Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,669,671 T→G 6.8% intergenic (+38/+37) prpD → / ← acnB 2‑methylcitrate dehydratase/aconitate hydratase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,669,6710TG 6.8% 181.3 / 4.8 59intergenic (+38/+37)prpD/acnB2‑methylcitrate dehydratase/aconitate hydratase B
Reads supporting (aligned to +/- strand):  ref base T (31/24);  new base G (2/2);  total (33/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.50e-01

GCGACCCGCTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGAT  >  NC_002947/2669523‑2669812
                                                                                                                                                    |                                                                                                                                             
gCGACCCGCTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCAt                                                                                                                                               <  6:27481/149‑1 (MQ=255)
     ccGCTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTg                                                                                                                                          >  6:327283/1‑149 (MQ=255)
             cccGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCt                                                                                                                                  <  3:136572/149‑1 (MQ=255)
               cGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCAGCGATGCCGGGGCttt                                                                                                                                <  3:57357/149‑1 (MQ=255)
                     cGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTa                                                                                                                           >  7:38308/1‑148 (MQ=255)
                        tgtCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAAc                                                                                                                       <  5:26738/149‑1 (MQ=255)
                            agcagGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTg                                                                                                                    >  4:68590/1‑148 (MQ=255)
                                             tgCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCt                                                                                                   >  7:184876/1‑148 (MQ=255)
                                                aaGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAac                                                                                               >  5:4184/1‑149 (MQ=255)
                                                 aGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGccaagatc                                                                                              >  8:195503/1‑143 (MQ=255)
                                                        gcagAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGa                                                                                       >  6:192822/1‑149 (MQ=255)
                                                        gcagAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGa                                                                                       >  7:108534/1‑149 (MQ=255)
                                                           gAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTTGACTTGTTTGTGATATGAACCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCt                                                                                    <  1:270364/149‑1 (MQ=255)
                                                               cTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGc                                                                                >  5:44686/1‑149 (MQ=255)
                                                                tGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCg                                                                               >  7:30284/1‑149 (MQ=255)
                                                                   aaGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTg                                                                            <  4:182899/149‑1 (MQ=255)
                                                                   aaGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTg                                                                            <  3:68590/149‑1 (MQ=255)
                                                                        aCGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGc                                                                       >  4:127682/1‑149 (MQ=255)
                                                                        aCGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGc                                                                       >  6:162565/1‑149 (MQ=255)
                                                                               tgCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTcg                                                                >  6:166987/1‑149 (MQ=255)
                                                                               tgCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTc                                                                 <  8:38308/148‑1 (MQ=255)
                                                                                gCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTg                                                                            <  6:292717/136‑1 (MQ=255)
                                                                                gCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTg                                                                            >  5:292717/1‑136 (MQ=255)
                                                                                 cacaGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTcgcg                                                              >  8:74580/1‑149 (MQ=255)
                                                                                 cacaGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTcgc                                                               <  5:192822/148‑1 (MQ=255)
                                                                                   caGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCgg                                                             >  8:52471/1‑148 (MQ=255)
                                                                                    aGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGaa                                                           <  5:166987/149‑1 (MQ=255)
                                                                                          gTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAAc                                                          >  7:220522/1‑144 (MQ=255)
                                                                                          gTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAAc                                                          <  8:220522/144‑1 (MQ=255)
                                                                                          gTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAAc                                                          >  7:216223/1‑144 (MQ=255)
                                                                                          gTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGATTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAAc                                                          <  8:216223/144‑1 (MQ=255)
                                                                                              aCTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATc                                                 <  5:97115/149‑1 (MQ=255)
                                                                                              aCTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATc                                                 >  6:75919/1‑149 (MQ=255)
                                                                                                  gtttgtGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTg                                               <  7:74580/147‑1 (MQ=255)
                                                                                                   tttgtGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGt                                             <  6:4184/148‑1 (MQ=255)
                                                                                                   tttgtGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTAGCTGCCGCTTCGCGGAACTCGGCGATCTGGTc                                            <  6:44686/149‑1 (MQ=255)
                                                                                                          tatGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTc                                     <  8:108534/149‑1 (MQ=255)
                                                                                                           atGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCt                                      >  2:56410/1‑147 (MQ=255)
                                                                                                           atGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCt                                      >  7:162978/1‑147 (MQ=255)
                                                                                                           atGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGACGCTTCGCGGAACTCGGCGATCTGGTCGAAGCt                                      >  7:269238/1‑147 (MQ=255)
                                                                                                             gAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTATCTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTGGAAGCTCAgg                                  >  7:99804/1‑149 (MQ=255)
                                                                                                                  ggccggcACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCg                             <  7:52471/149‑1 (MQ=255)
                                                                                                                   gccggcACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCgg                            <  8:184876/149‑1 (MQ=255)
                                                                                                                        cacaCGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAAc                       <  2:4892/149‑1 (MQ=255)
                                                                                                                          caGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGt                     >  2:35536/1‑149 (MQ=255)
                                                                                                                           aGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTc                    <  5:327283/149‑1 (MQ=255)
                                                                                                                                 aaaCAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGc              >  5:49306/1‑149 (MQ=255)
                                                                                                                                 aaaCAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGc              >  5:62105/1‑149 (MQ=255)
                                                                                                                                     aacaaAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATg          <  3:127682/149‑1 (MQ=255)
                                                                                                                                     aacaaAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGAGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATg          >  4:294178/1‑149 (MQ=255)
                                                                                                                                      acaaAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGc         >  3:213693/1‑149 (MQ=255)
                                                                                                                                        aaaGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTg       >  6:130644/1‑149 (MQ=255)
                                                                                                                                           gCCCCGGCATCGCTGCCGGGGCTTTGGTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTc     >  2:308636/1‑148 (MQ=255)
                                                                                                                                           gCCCCGGCAGCGATGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCg                                                                     >  7:281554/1‑84 (MQ=255)
                                                                                                                                           gCCCCGGCAGCGATGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCg                                                                     <  8:281554/84‑1 (MQ=255)
                                                                                                                                            ccccGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGa   <  8:30284/149‑1 (MQ=255)
                                                                                                                                             cccGGCAGCGATGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGAt  >  2:50777/1‑149 (MQ=255)
                                                                                                                                              ccGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGAt  >  2:52611/1‑148 (MQ=255)
                                                                                                                                              ccGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGAt  <  1:52611/148‑1 (MQ=255)
                                                                                                                                               cGGAATCGCTTCCGTGGATTTTTTTACTACGTGTATCTCAGCTTACGCCTGAACCACCGGGATCTTGGCTTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCATGTAGCGGTAAACGTCGGCAGCCATGCTGTCGAt  <  5:162565/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
GCGACCCGCTTTGCCCGGCAGCGGTGTCAGCAGGTTTTCGAACTGTGCAAGGACCAGCAGAAGCTGGAAGGCACGGCTGTGCACAGGTTCGTGGACTTGTTTGTGATATGAAGCGGCCGGCACAGGCCCAAACAACAAAGCCCCGGCATCGCTGCCGGGGCTTTGTTTACAACCTGTAACTCAGCTTACGCCTGAACCACCGGGATCTTGGCGTTGGCTGCCGCTTCGCGGAACTCGGCGATCTGGTCGAAGCTCAGGTAGCGGTAAACGTCGGCAGCCATGCTGTCGAT  >  NC_002947/2669523‑2669812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑