Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,389,141 T→C 5.4% M1T (ATG→ACG) † rlmKL → 23S rRNA (guanine(2445)‑N(2))/(guanine(2069)‑N(7))‑ methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,389,1410TC 5.4% 339.3 / 6.2 112M1T (ATG→ACG) †rlmKL23S rRNA (guanine(2445)‑N(2))/(guanine(2069)‑N(7))‑ methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (53/52);  new base C (3/3);  total (57/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

Only 100 of 114 total aligned reads displayed.

CGTACTGCCTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAGGTGCGCGAGCA  >  NC_002947/2388994‑2389285
                                                                                                                                                    |                                                                                                                                                
cGTACTGCCTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGAt                                                                                                                                                  <  3:249134/148‑1 (MQ=255)
cGTACTGCCTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGAt                                                                                                                                                  <  1:103847/148‑1 (MQ=255)
       ccTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGc                                                                                                                                           <  3:99135/148‑1 (MQ=255)
       ccTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGACGcat                                                                                                                                              >  3:242516/1‑143 (MQ=255)
       ccTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGACGcat                                                                                                                                              <  4:242516/145‑3 (MQ=255)
          gatgatCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCg                                                                                                                                       >  1:111608/1‑149 (MQ=255)
          gatgatCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCg                                                                                                                                       >  6:110189/1‑149 (MQ=255)
          gatgatCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTATGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCg                                                                                                                                       >  2:190232/1‑149 (MQ=255)
            tgatCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACTGGCAAACATCGCGGGGCGATGCGTCGCCCCgt                                                                                                                                      <  8:248354/148‑1 (MQ=255)
              atCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCgtca                                                                                                                                    <  7:246523/148‑1 (MQ=255)
               tCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGc                                                                                                                                                >  8:304916/1‑135 (MQ=255)
               tCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGc                                                                                                                                                <  7:304916/135‑1 (MQ=255)
                cTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCgtcagt                                                                                                                                  >  5:24978/1‑148 (MQ=255)
                   aGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTATGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCgtcag                                                                                                                                   <  1:190232/144‑1 (MQ=255)
                    gCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCaaa                                                                                                                              >  3:180852/1‑148 (MQ=255)
                        tCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGcc                                                                                                                         >  3:181187/1‑149 (MQ=255)
                        tCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGcc                                                                                                                         >  4:102946/1‑149 (MQ=255)
                          cTTGCCACTGGGCCAGGCACGGATCGGTCAGGCTGGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGAACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGcc                                                                                                                         >  1:39583/1‑148 (MQ=255)
                          cTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGccc                                                                                                                        >  2:197100/1‑148 (MQ=255)
                          cTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGATTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCgt                                                                                                                                      >  7:125652/1‑134 (MQ=255)
                            tGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAg                                                                                                                     >  1:176311/1‑149 (MQ=255)
                              ccACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAgg                                                                                                                    <  1:297274/148‑1 (MQ=255)
                               cACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCgtca                                                                                                                                    <  2:150183/131‑1 (MQ=255)
                               cACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCgtca                                                                                                                                    >  1:150183/1‑131 (MQ=255)
                               cACTCGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTATTCTGCAGCACGTTGTGTCTCGCAGAAGGACAACCGCACGCCTGCTGGAAAGGAGTACGTGCAAACATCGCGGGGCGAAGCGTCGCACACTCAGTCAAATAGACAAAGccg                                                                                                                  >  8:127553/1‑149 (MQ=255)
                                aCTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGcc                                                                                                                                          <  6:152262/124‑1 (MQ=255)
                                aCTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGcc                                                                                                                                          >  5:152262/1‑124 (MQ=255)
                                    ggCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTc                                                                                                                                             <  2:142826/117‑1 (MQ=255)
                                    ggCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTc                                                                                                                                             >  1:142826/1‑117 (MQ=255)
                                    ggCCAGGCACGGAGCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTg                                                                                                               <  7:114009/147‑1 (MQ=255)
                                     gCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCCCGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGaa                                                                                                            >  2:244037/1‑149 (MQ=255)
                                     gCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGaa                                                                                                            >  3:271550/1‑149 (MQ=255)
                                            aCGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCAt                                                                                                     >  3:105978/1‑149 (MQ=255)
                                                aTCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATggcc                                                                                                 >  6:238508/1‑146 (MQ=255)
                                                  cGGTCAGGC‑GGCCCGGCTTTTCTGCCGCACGGTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGa                                                                                               <  4:297129/149‑1 (MQ=255)
                                                   ggTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGAc                                                                                              <  3:255068/149‑1 (MQ=255)
                                                       aGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGtt                                                                                          <  2:304081/149‑1 (MQ=255)
                                                         gc‑ggcCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTc                                                                                        >  1:3539/1‑149 (MQ=255)
                                                         gc‑ggcCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTc                                                                                        <  1:219847/149‑1 (MQ=255)
                                                         gc‑ggcCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTc                                                                                        <  8:130551/149‑1 (MQ=255)
                                                            ggcCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGa                                                                                      <  4:181187/149‑1 (MQ=255)
                                                              cccGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAAc                                                                                    <  2:3539/149‑1 (MQ=255)
                                                                   cTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTAc                                                                               <  4:271550/149‑1 (MQ=255)
                                                                   cTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTAc                                                                               <  3:102946/149‑1 (MQ=255)
                                                                    ttttCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGACGCATCGCCCCgc                                                                                                                                      >  2:301701/1‑92 (MQ=255)
                                                                    ttttCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGACGCATCGCCCCgc                                                                                                                                      <  1:301701/93‑2 (MQ=255)
                                                                     tttCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTAcc                                                                              >  6:296718/1‑148 (MQ=255)
                                                                     tttCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCt                                                                             >  5:237623/1‑149 (MQ=255)
                                                                        cTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTc                                                                                                  <  3:164055/125‑1 (MQ=255)
                                                                        cTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTc                                                                                                  >  4:164055/1‑125 (MQ=255)
                                                                           cagcaCGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCt                                                                        <  2:172067/148‑1 (MQ=255)
                                                                              caCGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAACTAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTcc                                                                    <  5:208975/149‑1 (MQ=255)
                                                                              caCGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTcc                                                                    >  3:142197/1‑149 (MQ=255)
                                                                                 gTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCa                                                                  >  8:225085/1‑148 (MQ=255)
                                                                                    gtgtgtCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAgg                                                              >  5:33128/1‑149 (MQ=255)
                                                                                    gtgtgtCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAg                                                               <  4:180852/148‑1 (MQ=255)
                                                                                     tgtgtCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGc                                                             >  4:148362/1‑149 (MQ=255)
                                                                                     tgtgtCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGc                                                             <  6:24978/149‑1 (MQ=255)
                                                                                         tCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCtt                                                          <  5:296718/148‑1 (MQ=255)
                                                                                         tCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCtt                                                          >  1:98215/1‑148 (MQ=255)
                                                                                         tCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCtt                                                          <  4:276805/148‑1 (MQ=255)
                                                                                           gCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGa                                                        <  2:98215/148‑1 (MQ=255)
                                                                                            cAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATATCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGaaa                                                      <  4:167954/149‑1 (MQ=255)
                                                                                            cAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACttt                                                                                 >  5:172369/1‑122 (MQ=255)
                                                                                            cAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGa                                                        >  5:55983/1‑147 (MQ=255)
                                                                                            cAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGGCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACttt                                                                                 <  6:172369/122‑1 (MQ=255)
                                                                                                 ggCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTg                                                 <  7:68947/149‑1 (MQ=255)
                                                                                                    cACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTc                                              >  2:289171/1‑149 (MQ=255)
                                                                                                    cACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTc                                              >  1:151051/1‑149 (MQ=255)
                                                                                                       ccGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAg                                                               >  1:204710/1‑129 (MQ=255)
                                                                                                          cACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGaa                                        <  7:225085/149‑1 (MQ=255)
                                                                                                             gCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGTGAGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCTGACCGTTTCGAACTTTACATCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAagat                                     <  6:63548/149‑2 (MQ=255)
                                                                                                             gCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAagag                                     >  2:213823/1‑149 (MQ=255)
                                                                                                             gCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGCCCCAAAGGCCTTCAAAGCCTGCTCGCCGAagag                                     >  2:187758/1‑149 (MQ=255)
                                                                                                                tgctgGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGc                                   >  6:15741/1‑148 (MQ=255)
                                                                                                                    ggAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAgg                              >  2:311874/1‑149 (MQ=255)
                                                                                                                     gAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAggg                             >  4:294191/1‑149 (MQ=255)
                                                                                                                     gAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAggg                             >  4:53397/1‑149 (MQ=255)
                                                                                                                     gAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAggg                             >  3:102048/1‑149 (MQ=255)
                                                                                                                          gtgtACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCg                        <  3:53397/149‑1 (MQ=255)
                                                                                                                          gtgtACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCg                        <  2:176311/149‑1 (MQ=255)
                                                                                                                          gtgtACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGCCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCg                        <  1:187758/149‑1 (MQ=255)
                                                                                                                                gggCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGCACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTg                  >  3:255108/1‑149 (MQ=255)
                                                                                                                                gggCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCtt                   >  5:69324/1‑148 (MQ=255)
                                                                                                                                gggCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCtt                   >  6:43754/1‑148 (MQ=255)
                                                                                                                                gggCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTg                  <  4:142197/149‑1 (MQ=255)
                                                                                                                                  gCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGAc                <  6:237623/149‑1 (MQ=255)
                                                                                                                                      gactgacgGGGCGACGCATCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGc                                   >  7:99094/7‑126 (MQ=255)
                                                                                                                                      aCATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAgg            >  3:81228/1‑149 (MQ=255)
                                                                                                                                       cATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGGGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAGGt           <  5:181883/149‑1 (MQ=255)
                                                                                                                                        aTCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAGGTg          >  8:76354/1‑149 (MQ=255)
                                                                                                                                         tCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAg                               <  4:33983/127‑1 (MQ=255)
                                                                                                                                         tCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAg                               >  3:33983/1‑127 (MQ=255)
                                                                                                                                            cgGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAGGTGcgcg      <  3:148362/149‑1 (MQ=255)
                                                                                                                                                gCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAGGTGCGCGAGca  <  4:25781/149‑1 (MQ=255)
                                                                                                                                                gCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAGGTGCGCGAGca  <  3:294191/149‑1 (MQ=255)
                                                                                                                                                 cGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAGGt           >  4:175249/1‑139 (MQ=255)
                                                                                                                                                 cGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAGGt           <  3:175249/139‑1 (MQ=255)
                                                                                                                                                  gATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAaga                                      >  7:271634/1‑111 (MQ=255)
                                                                                                                                                  gATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAaga                                      <  8:271634/111‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
CGTACTGCCTGATGATCTAAGCTGTCCTTGCCACTGGGCCAGGCACGGATCGGTCAGGC‑GGCCCGGCTTTTCTGCAGCACGTTGTGTGTCGCAGAAGGCCACCCGCACGCCTGCTGGAAAGGTGTACGGGCAAACATCGCGGGGCGATGCGTCGCCCCGTCAGTCAAATAGCCCAAGGCGCTGGAATACTCATGTCGGACCGTTTCGAACTTTACCTCACCTGTCCCAAAGGCCTTGAAAGCCTGCTCGCCGAAGAGGCCAAGGGCCTCGGCCTTGACGAGGTGCGCGAGCA  >  NC_002947/2388994‑2389285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑