Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 39,564 C→T 7.5% P98L (CCA→CTA)  PP_0036 → transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294739,5640CT 7.5% 153.2 / 2.2 53P98L (CCA→CTA) PP_0036transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (27/22);  new base T (2/2);  total (29/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.89e-01

CCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACATCGTAAACCGCGATCTAGTCGCCTACGTGCGTGATCATGCCGAAATGCAGCTGGAAATCCATTCAGAAT  >  NC_002947/39425‑39710
                                                                                                                                           |                                                                                                                                                  
ccAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGcc                                                                                                                                            <  8:195602/148‑1 (MQ=255)
  aGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTg                                                                                                                                         <  2:143058/149‑1 (MQ=255)
    tGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGc                                                                                                                                       <  3:162964/149‑1 (MQ=255)
             tGCGGAAACGCTTGGAGAATCTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCAACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGACAGGGTCAGCCATAAACAGTGCCAGCTCTACGGCCCACACGGCCTTGGCAAGTCCGGt                                                                                                                              >  3:30837/1‑149 (MQ=255)
             tGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGt                                                                                                                              >  6:254680/1‑149 (MQ=255)
               cGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTTGGCCCAGACGGCCTTGGCAAGTCCGGTg                                                                                                                             <  5:319163/148‑1 (MQ=255)
               cGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTg                                                                                                                             <  3:33128/148‑1 (MQ=255)
                 gAAACGCTTGGAGAAACTGGAAGATCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCATGACCCGCACAGGCGAGAGGGTCAGCCATAACCTGAGCCAGCTATTCGTCCCAGACGGCCTTGGCAAGTCCGTTGaa                                                                                                                           >  3:164533/1‑148 (MQ=255)
                       cTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAgg                                                                                                                       >  6:208371/1‑146 (MQ=255)
                       cTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAgg                                                                                                                       <  5:208371/146‑1 (MQ=255)
                        ttGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGt                                                                                                                   >  5:84542/1‑149 (MQ=255)
                        ttGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGt                                                                                                                   >  4:211806/1‑149 (MQ=255)
                          ggAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGt                                                                                                                   <  3:211806/147‑1 (MQ=255)
                             gaAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTgg                                                                                                                                        <  7:192265/123‑1 (MQ=255)
                             gaAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTgg                                                                                                                                        >  8:192265/1‑123 (MQ=255)
                                  tGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCt                                                                                                          >  5:261737/1‑148 (MQ=255)
                                            caTTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGa                                                                                               >  6:190392/1‑149 (MQ=255)
                                             aTTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCTAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATcgc                                                                                                 <  7:271419/146‑1 (MQ=255)
                                             aTTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCTAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCACCAGGCTTACCATcgc                                                                                                 >  8:271419/1‑146 (MQ=255)
                                              ttGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATc                                                                                             <  5:254680/149‑1 (MQ=255)
                                                ggTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATca                                                                                            <  6:84542/148‑1 (MQ=255)
                                                    ttgttgtgtTTGTAGACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACagag                                                                                       >  5:21251/6‑149 (MQ=255)
                                                      cccGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACag                                                                                         >  1:10624/1‑145 (MQ=255)
                                                      cccGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACag                                                                                         <  2:10624/145‑1 (MQ=255)
                                                       ccGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCTAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGc                                                                                      >  4:162365/1‑147 (MQ=255)
                                                       ccGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGc                                                                                      >  5:168615/1‑147 (MQ=255)
                                                         gtgtTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGt                                                                                  >  3:303177/1‑149 (MQ=255)
                                                         gtgtTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTACCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGt                                                                                  >  1:306808/1‑149 (MQ=255)
                                                           gtTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGc                                                                                >  3:34301/1‑149 (MQ=255)
                                                                  tACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGt                                                                                  >  1:287508/1‑140 (MQ=255)
                                                                  tACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGt                                                                                  <  2:287508/140‑1 (MQ=255)
                                                                     aaaCACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACAt                                                                      >  3:95652/1‑149 (MQ=255)
                                                                     aaaCACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACAt                                                                      >  5:77664/1‑149 (MQ=255)
                                                                     aaaCACAACAAGCTTGCCCTGACCCGCACACGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACAt                                                                      <  2:166069/149‑1 (MQ=255)
                                                                          caacaaGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTg                                                                                                                                         <  3:161492/77‑1 (MQ=255)
                                                                          caacaaGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTg                                                                                                                                         >  4:161492/1‑77 (MQ=255)
                                                                                gCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCATGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACATCGTAAACcgc                                                            >  6:21286/1‑148 (MQ=255)
                                                                                          aCCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCTAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACATCGTAAACCGCGATCTAGTc                                                   <  3:162365/147‑1 (MQ=255)
                                                                                          aCCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACATCGTAAACCGCGATCTAGTc                                                   <  2:324543/147‑1 (MQ=255)
                                                                                          aCCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACATCGTAAACCGCGATCTAGTc                                                   <  6:168615/147‑1 (MQ=255)
                                                                                          aCCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACATCGTAAACCGCGATCTAGTc                                                   <  2:319479/147‑1 (MQ=255)
                                                                                                      gagaGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACATCGTAAACCGCGATCTAGTCGCCTACGTGCGTGa                                     <  4:95652/149‑1 (MQ=255)
                                                                                                      gagaGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACATCGTAAACCGCGATCTAGTCGCCTACGTGCGTGa                                     <  6:77664/149‑1 (MQ=255)
                                                                                                           ggTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACATCGTAAACCGCGATCTAGTCGCCTACGTGCGTGATCATg                                >  1:132137/1‑149 (MQ=255)
                                                                                                             tCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACAATCGCGATCACAGAGCCAGTGCTGAACGACATCGTAAACCGCGATCTAGTCGCCTACGTGCGTGATCATGcc                              >  7:82119/1‑149 (MQ=255)
                                                                                                                    ttACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACATCGTAAACCGCGATCTAGTCGCCTACGTGCGTGATCATGCCGAAATg                        >  2:218779/1‑148 (MQ=255)
                                                                                                                        cTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACATCGTAAACCGCGATCTAGTCGCCTACGTGCGTGATCATGCCGAAATGCAGCt                   >  3:192345/1‑149 (MQ=255)
                                                                                                                                 ctctTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACATCGTAAACCGCGATCTAGTCGCCTACGTGCGTGATCATGCCGAAATGCAGCTGGAAATCCa          <  2:306808/149‑1 (MQ=255)
                                                                                                                                 ctctTCGGCCCAGACGGCCTTGGAAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACATCGTAAACCGCGATCTAGTCGCCTACGTGCGTGATCATGCCGAAATGCAGCTGGAAATCCa          >  2:191400/1‑149 (MQ=255)
                                                                                                                                 ccctTCCGGCCACACGGCCTTGGCATCTCCGGTGAAATGCAAGTCCCCAGGCTAACCATCGCGATCACAGAGCCAGTGCTGACCGACATCGTAAACCGCGATCTACTCGCCTACGTGCGTCATCATGCCGAAATGCACCAGGAAATCCa          <  4:30837/147‑1 (MQ=255)
                                                                                                                                  tctTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACATCGTAAACCGCGATCTAGTCGCCTACGTGCGTGATCATGCCGAAATGCAGCTGGAAATCCAt         >  5:312481/1‑149 (MQ=255)
                                                                                                                                   ctTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACATCGTAAACCGCGATCTAGTCGCCTACGTGCGTGATCATGCCGAAATGCAGCTGGAAATCCAtt        >  5:293946/1‑149 (MQ=255)
                                                                                                                                    ttCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACATCGTAAACCGCGATCTAGTCGCCTACGTGCGTGATCATGCCGAAATGCAGCTGGAAATCCAt         <  5:190392/147‑1 (MQ=255)
                                                                                                                                         cccAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACATCGTAAACCGCGATCTAGTCGCCTACGTGCGTGATCATGCCGAAATGCAGCTGGAAATCCATTCAGAAt  <  1:191400/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                  
CCAGTGGTAGGCCTGCGGAAACGCTTGGAGAAACTGGAAGAGCACATTGGTGCTCCCGTGTTTGTCTACAAACACAACAAGCTTGCCCTGACCCGCACAGGCGAGAGGGTCAGCCATTACCTGTGCCAGCTCTTCGGCCCAGACGGCCTTGGCAAGTCCGGTGAAAAGGAAGTCCCCAGGCTTACCATCGCGATCACAGAGCCAGTGCTGAACGACATCGTAAACCGCGATCTAGTCGCCTACGTGCGTGATCATGCCGAAATGCAGCTGGAAATCCATTCAGAAT  >  NC_002947/39425‑39710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑