Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,360,185	G→C	5.1%	P324P (CCC→CCG)	PP_2075 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,360,185	0	G	C	5.1%	246.3 / 3.6	79	P324P (CCC→CCG)	PP_2075	membrane protein
Reads supporting (aligned to +/- strand):  ref base G (36/39);  new base C (2/2);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.74e-01

GCCTGTTGAGGAGTAGAGCGCTTGCGTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAACAGCCAGCTGCTTTTGCGGAAG  >  NC_002947/2360039‑2360329                                                                                                                                                   |                                                                                                                                                 gCCTGTTGAGGAGTAGAGCGCTTGCGTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCggg                                                                                                                                                >  6:134286/1‑149 (MQ=255) gCCTGTTGAGGAGTAGAGCGCTTGCGTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCggg                                                                                                                                                <  7:93510/149‑1 (MQ=255)    tGTTGAGGAGTAGAGCGCTTGCGTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAg                                                                                                                                             >  2:269025/1‑149 (MQ=255)          ggagTAGAGCGCTTGCGTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAggccggc                                                                                                                                       <  6:1806/149‑1 (MQ=255)           gagTAGAGCGCTTGCGTGGTGTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAggccggcc                                                                                                                                      <  6:235026/149‑1 (MQ=255)                  gcgcTTGCGTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGa                                                                                                                               <  6:163847/149‑1 (MQ=38)                    gcTTGCGTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATg                                                                                                                             <  7:93286/149‑1 (MQ=35)                          gTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTc                                                                                                                       >  3:10438/1‑149 (MQ=34)                          gTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTc                                                                                                                       <  2:240563/149‑1 (MQ=34)                           tGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCg                                                                                                                      >  2:320884/1‑149 (MQ=34)                                ttttCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGc                                                                                                                    >  4:236684/1‑146 (MQ=32)                                ttttCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCa                                                                                                                  <  3:236684/148‑1 (MQ=32)                                ttttCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGGGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCa                                                                                                                  <  2:80195/148‑1 (MQ=17)                                  ttCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAgc                                                                                                                <  4:173747/148‑1 (MQ=32)                                    cGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAgcgc                                                                                                              <  7:179853/148‑1 (MQ=32)                                     gccggccgCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTGGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCgg                                                                                                            <  7:43655/149‑1 (MQ=17)                                     gccggccgCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGcgcg                                                                                                             <  4:6589/148‑1 (MQ=32)                                      ccggccgCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGt                                                                                                           >  8:252196/1‑149 (MQ=32)                                         gccgCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGc                                                                                                        >  5:217611/1‑149 (MQ=32)                                             cTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCcagc                                                                                                    >  5:245383/1‑149 (MQ=32)                                             cTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCCTGCCCGGCAagcca                                                                                                                                         <  6:303979/112‑2 (MQ=255)                                             cTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCCTGCCCGGCAagcca                                                                                                                                         >  5:303979/1‑111 (MQ=255)                                                 ccGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCcagcacag                                                                                                >  4:92987/1‑149 (MQ=32)                                                    ggCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCAcagcag                                                                                             >  7:28677/1‑149 (MQ=32)                                                     gCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCAcagcagc                                                                                            <  7:252196/149‑1 (MQ=32)                                                      cTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCAcagcag                                                                                             >  2:51557/1‑147 (MQ=32)                                                          aGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCt                                                                                       <  7:88606/149‑1 (MQ=32)                                                           gCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTg                                                                                      <  3:92987/149‑1 (MQ=32)                                                               ccAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGc                                                                                   >  1:179999/1‑148 (MQ=32)                                                                 aGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCCTGCCCGGCAagccagt                                                                                                                                       <  7:203402/94‑4 (MQ=11)                                                                 aGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCCTGCCCGGCAagccagt                                                                                                                                       >  8:203402/1‑91 (MQ=11)                                                                   cGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTg                                                                              >  1:53899/1‑149 (MQ=32)                                                                     gTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGCTTGTCACGGGTGGGACTGGCTAGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGACAGCACAGCAGCAGGCTGAGCCAGTggg                                                                            >  1:192717/1‑149 (MQ=12)                                                                      tCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGc                                                                           >  3:138204/1‑149 (MQ=33)                                                                        ggCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGa                                                                                     >  6:128683/1‑137 (MQ=32)                                                                        ggCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGa                                                                                     <  5:128683/137‑1 (MQ=32)                                                                         gCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGt                                                                        >  5:218444/1‑149 (MQ=34)                                                                               gCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCgat                                                                  <  1:31541/149‑1 (MQ=34)                                                                               gCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCgat                                                                  <  2:179999/149‑1 (MQ=34)                                                                                cGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCgatg                                                                 <  5:143055/149‑1 (MQ=34)                                                                                 gAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTTCCgatga                                                                <  8:21464/149‑1 (MQ=21)                                                                                 gAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCgatga                                                                >  2:201878/1‑149 (MQ=34)                                                                                 gAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCgatga                                                                >  5:29703/1‑149 (MQ=34)                                                                                  aGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCgatgat                                                               <  5:134286/149‑1 (MQ=34)                                                                                   gCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCgatgat                                                               >  4:250943/1‑148 (MQ=34)                                                                                    cAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGt                                                                        >  2:218117/1‑138 (MQ=34)                                                                                    cAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGt                                                                        <  1:218117/138‑1 (MQ=34)                                                                                    cAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGAtgatga                                                             <  6:217611/149‑1 (MQ=34)                                                                                    cAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGAtgatga                                                             <  1:51557/149‑1 (MQ=34)                                                                                     aGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGAc                                                            >  2:283427/1‑149 (MQ=34)                                                                                      ggCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGACCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGAc                                                            >  6:129672/1‑148 (MQ=21)                                                                                           gCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGTGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAAcgc                                                      >  5:228381/1‑149 (MQ=37)                                                                                             cTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCgg                                                    <  3:73738/149‑1 (MQ=37)                                                                                                    gCGGGCGCGTAGTCGCTCAGGCTTCTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGa                                              <  2:16255/148‑1 (MQ=255)                                                                                                     cGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGAc                                             <  1:201878/148‑1 (MQ=37)                                                                                                       ggggggtCGTCGCGCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGTGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACaaa                                          <  6:228381/146‑1 (MQ=255)                                                                                                        gtgggtAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAAc                                         <  6:218444/149‑1 (MQ=35)                                                                                                         tgggtAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAAca                                        >  7:213455/1‑149 (MQ=35)                                                                                                            gtAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAAcagca                                     <  6:29703/149‑1 (MQ=34)                                                                                                            gtAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAAcag                                       >  2:141954/1‑147 (MQ=34)                                                                                                            gtAGGCGCTCAGGGTTGTCACGGGGGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAAcagca                                     <  4:291885/149‑1 (MQ=21)                                                                                                             tAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGACTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAAcagcag                                    >  1:123048/1‑149 (MQ=21)                                                                                                               gTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTg                                  <  2:53899/149‑1 (MQ=35)                                                                                                                tCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTg                                  >  4:161903/1‑148 (MQ=35)                                                                                                                    tCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCgg                                                    >  6:247000/1‑126 (MQ=35)                                                                                                                    tCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCgg                                                    <  5:247000/126‑1 (MQ=35)                                                                                                                         gTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGaa                        <  1:320884/149‑1 (MQ=39)                                                                                                                         gTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGaa                        <  8:28677/149‑1 (MQ=39)                                                                                                                          ttGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGaa                        >  2:312814/1‑148 (MQ=39)                                                                                                                            gTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAAca                      <  4:10438/148‑1 (MQ=39)                                                                                                                            gTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAAca                      <  4:138204/148‑1 (MQ=39)                                                                                                                             tCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAAcagc                    >  8:6143/1‑149 (MQ=39)                                                                                                                               aCGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAAcagcc                   <  6:245383/148‑1 (MQ=39)                                                                                                                                  ggTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCCGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAACAGACAgct               >  5:94890/1‑149 (MQ=255)                                                                                                                                       gACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAACAGCCAGCTGCttt          <  4:50639/149‑1 (MQ=40)                                                                                                                                           gcgaggCCGGCCTGCCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAACAGCCAGCTGCTTTTGCg      >  4:169522/6‑149 (MQ=255)                                                                                                                                              ttGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAACAGCCAGCTGCTTTTGCgg     >  5:182098/1‑147 (MQ=255)                                                                                                                                              ttGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAACAGCCAGCTGCTTTTGCGgaa   >  6:83779/1‑149 (MQ=255)                                                                                                                                                gCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACtt                            >  3:58790/1‑122 (MQ=40)                                                                                                                                                gCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACtt                            <  4:58790/122‑1 (MQ=40)                                                                                                                                                gCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAACAGCCAGCTGCTTTTGCGgaag  >  3:95960/1‑148 (MQ=255)                                                                                                                                                   gggCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACttt                           <  6:328903/120‑1 (MQ=255)                                                                                                                                                   gggCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACttt                           >  5:328903/1‑120 (MQ=255)                                                                                                                                                   |                                                                                                                                                 GCCTGTTGAGGAGTAGAGCGCTTGCGTGGTTTTTTCGCCGGCCGCTTGCCGGGCTTGAGCTGCCAGCGGTCGGCGTTGGCGAGCAGGCGTGCCTGGATTGCGGGTGGGTAGTCGCTCAGGGTTGTCACGGGTGGGACTGGCTTGCCGGGCAGGCCGGCCTCGCGGATGACCTTCGGCCAGCGCGGTCGCCAGCACAGCAGCAGGCTGAGCCAGTGGGCGGTGCCGATGATGACGAACGCGGGCCAGACAAACAGCAGTGCAACTTTGAACAGCCAGCTGCTTTTGCGGAAG  >  NC_002947/2360039‑2360329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑