BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,405,350	T→C	5.3%	V116A (GTG→GCG) ‡	PP_2108 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,405,350	0	T	C	5.3%	330.4 / 5.9	112	V116A (GTG→GCG) ‡	PP_2108	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (51/55); new base C (4/2); total (55/57)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.79e-01

Only 100 of 119 total aligned reads displayed.

TTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAGTGTCGAGGTGAAG  >  NC_002947/2405202‑2405498

ttGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCTGCGGACTTCACGGTGATCGACGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGt                                                                                                                                                      <  5:248772/149‑1 (MQ=255)

ttGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGt                                                                                                                                                      >  6:277805/1‑149 (MQ=255)

ttGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGt                                                                                                                                                      <  5:253268/149‑1 (MQ=255)

ttGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGt                                                                                                                                                      <  5:69498/149‑1 (MQ=255)

gctaTCGGAGGCGCCACCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGt                                                                                                                                                      >  8:229918/4‑149 (MQ=255)

  gATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTg                                                                                                                                                     >  2:218306/1‑148 (MQ=255)

        tGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGt                                                                                                                                              <  5:259931/149‑1 (MQ=255)

        tGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGt                                                                                                                                              >  1:192815/1‑149 (MQ=255)

          gcgcCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCg                                                                                                                                            <  5:277805/149‑1 (MQ=255)

                 cGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGCACCCATCTGTCgaattcc                                                                                                                                      <  1:294365/148‑7 (MQ=255)

                      gCCAGCACGTGCAAGGGCTGGCGGCTGACTTCACGGTGATCGAGTTCAGCCCGAGCGA‑GTCCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGAGCgg                                                                                                                                 <  5:99713/148‑1 (MQ=255)

                      gCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGc                                                                                                                                <  1:200046/149‑1 (MQ=255)

                       ccAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCa                                                                                                                               >  2:88968/1‑149 (MQ=255)

                        cAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GCCCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCAc                                                                                                                              >  8:42168/1‑149 (MQ=255)

                         aGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCAcg                                                                                                                             >  5:96048/1‑149 (MQ=255)

                            aCGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCAcgccg                                                                                                                          >  3:11878/1‑149 (MQ=255)

                            aCGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCAcgccg                                                                                                                          <  4:115473/149‑1 (MQ=255)

                                gCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGGGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCgca                                                                                                                      <  4:81392/149‑1 (MQ=255)

                                gCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCcgcg                                                                                                                        >  1:242869/1‑148 (MQ=255)

                                gCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCcgcg                                                                                                                        >  4:95/1‑147 (MQ=255)

                                gCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCcgcg                                                                                                                        >  7:242756/1‑147 (MQ=255)

                                 cAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCgcag                                                                                                                     <  4:6237/149‑1 (MQ=255)

                                  aaGGGCTGTCGGCGTAATTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCgcagg                                                                                                                    <  8:257155/149‑1 (MQ=255)

                                        tGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTg                                                                                                              >  1:172647/1‑149 (MQ=255)

                                          gcggcgGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGATTCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCt                                                                                                            >  8:140519/1‑149 (MQ=255)

                                          gcggcgGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTg                                                                                                              <  2:253324/147‑1 (MQ=255)

                                               ggACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCa                                                                                                       >  3:55561/1‑149 (MQ=255)

                                                gACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCAt                                                                                                      <  2:172647/149‑1 (MQ=255)

                                                   ttCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATgcg                                                                                                   >  3:86116/1‑149 (MQ=255)

                                                   ttCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATgcg                                                                                                   <  8:242756/149‑1 (MQ=255)

                                                       cGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCaaa                                                                                               <  4:140543/149‑1 (MQ=255)

                                                       cGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCaaa                                                                                               <  3:221719/149‑1 (MQ=255)

                                                        ggTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGTTGCCCTTCGATCAGCGGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGAGCAAAg                                                                                              >  5:108891/1‑149 (MQ=255)

                                                         gTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAgg                                                                                             <  7:229918/149‑1 (MQ=255)

                                                              cGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCgcaggcag                                                                                                                 <  6:135128/124‑1 (MQ=255)

                                                               gAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGCCCATGCGCAAAGGCAGCg                                                                                        >  6:230991/1‑148 (MQ=255)

                                                               gAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCgg                                                                                       >  3:116709/1‑149 (MQ=255)

                                                                    cAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTAcc                                                                                  >  6:299706/1‑149 (MQ=255)

                                                                        ccGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGc                                                                              >  6:145274/1‑149 (MQ=255)

                                                                         cgcgcgA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGc                                                                                         <  7:60871/137‑1 (MQ=255)

                                                                         cgcgcgA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGc                                                                                         >  8:60871/1‑137 (MQ=255)

                                                                          gcgcgA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCgg                                                                            <  1:218306/149‑1 (MQ=255)

                                                                            gcgA‑GACCGTCCGGCGAATCAGCAAAAGTTCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAg                                                                                              >  7:270009/1‑129 (MQ=255)

                                                                            gcgA‑GACCGTCCGGCGAATCAGCAAAAGTTCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAg                                                                                              <  8:270009/129‑1 (MQ=255)

                                                                            gcgA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCgggg                                                                          <  4:147142/149‑1 (MQ=255)

                                                                                    cGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAAt                                                                   >  2:33763/1‑149 (MQ=255)

                                                                                        cGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAg                                                               <  5:145274/149‑1 (MQ=255)

                                                                                          gCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAgcg                                                             <  1:33763/149‑1 (MQ=255)

                                                                                          gCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAgcg                                                             >  3:28239/1‑149 (MQ=255)

                                                                                          gCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAgcg                                                             >  3:24099/1‑149 (MQ=255)

                                                                                            gAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCa                                                           >  4:258396/1‑149 (MQ=255)

                                                                                             aaTCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCAt                                                          <  2:192815/149‑1 (MQ=255)

                                                                                                cAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGt                                                                                                                                          <  1:188835/66‑1 (MQ=255)

                                                                                                cAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGt                                                                                                                                          >  2:188835/1‑66 (MQ=255)

                                                                                                cAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGa                                                       >  4:282991/1‑149 (MQ=255)

                                                                                                cAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGa                                                       >  6:163024/1‑149 (MQ=255)

                                                                                                  gCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACg                                                     >  7:181173/1‑149 (MQ=255)

                                                                                                   cAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACgg                                                    <  5:299706/149‑1 (MQ=255)

                                                                                                    aaaaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACgg                                                    >  5:253988/1‑148 (MQ=255)

                                                                                                     aaaGTGCGGTGCCCTTCGATCAGCTGATGCTGTAATTCGACAGATGGCTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGGTGACCATGCGCAAAGGGAGCGGCTACCTGGCGTGGCTGCAATGAAGCTCATTGAc                                                      <  7:84801/145‑1 (MQ=255)

                                                                                                     aaaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGgca                                                  <  5:163024/149‑1 (MQ=255)

                                                                                                     aaaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGgca                                                  <  4:116709/149‑1 (MQ=255)

                                                                                                     aaaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGAATCGGTCGGTGACGCGCGGCACGCCCCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGATGCAATGAAGCGAATTGACgag                                                   >  8:84801/1‑146 (MQ=255)

                                                                                                      aaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGgcag                                                 <  7:42168/149‑1 (MQ=255)

                                                                                                      aaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGgc                                                   <  1:316988/147‑1 (MQ=255)

                                                                                                      aaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGgc                                                   >  2:316988/1‑147 (MQ=255)

                                                                                                          gCGGAGCCCTTCGATCAGCTGAACCTGGAATTCGACAGATCGGTGCATCTGTCGGAGACGCGCGGCACGCCGCGCACGCAGGTGCTGACCATGCGCAAAGGCAGAGGCTACCTCGCGCGGCTGCAATGAAACGCATTGACGGCAGCAAg                                             >  6:270134/1‑149 (MQ=255)

                                                                                                           cGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGTAGCAAgg                                            >  6:253139/1‑149 (MQ=255)

                                                                                                           cGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAg                                             <  7:169726/148‑1 (MQ=255)

                                                                                                             gTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAgggg                                          <  4:55561/149‑1 (MQ=255)

                                                                                                                cccTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGAt                                        <  4:24099/148‑1 (MQ=255)

                                                                                                                 ccTTCGATCAGCTGATCCTGGAAATCGACAGCTGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAATCGCATTGACGGCAGCAAGGGGATGt                                      <  4:55596/149‑1 (MQ=255)

                                                                                                                   ttCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCgggg                                                                          >  8:30322/1‑111 (MQ=255)

                                                                                                                   ttCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCgggg                                                                          <  7:30322/111‑1 (MQ=255)

                                                                                                                   ttCCATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCACGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATCAAGCCCAATGACGGCAGCAAGGGGAAGTTc                                    >  8:175381/1‑149 (MQ=255)

                                                                                                                     cGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTc                                    <  2:242869/147‑1 (MQ=255)

                                                                                                                      gATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTa                                 <  3:258396/149‑1 (MQ=255)

                                                                                                                        tCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATg                               >  2:58518/1‑149 (MQ=255)

                                                                                                                           gCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTg                            >  8:144323/1‑149 (MQ=255)

                                                                                                                               aTCCTGGAATTCGACAGATGGGTGCATGTGTCGGTGACGCGGGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGtata                        <  7:175381/149‑1 (MQ=255)

                                                                                                                               aTCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGtata                        >  5:62079/1‑149 (MQ=255)

                                                                                                                                  cTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGtatata                      >  5:183629/1‑148 (MQ=255)

                                                                                                                                    ggAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTg                            >  3:136046/1‑140 (MQ=255)

                                                                                                                                    ggAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTg                            <  4:136046/140‑1 (MQ=255)

                                                                                                                                     gAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAAt                   <  3:114147/148‑1 (MQ=255)

                                                                                                                                        ttCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAg               >  3:290073/1‑149 (MQ=255)

                                                                                                                                        accGACAGATGCACCCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAg               >  6:227027/3‑149 (MQ=255)

                                                                                                                                         ccGACAGATGCACCCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAgt              >  3:104898/2‑149 (MQ=255)

                                                                                                                                          cGACAGATGCACCCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCt                                                                        <  7:169269/90‑1 (MQ=255)

                                                                                                                                          cGACAGATGCACCCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCt                                                                        >  8:169269/1‑90 (MQ=255)

                                                                                                                                          cGACAGATGCACCCATATGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAgtg             >  1:305360/1‑149 (MQ=255)

                                                                                                                                           gACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAgtgt            >  2:149796/1‑149 (MQ=255)

                                                                                                                                           gACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAgtg             <  1:58518/148‑1 (MQ=255)

                                                                                                                                            aCAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGc                                                                                                             <  3:2345/51‑1 (MQ=255)

                                                                                                                                            aCAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGc                                                                                                             >  4:2345/1‑51 (MQ=255)

                                                                                                                                               gATGGGTTCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAGTGTCGAg        <  7:140519/149‑1 (MQ=255)

                                                                                                                                               gATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAGTGTCGAg        <  6:62079/149‑1 (MQ=255)

                                                                                                                                               gATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAGTGTCGAg        <  6:183629/149‑1 (MQ=255)

                                                                                                                                                 tGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTTCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAGTGTCGAGGt      <  7:227078/149‑1 (MQ=255)

                                                                                                                                                     tGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAGTGTCGAGGTGAAg  <  8:181173/149‑1 (MQ=255)

TTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGGCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAGTGTCGAGGTGAAG  >  NC_002947/2405202‑2405498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`