BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,332,043	C→G	5.4%	A196G (GCC→GGC)	PP_2049 →	alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,332,043	0	C	G	5.4%	341.8 / 5.0	104	A196G (GCC→GGC)	PP_2049	alcohol dehydrogenase
Reads supporting (aligned to +/- strand): ref base C (50/48); new base G (4/2); total (54/50)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

GAGGTGACGCCGATCGCCATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGCCAGGC  >  NC_002947/2331895‑2332187

gAGGTGACGCCGATCGCCATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGc                                                                                                                                                  >  3:281697/1‑149 (MQ=255)

      aCGCCGATCGCCGTCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGt                                                                                                                                            <  6:247684/149‑1 (MQ=255)

      aCGCCGATCGCCATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGt                                                                                                                                            >  2:71575/1‑149 (MQ=255)

          cGATCGCCATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCAt                                                                                                                                        >  4:133343/1‑149 (MQ=255)

          cGATCGCCATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCAt                                                                                                                                        <  4:250932/149‑1 (MQ=255)

          cGATCGCCATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCAt                                                                                                                                        <  1:43792/149‑1 (MQ=255)

            aTCGCCATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGc                                                                                                                                      >  1:303609/1‑149 (MQ=255)

               gCCATCGTCACCACCGGCGCCACCACCAATTTGGGCATTTTTTCGCCGCTGCTGTTGCCATACCTGGCAGTGCTCGACGCCGTCT‑GCACCTATCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCa                                                                                                                                    <  1:255361/149‑1 (MQ=255)

               gCCATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCa                                                                                                                                    <  5:297810/148‑1 (MQ=255)

               gCCATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCAt                                                                                                                                   <  8:274997/149‑1 (MQ=255)

                ccATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCATTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATc                                                                                                                                  >  7:153433/1‑149 (MQ=255)

                ccATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCAt                                                                                                                                   >  5:266173/1‑148 (MQ=255)

                ccATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATc                                                                                                                                  >  3:123275/1‑149 (MQ=255)

                  aTCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCg                                                                                                                                 >  1:303309/1‑148 (MQ=255)

                     gTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGc                                                                                                                             <  4:281697/149‑1 (MQ=255)

                      tCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGcc                                                                                                                            <  8:216614/149‑1 (MQ=255)

                      tCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGcc                                                                                                                            >  6:243442/1‑149 (MQ=255)

                        accaccGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCta                                                                                                                          <  5:243442/149‑1 (MQ=255)

                          ccccGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCtata                                                                                                                        <  4:297287/147‑1 (MQ=255)

                          caccGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCtata                                                                                                                        >  3:297287/1‑149 (MQ=255)

                          caccGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCtata                                                                                                                        <  4:226398/149‑1 (MQ=255)

                            ccGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATAc                                                                                                                       >  8:11905/1‑148 (MQ=255)

                             cGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATAc                                                                                                                       >  6:270170/1‑147 (MQ=255)

                               gcgcCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAATGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAgc                                                                                                                   <  1:160726/149‑1 (MQ=255)

                               gcgcCACCACCAAGATCGGCACTGTTTCGCCGCCGCTGTCGCCAGACCTGGCAGTGCTCGACGCCGGCT‑GCACC‑CTCGGCCGGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAgc                                                                                                                   <  8:272512/149‑1 (MQ=255)

                                       accaAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTcc                                                                                                            >  2:111757/1‑147 (MQ=255)

                                         caAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAAc                                                                                                         >  5:188416/1‑149 (MQ=255)

                                              tGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAAc                                                                                                    <  8:204119/149‑1 (MQ=255)

                                                 gCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTAT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCg                                                                                                 >  3:34235/1‑149 (MQ=255)

                                                  cATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGc                                                                                                >  7:305692/1‑149 (MQ=255)

                                                   aTTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATgg                                                                                                                                             >  7:286886/1‑103 (MQ=255)

                                                   aTTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATgg                                                                                                                                             <  8:286886/103‑1 (MQ=255)

                                                   aTTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCt                                                                                               >  6:167224/1‑149 (MQ=255)

                                                      gTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTc                                                                                            >  5:68665/1‑149 (MQ=255)

                                                           gccgcTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGcc                                                                                       >  5:259884/1‑149 (MQ=255)

                                                              gctgctGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCctgc                                                                                    >  3:158785/1‑149 (MQ=255)

                                                                tgctgTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGt                                                                                                                                            >  8:169338/1‑91 (MQ=255)

                                                                tgctgTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGt                                                                                                                                            <  7:169338/91‑1 (MQ=255)

                                                                  ctgTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGc                                                                                >  8:166061/1‑149 (MQ=255)

                                                                    gTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGc                                                                                        <  5:99247/139‑1 (MQ=255)

                                                                    gTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGc                                                                                        >  6:99247/1‑139 (MQ=255)

                                                                       gCCAGACCTGGCAGTGCTCGACGCCGTCTGGCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGt                                                                            >  1:30272/1‑149 (MQ=255)

                                                                        ccAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGa                                                                          <  1:111757/149‑1 (MQ=255)

                                                                        ccAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGa                                                                          <  5:266854/149‑1 (MQ=255)

                                                                         cAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAg                                                                         <  7:241667/149‑1 (MQ=255)

                                                                                ggCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGc                                                                  <  3:133343/149‑1 (MQ=255)

                                                                                 gCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTg                                                                   <  5:103352/147‑1 (MQ=255)

                                                                                 gCAGTGCTCCACGCCCTCT‑GCACC‑CTCGGCCTCCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGCTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCGGCTGGCCCGTGAGGCCCTGcc                                                                 >  7:221352/1‑148 (MQ=255)

                                                                                   aGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGgc                                                               >  7:4606/1‑149 (MQ=255)

                                                                                   aGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCAgc                                                               <  2:303609/149‑1 (MQ=255)

                                                                                        tCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGa                                                                          >  8:249313/1‑133 (MQ=255)

                                                                                        tCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGctgctg                                                          >  8:177514/1‑149 (MQ=255)

                                                                                        tCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGctgctg                                                          >  5:244937/1‑149 (MQ=255)

                                                                                        tCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGctgctg                                                          >  7:271279/1‑149 (MQ=255)

                                                                                         ggACGCGTTCT‑GCTCG‑GTCGGCCTGCTATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATTGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGctgctg                                                          <  6:244937/147‑1 (MQ=255)

                                                                                          gACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGc                                                                                                                             >  5:307610/1‑80 (MQ=255)

                                                                                          gACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGc                                                                                                                             <  6:307610/80‑1 (MQ=255)

                                                                                          gACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGc                                                        >  4:100623/1‑149 (MQ=255)

                                                                                          gACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGc                                                        <  7:166061/149‑1 (MQ=255)

                                                                                            cGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCgg                                                      <  4:158785/149‑1 (MQ=255)

                                                                                               cGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAgccgcc                                                                                                               >  8:162569/1‑89 (MQ=255)

                                                                                               cGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAgccgcc                                                                                                               <  7:162569/89‑1 (MQ=255)

                                                                                                      cACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCtt                                              <  7:177514/148‑1 (MQ=255)

                                                                                                       aCC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTg                                                                   >  3:35897/1‑126 (MQ=255)

                                                                                                       aCC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGa                                            >  6:270073/1‑149 (MQ=255)

                                                                                                       aCC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGa                                            <  2:303309/149‑1 (MQ=255)

                                                                                                       aCC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGa                                            <  4:34235/149‑1 (MQ=255)

                                                                                                       aCC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCAGGGTGCCTGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGGGAGCCGCTGCGGCTGCTGGCGGATAACCTGGa                                            >  5:192699/1‑149 (MQ=255)

                                                                                                       aCC‑CTCGGCCGGCCATCGGCGGTGAGTGCTGCTACCGGTAGCGATGCCATGGTGCATGCCATCGAAGCCTTTACCAGCCGCCTCATTCGTAACGCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTgt                                            <  8:221352/149‑2 (MQ=255)

                                                                                                         c‑cTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGAcc                                          >  3:3170/1‑149 (MQ=255)

                                                                                                         c‑cTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGAc                                           >  7:258264/1‑148 (MQ=255)

                                                                                                           cTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGAc                                           >  1:238784/1‑147 (MQ=255)

                                                                                                               gCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCt                                     <  5:270170/149‑1 (MQ=255)

                                                                                                                    ccATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCtgtg                                  <  5:270073/147‑1 (MQ=255)

                                                                                                                    ccATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGca                                >  5:192929/1‑149 (MQ=255)

                                                                                                                    ccATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGca                                >  6:88174/1‑149 (MQ=255)

                                                                                                                     cATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGc                                                        >  2:230521/1‑124 (MQ=255)

                                                                                                                     cATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGca                                >  5:163695/1‑148 (MQ=255)

                                                                                                                       tCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACaa                             <  7:66026/149‑1 (MQ=255)

                                                                                                                          gcggTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAAcgg                          <  5:167224/149‑1 (MQ=255)

                                                                                                                             gTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCg                                                       <  3:10737/117‑1 (MQ=255)

                                                                                                                             gTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCg                                                       >  4:10737/1‑117 (MQ=255)

                                                                                                                                aCTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCaa                    >  8:23058/1‑149 (MQ=255)

                                                                                                                                     tgctACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAAcggcgg                       >  8:24213/1‑141 (MQ=255)

                                                                                                                                     tgctACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAAcggcgg                       <  7:24213/141‑1 (MQ=255)

                                                                                                                                       ctACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGc               <  3:100623/147‑1 (MQ=255)

                                                                                                                                           cGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGccc         >  2:325532/1‑149 (MQ=255)

                                                                                                                                            ggTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCtgtg                                  >  1:44333/1‑123 (MQ=255)

                                                                                                                                            ggTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCtgtg                                  <  2:44333/123‑1 (MQ=255)

                                                                                                                                             gcttCGATGGCATGCACCATGGCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCtgtg                                  <  5:80692/119‑1 (MQ=255)

                                                                                                                                             gcttCGATGGCATGCACCATGGCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCtgtg                                  >  6:80692/4‑122 (MQ=255)

                                                                                                                                             gTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGc       >  7:194148/1‑149 (MQ=255)

                                                                                                                                              tATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGcc      <  1:325532/149‑1 (MQ=255)

                                                                                                                                              cttCGATGGCATGCACCATGGCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGc       >  1:218375/3‑148 (MQ=255)

                                                                                                                                               ttCGATGGCATGCACCATGGCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCt                                                                                               <  2:6464/58‑1 (MQ=21)

                                                                                                                                               ttCGATGGCATGCACCATGGCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCt                                                                                               >  1:6464/2‑59 (MQ=21)

                                                                                                                                               ttCGATGGCATGCACCATGGCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGc       >  5:50299/2‑147 (MQ=255)

                                                                                                                                                tCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGCCAg    <  5:88174/149‑1 (MQ=255)

                                                                                                                                                  gATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGCCAGGc  <  4:3170/149‑1 (MQ=255)

                                                                                                                                                   aTGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGCCAGGc  >  6:186391/1‑148 (MQ=255)

GAGGTGACGCCGATCGCCATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGCCAGGC  >  NC_002947/2331895‑2332187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`