Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,360,180	T→C	5.3%	K326R (AAG→AGG)	PP_2075 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,360,180	0	T	C	5.3%	217.0 / 2.2	75	K326R (AAG→AGG)	PP_2075	membrane protein
Reads supporting (aligned to +/- strand):  ref base T (32/39);  new base C (2/2);  total (34/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.91e-01

TCGTTGGCCTGTTGAGGAGTAGAGCGCTTGCGTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAACAGCCAGCTGCTTTTGCGGAA  >  NC_002947/2360033‑2360328                                                                                                                                                    |                                                                                                                                                     tCGTTGGCCTGTTGAGGAGTAGAGCGCTTGCGTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCt                                                                                                                                                      <  4:151750/148‑1 (MQ=255)       gCCTGTTGAGGAGTAGAGCGCTTGCGTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCggg                                                                                                                                               >  6:134286/1‑149 (MQ=255)       gCCTGTTGAGGAGTAGAGCGCTTGCGTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCggg                                                                                                                                               <  7:93510/149‑1 (MQ=255)          tGTTGAGGAGTAGAGCGCTTGCGTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAg                                                                                                                                            >  2:269025/1‑149 (MQ=255)                ggagTAGAGCGCTTGCGTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAggccggc                                                                                                                                      <  6:1806/149‑1 (MQ=255)                 gagTAGAGCGCTTGCGTGGTGTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAggccggcc                                                                                                                                     <  6:235026/149‑1 (MQ=255)                       agCGCTTGCGTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCt                                                                                                                                                      >  1:330684/1‑126 (MQ=40)                       agCGCTTGCGTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCt                                                                                                                                                      <  2:330684/126‑1 (MQ=40)                        gcgcTTGCGTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGa                                                                                                                              <  6:163847/149‑1 (MQ=38)                          gcTTGCGTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATg                                                                                                                            <  7:93286/149‑1 (MQ=35)                                gTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTc                                                                                                                      >  3:10438/1‑149 (MQ=34)                                gTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTc                                                                                                                      <  2:240563/149‑1 (MQ=34)                                 tGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCg                                                                                                                     >  2:320884/1‑149 (MQ=34)                                      ttttCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGc                                                                                                                   >  4:236684/1‑146 (MQ=32)                                      ttttCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCa                                                                                                                 <  3:236684/148‑1 (MQ=32)                                      ttttCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGGGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCa                                                                                                                 <  2:80195/148‑1 (MQ=17)                                        ttCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAgc                                                                                                               <  4:173747/148‑1 (MQ=32)                                          cGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAgcgc                                                                                                             <  7:179853/148‑1 (MQ=32)                                           gccggccgCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTGGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCgg                                                                                                           <  7:43655/149‑1 (MQ=17)                                           gccggccgCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGcgcg                                                                                                            <  4:6589/148‑1 (MQ=32)                                            ccggccgCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGt                                                                                                          >  8:252196/1‑149 (MQ=32)                                               gccgCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGc                                                                                                       >  5:217611/1‑149 (MQ=32)                                                   cTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCcagc                                                                                                   >  5:245383/1‑149 (MQ=32)                                                   cTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCCTGCCCGGCAagcca                                                                                                                                        <  6:303979/112‑2 (MQ=255)                                                   cTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCCTGCCCGGCAagcca                                                                                                                                        >  5:303979/1‑111 (MQ=255)                                                       ccGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCcagcacag                                                                                               >  4:92987/1‑149 (MQ=32)                                                          ggCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCAcagcag                                                                                            >  7:28677/1‑149 (MQ=32)                                                           gCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCAcagcagc                                                                                           <  7:252196/149‑1 (MQ=32)                                                            cTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCAcagcag                                                                                            >  2:51557/1‑147 (MQ=32)                                                                aGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCt                                                                                      <  7:88606/149‑1 (MQ=32)                                                                 gCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTg                                                                                     <  3:92987/149‑1 (MQ=32)                                                                     ccAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGc                                                                                  >  1:179999/1‑148 (MQ=32)                                                                       aGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCCTGCCCGGCAagccagt                                                                                                                                      >  8:203402/1‑91 (MQ=11)                                                                       aGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCCTGCCCGGCAagccagt                                                                                                                                      <  7:203402/94‑4 (MQ=11)                                                                         cGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTg                                                                             >  1:53899/1‑149 (MQ=32)                                                                           gTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGCTTGTCACGGGTGGGACTGGCTAGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGACAGCACAGCAGCAGGCTGAGCCAGTggg                                                                           >  1:192717/1‑149 (MQ=12)                                                                            tCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGc                                                                          >  3:138204/1‑149 (MQ=33)                                                                              ggCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGa                                                                                    >  6:128683/1‑137 (MQ=32)                                                                              ggCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGa                                                                                    <  5:128683/137‑1 (MQ=32)                                                                               gCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGt                                                                       >  5:218444/1‑149 (MQ=34)                                                                                     gCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCgat                                                                 <  2:179999/149‑1 (MQ=34)                                                                                     gCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCgat                                                                 <  1:31541/149‑1 (MQ=34)                                                                                      cGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCgatg                                                                <  5:143055/149‑1 (MQ=34)                                                                                       gAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTTCCgatga                                                               <  8:21464/149‑1 (MQ=21)                                                                                       gAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCgatga                                                               >  2:201878/1‑149 (MQ=34)                                                                                       gAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCgatga                                                               >  5:29703/1‑149 (MQ=34)                                                                                        aGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCgatgat                                                              <  5:134286/149‑1 (MQ=34)                                                                                         gCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCgatgat                                                              >  4:250943/1‑148 (MQ=34)                                                                                          cAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGt                                                                       >  2:218117/1‑138 (MQ=34)                                                                                          cAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGt                                                                       <  1:218117/138‑1 (MQ=34)                                                                                          cAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGAtgatga                                                            <  6:217611/149‑1 (MQ=34)                                                                                          cAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGAtgatga                                                            <  1:51557/149‑1 (MQ=34)                                                                                           aGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGAc                                                           >  2:283427/1‑149 (MQ=34)                                                                                            ggCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGACCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGAc                                                           >  6:129672/1‑148 (MQ=21)                                                                                                 gCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGTGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAAcgc                                                     >  5:228381/1‑149 (MQ=37)                                                                                                   cTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCgg                                                   <  3:73738/149‑1 (MQ=37)                                                                                                          gCGGGCGCGTAGTCGCTCAGGCTTCTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGa                                             <  2:16255/148‑1 (MQ=255)                                                                                                           cGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGAc                                            <  1:201878/148‑1 (MQ=37)                                                                                                             ggggggtCGTCGCGCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGTGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACaaa                                         <  6:228381/146‑1 (MQ=255)                                                                                                              gtgggtAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAAc                                        <  6:218444/149‑1 (MQ=35)                                                                                                               tgggtAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAAca                                       >  7:213455/1‑149 (MQ=35)                                                                                                                  gtAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAAcagca                                    <  6:29703/149‑1 (MQ=34)                                                                                                                  gtAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAAcag                                      >  2:141954/1‑147 (MQ=34)                                                                                                                  gtAGGCGCTCAGGGTTGTCACGGGGGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAAcagca                                    <  4:291885/149‑1 (MQ=21)                                                                                                                   tAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGACTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAAcagcag                                   >  1:123048/1‑149 (MQ=21)                                                                                                                     gTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTg                                 <  2:53899/149‑1 (MQ=35)                                                                                                                      tCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTg                                 >  4:161903/1‑148 (MQ=35)                                                                                                                          tCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCgg                                                   >  6:247000/1‑126 (MQ=35)                                                                                                                          tCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCgg                                                   <  5:247000/126‑1 (MQ=35)                                                                                                                               gTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGaa                       <  1:320884/149‑1 (MQ=39)                                                                                                                               gTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGaa                       <  8:28677/149‑1 (MQ=39)                                                                                                                                ttGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGaa                       >  2:312814/1‑148 (MQ=39)                                                                                                                                  gTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAAca                     <  4:10438/148‑1 (MQ=39)                                                                                                                                  gTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAAca                     <  4:138204/148‑1 (MQ=39)                                                                                                                                   tCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAAcagc                   >  8:6143/1‑149 (MQ=39)                                                                                                                                     aCGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAAcagcc                  <  6:245383/148‑1 (MQ=39)                                                                                                                                        ggTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCCGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAACAGACAgct              >  5:94890/1‑149 (MQ=255)                                                                                                                                             gACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAACAGCCAGCTGCttt         <  4:50639/149‑1 (MQ=40)                                                                                                                                                    ttGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAACAGCCAGCTGCTTTTGCgg    >  5:182098/1‑147 (MQ=255)                                                                                                                                                    ttGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAACAGCCAGCTGCTTTTGCGgaa  >  6:83779/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     TCGTTGGCCTGTTGAGGAGTAGAGCGCTTGCGTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAACAGCCAGCTGCTTTTGCGGAA  >  NC_002947/2360033‑2360328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑