BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,405,351	G→C	5.4%	V116V (GTG→GTC) ‡	PP_2108 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,405,351	0	G	C	5.4%	364.9 / 7.8	112	V116V (GTG→GTC) ‡	PP_2108	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (50/55); new base C (4/2); total (54/58)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.04e-01

Only 100 of 113 total aligned reads displayed.

GATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAGTGTCGAGGTGAAG  >  NC_002947/2405204‑2405498

      tGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGt                                                                                                                                               <  5:259931/149‑1 (MQ=255)

      tGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGt                                                                                                                                               >  1:192815/1‑149 (MQ=255)

        gcgcCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCg                                                                                                                                             <  5:277805/149‑1 (MQ=255)

               cGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGCACCCATCTGTCgaattcc                                                                                                                                       <  1:294365/148‑7 (MQ=255)

                    gCCAGCACGTGCAAGGGCTGGCGGCTGACTTCACGGTGATCGAGTTCAGCCCGAGCGA‑GTCCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGAGCgg                                                                                                                                  <  5:99713/148‑1 (MQ=255)

                    gCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGc                                                                                                                                 <  1:200046/149‑1 (MQ=255)

                     ccAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCa                                                                                                                                >  2:88968/1‑149 (MQ=255)

                      cAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GCCCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCAc                                                                                                                               >  8:42168/1‑149 (MQ=255)

                          aCGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCAcgccg                                                                                                                           >  3:11878/1‑149 (MQ=255)

                          aCGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCAcgccg                                                                                                                           <  4:115473/149‑1 (MQ=255)

                              gCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGGGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCgca                                                                                                                       <  4:81392/149‑1 (MQ=255)

                              gCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCcgcg                                                                                                                         >  1:242869/1‑148 (MQ=255)

                              gCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCcgcg                                                                                                                         >  7:242756/1‑147 (MQ=255)

                              gCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCcgcg                                                                                                                         >  4:95/1‑147 (MQ=255)

                               cAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCgcag                                                                                                                      <  4:6237/149‑1 (MQ=255)

                                aaGGGCTGTCGGCGTAATTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCgcagg                                                                                                                     <  8:257155/149‑1 (MQ=255)

                                      tGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTg                                                                                                               >  1:172647/1‑149 (MQ=255)

                                        gcggcgGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGATTCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCt                                                                                                             >  8:140519/1‑149 (MQ=255)

                                        gcggcgGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTg                                                                                                               <  2:253324/147‑1 (MQ=255)

                                        gcggcgGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCt                                                                                                             >  5:29859/1‑149 (MQ=255)

                                             ggACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCa                                                                                                        >  3:55561/1‑149 (MQ=255)

                                              gACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCAt                                                                                                       <  2:172647/149‑1 (MQ=255)

                                                 ttCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATgcg                                                                                                    >  3:86116/1‑149 (MQ=255)

                                                     cGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCaaa                                                                                                <  4:140543/149‑1 (MQ=255)

                                                     cGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCaaa                                                                                                <  3:221719/149‑1 (MQ=255)

                                                      ggTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGTTGCCCTTCGATCAGCGGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGAGCAAAg                                                                                               >  5:108891/1‑149 (MQ=255)

                                                       gTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAg‑g                                                                                             <  7:229918/149‑1 (MQ=255)

                                                          aTCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAg                                                                                          >  3:147142/1‑149 (MQ=255)

                                                            cGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCgcaggcag                                                                                                                  <  6:135128/124‑1 (MQ=255)

                                                             gAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGCCCATGCGCAAAG‑GCAGCg                                                                                        >  6:230991/1‑148 (MQ=255)

                                                             gAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCgg                                                                                       >  3:116709/1‑149 (MQ=255)

                                                                  cctatcgcgcgA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTAcc                                                                                  <  1:281989/144‑1 (MQ=255)

                                                                  cAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTAcc                                                                                  >  6:299706/1‑149 (MQ=255)

                                                                      ccGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGc                                                                              >  6:145274/1‑149 (MQ=255)

                                                                       cgcgcgA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGc                                                                                         >  8:60871/1‑137 (MQ=255)

                                                                       cgcgcgA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGc                                                                                         <  7:60871/137‑1 (MQ=255)

                                                                        gcgcgA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCgg                                                                            <  1:218306/149‑1 (MQ=255)

                                                                          gcgA‑GACCGTCCGGCGAATCAGCAAAAGTTCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAg                                                                                               >  7:270009/1‑129 (MQ=255)

                                                                          gcgA‑GACCGTCCGGCGAATCAGCAAAAGTTCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAg                                                                                               <  8:270009/129‑1 (MQ=255)

                                                                          gcgA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCgggg                                                                          <  4:147142/149‑1 (MQ=255)

                                                                                  cGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAAt                                                                   >  2:33763/1‑149 (MQ=255)

                                                                                      cGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAg                                                               <  5:145274/149‑1 (MQ=255)

                                                                                       ggCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCg                                                                             >  5:246956/1‑134 (MQ=255)

                                                                                       ggCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCg                                                                             <  6:246956/134‑1 (MQ=255)

                                                                                        gCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAgcg                                                             >  3:28239/1‑149 (MQ=255)

                                                                                        gCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAgcg                                                             <  1:33763/149‑1 (MQ=255)

                                                                                          gAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCa                                                           >  4:258396/1‑149 (MQ=255)

                                                                                           aaTCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCAt                                                          <  2:192815/149‑1 (MQ=255)

                                                                                              cAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGt                                                                                                                                           <  1:188835/66‑1 (MQ=255)

                                                                                              cAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGt                                                                                                                                           >  2:188835/1‑66 (MQ=255)

                                                                                              cAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGa                                                       >  4:282991/1‑149 (MQ=255)

                                                                                              cAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGa                                                       >  6:163024/1‑149 (MQ=255)

                                                                                                gCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACg                                                     >  7:181173/1‑149 (MQ=255)

                                                                                                gCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACg                                                     >  8:169726/1‑149 (MQ=255)

                                                                                                 cAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACgg                                                    <  5:299706/149‑1 (MQ=255)

                                                                                                  aaaaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACgg                                                    >  5:253988/1‑148 (MQ=255)

                                                                                                   aaaGTGCGGTGCCCTTCGATCAGCTGATGCTGTAATTCGACAGATGGCTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGGTGACCATGCGCAAAG‑GGAGCGGCTACCTGGCGTGGCTGCAATGAAGCTCATTGAc                                                      <  7:84801/145‑1 (MQ=255)

                                                                                                   aaaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGgca                                                  <  4:86116/149‑1 (MQ=255)

                                                                                                   aaaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGgca                                                  <  4:116709/149‑1 (MQ=255)

                                                                                                   aaaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGgca                                                  <  3:282991/149‑1 (MQ=255)

                                                                                                   aaaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGAATCGGTCGGTGACGCGCGGCACGCCCCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGATGCAATGAAGCGAATTGACgag                                                   >  8:84801/1‑146 (MQ=255)

                                                                                                    aaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGgcag                                                 <  7:42168/149‑1 (MQ=255)

                                                                                                    aaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGgc                                                   <  1:316988/147‑1 (MQ=255)

                                                                                                    aaGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGgc                                                   >  2:316988/1‑147 (MQ=255)

                                                                                                        gCGGAGCCCTTCGATCAGCTGAACCTGGAATTCGACAGATCGGTGCATCTGTCGGAGACGCGCGGCACGCCGCGCACGCAGGTGCTGACCATGCGCAAAG‑GCAGAGGCTACCTCGCGCGGCTGCAATGAAACGCATTGACGGCAGCAAg                                             >  6:270134/1‑149 (MQ=255)

                                                                                                         cGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAgg                                            <  6:96048/149‑1 (MQ=255)

                                                                                                         cGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAg                                             <  7:169726/148‑1 (MQ=255)

                                                                                                              cccTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGAt                                        <  4:28239/148‑1 (MQ=255)

                                                                                                              cccTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGAt                                        <  4:24099/148‑1 (MQ=255)

                                                                                                               ccTTCGATCAGCTGATCCTGGAAATCGACAGCTGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAATCGCATTGACGGCAGCAAGGGGATGt                                      <  4:55596/149‑1 (MQ=255)

                                                                                                                cTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGtt                                     >  8:264961/1‑149 (MQ=255)

                                                                                                                cTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGtt                                     >  7:207830/1‑149 (MQ=255)

                                                                                                                cTTCGATCAGCTGATCCTGCAATTCGTCAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGTGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGtt                                     <  2:239812/149‑1 (MQ=255)

                                                                                                                 ttCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCgggg                                                                          >  8:30322/1‑111 (MQ=255)

                                                                                                                 ttCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCgggg                                                                          <  7:30322/111‑1 (MQ=255)

                                                                                                                 ttCCATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCACGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATCAAGCCCAATGACGGCAGCAAGGGGAAGTTc                                    >  8:175381/1‑149 (MQ=255)

                                                                                                                   cGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTc                                    <  2:242869/147‑1 (MQ=255)

                                                                                                                   cGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTc                                    <  2:91127/147‑1 (MQ=255)

                                                                                                                    gATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTa                                 <  3:258396/149‑1 (MQ=255)

                                                                                                                      tCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATg                               >  2:58518/1‑149 (MQ=255)

                                                                                                                         gCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTg                            >  8:144323/1‑149 (MQ=255)

                                                                                                                             aTCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGtata                        >  5:62079/1‑149 (MQ=255)

                                                                                                                                cTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGtatata                      >  5:183629/1‑148 (MQ=255)

                                                                                                                                  ggAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTg                            >  3:136046/1‑140 (MQ=255)

                                                                                                                                  ggAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTg                            <  4:136046/140‑1 (MQ=255)

                                                                                                                                   gAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAAt                   <  3:114147/148‑1 (MQ=255)

                                                                                                                                      ttCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAg               >  3:290073/1‑149 (MQ=255)

                                                                                                                                      accGACAGATGCACCCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAg               >  6:227027/3‑149 (MQ=255)

                                                                                                                                        cGACAGATGCACCCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCt                                                                        >  8:169269/1‑90 (MQ=255)

                                                                                                                                        cGACAGATGCACCCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCt                                                                        <  7:169269/90‑1 (MQ=255)

                                                                                                                                         gACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAgtgt            >  2:149796/1‑149 (MQ=255)

                                                                                                                                         gACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAgtg             <  1:58518/148‑1 (MQ=255)

                                                                                                                                          aCAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGc                                                                                                              >  4:2345/1‑51 (MQ=255)

                                                                                                                                          aCAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGc                                                                                                              <  3:2345/51‑1 (MQ=255)

                                                                                                                                             gATGGGTTCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAGTGTCGAg        <  7:140519/149‑1 (MQ=255)

                                                                                                                                             gATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAGTGTCGAg        <  6:183629/149‑1 (MQ=255)

                                                                                                                                             gATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAGTGTCGAg        <  6:62079/149‑1 (MQ=255)

                                                                                                                                               tGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTTCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAGTGTCGAGGt      <  7:227078/149‑1 (MQ=255)

                                                                                                                                                 ggTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAGAGAAGCGGATACATGGCGGGCCTGCAATGAAGCGCATTGACGGAACCAAGGGCATTTTCGTATGCTTTATATAAAAAAAGTTACGAGGt      >  1:95254/1‑148 (MQ=255)

                                                                                                                                                   tGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAGTGTCGAGGTGAAg  <  8:181173/149‑1 (MQ=255)

GATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGA‑GACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCACGCCGCGCAGGCAGGTGCTGACCATGCGCAAAG‑GCAGCGGCTACCTGGCGGGGCTGCAATGAAGCGCATTGACGGCAGCAAGGGGATGTTCGTATGCTGTATATAAATACAGTGTCGAGGTGAAG  >  NC_002947/2405204‑2405498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`