BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,332,049	T→A	5.9%	V198E (GTG→GAG) ‡	PP_2049 →	alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,332,049	0	T	A	5.9%	298.9 / 6.3	101	V198E (GTG→GAG) ‡	PP_2049	alcohol dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (50/45); new base A (4/2); total (54/47)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

Only 100 of 107 total aligned reads displayed.

ACGCCGATCGCCATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGCCAGGCCATGCTGTTG  >  NC_002947/2331901‑2332197

aCGCCGATCGCCGTCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGt                                                                                                                                                      <  6:247684/149‑1 (MQ=255)

aCGCCGATCGCCATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGt                                                                                                                                                      >  2:71575/1‑149 (MQ=255)

aCGCCGATCGCCATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGt                                                                                                                                                      <  6:154305/149‑1 (MQ=255)

    cGATCGCCATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCAt                                                                                                                                                  >  4:133343/1‑149 (MQ=255)

    cGATCGCCATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCAt                                                                                                                                                  <  4:250932/149‑1 (MQ=255)

    cGATCGCCATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCAt                                                                                                                                                  <  1:43792/149‑1 (MQ=255)

      aTCGCCATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGc                                                                                                                                                >  1:303609/1‑149 (MQ=255)

         gCCATCGTCACCACCGGCGCCACCACCAATTTGGGCATTTTTTCGCCGCTGCTGTTGCCATACCTGGCAGTGCTCGACGCCGTCT‑GCACCTATCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCa                                                                                                                                              <  1:255361/149‑1 (MQ=255)

         gCCATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCa                                                                                                                                              <  5:297810/148‑1 (MQ=255)

         gCCATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCAt                                                                                                                                             <  8:274997/149‑1 (MQ=255)

          ccATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCAt                                                                                                                                             >  5:266173/1‑148 (MQ=255)

          ccATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATc                                                                                                                                            >  3:123275/1‑149 (MQ=255)

            aTCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCg                                                                                                                                           >  1:303309/1‑148 (MQ=255)

               gTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGc                                                                                                                                       <  4:281697/149‑1 (MQ=255)

                tCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGcc                                                                                                                                      >  6:243442/1‑149 (MQ=255)

                tCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGcc                                                                                                                                      <  8:216614/149‑1 (MQ=255)

                  accaccGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCta                                                                                                                                    <  5:243442/149‑1 (MQ=255)

                    ccccGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCtata                                                                                                                                  <  4:297287/147‑1 (MQ=255)

                    caccGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCtata                                                                                                                                  <  4:226398/149‑1 (MQ=255)

                    caccGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCtata                                                                                                                                  >  3:297287/1‑149 (MQ=255)

                       cGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATAc                                                                                                                                 >  6:270170/1‑147 (MQ=255)

                         gcgcCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAATGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAgc                                                                                                                             <  1:160726/149‑1 (MQ=255)

                         gcgcCACCACCAAGATCGGCACTGTTTCGCCGCCGCTGTCGCCAGACCTGGCAGTGCTCGACGCCGGCT‑GCACC‑CTCGGCCGGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAgc                                                                                                                             <  8:272512/149‑1 (MQ=255)

                                 accaAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTcc                                                                                                                      >  2:111757/1‑147 (MQ=255)

                                        tGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAAc                                                                                                              <  8:204119/149‑1 (MQ=255)

                                           gCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTAT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCg                                                                                                           >  3:34235/1‑149 (MQ=255)

                                            cATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGc                                                                                                          >  7:305692/1‑149 (MQ=255)

                                            cATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGc                                                                                                          <  2:76411/149‑1 (MQ=255)

                                             aTTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCt                                                                                                         >  6:167224/1‑149 (MQ=255)

                                                gTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTc                                                                                                      >  5:68665/1‑149 (MQ=255)

                                                     gccgcTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGcc                                                                                                 >  5:259884/1‑149 (MQ=255)

                                                        gctgctGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCctgc                                                                                              >  3:158785/1‑149 (MQ=255)

                                                          tgctgTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGt                                                                                                                                                      >  8:169338/1‑91 (MQ=255)

                                                          tgctgTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGt                                                                                                                                                      <  7:169338/91‑1 (MQ=255)

                                                            ctgTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGc                                                                                          >  8:166061/1‑149 (MQ=255)

                                                              gTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGc                                                                                                  >  6:99247/1‑139 (MQ=255)

                                                                 gCCAGACCTGGCAGTGCTCGACGCCGTCTGGCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGt                                                                                      >  1:30272/1‑149 (MQ=255)

                                                                  ccAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGa                                                                                    <  1:111757/149‑1 (MQ=255)

                                                                  ccAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGa                                                                                    <  5:266854/149‑1 (MQ=255)

                                                                   cAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAg                                                                                   <  7:241667/149‑1 (MQ=255)

                                                                          ggCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGc                                                                            <  3:133343/149‑1 (MQ=255)

                                                                           gCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTg                                                                             <  5:103352/147‑1 (MQ=255)

                                                                           gCAGTGCTCCACGCCCTCT‑GCACC‑CTCGGCCTCCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGCTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCGGCTGGCCCGTGAGGCCCTGcc                                                                           >  7:221352/1‑148 (MQ=255)

                                                                             aGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGgc                                                                         >  7:4606/1‑149 (MQ=255)

                                                                             aGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCAgc                                                                         <  2:303609/149‑1 (MQ=255)

                                                                                  tCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGa                                                                                    >  8:249313/1‑133 (MQ=255)

                                                                                  tCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGa                                                                                    <  7:249313/133‑1 (MQ=255)

                                                                                  tCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGctgctg                                                                    >  5:244937/1‑149 (MQ=255)

                                                                                  tCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGctgctg                                                                    >  7:271279/1‑149 (MQ=255)

                                                                                  tCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGctgctg                                                                    >  8:177514/1‑149 (MQ=255)

                                                                                   ggACGCGTTCT‑GCTCG‑GTCGGCCTGCTATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATTGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGctgctg                                                                    <  6:244937/147‑1 (MQ=255)

                                                                                    gACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGc                                                                                                                                       <  6:307610/80‑1 (MQ=255)

                                                                                    gACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGc                                                                                                                                       >  5:307610/1‑80 (MQ=255)

                                                                                    gACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGc                                                                  >  4:100623/1‑149 (MQ=255)

                                                                                    gACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGc                                                                  <  7:166061/149‑1 (MQ=255)

                                                                                      cGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCgg                                                                <  4:158785/149‑1 (MQ=255)

                                                                                         cGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAgccgcc                                                                                                                         <  7:162569/89‑1 (MQ=255)

                                                                                         cGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAgccgcc                                                                                                                         >  8:162569/1‑89 (MQ=255)

                                                                                                cACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCtt                                                        <  7:177514/148‑1 (MQ=255)

                                                                                                 aCC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTg                                                                             <  4:35897/126‑1 (MQ=255)

                                                                                                 aCC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGa                                                      <  4:34235/149‑1 (MQ=255)

                                                                                                 aCC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGa                                                      >  6:270073/1‑149 (MQ=255)

                                                                                                 aCC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCAGGGTGCCTGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGGGAGCCGCTGCGGCTGCTGGCGGATAACCTGGa                                                      >  5:192699/1‑149 (MQ=255)

                                                                                                 aCC‑CTCGGCCGGCCATCGGCGGTGAGTGCTGCTACCGGTAGCGATGCCATGGTGCATGCCATCGAAGCCTTTACCAGCCGCCTCATTCGTAACGCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTgt                                                      <  8:221352/149‑2 (MQ=255)

                                                                                                   c‑cTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGAcc                                                    >  3:3170/1‑149 (MQ=255)

                                                                                                   c‑cTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGAc                                                     >  7:258264/1‑148 (MQ=255)

                                                                                                     cTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGAc                                                     >  1:238784/1‑147 (MQ=255)

                                                                                                         gCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCt                                               <  5:270170/149‑1 (MQ=255)

                                                                                                              ccATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCtgtg                                            <  5:270073/147‑1 (MQ=255)

                                                                                                              ccATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGca                                          >  6:88174/1‑149 (MQ=255)

                                                                                                              ccATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGca                                          >  5:192929/1‑149 (MQ=255)

                                                                                                               cATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGc                                                                  >  2:230521/1‑124 (MQ=255)

                                                                                                               cATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGca                                          >  5:163695/1‑148 (MQ=255)

                                                                                                                 tCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACaa                                       <  7:66026/149‑1 (MQ=255)

                                                                                                                    gcggTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAAcgg                                    <  5:167224/149‑1 (MQ=255)

                                                                                                                       gTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCg                                                                 >  4:10737/1‑117 (MQ=255)

                                                                                                                       gTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCg                                                                 <  3:10737/117‑1 (MQ=255)

                                                                                                                          aCTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCaa                              >  8:23058/1‑149 (MQ=255)

                                                                                                                               tgctACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAAcggcgg                                 >  8:24213/1‑141 (MQ=255)

                                                                                                                               tgctACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAAcggcgg                                 <  7:24213/141‑1 (MQ=255)

                                                                                                                                 ctACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGc                         <  3:100623/147‑1 (MQ=255)

                                                                                                                                     cGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGccc                   >  2:325532/1‑149 (MQ=255)

                                                                                                                                      ggTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCtgtg                                            <  2:44333/123‑1 (MQ=255)

                                                                                                                                      ggTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCtgtg                                            >  1:44333/1‑123 (MQ=255)

                                                                                                                                       gcttCGATGGCATGCACCATGGCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCtgtg                                            >  6:80692/4‑122 (MQ=255)

                                                                                                                                       gcttCGATGGCATGCACCATGGCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCtgtg                                            <  5:80692/119‑1 (MQ=255)

                                                                                                                                       gTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGc                 >  7:194148/1‑149 (MQ=255)

                                                                                                                                        tATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGcc                <  1:325532/149‑1 (MQ=255)

                                                                                                                                        cttCGATGGCATGCACCATGGCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGc                 >  1:218375/3‑148 (MQ=255)

                                                                                                                                         ttCGATGGCATGCACCATGGCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCt                                                                                                         >  1:6464/2‑59 (MQ=21)

                                                                                                                                         ttCGATGGCATGCACCATGGCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCt                                                                                                         <  2:6464/58‑1 (MQ=21)

                                                                                                                                         ttCGATGGCATGCACCATGGCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGc                 >  5:50299/2‑147 (MQ=255)

                                                                                                                                          tCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGCCAg              <  5:88174/149‑1 (MQ=255)

                                                                                                                                            gATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGCCAGGc            <  4:3170/149‑1 (MQ=255)

                                                                                                                                             aTGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGCCAGGc            >  6:186391/1‑148 (MQ=255)

                                                                                                                                                 cATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGCCAGGCCATGc       >  8:149189/1‑149 (MQ=255)

                                                                                                                                                  aTGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGCCAGGCCATGCt      >  6:149702/1‑149 (MQ=255)

                                                                                                                                                  aTGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGCCAGGCCATGCt      >  1:192189/1‑149 (MQ=255)

                                                                                                                                                     gTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGCCAGGCCATGCTGtt   <  8:258264/149‑1 (MQ=255)

                                                                                                                                                      tGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGCCAGGCCATGCTGTTg  <  6:259884/149‑1 (MQ=255)

ACGCCGATCGCCATCGTCACCACCGGCGCCACCACCAAGATGGGCATTGTTTCGCCGCTGCTGTTGCCAGACCTGGCAGTGCTCGACGCCGTCT‑GCACC‑CTCGGCCTGCCATCGGCGGTGACTGCTGCTACCGGTATCGATGCCATGGTGCATGCCATCGAAGCCTATACCAGCCGCCTCAAACGTAACCCGCTGTCCAGCCTGCTGGCCCGTGAGGCGCTGCGGCTGCTGGCGGATAACCTTGACCAGGCTGTGCACAACGGCGGCAACCTGCAGGCCCGCCAGGCCATGCTGTTG  >  NC_002947/2331901‑2332197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`