BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,405,349	G→A	5.2%	V116M (GTG→ATG) ‡	PP_2108 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,405,349	0	G	A	5.2%	359.3 / 4.0	117	V116M (GTG→ATG) ‡	PP_2108	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (52/58); new base A (4/2); total (56/61)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.87e-01

Only 100 of 118 total aligned reads displayed.

TTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAGTGTCGAGGT  >  NC_002947/2405202‑2405494

ttGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCTGCGGACTTCACGGTGATCGACGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGt                                                                                                                                                   <  5:248772/149‑1 (MQ=255)

ttGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGt                                                                                                                                                   >  6:277805/1‑149 (MQ=255)

ttGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGt                                                                                                                                                   <  5:69498/149‑1 (MQ=255)

ttGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGt                                                                                                                                                   <  6:141787/149‑1 (MQ=255)

ttGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGt                                                                                                                                                   <  5:253268/149‑1 (MQ=255)

gctaTCGGAGGCGCCACCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGt                                                                                                                                                   >  8:229918/4‑149 (MQ=255)

  gATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTg                                                                                                                                                  >  2:218306/1‑148 (MQ=255)

        tGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGt                                                                                                                                           <  5:259931/149‑1 (MQ=255)

        tGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGt                                                                                                                                           >  1:192815/1‑149 (MQ=255)

          gcgcCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCg                                                                                                                                         <  5:277805/149‑1 (MQ=255)

                 cGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGCACCCATCTGTCgaattcc                                                                                                                                   <  1:294365/148‑7 (MQ=255)

                      gCCAGCACGTGCAAGGGCTGGCGGCTGACTTCACGGTGATCGAGTTCAGCCCGAGCGA‑GTCCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGAGCgg                                                                                                                              <  5:99713/148‑1 (MQ=255)

                      gCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGc                                                                                                                             <  1:200046/149‑1 (MQ=255)

                       ccAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCa                                                                                                                            >  2:88968/1‑149 (MQ=255)

                        cAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GCCCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCAc                                                                                                                           >  8:42168/1‑149 (MQ=255)

                            aCGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCAcgccg                                                                                                                       <  4:115473/149‑1 (MQ=255)

                            aCGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCAcgccg                                                                                                                       >  3:11878/1‑149 (MQ=255)

                                gCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGGGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCgca                                                                                                                   <  4:81392/149‑1 (MQ=255)

                                gCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCcgcg                                                                                                                     >  1:242869/1‑148 (MQ=255)

                                gCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCcgcg                                                                                                                     >  7:242756/1‑147 (MQ=255)

                                gCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCcgcg                                                                                                                     >  4:95/1‑147 (MQ=255)

                                 cAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCgcag                                                                                                                  <  4:6237/149‑1 (MQ=255)

                                  aaGGGCTGTCGGCGTAATTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCgcagg                                                                                                                 <  8:257155/149‑1 (MQ=255)

                                        tGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTg                                                                                                           >  1:172647/1‑149 (MQ=255)

                                          gcggcgGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGATTCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCt                                                                                                         >  8:140519/1‑149 (MQ=255)

                                          gcggcgGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTg                                                                                                           <  2:253324/147‑1 (MQ=255)

                                          gcggcgGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCt                                                                                                         >  5:29859/1‑149 (MQ=255)

                                                gACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCAt                                                                                                   <  2:172647/149‑1 (MQ=255)

                                                   ttCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATgcg                                                                                                <  8:242756/149‑1 (MQ=255)

                                                   ttCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATgcg                                                                                                >  3:86116/1‑149 (MQ=255)

                                                         gTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAg‑g                                                                                         <  7:229918/149‑1 (MQ=255)

                                                         gTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAg‑g                                                                                         <  4:11878/149‑1 (MQ=255)

                                                            aTCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAg                                                                                      >  3:147142/1‑149 (MQ=255)

                                                              cGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCgcaggcag                                                                                                              <  6:135128/124‑1 (MQ=255)

                                                               gAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGCCCATGCGCAAAG‑GCAGCg                                                                                    >  6:230991/1‑148 (MQ=255)

                                                               gAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGccc                                                                                   >  7:56536/1‑147 (MQ=255)

                                                               gAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCgg                                                                                   >  3:116709/1‑149 (MQ=255)

                                                                        ccGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGc                                                                          >  6:145274/1‑149 (MQ=255)

                                                                         cgcgcgA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGc                                                                                     >  8:60871/1‑137 (MQ=255)

                                                                         cgcgcgA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGc                                                                                     <  7:60871/137‑1 (MQ=255)

                                                                          gcgcgA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCgg                                                                        <  1:218306/149‑1 (MQ=255)

                                                                            gcgA‑GACCGTCCGGCGAATCAGCAAAAGTTCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAg                                                                                           <  8:270009/129‑1 (MQ=255)

                                                                            gcgA‑GACCGTCCGGCGAATCAGCAAAAGTTCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAg                                                                                           >  7:270009/1‑129 (MQ=255)

                                                                            gcgA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCgggg                                                                      <  4:147142/149‑1 (MQ=255)

                                                                                    cGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAAt                                                               >  2:33763/1‑149 (MQ=255)

                                                                                        cGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAg                                                           <  5:145274/149‑1 (MQ=255)

                                                                                         ggCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCg                                                                         >  5:246956/1‑134 (MQ=255)

                                                                                         ggCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCg                                                                         <  6:246956/134‑1 (MQ=255)

                                                                                          gCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAgcg                                                         <  1:33763/149‑1 (MQ=255)

                                                                                          gCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAgcg                                                         >  3:24099/1‑149 (MQ=255)

                                                                                          gCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAgcg                                                         >  3:28239/1‑149 (MQ=255)

                                                                                            gAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCa                                                       >  4:258396/1‑149 (MQ=255)

                                                                                             aaTCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCAt                                                      <  2:192815/149‑1 (MQ=255)

                                                                                                cAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGt                                                                                                                                       <  1:188835/66‑1 (MQ=255)

                                                                                                cAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGt                                                                                                                                       >  2:188835/1‑66 (MQ=255)

                                                                                                cAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGa                                                   >  4:282991/1‑149 (MQ=255)

                                                                                                cAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGa                                                   >  6:163024/1‑149 (MQ=255)

                                                                                                  gCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACg                                                 >  8:169726/1‑149 (MQ=255)

                                                                                                  gCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACg                                                 >  7:181173/1‑149 (MQ=255)

                                                                                                   cAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACgg                                                <  5:299706/149‑1 (MQ=255)

                                                                                                     aaaGTGCGGTGCCCTTCGATCAGCTGATGCTGTAATTCGACAGATGGCTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGGTGACCATGCGCAAAG‑GGAGCGGCTACCTGGCGTGGCTGCAATGAAGCTCATTGAc                                                  <  7:84801/145‑1 (MQ=255)

                                                                                                     aaaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGgca                                              <  5:163024/149‑1 (MQ=255)

                                                                                                     aaaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGgca                                              <  4:86116/149‑1 (MQ=255)

                                                                                                     aaaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGgca                                              <  3:282991/149‑1 (MQ=255)

                                                                                                     aaaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGgca                                              <  4:116709/149‑1 (MQ=255)

                                                                                                     aaaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGAATCGGTCGGTGACGCGCGGCACGCCCCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGATGCAATGAAGCGAATTGACgag                                               >  8:84801/1‑146 (MQ=255)

                                                                                                      aaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGgcag                                             <  7:42168/149‑1 (MQ=255)

                                                                                                      aaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGgc                                               <  1:316988/147‑1 (MQ=255)

                                                                                                      aaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGgc                                               >  2:316988/1‑147 (MQ=255)

                                                                                                          gCGGAGCCCTTCGATCAGCTGAACCTGGAATTCGACAGATCGGTGCATCTGTCGGAGACGCGCGGCACGCCGCGCACGCAGGTGCTGACCATGCGCAAAG‑GCAGAGGCTACCTCGCGCGGCTGCAATGAAACGCATTGACGGCAGCAAg                                         >  6:270134/1‑149 (MQ=255)

                                                                                                           cGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGTAGCAAgg                                        >  6:253139/1‑149 (MQ=255)

                                                                                                           cGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAgg                                        <  6:96048/149‑1 (MQ=255)

                                                                                                           cGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAg                                         <  7:169726/148‑1 (MQ=255)

                                                                                                                cccTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGAt                                    <  4:28239/148‑1 (MQ=255)

                                                                                                                cccTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGAt                                    <  4:24099/148‑1 (MQ=255)

                                                                                                                 ccTTCGATCAGCTGATCCTGGAAATCGACAGCTGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAATCGCATTGACGGCAGCAAGGGGATGt                                  <  4:55596/149‑1 (MQ=255)

                                                                                                                  cTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGtt                                 >  7:207830/1‑149 (MQ=255)

                                                                                                                  cTTCGATCAGCTGATCCTGCAATTCGTCAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGTGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGtt                                 <  2:239812/149‑1 (MQ=255)

                                                                                                                   ttCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCgggg                                                                      >  8:30322/1‑111 (MQ=255)

                                                                                                                   ttCCATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCACGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATCAAGCCCAATGACGGCAGCAAGGGGAAGTTc                                >  8:175381/1‑149 (MQ=255)

                                                                                                                     cGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTc                                <  2:91127/147‑1 (MQ=255)

                                                                                                                      gATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTa                             <  3:258396/149‑1 (MQ=255)

                                                                                                                        tCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATg                           >  2:58518/1‑149 (MQ=255)

                                                                                                                               aTCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGtata                    >  5:62079/1‑149 (MQ=255)

                                                                                                                                  cTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGtatata                  >  5:183629/1‑148 (MQ=255)

                                                                                                                                    ggAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTg                        >  3:136046/1‑140 (MQ=255)

                                                                                                                                    ggAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTg                        <  4:136046/140‑1 (MQ=255)

                                                                                                                                     gAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAAt               <  3:114147/148‑1 (MQ=255)

                                                                                                                                        ttCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAg           >  3:290073/1‑149 (MQ=255)

                                                                                                                                        accGACAGATGCACCCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAg           >  6:227027/3‑149 (MQ=255)

                                                                                                                                          cGACAGATGCACCCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCt                                                                    >  8:169269/1‑90 (MQ=255)

                                                                                                                                          cGACAGATGCACCCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCt                                                                    <  7:169269/90‑1 (MQ=255)

                                                                                                                                          cGACAGATGCACCCATATGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAgtg         >  1:305360/1‑149 (MQ=255)

                                                                                                                                           gACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAgtg         <  1:58518/148‑1 (MQ=255)

                                                                                                                                            aCAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGc                                                                                                          <  3:2345/51‑1 (MQ=255)

                                                                                                                                            aCAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGc                                                                                                          >  4:2345/1‑51 (MQ=255)

                                                                                                                                               gATGGGTTCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAGTGTCGAg    <  7:140519/149‑1 (MQ=255)

                                                                                                                                               gATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAGTGTCGAg    <  6:62079/149‑1 (MQ=255)

                                                                                                                                                 tGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTTCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAGTGTCGAGGt  <  7:227078/149‑1 (MQ=255)

                                                                                                                                                   ggTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGAGAAGCGGATACATGGCGGGCCTGCAATGAAGCGCATTGACGGAACCAAGGGCATTTTCGTATGCTTTATATAAAAAAAGTTACGAGGt  >  1:95254/1‑148 (MQ=255)

TTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAGTGTCGAGGT  >  NC_002947/2405202‑2405494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`