Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,443,501	T→A	5.7%	F6Y (TTT→TAT) ‡	PP_2140 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,443,501	0	T	A	5.7%	306.8 / 5.1	98	F6Y (TTT→TAT) ‡	PP_2140	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (51/41);  new base A (4/2);  total (55/43)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

CGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAAC  >  NC_002947/2443359‑2443644                                                                                                                                                |                                                                                                                                                cGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGc                                                                                                                                           <  7:68155/149‑1 (MQ=255) cGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGc                                                                                                                                           <  5:138236/149‑1 (MQ=255)     aCAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGcccc                                                                                                                                        >  3:259877/1‑148 (MQ=255)      cAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCaa                                                                                                                                      >  8:279020/1‑149 (MQ=255)        gCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCGGATCCATTGGTGCTGTGATTTGGGGCCGCAAAGCGGCCCCaaat                                                                                                                                    <  7:174448/149‑3 (MQ=255)         ccGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCa                                                                                                                                   >  5:200426/1‑149 (MQ=255)         ccGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCa                                                                                                                                   >  2:99714/1‑149 (MQ=255)         ccGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCa                                                                                                                                   >  4:59702/1‑149 (MQ=255)           gCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGccc                                                                                                                                         <  3:26510/141‑1 (MQ=255)           gCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGccc                                                                                                                                         >  4:26510/1‑141 (MQ=255)           gCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCAtt                                                                                                                                 <  6:200426/149‑1 (MQ=255)           gCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCACTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCAtt                                                                                                                                 >  3:275344/1‑149 (MQ=255)           gCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCAGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCACTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCAtt                                                                                                                                 >  3:280009/1‑149 (MQ=255)            cAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCAAAGCGGCCCCaaatcaca                                                                                                                                <  6:333501/149‑7 (MQ=255)            cAAGACCAAGCAGCAGTATGAGCAGTCCGAGGGCGATGGAAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCAttt                                                                                                                                <  8:212702/149‑1 (MQ=255)                 ccAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTatgat                                                                                                                           <  8:282968/149‑1 (MQ=255)                  cAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTatgatg                                                                                                                          >  7:280/1‑149 (MQ=255)                    aGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCa                                                                                                                        <  2:96452/149‑1 (MQ=255)                    aGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGACTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCa                                                                                                                        <  7:282708/149‑1 (MQ=255)                           gTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTTGAAGGTTGAAGATAAGCGCTGATCCATTGGTTCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATcg                                                                                                                 <  8:251348/149‑2 (MQ=255)                           gTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATcc                                                                                                                 <  1:99714/149‑1 (MQ=255)                            tATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCa                                                                                                                                   >  5:11807/1‑130 (MQ=255)                            tATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCa                                                                                                                                   <  6:11807/130‑1 (MQ=255)                            tATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCg                                                                                                                >  3:197107/1‑149 (MQ=255)                            tATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCg                                                                                                                >  7:264544/1‑149 (MQ=255)                              tgtgCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGcc                                                                                                              >  6:194551/1‑149 (MQ=255)                               gtgCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAg                                                                                                                       >  8:266000/1‑139 (MQ=255)                               gtgCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAg                                                                                                                       <  7:266000/139‑1 (MQ=255)                                tgCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCAt                                                                                                                                  >  8:219277/1‑127 (MQ=255)                                tgCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCAt                                                                                                                                  <  7:219277/127‑1 (MQ=255)                                tgCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCtt                                                                                                            <  8:264544/149‑1 (MQ=255)                                  cAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTg                                                                                                           >  3:233438/1‑148 (MQ=255)                                     tCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGc                                                                                                       >  7:12642/1‑149 (MQ=255)                                       cGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCt                                                                                                     >  7:3754/1‑149 (MQ=255)                                        gAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCt                                                                                                     >  8:308626/1‑148 (MQ=255)                                           gTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCgg                                                                                                                                            <  2:174486/106‑1 (MQ=255)                                           gTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCgg                                                                                                                                            >  1:174486/1‑106 (MQ=255)                                           gTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGc                                                                                                               <  6:315472/135‑1 (MQ=255)                                           gTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGc                                                                                                               >  5:315472/1‑135 (MQ=255)                                              gATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGa                                                                                              <  7:279020/149‑1 (MQ=255)                                               aTGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAg                                                                                             <  4:233438/149‑1 (MQ=255)                                                tGGCAAGCCGACCGGCTGGAAGGCATGCTACGACCG‑CAATGCCGGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAgg                                                                                            <  8:173123/149‑1 (MQ=255)                                                   cAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCa                                                                                         >  2:10987/1‑149 (MQ=255)                                                    aaGCCGACCGGCTGGAAGGCATTCTACGACGGTCAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAAt                                                                                                                                     >  1:35069/1‑105 (MQ=255)                                                    aaGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAAt                                                                                                                                     <  2:35069/104‑1 (MQ=255)                                                    aaGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGcc                                                                                                              >  6:162732/1‑127 (MQ=255)                                                    aaGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGcc                                                                                                              <  5:162732/127‑1 (MQ=255)                                                    aaGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCAc                                                                                        >  8:230899/1‑149 (MQ=255)                                                      gCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACt                                                                                       >  3:212139/1‑148 (MQ=255)                                                              gCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGatat                                                                                                                   >  2:80513/1‑112 (MQ=255)                                                              gCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGatat                                                                                                                   <  1:80513/112‑1 (MQ=255)                                                                  gAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAg                                                                           <  4:138541/148‑1 (MQ=255)                                                                  gAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAg                                                                           <  7:103849/148‑1 (MQ=255)                                                                    aGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCGCAATCATTTAGGATGCAGATATGCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGaa                                                                        >  7:91025/1‑149 (MQ=255)                                                                           cTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCTCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTaca                                                                 >  7:113143/1‑149 (MQ=255)                                                                             acgacgG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACAcc                                                               >  4:182391/1‑149 (MQ=255)                                                                                            tGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGa                                                  >  2:70599/1‑148 (MQ=255)                                                                                              gAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGaaa                                                <  3:59702/148‑1 (MQ=255)                                                                                               aaGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACt                                              >  7:184781/1‑149 (MQ=255)                                                                                                  gTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGt                                            >  3:214462/1‑148 (MQ=255)                                                                                                    tGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGcc                                                                              <  7:147489/112‑1 (MQ=255)                                                                                                    tGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGcc                                                                              >  8:147489/1‑112 (MQ=255)                                                                                                    tGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGttct                                         >  1:236752/1‑149 (MQ=255)                                                                                                     gAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGttctt                                        >  8:25480/1‑149 (MQ=255)                                                                                                           aaGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCgg                                  >  8:174240/1‑149 (MQ=255)                                                                                                            aGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGc                                 >  5:270700/1‑149 (MQ=255)                                                                                                             gcgcTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCATGAACTCTACACCCGTACCAACAAGAAACTCTTCTTCGCCGGcc                                >  1:226269/1‑149 (MQ=255)                                                                                                                   aTCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGcc                           <  4:259877/148‑1 (MQ=255)                                                                                                                    tCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAACCTGTTCTTCGCCGGCCTCGCCCt                         >  7:184917/1‑149 (MQ=255)                                                                                                                     ccATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTaccaac                                                      >  1:320491/1‑119 (MQ=255)                                                                                                                     ccATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTaccaac                                                      <  2:320491/119‑1 (MQ=255)                                                                                                                      cATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTc                        <  8:184781/148‑1 (MQ=255)                                                                                                                      cATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTc                        <  2:236752/148‑1 (MQ=255)                                                                                                                       aTTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATTCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGa                      <  4:280009/149‑1 (MQ=255)                                                                                                                       aTTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGa                      <  3:182391/149‑1 (MQ=255)                                                                                                                       aTTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGa                      <  8:280/149‑1 (MQ=255)                                                                                                                       aTTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGa                      <  4:275344/149‑1 (MQ=255)                                                                                                                        ttGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTg                                             <  3:305182/125‑1 (MQ=255)                                                                                                                        ttGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTg                                             >  4:305182/1‑125 (MQ=255)                                                                                                                           gTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGaaa                                                <  4:116856/119‑1 (MQ=255)                                                                                                                           gTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGaaa                                                >  3:116856/1‑119 (MQ=255)                                                                                                                              taaatgattGGGGCCGCAAAGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATg               >  7:217304/8‑149 (MQ=255)                                                                                                                                gtgATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGc             >  8:115288/1‑149 (MQ=255)                                                                                                                                 tgATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGTTGCAGATATCCGCCTTGTTGCCTTATGGGAGGGCTCTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAATCTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGc             <  8:245509/148‑1 (MQ=255)                                                                                                                                  gATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCg                             >  7:256950/1‑131 (MQ=255)                                                                                                                                   aTTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGcc            >  8:71316/1‑147 (MQ=255)                                                                                                                                    tttGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAg         >  4:194392/1‑149 (MQ=255)                                                                                                                                    tttGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAg         <  1:70599/149‑1 (MQ=255)                                                                                                                                     ttGGGGCCGCAAAGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCACCCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGGGTCCATGGCCAAgg        >  3:48095/1‑149 (MQ=255)                                                                                                                                     ttGGGGCCGCAAAGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAgg        >  1:282510/1‑149 (MQ=255)                                                                                                                                      tGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAgg        <  7:174240/148‑1 (MQ=255)                                                                                                                                        gggCCGCAAAGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCt      >  8:254285/1‑148 (MQ=255)                                                                                                                                         ggCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGcc            <  3:47391/141‑1 (MQ=255)                                                                                                                                         ggCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGcc            >  4:47391/1‑141 (MQ=255)                                                                                                                                          gCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGaa   <  3:194392/149‑1 (MQ=255)                                                                                                                                          gCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGaa   >  3:73668/1‑149 (MQ=255)                                                                                                                                          gCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGaa   >  6:110405/1‑149 (MQ=255)                                                                                                                                            cGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAAc  >  4:126933/1‑148 (MQ=255)                                                                                                                                                |                                                                                                                                                CGCTACAGCCGCAAGACCAAGGAGCAGTATGTGCAGTCCGAGGTCGATGGCAAGCCGACCGGCTGGAAGGCATTCTACGACGG‑CAATGCCTGGAAGGTTGAAGATAAGCGCTGATCCATTGGTGCTGTGATTTGGGGCCGCTTTGCGGCCCCAATCATTTATGATGCAGATATCCGCCTTGTAGCCTTATGGGAGGGCACTGAAATGGCCCAGGAACTCTACACCCGTACCAACCAGAAACTGTTCTTCGCCGGCCTCGCCCTCGAGTCCATGGCCAAGGCTGAAC  >  NC_002947/2443359‑2443644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑