Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,363,386 T→A 7.6% D164V (GAC→GTC)  PP_2076 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,363,3860TA 7.6% 218.5 / 6.3 78D164V (GAC→GTC) PP_2076hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (40/32);  new base A (2/4);  total (42/36)
Fisher's exact test for biased strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

GGGGCGTCGGAAGGCTGGATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTACTGGCTGTGCCATT  >  NC_002947/2363245‑2363533
                                                                                                                                             |                                                                                                                                                    
ggggCGTCGGAAGGCTGGATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAg                                                                                                                                              <  4:302095/149‑1 (MQ=255)
  ggCGTCGGAAGCCTGGATGCAGCGGGCGAGATCGATTGGGGGCAGGCGGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGCGCGGAACAATGGACGCAGCTCCTGGATGAC‑ATCCAgga                                                                                                                                            <  6:270685/149‑2 (MQ=21)
    cGTCGGAAGGCTGGATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCg                                                                                                                                          <  3:84910/149‑1 (MQ=255)
    cGTCGGAAGGCTGGATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCg                                                                                                                                          <  6:81541/149‑1 (MQ=255)
        ggAAGGCTGGATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTa                                                                                                                                        >  2:137608/1‑147 (MQ=255)
          aaGGCTGGATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAgg                                                                                                                                    <  2:190402/149‑1 (MQ=255)
             gCTGGATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATc                                                                                                                                                 >  5:138057/1‑133 (MQ=255)
             gCTGGATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATc                                                                                                                                                 <  6:138057/133‑1 (MQ=255)
              cTGGATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGAAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGa                                                                                                                                <  8:53159/149‑1 (MQ=255)
                ggATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGCGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATg                                                                                                                              >  4:187445/1‑149 (MQ=255)
                ggATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTa                                                                                                                                        >  2:144645/1‑139 (MQ=255)
                ggATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTa                                                                                                                                        <  1:144645/139‑1 (MQ=255)
                 gATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGTGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATg                                                                                                                              <  4:99745/148‑1 (MQ=255)
                 gATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATgg                                                                                                                             <  2:15771/149‑1 (MQ=255)
                 gATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATg                                                                                                                              <  7:249735/148‑1 (MQ=255)
                       gCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGGCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCCGCGGGGAAGGGCGGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGc                                                                                                                       <  8:123057/149‑1 (MQ=255)
                         gggCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGcc                                                                                                                      >  4:41828/1‑148 (MQ=255)
                          ggCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCa                                                                                                                         <  5:187968/144‑1 (MQ=255)
                          ggCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCa                                                                                                                         >  6:187968/1‑144 (MQ=255)
                                    aTTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAg                                                                                                          >  1:307772/1‑149 (MQ=255)
                                     ttGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAgg                                                                                                                                             <  3:83495/113‑1 (MQ=255)
                                     ttGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAgg                                                                                                                                             >  4:83495/1‑113 (MQ=255)
                                        tgGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAACCCGCGAAGGAtc                                                                                                      >  6:191385/1‑149 (MQ=255)
                                          ggCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGAtct                                                                                                     <  2:307772/148‑1 (MQ=255)
                                            cAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCAt                                                                                                  >  3:219206/1‑149 (MQ=255)
                                              ggCTGCTGCGGTATTCGTCGTCGAGCAGGCGTTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAAGGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATcc                                                                                                <  3:106077/149‑1 (MQ=255)
                                                   ctgcGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGAtt                                                                                           >  1:226925/1‑149 (MQ=255)
                                                     gcGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGc                                                                                         >  1:112609/1‑149 (MQ=255)
                                                      cGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGc                                                                                         <  2:112609/148‑1 (MQ=255)
                                                        gTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGACTATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCg                                                                                       <  1:263521/149‑1 (MQ=255)
                                                         tATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGgc                                                                                     >  5:186268/1‑149 (MQ=255)
                                                              gtcgtcgAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTgg                                                                                >  8:51977/1‑149 (MQ=255)
                                                               tcgtcgAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGGTGGACAATGGGCGCAGCTCCTGGAATTG‑AAACAGGTCGTAGACGGTGGTGGTACAGCCGAAACCGCGAAGGCTATCTAGCAGATTGCCGGCGCTtgg                                                                               >  2:312381/1‑149 (MQ=255)
                                                                        aGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTc                                                                      <  4:219206/149‑1 (MQ=255)
                                                                          gCGGTAGTTGTCGAGCATGCGCGGGGGCTGGGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCTAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTccc                                                                    <  1:312381/149‑1 (MQ=255)
                                                                             gTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAgg                                                                 <  1:137608/149‑1 (MQ=255)
                                                                              tAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAggg                                                                <  2:79950/149‑1 (MQ=255)
                                                                              tAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAggg                                                                <  2:226925/149‑1 (MQ=255)
                                                                                 ttGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGc                                                             >  5:290089/1‑149 (MQ=255)
                                                                                 ttGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGc                                                             <  6:290089/149‑1 (MQ=255)
                                                                                   gTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTcc                                                                     <  7:96229/139‑1 (MQ=255)
                                                                                   gTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTcc                                                                     >  8:96229/1‑139 (MQ=255)
                                                                                    tCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTc                                                          >  6:290049/1‑149 (MQ=255)
                                                                                    tCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCATAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTc                                                          >  3:37773/1‑149 (MQ=255)
                                                                                         cATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCgctgc                                                     <  3:187445/149‑1 (MQ=255)
                                                                                            gcgcGGGGTCAGCGGGGAAGGGGTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCgctgc                                                     >  7:205595/1‑146 (MQ=255)
                                                                                            gcgcGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCgctgc                                                     <  8:205595/146‑1 (MQ=255)
                                                                                                      aGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCCGGGCGTAGAGGGTGATGGTCCAGCCGAACCCCCGAAGGAAGTCAACAACATTCCCGCCCCTGGGGCCAGCATCCCAGGGGCCTTCCGTGAGGATGTAcaccgc                                        >  6:267910/1‑145 (MQ=16)
                                                                                                      aGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTAcagcag                                        >  8:269570/1‑149 (MQ=255)
                                                                                                            gAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGc                                   >  3:98588/1‑148 (MQ=255)
                                                                                                              aGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCAt                                >  2:4043/1‑149 (MQ=255)
                                                                                                               gggCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCAtt                               <  5:290049/149‑1 (MQ=255)
                                                                                                                cgCTGAACAATGGACGCAGCTCCTGGATCTC‑ATCCAGGACGTAGAGGCTGATCGTCCACCCGAAACCGCGAAGGAACTCATCCAGATTGCCGCCGCTGGCGGCAGGCTCACAGGGGGCGTCGCTGCTGAAGTCAAGCAGCGCGCCAttc                              >  4:203535/2‑148 (MQ=255)
                                                                                                                  cTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTggg                            <  3:41828/149‑1 (MQ=255)
                                                                                                                   tGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCGGATGTACAGCAGCGAGCCATTGGgc                           >  4:292323/1‑149 (MQ=255)
                                                                                                                    gAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGgcg                          >  1:61420/1‑149 (MQ=255)
                                                                                                                        aaTGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCAc                      >  3:320463/1‑149 (MQ=255)
                                                                                                                         aTGGATGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGGAGAAGTACAGCAGCCAGCCATTGGGCGCCAcc                     >  2:289199/1‑148 (MQ=25)
                                                                                                                         aTGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCAcg                     >  1:46687/1‑149 (MQ=255)
                                                                                                                          tGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCAcgc                    >  3:194747/1‑149 (MQ=255)
                                                                                                                          tGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCAcgc                    >  2:176630/1‑149 (MQ=255)
                                                                                                                            gACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCAcgcg                   <  7:51977/148‑1 (MQ=255)
                                                                                                                             aCGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCgctgc                                                     >  3:14225/1‑113 (MQ=255)
                                                                                                                             aCGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCgctgc                                                     <  4:14225/113‑1 (MQ=255)
                                                                                                                                 aGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCAcgcg                   >  3:237208/1‑143 (MQ=255)
                                                                                                                                 aGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCAcgcg                   <  4:237208/143‑1 (MQ=255)
                                                                                                                                 aGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTACTg             >  5:283837/1‑149 (MQ=255)
                                                                                                                                   gaccTGGATGAC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAggg                                                                >  5:318934/3‑96 (MQ=255)
                                                                                                                                   gaccTGGATGAC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAggg                                                                <  6:318934/94‑1 (MQ=255)
                                                                                                                                    tCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTACTGGCt          >  5:5170/1‑149 (MQ=255)
                                                                                                                                    tCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTACTGGCt          >  6:19904/1‑149 (MQ=255)
                                                                                                                                    tCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTACTGGCt          >  5:140278/1‑149 (MQ=255)
                                                                                                                                        ggATGAC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGctgctg                                                   <  5:207416/104‑1 (MQ=255)
                                                                                                                                        ggATGAC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGctgctg                                                   >  6:207416/1‑104 (MQ=255)
                                                                                                                                         gATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTACTgg            <  7:305872/142‑1 (MQ=255)
                                                                                                                                         gATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTACTgg            >  8:305872/1‑142 (MQ=255)
                                                                                                                                         gATGTC‑ATCCAGGTCATAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTACTGGCTGTGc      <  4:37773/148‑1 (MQ=255)
                                                                                                                                            gTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCCTTGGGCGCCACGCGGTAACGGCTGTGCCAtt  >  2:270845/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GGGGCGTCGGAAGGCTGGATGAAGCGGGCGATATCGATTGTGGGCAGGCTGCTGCGGTATTCGTCGTCGAGCAGGCGGTAGTTGTCGAGCATGCGCGGGGTCAGCGGGGAAGGGCTGAACAATGGACGCAGCTCCTGGATGTC‑ATCCAGGTCGTAGAGGGTGATGGTCCAGCCGAAACCGCGAAGGATCTCATCCAGATTGCCGGCGCTGGGGGCAGGCTCCCAGGGGGCGTCGCTGCTGATGTACAGCAGCGAGCCATTGGGCGCCACGCGGTACTGGCTGTGCCATT  >  NC_002947/2363245‑2363533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑