Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 266,851 G→A 8.7% V187V (GTG→GTA)  PP_0215 → two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947266,8510GA 8.7% 128.6 / 3.3 46V187V (GTG→GTA) PP_0215two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base G (22/20);  new base A (2/2);  total (24/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GTTGCAGGCGGGGGCCACCCTGTCAGCCTACCTTGAGCAGCGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTGCCGCCAATAGTGCGGCATTGCACCGTGAAGCCCCGGTGCAGACGTTGCTGCAGGCCCTGAACAAGCTTGGCAGTACCCAAAGCATGAACCTGATCCTGGGCATGACC  >  NC_002947/266704‑266995
                                                                                                                                                   |                                                                                                                                                
gTTGCAGGCGGGGGCCACCCTGTCAGCCTACCTTGAGCAGCGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGc                                                                                                                                                 <  8:109607/149‑1 (MQ=255)
 ttGCAGGCGGGGGCCACCCTGTCAGCCTACATTGAGCAGCGTCGCGCTACCGCCGATGGTGGGCCGCTGATGGCCGATGTGCAGGTTGCGATCAATCGCGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCg                                                                                                                                                >  1:122004/1‑149 (MQ=255)
    cAGGCGGGGGCCACCCTGTCAGCCTACCTTGAGCAGCGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTaa                                                                                                                                             <  2:220750/149‑1 (MQ=255)
     aGGCGGGGGCCACCCTGTCAGCCTACCTTGAGCAGCGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAAc                                                                                                                                            >  4:201395/1‑149 (MQ=255)
            ggCCACCCTGTCAGCCTACCTTGAGCAGCGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGc                                                                                                                                     <  7:206293/149‑1 (MQ=255)
               cACCCTGTCAGCCTACCTTGAGCAGCGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAg                                                                                                                                  >  3:45063/1‑149 (MQ=255)
                       cAGCCTACCTTGAGCAGCGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTAcggcg                                                                                                                          >  6:201578/1‑149 (MQ=255)
                          ccTACCTTGAGCAGCGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTg                                                                                                                       <  5:291840/149‑1 (MQ=255)
                           cTACCTTGAGCAGCGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCaa                                                                                                                                   >  3:181754/1‑136 (MQ=255)
                           cTACCTTGAGCAGCGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCaa                                                                                                                                   <  4:181754/136‑1 (MQ=255)
                           cTACCTTGAGCAGCGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGc                                                                                                                      >  1:214513/1‑149 (MQ=255)
                           cTACCTTGAGCAGCGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGc                                                                                                                      <  4:184436/149‑1 (MQ=255)
                           cTACCTTGAGCAGCGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTAGAAGAGGAAGTGCGTAACGATCCGCAAGTTAcggcggggg                                                                                                                      >  1:281473/1‑146 (MQ=255)
                              ccTTGAGCAGCGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGt                                                                                                                        >  4:7481/1‑144 (MQ=255)
                              ccTTGAGCAGCGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGt                                                                                                                        <  3:7481/144‑1 (MQ=255)
                               cTTGAGCAGCGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGAt                                                                                                                  <  5:201578/149‑1 (MQ=255)
                               cTTGAGCAGCGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGAt                                                                                                                  >  3:72356/1‑149 (MQ=255)
                                ttGAGCAGCGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGATc                                                                                                                 >  2:53153/1‑149 (MQ=255)
                                    gcagcGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTg                                                                                                             >  2:171211/1‑149 (MQ=255)
                                      agcGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTgcc                                                                                                           <  4:72356/149‑1 (MQ=255)
                                               cTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGAGGTGCTGATCGCTGCCGCCAATAGt                                                                                                  >  5:245262/1‑149 (MQ=255)
                                                  ccgccgATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTGCCGCCAATAGTg                                                                                                 <  3:201395/147‑1 (MQ=255)
                                                    gccgATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTGCCGCCAATAGTGCgg                                                                                              >  3:238690/1‑148 (MQ=255)
                                                    gccgATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTGCCGCCAATAGTGCgg                                                                                              >  6:220512/1‑148 (MQ=255)
                                                      cgATGGTGGGCCGCTGATGGCCGATGAGCAGCTTGCGATCAATCGAGCGCTGAACACCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGATACGGCGGTGCTGATCGCTGCCGCCAATAGTGAGGCAt                                                                                           >  8:296170/1‑149 (MQ=255)
                                                            tggGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTGCCGCCAATAGTGCGGCATTGCAcc                                                                                     <  4:45063/149‑1 (MQ=255)
                                                                      ctgGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTGCCGCCAATAGTGCGGCATTGCACCGTGAAGccc                                                                            <  3:5801/148‑1 (MQ=255)
                                                                                 gCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTGCCGCCAATAGTGCGGCATTGCACCGTGAAGCCCCGGTGCAGACGt                                                                >  6:259948/1‑149 (MQ=255)
                                                                                     gTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTAcggc                                                                                                                           <  5:208174/86‑1 (MQ=255)
                                                                                     gTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTAGAAGAGGAAGTGCGTAACGATCCGCAAGTTAcggc                                                                                                                           >  6:208174/1‑86 (MQ=255)
                                                                                         cGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTGCCGCCAATAGTGCGGCATTGCACCGTGAAGCCCCGGTGCAGACGTTGCTGCAg                                                        <  1:171211/149‑1 (MQ=255)
                                                                                          gATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTGCCGCCAATAGTGCGGCATTGCACCGTGAAGCCCCGGTGCAGACGTTGCTGCAgg                                                       >  3:317149/1‑149 (MQ=255)
                                                                                            tCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTGCCGCCAATAGTGCGGCATTGCACCGTGAAGCCCCGGTGCAGACGTTGCTGCAGGcc                                                     >  3:197737/1‑149 (MQ=255)
                                                                                             cAATCGAGCGCTGAACCCCTAGTGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCATGTTACGGCGTTGCTGATCGCTGCCGCCAATAGTGCGGCATTGCACCGTGAAGCCCCGGTGCAGACGTTGCTGCAGGcc                                                     <  2:281473/148‑1 (MQ=255)
                                                                                                          aaCCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGAGGTGCTGATCGCTGCCGCCAATAGTGCGGCATTGCACCGTGAAGCCCCGGTGCAGACGTTGCTGCAGGCCCTGAACAAGCtt                                         <  6:245262/147‑1 (MQ=255)
                                                                                                            cccgcAGGGCCTGAACATCAGGGTGCTAGAAGAGGAAGTGCGTAACGATCCGAAAGTTATGGCGGTGCTGATCGCTGCCGCCAATAGTGCGGAATTGCACCGTGAAGCCCCGGTGCAGACGTTGCTGCAGGCCCTGAACAAGCTTGGCa                                     <  4:197737/149‑1 (MQ=255)
                                                                                                             ccccAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTGCCGCCAATAGTGCGGCATTGCACCGTGAAGCCCCGGTGCAGACGTTGCTGCAGGCCCTGAACAAGCTTGGCAg                                    >  8:135594/1‑149 (MQ=255)
                                                                                                                               aGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTGCCGCCAATAGTGCGGCATTGCACCGTGAAGCCCCGGTGCAGACGTTGCTGCAGGCCCTGAACAAGCTTGGCAGTACCCAAAGCATGAAcc                   <  5:220512/148‑1 (MQ=255)
                                                                                                                               aGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTGCCGCCAATAGTGCGGCATTGCACCGTGAAGCCCCGGTGCAGACGTTGCTGCAGGCCCTGAACAAGCTTGGCAGTACCCAAAGCATGAAc                    <  2:122004/147‑1 (MQ=255)
                                                                                                                                gggTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTGCCGCCAATAGTGCGGCATTGCACCGTGAAGCCCCGGTGCAGACGTTGCTGCAGGCCCTGAACAAGCTTGGCAGTACCCAAAGCATGAACCTg                 <  4:238690/149‑1 (MQ=255)
                                                                                                                                    gCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTGCCGCCAATAGTGCGGCATTGCACCGTGAAGCCCCGGTGCAGACGTTGCTGCAGGCCCTGAACAAGCTTGGCAGTACCCAAAGCATGAACCTGATcc             >  3:235678/1‑149 (MQ=255)
                                                                                                                                              gAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTGCCGCCAATAGTGCGGCATTGCACCGTGAAGCCCCGGTGCAGACGTTGCTGCAGGCCCTGAACAAGCTTGGCAGTACCCAAAGCATGAACCTGATCCTGGGCATGAc   >  5:109574/1‑149 (MQ=255)
                                                                                                                                              gAAGTACGTAACGATCCGCAATTTACGGCGGTGCTGATCGCTGCCGCCAATAGTGCGGAATTGCACCGTGAAGCCCCGGTGCAGACGTTGCTGCAGGCACTGAACAACCATGGCAGTAACCAAAGCAAGAACCTGATCCTGGGCATGAc   >  8:258291/1‑149 (MQ=255)
                                                                                                                                              gAAGTACGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTGCCGCCAATAGTGCGGCATTGCACCGTGAAGCCCCGGTGCAGACGTTGCTGCAGGCCCTGAACAAGCTTGGCAGTACCCAAAGCATGAACCTGATCCTGGGCATGAc   >  2:22867/1‑149 (MQ=255)
                                                                                                                                               aaGTACGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTGCCGCCAATAGTGCGGCATTGCACCGTGAAGCCCCGGTGCAGACGTTGCTGCAGGCCCTGAACAAGCTTGGCAGTACCCAAAGCATGAACCTGATCCTGGGCATGAcc  <  1:22867/149‑1 (MQ=255)
                                                                                                                                                aGTACGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTGCCGCCAATAGTGCGGCATTGCACCGTGAAGCCCCGGTGCAGACGTTGCTGCAGGCCCTGAACAAGCTTGGCAGTACCCAAAGCATGAACCTGATCCTGGGCATGAcc  <  7:258291/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GTTGCAGGCGGGGGCCACCCTGTCAGCCTACCTTGAGCAGCGTCGCGCTACCGCCGATGGTGGGCCGCTGCTGGCCGATGTGCAGGTTGCGATCAATCGAGCGCTGAACCCCCAGGGCCTGAACCTCAGGGTGCTCGAAGAGGAAGTGCGTAACGATCCGCAAGTTACGGCGGTGCTGATCGCTGCCGCCAATAGTGCGGCATTGCACCGTGAAGCCCCGGTGCAGACGTTGCTGCAGGCCCTGAACAAGCTTGGCAGTACCCAAAGCATGAACCTGATCCTGGGCATGACC  >  NC_002947/266704‑266995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑