BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,287,688	T→A	6.7%	L272* (TTG→TAG)	PP_2016 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,287,688	0	T	A	6.7%	328.2 / 7.4	109	L272* (TTG→TAG)	PP_2016	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (53/48); new base A (5/3); total (58/51)
Fisher's exact test for biased strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

CCCGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGTT  >  NC_002947/2287542‑2287828

cccGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGtt                                                                                                                                               <  8:275970/147‑1 (MQ=255)

cccGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTgg                                                                                                                                             >  3:304452/1‑149 (MQ=255)

cccGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTgg                                                                                                                                             >  2:4973/1‑149 (MQ=255)

  cGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGcc                                                                                                                                           >  4:312486/1‑149 (MQ=255)

    gTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑Gt                                                                                                                                        <  1:4973/149‑1 (MQ=255)

    gTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCAACTGGTAGGCC‑Gt                                                                                                                                        <  6:138356/149‑1 (MQ=255)

    gTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCAACTGGTAGGCC‑Gt                                                                                                                                        <  5:244116/149‑1 (MQ=255)

       gAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGc                                                                                                                                      <  6:22379/148‑1 (MQ=255)

       gAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCt                                                                                                                                     <  4:178367/149‑1 (MQ=255)

         ggCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTcc                                                                                                                                   <  6:116872/149‑1 (MQ=255)

             tACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTc                                                                                                                                    >  7:104905/1‑144 (MQ=255)

             tACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTc                                                                                                                                    <  8:104905/144‑1 (MQ=255)

              aCCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGATGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCAACTGGTAGGCC‑GTgtac                                                                                                                                    <  8:200726/143‑4 (MQ=255)

              aCCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCAACTGGTAGGCC‑GTgtactaga                                                                                                                                >  7:200726/1‑140 (MQ=255)

                ccGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTg                                                                                                                                       >  4:1102/1‑138 (MQ=255)

                ccGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTg                                                                                                                                       <  3:1102/138‑1 (MQ=255)

                   aagaagATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAg                                                                                                                          >  5:140627/1‑148 (MQ=255)

                     gaagaTTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGAc                                                                                                                       <  6:208899/149‑1 (MQ=255)

                                gCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTg                                                                                                                                       >  5:272857/1‑122 (MQ=255)

                                gCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTg                                                                                                                                       <  6:272857/122‑1 (MQ=255)

                                gCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCg                                                                                                            >  8:294726/1‑149 (MQ=255)

                                  ttCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTa                                                                                                          >  5:75483/1‑149 (MQ=255)

                                    cAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTAcc                                                                                                        >  2:14089/1‑149 (MQ=255)

                                       ccTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGaa                                                                                                     >  6:285078/1‑149 (MQ=255)

                                       ccTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGaa                                                                                                     >  3:126422/1‑149 (MQ=255)

                                       ccTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGaa                                                                                                     >  1:290616/1‑149 (MQ=255)

                                        cTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGaac                                                                                                    <  4:304452/149‑1 (MQ=255)

                                        cTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGAACATGCGAACCGaa                                                                                                     >  8:302817/1‑148 (MQ=255)

                                            aCAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAAcc                                                                                                <  5:96877/149‑1 (MQ=255)

                                              aaGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCg                                                                                               >  1:129763/1‑148 (MQ=255)

                                              aaGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCg                                                                                               >  1:133779/1‑148 (MQ=255)

                                              aaGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGc                                                                                              <  2:133779/149‑1 (MQ=255)

                                                 gACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACAt                                                                                                               <  5:212317/129‑1 (MQ=255)

                                                 gACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACAt                                                                                                               >  6:212317/1‑129 (MQ=255)

                                                    ccGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGa                                                                                        <  6:140627/149‑1 (MQ=255)

                                                     cGGTGGCCGAGTGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGAt                                                                                       <  3:312486/149‑1 (MQ=255)

                                                        tGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATc                                                                                      <  7:294726/147‑1 (MQ=255)

                                                            cGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTc                                                                                <  2:32754/149‑1 (MQ=255)

                                                                ggCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAgg                                                                            <  4:230105/149‑1 (MQ=255)

                                                                 gCACGCTGCTGGGGATCAAGGGCCAGTACCTGATCTTCGACACCTGTGTTATCAATATTCGCAATTACACGGCCTACCAGTTGTCC‑GTGCTCCAGTAAAAAGGACCTTCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAgg                                                                            <  3:168560/148‑1 (MQ=255)

                                                                 gCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGa                                                                           <  6:99238/149‑1 (MQ=255)

                                                                 gCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGa                                                                           <  3:92892/149‑1 (MQ=255)

                                                                     gctgctGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTAc                                                                       >  3:2708/1‑149 (MQ=255)

                                                                     gctgctGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTAc                                                                       >  7:212097/1‑149 (MQ=255)

                                                                         ctgGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAg                                                                    <  4:2708/148‑1 (MQ=255)

                                                                            ggCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGgc                                                                  <  1:14089/147‑1 (MQ=255)

                                                                              cATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCg                                                              >  4:244587/1‑149 (MQ=255)

                                                                               aTCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTAGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCg                                                              >  8:250478/1‑148 (MQ=255)

                                                                                 cAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAg                                                           >  3:33220/1‑149 (MQ=255)

                                                                                    gggCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTAc                                                        <  3:244587/149‑1 (MQ=255)

                                                                                       ccAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATg                                                                                                              >  1:202485/1‑92 (MQ=255)

                                                                                       ccAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATg                                                                                                              <  2:202485/92‑1 (MQ=255)

                                                                                       ccAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTAcat                                                      >  6:105404/1‑146 (MQ=255)

                                                                                       ccAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTg                                                     <  3:69584/149‑1 (MQ=255)

                                                                                       ccAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACAGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCt                                                      >  6:228454/1‑148 (MQ=255)

                                                                                        cAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTg                                                     <  5:228454/148‑1 (MQ=255)

                                                                                        cAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGa                                                    <  4:33220/149‑1 (MQ=255)

                                                                                         aGTTCCTGATCTTCGACACCGGTGTGATCAATATTCGCATGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAATGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGAt                                                   <  3:172918/149‑1 (MQ=255)

                                                                                             ccTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgac                                               <  4:126422/149‑1 (MQ=255)

                                                                                              cTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATCCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgac                                               >  1:262887/1‑148 (MQ=255)

                                                                                                gATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATcgac                                               >  4:276547/1‑146 (MQ=255)

                                                                                                 aTCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACgaga                                           >  2:61217/1‑149 (MQ=255)

                                                                                                  tCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGAc                                                                                                                       >  6:53890/1‑72 (MQ=255)

                                                                                                  tCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGAc                                                                                                                       <  5:53890/72‑1 (MQ=255)

                                                                                                  tCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGAc                                          >  6:142130/1‑149 (MQ=255)

                                                                                                          acCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGa                                  <  7:250478/149‑1 (MQ=255)

                                                                                                               tgtgATCAATATTCTCAAGTACACGGCCTACCAGTTGGGC‑GTGCTCCAGTAAAAAGGACCTGCTCATGCGTACCGAACAACCGCAAGTGATGTACCTCAAGGATTACCAGGCGCCGGAGTACGTGATCGACGTGACGCAGCTGACCTTc                             <  8:53274/149‑1 (MQ=255)

                                                                                                               tgtgATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCaa                                                                                            >  1:114873/1‑86 (MQ=255)

                                                                                                               tgtgATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCaa                                                                                            <  2:114873/86‑1 (MQ=255)

                                                                                                                   aTCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGc                         >  7:72838/1‑149 (MQ=255)

                                                                                                                     cAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTCCCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTg                       >  2:51009/1‑149 (MQ=255)

                                                                                                                      aaTATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGt                      <  6:75483/149‑1 (MQ=255)

                                                                                                                        tatTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTc                    <  5:285078/149‑1 (MQ=255)

                                                                                                                        tatTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTc                    <  5:142130/149‑1 (MQ=255)

                                                                                                                          ttCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTc                    >  3:297713/1‑147 (MQ=255)

                                                                                                                           tCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCTAGCTGTTCGAg                 >  3:222389/1‑149 (MQ=255)

                                                                                                                             gCAAGTACACGGCCTACCAGTTGGCCGGTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAg                 >  1:4214/1‑148 (MQ=255)

                                                                                                                             gCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTg                       >  1:310683/1‑141 (MQ=255)

                                                                                                                             gCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTg                       <  2:310683/141‑1 (MQ=255)

                                                                                                                             gCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGa               >  2:227135/1‑149 (MQ=255)

                                                                                                                              cAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGAc              >  6:44156/1‑149 (MQ=255)

                                                                                                                               tggagcacGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGa                                                            <  4:281080/97‑1 (MQ=255)

                                                                                                                               tggagcacGGCCAACTGGTAGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGAc              >  4:79981/6‑148 (MQ=255)

                                                                                                                               aaGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGAcc             <  8:212097/149‑1 (MQ=255)

                                                                                                                                  tACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACcaca          >  3:304699/1‑149 (MQ=255)

                                                                                                                                     acGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACgaga                                           >  6:138276/1‑113 (MQ=255)

                                                                                                                                     acGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACgaga                                           <  5:138276/113‑1 (MQ=255)

                                                                                                                                     acGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGc       <  5:105404/149‑1 (MQ=255)

                                                                                                                                     acGGCCAACTGGTAGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGc       >  1:211871/1‑149 (MQ=255)

                                                                                                                                       ggCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAgg                                                                   >  7:170123/1‑87 (MQ=255)

                                                                                                                                       ggCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTg     <  1:61217/149‑1 (MQ=255)

                                                                                                                                       ggCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTg     >  1:31063/1‑149 (MQ=255)

                                                                                                                                        gacTACCAGTTGGCC‑GTTCTCCAGTAAAAAGGACCTTCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCATGCTCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTgg    <  4:222389/147‑1 (MQ=255)

                                                                                                                                          cTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGtt  >  8:229116/1‑149 (MQ=255)

                                                                                                                                           tACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGtt  >  4:127656/1‑148 (MQ=255)

                                                                                                                                           tACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGtt  >  8:32370/1‑148 (MQ=255)

                                                                                                                                             ccAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTc                    >  8:281192/1‑128 (MQ=255)

                                                                                                                                             ccAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTc                    <  7:281192/128‑1 (MQ=255)

                                                                                                                                                gTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTg     >  6:73540/1‑140 (MQ=255)

                                                                                                                                                gTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTg     <  5:73540/140‑1 (MQ=255)

CCCGGTCGAGGCGTACCCGAAGAAGATTGTCAGCTTCAACCTCGACAAGGACCCGGTGGCCGAGGGCACGCTGCTGGGCATCAAGGGCCAGTACCTGATCTTCGACACCGGTGTGATCAATATTCGCAAGTACACGGCCTACCAGTTGGCC‑GTGCTCCAGTAAAAAGGACCTGCACATGCGTACCGAACAACCGCAAGTGATCTACCTCAAGGATTACCAGGCGCCGGAGTACCTGATCGACGAGACGCACCTGACCTTCGAGCTGTTCGAGGACCACACGCTGGTT  >  NC_002947/2287542‑2287828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`