Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,628,114	T→C	5.6%	L400P (CTG→CCG)	tig →	trigger factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,628,114	0	T	C	5.6%	212.8 / 2.1	71	L400P (CTG→CCG)	tig	trigger factor
Reads supporting (aligned to +/- strand):  ref base T (35/32);  new base C (2/2);  total (37/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GGCCTGATCGTGGCCGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCG  >  NC_002947/2627972‑2628260                                                                                                                                               |                                                                                                                                                   ggCCTGATCGTGGCCGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACCGAACg                                                                                                                                               >  6:316003/1‑148 (MQ=255)    cTGATCGTGGCCGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGt                                                                                                                                           <  5:137922/149‑1 (MQ=255)    cTGATCGTGGCCGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGt                                                                                                                                           >  3:22750/1‑149 (MQ=255)            ggCCGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCgg                                                                                                                                   >  6:295153/1‑149 (MQ=255)              ccGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGtt                                                                                                                                 <  1:214280/149‑1 (MQ=255)              ccGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGtt                                                                                                                                 <  3:236454/149‑1 (MQ=255)              ccGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGtt                                                                                                                                 <  6:311396/149‑1 (MQ=255)               cGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTtg                                                                                                                                <  5:295153/149‑1 (MQ=255)               cGAAGTGGTAAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGGGAGATGATCGAAGATATGGCTTCGGCTTACCAGGAGCCTGAGCTGGTCATCTAATGGTTCTACAAGAACGAACAGCAACTGAACGAAGTGCGTTCGGTtg                                                                                                                                >  4:99726/1‑149 (MQ=255)                 aaGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTtgtg                                                                                                                              <  8:87137/149‑1 (MQ=255)                 aaGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTtgtg                                                                                                                              >  1:220805/1‑149 (MQ=255)                   gTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACCGAACGCACTTCGTTCAGTtgctgg                                                                                                                            <  5:316003/149‑5 (MQ=255)                      gTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTgg                                                                                                                          >  5:43872/1‑148 (MQ=255)                        cAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTgg                                                                                                                          >  8:214286/1‑146 (MQ=255)                         aaGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGtt                                                                                                                                      <  4:76481/133‑1 (MQ=255)                         aaGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGtt                                                                                                                                      >  3:76481/1‑133 (MQ=255)                           gCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGgaagaa                                                                                                                     >  3:108637/1‑148 (MQ=255)                           gCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAAc                                                                                                                    <  8:11702/149‑1 (MQ=255)                               ttCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGt                                                                                                                >  2:86723/1‑149 (MQ=255)                                 cGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGtt                                                                                                               <  2:118983/148‑1 (MQ=255)                                 cGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGtt                                                                                                               <  4:108637/148‑1 (MQ=255)                                  gAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGt                                                                                                             <  6:1072/149‑1 (MQ=255)                                  gAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGt                                                                                                             <  1:61618/149‑1 (MQ=255)                                    gCTGAAGCCGGACGACGCCAAGGTTCTCGAGATGATCGAAGAAATGGCTTCGGCTTACCACGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAg                                                                                                           <  4:287661/149‑1 (MQ=255)                                     cTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTTGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGa                                                                                                          <  5:232934/149‑1 (MQ=255)                                       gAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAAAGACCAGCAACTGAACGAAGTGCGTTCGGATGTGCTGGAAGAACAAGTTGTAGAt                                                                                                         >  3:244511/1‑148 (MQ=255)                                          gCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTg                                                                                                     >  4:143989/1‑149 (MQ=255)                                          gCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTg                                                                                                     >  8:47889/1‑149 (MQ=255)                                          gCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTg                                                                                                     >  6:162267/1‑149 (MQ=255)                                            cGGACGACGCCAAGGTTCGCGATATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGtt                                                                                                   <  4:22750/149‑1 (MQ=255)                                                cgacgCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGc                                                                                               <  3:143989/149‑1 (MQ=255)                                                    gCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGc                                                                                                                                         <  6:147864/103‑1 (MQ=255)                                                    gCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTgg                                                                                                                          <  8:10188/118‑1 (MQ=255)                                                    gCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTgg                                                                                                                          >  7:10188/1‑118 (MQ=255)                                                      cAAGGTTCGCGAGATGATCGAAGAAATGGCATCGGCAAACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGAGCGTTCGGTTGTGCAGGAAGAACAAGTTGAAGATACTGTTCTGCAGAAAg                                                                                         >  1:307833/1‑149 (MQ=255)                                                         ggTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGa                                                                                      >  4:315047/1‑149 (MQ=255)                                                          gTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGAc                                                                                     >  8:27864/1‑149 (MQ=255)                                                              gcgAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACtgt                                                                                  >  3:113882/1‑148 (MQ=255)                                                               cgAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGa                                                                                <  2:220805/149‑1 (MQ=255)                                                                gagaTGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGAc                                                                               <  6:43872/149‑1 (MQ=255)                                                                    tgatCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGAc                                                                           <  7:47889/149‑1 (MQ=255)                                                                       tCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAg                                                                        >  4:316059/1‑149 (MQ=255)                                                                         gaagaaATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGa                                                                                      <  3:177596/133‑1 (MQ=255)                                                                         gaagaaATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGa                                                                                      >  4:177596/1‑133 (MQ=255)                                                                          aagaaATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACCGAACGCACTTCGTTCAGTtg                                                                                                                                <  2:297977/90‑1 (MQ=38)                                                                          aagaaATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACCGAACGCACTTCGTTCAGTtg                                                                                                                                >  1:297977/1‑90 (MQ=38)                                                                             aaaTGCCATCGGCTTACCAGGAGCCTGAGCAGGCCATCGCTTGGTACTACAACAACCACCAGCAACTGAACCAAGTGCGTTCGGTTGTGCTCGAAGAACAAGTTGCAGATACTCCTCTGCAGAAAGCGACTGTGACCGACAAGTCGGtc                                                                  <  4:113882/149‑1 (MQ=255)                                                                                tGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCg                                                               <  4:225993/149‑1 (MQ=255)                                                                                               aGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGt                                                 >  1:300408/1‑148 (MQ=255)                                                                                                     cTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGAc                                                                           >  2:268489/1‑116 (MQ=255)                                                                                                     cTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCa                                          >  8:278064/1‑149 (MQ=255)                                                                                                     cTGAGCAGGTCATCGCTTGGAACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGAc                                                                           <  1:268489/116‑1 (MQ=255)                                                                                                       gAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGc                                        >  2:31859/1‑149 (MQ=255)                                                                                                         gCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCg                                      >  1:240182/1‑149 (MQ=255)                                                                                                             gTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGgc                                  <  7:214286/149‑1 (MQ=255)                                                                                                             gTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGgc                                  >  7:215392/1‑149 (MQ=255)                                                                                                              tCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGgcg                                 <  5:313424/149‑1 (MQ=255)                                                                                                               cATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTAGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGgcgc                                >  5:164096/1‑149 (MQ=255)                                                                                                                aTCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGcc                               >  2:140929/1‑149 (MQ=255)                                                                                                                 tCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCt                              >  8:35880/1‑149 (MQ=255)                                                                                                                   gCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGc                            >  4:33047/1‑149 (MQ=255)                                                                                                                          actacAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGt                                                                                                             <  7:291100/61‑1 (MQ=255)                                                                                                                          actacAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGt                                                                                                             >  8:291100/1‑61 (MQ=255)                                                                                                                           ctacAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCgctgct                     <  1:140929/148‑1 (MQ=255)                                                                                                                           ctacAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTACATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCgctgct                     <  2:300408/148‑1 (MQ=255)                                                                                                                             acAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAg                  >  1:233183/1‑149 (MQ=255)                                                                                                                                  aaCGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACg             >  1:177032/1‑149 (MQ=255)                                                                                                                                    cGACCAGCAACTGAACGACGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCGCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGt           <  6:265892/149‑1 (MQ=255)                                                                                                                                         aGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGtcttct      >  3:123922/1‑149 (MQ=255)                                                                                                                                          gCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTtctc     >  5:127202/1‑149 (MQ=255)                                                                                                                                             aCTGTACGAAGTGGGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCg  <  6:164096/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                   GGCCTGATCGTGGCCGAAGTGGTCAAGCAGTTCGAGCTGAAGCCGGACGACGCCAAGGTTCGCGAGATGATCGAAGAAATGGCTTCGGCTTACCAGGAGCCTGAGCAGGTCATCGCTTGGTACTACAAGAACGACCAGCAACTGAACGAAGTGCGTTCGGTTGTGCTGGAAGAACAAGTTGTAGATACTGTTCTGCAGAAAGCGACTGTGACCGACAAGTCGGTCTCGTACGAAGAAGCCGTTAAACCAGCCGAGGCGCCTGCCGCTGCTGAGTAACGGTCTTCTCTCG  >  NC_002947/2627972‑2628260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑