTCTTTGGTCAAGGTCTGTGAAAAATGTGTTGCCATGCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCTGTTGGACTGTGGGAGCggg < 8:174284/148‑1 (MQ=25) gTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGAggg > 7:174284/1‑147 (MQ=255) tCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTg > 5:6948/1‑115 (MQ=255) tCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTg < 6:6948/115‑1 (MQ=255) aaTGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTg > 3:76272/1‑124 (MQ=255) aaTGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTg < 4:76272/124‑1 (MQ=255) aGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTgg < 7:106970/93‑1 (MQ=255) aGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTgg > 8:106970/1‑93 (MQ=255) gATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGtt < 8:167970/106‑1 (MQ=255) gATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGtt > 7:167970/1‑106 (MQ=255) tgAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCtt > 2:47324/1‑71 (MQ=255) tgAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCtt < 1:47324/71‑1 (MQ=255) aaaaTGTGTTCCCCGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCCCAGTCCAACTGCACAGTCTTGGATGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGAt < 6:156052/148‑1 (MQ=255) gCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGcc > 8:255228/1‑149 (MQ=255) ccACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGaa > 8:71959/1‑67 (MQ=255) ccACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGaa < 7:71959/67‑1 (MQ=255) ccACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCATCAGAAACACCCGTTCTGCCTCCTGc > 6:225929/1‑147 (MQ=255) ccACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGcctcct < 4:181527/145‑1 (MQ=255) ccACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGcctcct > 3:181527/1‑145 (MQ=255) ccACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCAGCGAAGAGGCCGGTGCAGGCGACATCAGTTACCGGGCAGATAGGGc < 5:7477/108‑1 (MQ=14) ccACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCAGCGAAGAGGCCGGTGCAGGCGACATCAGTTACCGGGCAGATAGGGc > 6:7477/1‑108 (MQ=14) ccACAGTCCAACTGCACAGGCTTCCAAGACTGTGCAGTTGGACGGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTTCCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGc > 5:257569/1‑147 (MQ=25) aGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTg > 7:46766/1‑142 (MQ=255) aGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTg < 8:46766/142‑1 (MQ=255) ccAACTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCa > 2:127469/1‑82 (MQ=14) ccAACTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCa < 1:127469/82‑1 (MQ=14) aaCTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCAGCGAAGAGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCg < 2:5562/125‑1 (MQ=21) aCTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCg > 1:24695/1‑149 (MQ=255) cTGCACAGGCTTCCAAGACTGTGCAGTTGGACTGGGGGAGCGGGCGTTCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACAAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCgg > 3:61716/1‑149 (MQ=37) tGCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCCTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCACGCACCATCgg > 7:237529/1‑148 (MQ=37) gCACAGGCTTCCAAGACTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCAGCGAAGAGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCa > 7:83760/1‑149 (MQ=37) cacaGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCATCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCAt < 5:225929/149‑1 (MQ=255) cacaGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCAt > 3:51410/1‑149 (MQ=255) cacaGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCAt > 1:137978/1‑149 (MQ=255) caGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCAt > 4:141773/1‑147 (MQ=255) | GGTCTGAATGGCGCTCTTGCTGGCGCTCAGGAGTCATTTTAATGGCTACCGGAAACAGATCTTTGCGCCAGGTCTGTGAAAAATGTGTTGCCAGCCACCGGCCTCTTCGCTGGCACGCCC‑GCTCCCACAGTCCAACTGCACAGTCTTGGAAGCCTGTGCAGTTGGACTGTGGGAGCGGGCGTGCCCGCGAAGGGGCCGGAGCAGGCGACATCAGTTACCGGGCAGATAGGGCCAGTCATCACCCACCAGAAACACCCGTTCTGCCTCCTGCCAGGCACCATCGGCAT > NC_002947/2282627‑2282913 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |