BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,404,559	A→T	7.3%	I396F (ATC→TTC) ‡	PP_2106 →	ammonium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,404,559	0	A	T	7.3%	370.8 / 14.7	123	I396F (ATC→TTC) ‡	PP_2106	ammonium transporter
Reads supporting (aligned to +/- strand): ref base A (60/54); new base T (2/7); total (62/61)
Fisher's exact test for biased strand distribution p-value = 9.51e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

Only 100 of 123 total aligned reads displayed.

GGTCAGCCAGTTGCTGGGCAGCCTGATGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCTGAATGTCAACTGTTCGGCACCCTCGGCTGCCACCTCTG  >  NC_002947/2404414‑2404702

ggTCAGCCAGTTGCTGGGCAGCCTGATGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCg                                                                                                                                              >  2:227736/1‑149 (MQ=255)

 gTCAGCCAGTTGCTGGGCAGCCTGATGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCgg                                                                                                                                             >  3:300744/1‑149 (MQ=255)

  gcAGCCAGTTGCTGGGCAGCCTGATGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGt                                                                                                                                            <  1:117473/148‑1 (MQ=255)

      ccAGTTGCTGGGCAGCCTGATGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTa                                                                                                                                        >  3:36378/1‑149 (MQ=255)

      ccAGTTGCTGGGCAGCCTGATGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTa                                                                                                                                        >  6:247664/1‑149 (MQ=255)

      ccAGTTGCTGGGCAGCCTGATGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGATCCGGTGcag                                                                                                                                        <  3:131805/149‑3 (MQ=255)

      ccAGTTGCTGGGCAGCCTGATGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGATCCGGTGcag                                                                                                                                        <  2:317214/149‑3 (MQ=255)

      ccAGTTGCTGGGCAGCCTGATGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGATCCGGTGcag                                                                                                                                        >  4:131805/1‑147 (MQ=255)

      ccAGTTGCTGGGCAGCCTGATGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGCAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTa                                                                                                                                        >  4:93280/1‑149 (MQ=255)

       cAGTTGCTGGGCAGCCTGATGGGCGTGCTGGTGGCGTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGATCCGGTGcagc                                                                                                                                       <  8:294701/149‑4 (MQ=255)

       cAGTTGCTGGGCAGCCTGATGGGCGTGCTGGTGGCCTTGGCTGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGATCCGGTGcagc                                                                                                                                       <  1:83781/149‑4 (MQ=255)

                ggCAGCCTGATGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTAccagccag                                                                                                                                <  1:229324/147‑1 (MQ=255)

                ggCAGCCTGATGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGa                                                                                                                              <  4:36378/149‑1 (MQ=255)

                  cAGCCTGATGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGAtt                                                                                                                            <  5:89104/149‑1 (MQ=255)

                  cAGCCTGATGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGAtt                                                                                                                            <  5:256090/149‑1 (MQ=255)

                    gCCTGATGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGa                                                                                                                          <  6:210226/149‑1 (MQ=255)

                     ccTGATGGGCGTGCTTGTGGCCTTGGCCGGTGGATTTGCCGTTTACGGAGCCATTCGTGCGCTGCATGGCCTT‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTGCCACCGGATCGGTGCTACCAGCCAGGATTGa                                                                                                                          <  1:192951/149‑1 (MQ=255)

                     ccTGATGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAg                                                                                                                         >  6:65624/1‑149 (MQ=255)

                     ccTGATGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAg                                                                                                                         >  6:77788/1‑149 (MQ=255)

                      cTGATGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGc                                                                                                                        >  4:306010/1‑149 (MQ=255)

                         aTGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCcag                                                                                                                     <  5:65624/149‑1 (MQ=255)

                          tGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCcag                                                                                                                     <  2:145864/148‑1 (MQ=255)

                          tGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCcag                                                                                                                     <  2:150823/148‑1 (MQ=255)

                           gggCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGATCCGGTGcag                                                                                                                                        <  8:121756/128‑3 (MQ=255)

                           gggCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGATCCGGTGcag                                                                                                                                        >  7:121756/1‑126 (MQ=255)

                               gTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCcaggtcagg                                                                                                               <  3:77862/149‑1 (MQ=255)

                                tGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCcaggtcaggt                                                                                                              <  5:127696/149‑1 (MQ=255)

                                   tggtggCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTg                                                                                                             >  1:276086/1‑147 (MQ=255)

                                     gtggCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAgg                                                                                                         <  2:54405/149‑1 (MQ=255)

                                      tggCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAggg                                                                                                        >  4:59486/1‑149 (MQ=255)

                                       ggCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGt                                                                                                       <  1:310930/149‑1 (MQ=255)

                                        gCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTg                                                                                                      <  3:301580/149‑1 (MQ=255)

                                         ccTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTgg                                                                                                     <  2:23639/149‑1 (MQ=255)

                                          cTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTggg                                                                                                    >  6:302693/1‑149 (MQ=255)

                                          cTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTggg                                                                                                    >  1:24266/1‑149 (MQ=255)

                                          cTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTgg                                                                                                     <  5:89895/148‑1 (MQ=255)

                                          cTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTgg                                                                                                     >  6:89895/1‑148 (MQ=255)

                                           tttgCCGGTGCATTTGCAGTGTACGGAGCAATTCGAGCGCTCCATGTACAG‑CGCTAGAGCCATGAGCAGGAGTTACAGGGCCCGGACCTGTCGCTG‑CCCCGGATCGGTGCTACCAGCCAGGATTGAGCCAGTACAGGTGCAGGTTGTGc                                                                                                   >  2:233627/4‑149 (MQ=255)

                                           ttGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTAccagccag                                                                                                                                >  8:51316/1‑120 (MQ=255)

                                           ttGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTAccagccag                                                                                                                                <  7:51316/120‑1 (MQ=255)

                                           ttGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGc                                                                                                   >  4:203901/1‑149 (MQ=255)

                                           ttGGCCGGTGGATTTGCCATGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGATCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGc                                                                                                   >  4:300156/1‑149 (MQ=255)

                                                 ggTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGt                                                                                                 >  3:187700/1‑145 (MQ=255)

                                                 ggTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGt                                                                                                 <  4:187700/145‑1 (MQ=255)

                                                  gTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCcaggtcagg                                                                                                               <  4:157199/130‑1 (MQ=255)

                                                  gTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCcaggtcagg                                                                                                               >  3:157199/1‑130 (MQ=255)

                                                  gTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGgcgc                                                                                            >  2:24966/1‑149 (MQ=255)

                                                     gATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGgcgcgc                                                                                          <  7:81727/148‑1 (MQ=255)

                                                       tttGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGcc                                                                                         >  1:155334/1‑147 (MQ=255)

                                                         tGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGGGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAg                                                                                      >  1:229297/1‑148 (MQ=255)

                                                           ccGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGaa                                                                                    <  4:300744/148‑1 (MQ=255)

                                                             gtgtACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAg                                                                                 <  2:224437/149‑1 (MQ=255)

                                                              tgtACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAgg                                                                                <  3:59486/149‑1 (MQ=255)

                                                              tgtACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAgg                                                                                <  5:77788/149‑1 (MQ=255)

                                                               gtACGGAGCCATTCGTGCGCTGCATGGCCTGACGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAgg                                                                                >  1:34514/1‑149 (MQ=255)

                                                                  cGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTc                                                                            >  2:99059/1‑149 (MQ=255)

                                                                   ggAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCt                                                                           <  2:276086/149‑1 (MQ=255)

                                                                     aGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCtgt                                                                         <  4:151461/149‑1 (MQ=255)

                                                                        cATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTa                                                                      >  4:60171/1‑149 (MQ=255)

                                                                         aTTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTAt                                                                     <  4:137564/149‑1 (MQ=255)

                                                                             gTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGcc                                                                  >  8:101620/1‑148 (MQ=255)

                                                                             gTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGcc                                                                  >  5:116765/1‑148 (MQ=255)

                                                                              tGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATGGGCTTCTGTGTATGCCaa                                                                >  5:283822/1‑149 (MQ=255)

                                                                                cgcTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACGGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAAc                                                               <  5:247664/148‑1 (MQ=255)

                                                                                cgcTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTAc                                                                                                                                       <  5:87405/76‑1 (MQ=255)

                                                                                         gCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTCCCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTCTGCCAACCCGGGACCTg                                                     >  3:189274/1‑149 (MQ=255)

                                                                                            tG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCca                                                  >  3:124349/1‑149 (MQ=255)

                                                                                            tG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCca                                                  >  4:62866/1‑149 (MQ=255)

                                                                                            tG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGCACCTGCca                                                  >  8:174780/1‑149 (MQ=255)

                                                                                               cgcTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCca                                                  <  3:93280/147‑1 (MQ=255)

                                                                                                  ttGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACAtgc                                             >  7:11518/1‑149 (MQ=255)

                                                                                                     aGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACAtgctgc                                          <  2:34514/149‑1 (MQ=255)

                                                                                                     aGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACAtgctgc                                          <  3:203901/149‑1 (MQ=255)

                                                                                                       ccATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGTTTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCt                                        >  2:22017/1‑149 (MQ=255)

                                                                                                       ccATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCt                                        >  6:194987/1‑149 (MQ=255)

                                                                                                       ccATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCt                                        <  6:116765/149‑1 (MQ=255)

                                                                                                       ccATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCt                                        >  2:114210/1‑149 (MQ=255)

                                                                                                         aTGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCTg                                       >  1:137180/1‑148 (MQ=255)

                                                                                                          tGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCTGaa                                     >  2:205827/1‑149 (MQ=255)

                                                                                                          tGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCTGaa                                     <  1:98456/149‑1 (MQ=255)

                                                                                                            aGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCTGAAt                                    <  2:285882/148‑1 (MQ=255)

                                                                                                            aGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCTGAATg                                   >  2:185243/1‑149 (MQ=255)

                                                                                                                 gAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCTGAATGTCaa                               <  4:236620/148‑1 (MQ=255)

                                                                                                                    ttCCAGGGAGCGGACCTGTCGCTG‑CACCGGATCGGTAATAACAGCAAGGATTTAGCCAGGTCAGGTGCAGGGTGGGAGTGGCTCGACTAGAATAGGCTTAAGTGTATGCAAACCCGGGACCAGCAACATCCTGCCTGAATGTAAACtt                            >  2:40344/1‑147 (MQ=255)

                                                                                                                       cAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACAtgctgc                                          >  3:223910/1‑131 (MQ=255)

                                                                                                                       cAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACAtgctgc                                          <  4:223910/131‑1 (MQ=255)

                                                                                                                       cAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCTGAATGTCAACTGTTc                         <  5:112695/148‑1 (MQ=255)

                                                                                                                       cAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCTGAATGTCAACTGTTCg                        >  4:122623/1‑149 (MQ=255)

                                                                                                                        aGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCTGAATGTCAACTGTTCgg                       >  6:29822/1‑149 (MQ=255)

                                                                                                                         gggCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGCGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCTGAATGTCaa                               <  3:276385/140‑1 (MQ=255)

                                                                                                                         gggCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGCGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCTGAATGTCaa                               >  4:276385/1‑140 (MQ=255)

                                                                                                                                gACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCTGAATGTCAACTGTTCGGCACCCTCg               >  8:24561/1‑149 (MQ=255)

                                                                                                                                 aCCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTCTATGCCAACCCGGGACCTGCCACATGCTGCCTGAATGTCAACTGTTCGGCACCCTCgg              >  5:273311/1‑149 (MQ=255)

                                                                                                                                   cTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCTGAATGTCAACTGTTCGGCACCCTCGGCt            <  2:155334/149‑1 (MQ=255)

                                                                                                                                   cTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCTGAATGTCAACTGTTCGGCACCCTCGGCt            >  7:1747/1‑149 (MQ=255)

                                                                                                                                    tGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACAtgc                                             >  1:195447/1‑115 (MQ=255)

                                                                                                                                           g‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACAtg                                              <  3:280066/107‑1 (MQ=255)

                                                                                                                                           g‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACAtg                                              >  4:280066/1‑107 (MQ=255)

                                                                                                                                             cACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGCACCTGCCACATGCTGCCTGAATGTCAACTGTTCGGCACCCTCGGCTGCCACctct   <  7:174780/149‑1 (MQ=255)

                                                                                                                                              aCCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCTGAATGTCAACTGTTCGGCACCCTCGGCTGCCACCTCTg  >  2:260222/1‑149 (MQ=255)

GGTCAGCCAGTTGCTGGGCAGCCTGATGGGCGTGCTGGTGGCCTTGGCCGGTGGATTTGCCGTGTACGGAGCCATTCGTGCGCTGCATGGCCTG‑CGCTTGAGCCATGAGCAGGAGTTCCAGGGCGCGGACCTGTCGCTG‑CACCGGATCGGTGCTACCAGCCAGGATTGAGCCAGGTCAGGTGCAGGGTGGGCGTGGCGCGCCTAGAATAGGCTTCTGTGTATGCCAACCCGGGACCTGCCACATGCTGCCTGAATGTCAACTGTTCGGCACCCTCGGCTGCCACCTCTG  >  NC_002947/2404414‑2404702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`