Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,297,142	T→A	7.3%	T994S (ACC→TCC)	sbcC ←	exonuclease subunit SbcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,297,142	0	T	A	7.3%	228.8 / 7.8	83	T994S (ACC→TCC)	sbcC	exonuclease subunit SbcC
Reads supporting (aligned to +/- strand):  ref base T (36/41);  new base A (3/3);  total (39/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.09e-01

TGTTCGATTTCTGCCGCCAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCCAGCAGGCGATC  >  NC_002947/2296994‑2297289                                                                                                                                                     |                                                                                                                                                    tGTTCGATTTCTGCCGCCAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCgtggt                                                                                                                                                     >  1:151449/1‑149 (MQ=255) tGTTCGATTTCTGCCGCCAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCgtggt                                                                                                                                                     >  4:177191/1‑149 (MQ=255) tGTTCGATTTCTGCCGCCAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCgtggt                                                                                                                                                     >  2:134937/1‑149 (MQ=255)   ttCGATTTCTGCCGCCAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGgtgt                                                                                                                                                   >  4:139006/1‑149 (MQ=255)          tCTGCCGCCAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACgg                                                                                                                                             <  2:303037/148‑1 (MQ=255)            tGCCGCCAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCTGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCGACGGTCa                                                                                                                                          >  4:24775/1‑149 (MQ=255)              ccgccAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATc                                                                                                                                        <  3:95770/149‑1 (MQ=255)              ccgccAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATc                                                                                                                                        <  2:151449/149‑1 (MQ=255)              ccgccAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGACACCACGGTcc                                                                                                                                          <  5:207551/147‑2 (MQ=255)              ccgccAGCGCCTGGTGGGCCTGCGGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGACACCACGGTccaa                                                                                                                                        <  8:303453/149‑4 (MQ=255)                gccAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCtg                                                                                                                                      >  5:309607/1‑149 (MQ=255)                      gcCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCcgc                                                                                                                                >  3:1134/1‑149 (MQ=255)                      gcCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCcgc                                                                                                                                <  7:261230/149‑1 (MQ=255)                      gcCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCcgc                                                                                                                                <  2:154965/149‑1 (MQ=255)                        cTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTATGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGt                                                                                                                              <  1:104456/149‑1 (MQ=255)                           gtggGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGAt                                                                                                                           <  2:317000/149‑1 (MQ=255)                             gggCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGAtgc                                                                                                                         >  1:298429/1‑149 (MQ=255)                              ggCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGAGTCCACGGTCATCTGTGCCGCGTGAtgc                                                                                                                         <  6:146680/148‑1 (MQ=255)                               gCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGAtgctg                                                                                                                       >  3:25614/1‑149 (MQ=255)                                ccTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCATGGTCATCTGTGCCGCGTGAtgctgc                                                                                                                      <  4:321751/149‑1 (MQ=255)                                ccTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGAtgctg                                                                                                                       >  8:293799/1‑148 (MQ=255)                                ccTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGAtgctg                                                                                                                       >  3:118712/1‑148 (MQ=255)                                    ctgGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAg                                                                                                                    <  5:53330/147‑1 (MQ=255)                                     tgGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGgcg                                                                                                                 >  4:25940/1‑149 (MQ=255)                                          gcgcTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGtt                                                                                                            <  3:139006/149‑1 (MQ=255)                                          gcgcTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGt                                                                                                             >  7:286187/1‑148 (MQ=255)                                           cgcTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTc                                                                                                           <  4:1134/149‑1 (MQ=255)                                                gtcgtcGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCgcgc                                                                                                       <  5:159239/148‑1 (MQ=255)                                                gtcgtcGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCgcgc                                                                                                       <  3:177191/148‑1 (MQ=255)                                                      ggCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTgc                                                                                                 <  3:25940/148‑1 (MQ=255)                                                          tgttgtGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTcg                                                                                                          >  4:85705/1‑135 (MQ=255)                                                          tgttgtGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTcg                                                                                                          <  3:85705/135‑1 (MQ=255)                                                           gttgtGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGcagcag                                                                                            >  8:227416/1‑148 (MQ=255)                                                            ttgtGCACGCAATTCGGCACATTGCTGCTCGTGGTTTGCCGGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGcagcag                                                                                            <  8:252037/147‑1 (MQ=255)                                                               tgCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGAcgc                                                                                       >  6:43620/1‑149 (MQ=255)                                                                 cACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCgg                                                                                     <  3:91724/149‑1 (MQ=255)                                                                 cACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCgg                                                                                     <  1:175356/149‑1 (MQ=255)                                                                  aCGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGGCCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGc                                                                                    >  3:300155/1‑149 (MQ=255)                                                                  aCGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGc                                                                                    <  6:153215/149‑1 (MQ=255)                                                                   cGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGcc                                                                                   >  8:135078/1‑149 (MQ=255)                                                                        ttCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCt                                                                              >  4:29742/1‑149 (MQ=255)                                                                        ttCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCt                                                                              >  3:207150/1‑149 (MQ=255)                                                                             cgcATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATg                                                                         <  7:293799/149‑1 (MQ=255)                                                                             cgcATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCACCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATg                                                                         <  1:269932/149‑1 (MQ=255)                                                                                aTTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCt                                                                      >  1:84737/1‑149 (MQ=255)                                                                                    ctgctCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGATGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCttttt                                                                  >  8:204292/1‑149 (MQ=255)                                                                                       ctCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTcc                                                                <  7:227416/148‑1 (MQ=255)                                                                                        tCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCcgc                                                              >  8:4292/1‑149 (MQ=255)                                                                                        tCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCcgc                                                              >  3:194241/1‑149 (MQ=255)                                                                                           tGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGcc                                                           >  3:170111/1‑149 (MQ=255)                                                                                             gTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATTCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCtt                                                         <  5:43620/149‑1 (MQ=255)                                                                                                  ccAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGAGCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCaaa                                                    >  1:231838/1‑149 (MQ=255)                                                                                                  ccAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTTATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATCGCTTTTTCCGCGCCTTGCaaa                                                    <  4:25614/149‑1 (MQ=255)                                                                                                   cAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAAc                                                   >  2:112731/1‑149 (MQ=255)                                                                                                   cAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAAc                                                   >  1:96338/1‑149 (MQ=255)                                                                                                       cGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTg                                               <  8:286187/149‑1 (MQ=255)                                                                                                        gTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTgg                                              <  4:170111/149‑1 (MQ=255)                                                                                                        gTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTgg                                              >  8:134564/1‑149 (MQ=255)                                                                                                         ttCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGgc                                             <  4:207150/149‑1 (MQ=255)                                                                                                              gcAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAAt                                        >  1:245897/1‑149 (MQ=255)                                                                                                              gcAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAAt                                        >  4:301934/1‑149 (MQ=255)                                                                                                               cAGGTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAAtt                                       <  6:303198/149‑1 (MQ=255)                                                                                                                aGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTc                                      <  2:84737/149‑1 (MQ=255)                                                                                                                 gTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCa                                     <  4:118712/149‑1 (MQ=255)                                                                                                                 gTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCa                                     <  1:112731/149‑1 (MQ=255)                                                                                                                 gTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCa                                     <  5:320304/149‑1 (MQ=255)                                                                                                                    cGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCAtt                                   <  7:134564/148‑1 (MQ=255)                                                                                                                    cGGCCAAGCCTTGCTCCAGGGCTTGGACCGGGGTGTCCACGGTCAGCTGTGCCGCGTGATGCTGCACGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCAtt                                   <  4:57591/148‑1 (MQ=255)                                                                                                                     ggCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGa                                 <  5:329411/149‑1 (MQ=255)                                                                                                                      gCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGAc                                >  8:126100/1‑149 (MQ=255)                                                                                                                       ccAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACt                               >  2:151776/1‑149 (MQ=255)                                                                                                                        cAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACtt                              >  7:251927/1‑149 (MQ=255)                                                                                                                              ttGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGcgcg                         >  6:300551/1‑148 (MQ=255)                                                                                                                                 cTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCa                      <  2:96338/148‑1 (MQ=255)                                                                                                                                 cTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCAt                     <  2:298429/149‑1 (MQ=255)                                                                                                                                 cTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAAGTCAATGAATTgcgccagc                      <  4:194241/148‑5 (MQ=255)                                                                                                                                  tCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATc                    >  7:21020/1‑149 (MQ=255)                                                                                                                                  tCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATc                    >  4:40054/1‑149 (MQ=255)                                                                                                                                    cAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCa                  >  2:165383/1‑149 (MQ=255)                                                                                                                                    cAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCa                  >  6:13564/1‑149 (MQ=255)                                                                                                                                     aGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCAt                 <  3:40054/149‑1 (MQ=255)                                                                                                                                     aGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCAt                 >  8:11813/1‑149 (MQ=255)                                                                                                                                      gggCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATg                <  2:231838/149‑1 (MQ=255)                                                                                                                                          gatgACCGTGGACACCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGc              >  6:105123/4‑147 (MQ=255)                                                                                                                                             gACCGTGGACACCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCcag           >  7:267185/1‑147 (MQ=255)                                                                                                                                             gACCGTGGACACCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCcag           >  2:100818/1‑147 (MQ=255)                                                                                                                                               ccGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCCAGCAgg       <  1:134937/149‑1 (MQ=255)                                                                                                                                                    gtgtCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCCAGCAGGCGATc  >  4:227638/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TGTTCGATTTCTGCCGCCAGCGCCTGGTGGGCCTGCTGGCGGCGCTGGTCGTCGGCCTGTTGTGCACGCAATTCGGCGCATTGCTGCTCGTGGTTTGCCAGGCGTTCGCGCAGTTCGGCCAAGGCTTGCTCCAGGGCTTGGACCGTGGTGTCCACGGTCATCTGTGCCGCGTGATGCTGCAGGCGCTGTTCGCGCTCCTGCAGCAGGACGCGGCCCTGCTCGATGGCTTTTTCCGCGCCTTGCAAACGCTGGCGCAATTCATTGACTTGCGCGTCATCCATGGCCAGCAGGCGATC  >  NC_002947/2296994‑2297289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑