Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	235,742	T→C	5.9%	M365V (ATG→GTG)	argH ←	argininosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	235,742	0	T	C	5.9%	201.7 / 2.3	68	M365V (ATG→GTG)	argH	argininosuccinate lyase
Reads supporting (aligned to +/- strand):  ref base T (36/28);  new base C (2/2);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

TCTCGGCCAGGTCCTTGCCAGTGTCCACACCATACTTCACCGCGTGGCCAACGATCTCGTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCTTGTTGTAGGCCAGCGGTTGGCCTTTCATCAGGGTCAG  >  NC_002947/235595‑235889                                                                                                                                                    |                                                                                                                                                    tctcGGCCAGGTCCTTGCCAGTGTCCACACCATACTTCACCGCGTGGCCAACGATCTCGTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCatg                                                                                                                                                    >  7:150357/1‑149 (MQ=255)        cAGGTCCTTGCCAGAGTCCACACCATACTTCACCGCGTGGCCAACGATCTCGTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGACACCGCGCAGGGCCGCTTCACGCATGATGGCGt                                                                                                                                             >  7:276602/1‑149 (MQ=255)                 gCCAGTGTCCACACCATACTTCACCGCGTGGCCAACGATCTCGTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGc                                                                                                                                     <  1:198929/148‑1 (MQ=255)                  ccAGTGTCCACACCATACTTCACCGCGTGGCCAACGATCTCGTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCtt                                                                                                                                   <  2:208453/149‑1 (MQ=255)                     gtgtCCACACCATACTTCACCGCGTGGCCAACGATCTCGTGGCAGTCACGGAACGGCAGGCCCCGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGAt                                                                                                                                >  1:271852/1‑149 (MQ=255)                        tCCACACCATACTTCACCGCGTGGCCAACGATCTCGTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGc                                                                                                                             >  1:24646/1‑149 (MQ=255)                           acacCATACTTCACCGCGTGGCCAACGATCTCGTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCatgatg                                                                                                                                                 >  1:58404/1‑126 (MQ=255)                           acacCATACTTCACCGCGTGGCCAACGATCTCGTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCatgatg                                                                                                                                                 <  2:58404/126‑1 (MQ=255)                             acCATACTTCACCGCGTGGCCAACGATCTCGTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTg                                                                                                                                        >  8:289433/1‑133 (MQ=255)                             acCATACTTCACCGCGTGGCCAACGATCTCGTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTg                                                                                                                                        <  7:289433/133‑1 (MQ=255)                                    ttCACCGCGTGGCCAACGATCTCGTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGt                                                                                                                  >  3:289227/1‑148 (MQ=255)                                      cACCGCGTGGCCAACGATCTCGTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAg                                                                                                               >  4:95047/1‑149 (MQ=255)                                       aCCGCGTGGCCAACGATCTCGTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCg                                                                                                                           <  8:62733/136‑1 (MQ=255)                                       aCCGCGTGGCCAACGATCTCGTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCg                                                                                                                           >  7:62733/1‑136 (MQ=255)                                          gcgTGGCCAACGATCTCGTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCg                                                                                                             >  7:278008/1‑147 (MQ=255)                                                ccAACGATCTCGTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCg                                                                                                     >  2:298048/1‑149 (MQ=255)                                                ccAACGATCTCGTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCg                                                                                                     >  2:164054/1‑149 (MQ=255)                                                 cAACGATCTCGTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGc                                                                                                    >  8:163404/1‑149 (MQ=255)                                                   aCGATCTCGTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAg                                                                                                  >  5:67386/1‑149 (MQ=255)                                                           gTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGa                                                                                               <  3:95047/144‑1 (MQ=255)                                                           gTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAg                                                                                              <  6:67386/145‑1 (MQ=255)                                                           gTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTc                                                                                            <  5:207989/147‑1 (MQ=255)                                                           gTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTc                                                                                            <  4:289227/147‑1 (MQ=255)                                                              gCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCa                                                                                       <  8:150357/149‑1 (MQ=255)                                                              gCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCa                                                                                       <  3:136838/149‑1 (MQ=255)                                                                aGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAgg                                                                                     >  3:295374/1‑149 (MQ=255)                                                                  tCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTc                                                                                            <  1:253049/140‑1 (MQ=255)                                                                  tCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTc                                                                                            >  2:253049/1‑140 (MQ=255)                                                                  tCACGGAACGGCAGGCCACGCCGCACCAGATAGTAAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTAACGCATGATGGCGGGCTTGGGGTTGATCGCCGGGAGCAGGTCAGCGAAGGCCCCCCGCGAGTCGCGCAggg                                                                                    >  6:262479/1‑148 (MQ=255)                                                                      ggAACGGCAGGCTACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATcg                                                                               >  5:291613/1‑149 (MQ=255)                                                                      ggAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATcg                                                                               >  1:307053/1‑149 (MQ=255)                                                                      ggAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATcg                                                                               >  4:125790/1‑149 (MQ=255)                                                                        aaCGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATcggc                                                                             >  6:288001/1‑149 (MQ=255)                                                                           ggCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGa                                                                                               <  3:194762/128‑1 (MQ=255)                                                                           ggCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGa                                                                                               >  4:194762/1‑128 (MQ=255)                                                                           ggCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCggcggc                                                                          <  7:163404/149‑1 (MQ=255)                                                                            gCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACTCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGgcggcg                                                                         <  2:307053/149‑1 (MQ=255)                                                                            gCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGgcggcg                                                                         >  3:75235/1‑149 (MQ=255)                                                                             cAGGCCACGGCTAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCatgatg                                                                                                                                                 <  5:248199/76‑1 (MQ=255)                                                                              aGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTc                                                                       >  7:192610/1‑149 (MQ=255)                                                                                 ccACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGCCCCGCAGCGAGTCTCGCAGGGTATCGGCGGCGTCGaa                                                                    >  2:107835/1‑149 (MQ=255)                                                                                     ggCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCCGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGc                                                                >  5:239943/1‑149 (MQ=255)                                                                                     ggCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGc                                                                >  1:316769/1‑149 (MQ=255)                                                                                          aCCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTc                                                           >  3:196630/1‑149 (MQ=255)                                                                                           ccAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTcc                                                          <  5:288001/149‑1 (MQ=255)                                                                                              gATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTg                                                       <  1:164054/149‑1 (MQ=255)                                                                                               aTAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGt                                                      >  5:294986/1‑149 (MQ=255)                                                                                                 aGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCt                                                    >  7:273690/1‑149 (MQ=255)                                                                                                     aGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCt                                                >  6:241462/1‑149 (MQ=255)                                                                                                        cAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGt                                             <  6:291613/149‑1 (MQ=255)                                                                                                                  gCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCttgtt                                   <  1:298048/149‑1 (MQ=255)                                                                                                                     gTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGtt                                            >  2:171119/1‑137 (MQ=255)                                                                                                                     gTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGtt                                            <  1:171119/137‑1 (MQ=255)                                                                                                                       ggAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCTTGTTGTAgg                              <  2:271852/149‑1 (MQ=255)                                                                                                                        gagaAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCTTGTTGTAGGc                             >  5:10119/1‑149 (MQ=255)                                                                                                                         agaAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCTTGTTGTAGGc                             >  6:8977/1‑148 (MQ=255)                                                                                                                           aaaCCGCGCAGGGCCGCTTCACGCACGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGtt                                            <  3:142852/131‑1 (MQ=255)                                                                                                                           aaaCCGCGCAGGGCCGCTTCACGCACGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGtt                                            >  4:142852/1‑131 (MQ=255)                                                                                                                           aaaCCGCGCAGGGCCGCTTCACGCACGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGtt                                            <  5:203892/131‑1 (MQ=255)                                                                                                                           aaaCCGCGCAGGGCCGCTTCACGCACGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGtt                                            >  6:203892/1‑131 (MQ=255)                                                                                                                             aCCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCTTGTTGTAGGCCAGCg                        >  5:54864/1‑149 (MQ=255)                                                                                                                              ccGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGCATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCTTGTTGTAGGCCAGCgg                       >  7:34361/1‑149 (MQ=255)                                                                                                                                 cgcAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCTTGTTGTAGGCCAGCGGTTg                    <  4:75235/149‑1 (MQ=255)                                                                                                                                  gcAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCTTGTTGTAGGCCAGCGGTTgg                   <  4:196630/149‑1 (MQ=255)                                                                                                                                   cAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCTTGTTGTAGGCCAGCGGTTGGc                  <  5:241462/149‑1 (MQ=255)                                                                                                                                      ggCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCTTGTTGTAGGCCAGCGGTTGGCCtt               >  4:112318/1‑149 (MQ=255)                                                                                                                                             tCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCttgt                                    >  6:175344/1‑121 (MQ=255)                                                                                                                                             tCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCttgt                                    <  5:175344/121‑1 (MQ=255)                                                                                                                                              cACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCTTGTTGTAGGCCAGCGGTTGGCCTTccatca         <  4:295374/147‑1 (MQ=255)                                                                                                                                               aCGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCTTGTTGTAGGCCAGCGGTTGGCCTTTCATCAgg       <  2:316769/148‑1 (MQ=255)                                                                                                                                                cGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCTTGTTGTAGGCCAGCGGTTGGCCTTTCATCAGGGt     <  6:239943/149‑1 (MQ=255)                                                                                                                                                cGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCTTGTTGTAGGCCAGCGGTTGGCCTTTCATCAGGGt     <  2:24646/149‑1 (MQ=255)                                                                                                                                                   atgatgGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCTTGTTGTAGGCCAGCGGTTGGCCTTTCATCAGGGt     >  7:227731/1‑146 (MQ=255)                                                                                                                                                   atgatgGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCTTGTTGTAGGCCAGCGGTTGGCCTTTCATCAGGGt     <  8:227731/146‑1 (MQ=255)                                                                                                                                                   atgatgGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCACAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCTTGTTGTAGGCCAGCGGTTGGCCTTTCACCAGGGTcag  >  7:250755/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TCTCGGCCAGGTCCTTGCCAGTGTCCACACCATACTTCACCGCGTGGCCAACGATCTCGTGGCAGTCACGGAACGGCAGGCCACGGCGAACCAGATAGTCAGCCAGGTCGGTAGCGGTGGAGAAACCGCGCAGGGCCGCTTCACGCATGATGGCGTGCTTGGGCTTGATCGCCGGGATCATGTCAGCGAAGGCCCGCAGCGAGTCGCGCAGGGTATCGGCGGCGTCGAACAGCGGTTCCTTGTCTTCCTGGTTGTCCTTGTTGTAGGCCAGCGGTTGGCCTTTCATCAGGGTCAG  >  NC_002947/235595‑235889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑