BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,298,604	G→A	5.2%	D506D (GAC→GAT)	sbcC ←	exonuclease subunit SbcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,298,604	0	G	A	5.2%	300.2 / 3.2	96	D506D (GAC→GAT)	sbcC	exonuclease subunit SbcC
Reads supporting (aligned to +/- strand): ref base G (46/45); new base A (3/2); total (49/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.08e-01

GCGTTGCAGCATGGCCTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTGGTCAGCC  >  NC_002947/2298463‑2298749

gCGTTGCAGCATGGCCTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGAt                                                                                                                                               <  3:247621/147‑1 (MQ=255)

gCGTTGCAGCATGGCCTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCt                                                                                                                                             >  2:251215/1‑149 (MQ=255)

gCGTTGCAGCATGGCCTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCt                                                                                                                                             <  6:211797/149‑1 (MQ=255)

  gTTGCAGCATGGCCTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGc                                                                                                                                           >  5:298745/1‑149 (MQ=255)

      cagcaTGGCCTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGATCGACCTGCTCgg                                                                                                                                       <  3:2390/149‑1 (MQ=255)

       agcaTGGCCTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGc                                                                                                                                      <  4:36020/149‑1 (MQ=255)

          aTGGCCTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAg                                                                                                                                   >  4:169377/1‑149 (MQ=255)

             gCCTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGc                                                                                                                                >  5:327067/1‑149 (MQ=255)

                  ctgctgcCGTTCTCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGGTGTCCTTCATCATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTg                                                                                                                             <  6:262710/147‑1 (MQ=255)

                  ctgctgcCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTggg                                                                                                                           <  6:219462/149‑1 (MQ=255)

                   tgctgcCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTc                                                                                                                                         <  4:212652/134‑1 (MQ=255)

                   tgctgcCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTc                                                                                                                                         >  3:212652/1‑134 (MQ=255)

                     ctgcCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGtt                                                                                                                        <  3:169377/149‑1 (MQ=255)

                      tgcCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTc                                                                                                                       >  3:196547/1‑149 (MQ=255)

                      tgcCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTACAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTc                                                                                                                       >  6:64383/1‑149 (MQ=255)

                        ccGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCgg                                                                                                                     >  4:300882/1‑149 (MQ=255)

                        ccGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCg                                                                                                                      <  6:307274/148‑1 (MQ=255)

                          gTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGcc                                                                                                                   >  3:77400/1‑149 (MQ=255)

                          gTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTAACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATTCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGcc                                                                                                                   >  3:283661/1‑149 (MQ=255)

                              gcgcAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTgg                                                                                                               <  6:52334/149‑1 (MQ=255)

                               cgcAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTACAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGc                                                                                                              <  5:64383/149‑1 (MQ=255)

                                 cAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCtt                                                                                                            <  6:298745/149‑1 (MQ=255)

                                  aGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCtt                                                                                                            >  3:281964/1‑148 (MQ=255)

                                  aGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCtt                                                                                                            >  3:286595/1‑148 (MQ=255)

                                        cccAGCTGCCGGCGCAGCGCCGGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGCTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGaa                                                                                                     <  6:95821/148‑1 (MQ=255)

                                              tgctgGTGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAg                                                                                               >  8:107516/1‑149 (MQ=255)

                                                ctgGCGCAGGTCCTGTTCACGGCCTTGCAGGCGTGACATTTCCTCTACGGCGCGATGCTGTTTGCGGTTGTCATGCAACATGCCGCCGTGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAgg                                                                                              <  4:283661/148‑1 (MQ=255)

                                                ctgGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑t                                                                                            >  4:14871/1‑149 (MQ=255)

                                                    cgcAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTa                                                                                        <  4:71093/149‑1 (MQ=255)

                                                       aGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGc                                                                                     >  5:104226/1‑149 (MQ=255)

                                                          tCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGaacaac                                                                                                 <  7:26180/135‑1 (MQ=255)

                                                          tCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGaacaac                                                                                                 >  8:26180/1‑135 (MQ=255)

                                                          tCCTGTACACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGt                                                                                         <  3:95460/142‑1 (MQ=255)

                                                          tCCTGTACACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGt                                                                                         >  4:95460/1‑142 (MQ=255)

                                                            cTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTcgc                                                                                >  4:48248/1‑149 (MQ=255)

                                                             tGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGaa                                                                                                     <  6:211550/128‑1 (MQ=255)

                                                             tGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGaa                                                                                                     >  5:211550/1‑128 (MQ=255)

                                                                  aCGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTg                                                                                                                             <  7:26424/99‑1 (MQ=255)

                                                                  aCGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTg                                                                                                                             >  8:26424/1‑99 (MQ=255)

                                                                   cGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGc                                                                         <  6:104226/149‑1 (MQ=255)

                                                                      ccGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAgg                                                                                                                                  <  1:323683/90‑1 (MQ=255)

                                                                      ccGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAgg                                                                                                                                  >  2:323683/1‑90 (MQ=255)

                                                                       cGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGc                                                                     <  4:77400/149‑1 (MQ=255)

                                                                       cGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGCCGTCGCGCTCGGCCTg                                                                      >  2:232795/1‑148 (MQ=255)

                                                                                gTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAgc                                                                 <  7:91686/144‑1 (MQ=255)

                                                                                gTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAgc                                                                 >  8:91686/1‑144 (MQ=255)

                                                                                   aCATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTg                                                         >  5:219437/1‑149 (MQ=255)

                                                                                   aCATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTg                                                         >  3:179021/1‑149 (MQ=255)

                                                                                    cATTTCCTCTACGGCGCGCTGCTGTTTGCCGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGTCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTg                                                         <  4:196547/148‑1 (MQ=255)

                                                                                         ccTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCctg                                                    <  3:14871/148‑1 (MQ=255)

                                                                                           tctACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCctggct                                                 >  7:42144/1‑149 (MQ=255)

                                                                                              aCGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCtggctggc                                              >  7:200796/1‑149 (MQ=255)

                                                                                               cGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTggcc                                             >  6:108957/1‑149 (MQ=255)

                                                                                                  cgcgcTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTggcctgg                                          <  3:48248/149‑1 (MQ=255)

                                                                                                     gctgctGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGGCTCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTggcctgggc                                        <  1:251215/149‑1 (MQ=255)

                                                                                                     gctgctGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTggcctgggcc                                       >  7:120909/1‑149 (MQ=255)

                                                                                                      ctgctgTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTggcctgggcct                                      >  6:85078/1‑149 (MQ=255)

                                                                                                       tgctgTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTggcctgggcctg                                     >  4:116987/1‑149 (MQ=255)

                                                                                                        gctgTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTggcctgggcctg                                     >  6:287819/1‑148 (MQ=255)

                                                                                                                cGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGTTCTGCGCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCggg                            <  3:301530/149‑1 (MQ=255)

                                                                                                                cGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCgg                             <  7:159550/148‑1 (MQ=255)

                                                                                                                 ggTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCTTGGGCCTGCAGGCCggg                            >  6:267447/1‑148 (MQ=255)

                                                                                                                 ggTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGt                           <  6:219437/149‑1 (MQ=255)

                                                                                                                 ggTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGt                           <  4:320265/149‑1 (MQ=255)

                                                                                                                  gTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTa                          <  5:74493/149‑1 (MQ=255)

                                                                                                                   ttGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAg                         <  4:286595/149‑1 (MQ=255)

                                                                                                                   ttGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAg                         <  4:281964/149‑1 (MQ=255)

                                                                                                                   ttGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAg                         <  4:151138/149‑1 (MQ=255)

                                                                                                                     gTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGct                       >  3:52659/1‑149 (MQ=255)

                                                                                                                      tCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGctc                      >  5:16006/1‑149 (MQ=255)

                                                                                                                        cTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGctct                     <  8:42144/148‑1 (MQ=255)

                                                                                                                        cTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGctct                     >  4:69184/1‑148 (MQ=255)

                                                                                                                        cTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCtctt                    >  6:184726/1‑149 (MQ=255)

                                                                                                                         tGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGccaatgc                          <  3:111098/142‑6 (MQ=255)

                                                                                                                         tGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGccaatgc                          >  4:111098/1‑137 (MQ=255)

                                                                                                                         tGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCtcttc                   >  1:47373/1‑149 (MQ=255)

                                                                                                                         tGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCtcttc                   >  2:278764/1‑149 (MQ=255)

                                                                                                                         tGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCtcttc                   >  8:286727/1‑149 (MQ=255)

                                                                                                                           cAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCtcttct                  <  5:85078/148‑1 (MQ=255)

                                                                                                                            aaCATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCg                >  5:247845/1‑149 (MQ=255)

                                                                                                                             aCATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCgg               <  5:108957/149‑1 (MQ=255)

                                                                                                                              cATGCCGCCGCGCAGGTCGATCTGCTCGGCCAGGGCCGGGGGTTCGGCCTGGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCCTTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTGCTCGGc              >  7:122165/1‑149 (MQ=255)

                                                                                                                               aTGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAg                         >  2:47413/1‑137 (MQ=255)

                                                                                                                               aTGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAg                         <  1:47413/137‑1 (MQ=255)

                                                                                                                                 ctggccgAGCAGATCGACCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCt           >  1:120138/4‑149 (MQ=255)

                                                                                                                                  ccgccgAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCtt          <  3:300882/149‑1 (MQ=255)

                                                                                                                                  ccgccgAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCtt          >  1:305547/1‑149 (MQ=255)

                                                                                                                                   cgccgAGCAGGTCGATCTGCTCGGCGAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGGCGAGCTCGTACTTGAATCGCGCATTGGCCTGTCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCACTTg         <  5:228254/149‑1 (MQ=255)

                                                                                                                                   cgccgAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTg         <  2:22306/149‑1 (MQ=255)

                                                                                                                                     ccgAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTGGt       <  4:133870/149‑1 (MQ=255)

                                                                                                                                     ccgAGCAGATCGACCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTGGt       >  8:30475/1‑149 (MQ=255)

                                                                                                                                      cgAGCAGATCGACCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTtctc                 >  2:217345/1‑138 (MQ=255)

                                                                                                                                      cgAGCAGATCGACCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTtctc                 <  1:217345/138‑1 (MQ=255)

                                                                                                                                       gAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTGGTCa     <  3:69184/149‑1 (MQ=255)

                                                                                                                                        agcagcTCGATCTGCTCGGCCAGGGCCAGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTGGTCAg    >  5:66661/1‑149 (MQ=255)

                                                                                                                                         gCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTGGTCAGc   >  1:84801/1‑149 (MQ=255)

                                                                                                                                          cAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTGGTCAGcc  <  2:47373/149‑1 (MQ=255)

GCGTTGCAGCATGGCCTGCTGCTGCCGTTCGCGCAGGGCATCCAGCTGCTGGCGCAGCTCCTGTTCACGGCCGTGCAGGCGTGACATTTCCTCTACGGCGCGCTGCTGTTTGCGGTTGTCCTGCAACATGCCGCCGAGCAGGTCGATCTGCTCGGCCAGGGCCTGGGGTTCGGCCTTGGCTTCACGGAACAACAGG‑TCGTAGGCGTCGCGCTCGGCCTGCAAGCGCGCATTGGCCTGGCTGGCCTGGGCCTGCAGGCCGGGTAGCTCTTCTCGGCCCTTGGTCAGCC  >  NC_002947/2298463‑2298749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`