Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,673,189 G→T 9.1% S168I (AGC→ATC)  PP_2340 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,673,1890GT 9.1% 172.6 / 7.2 55S168I (AGC→ATC) PP_2340hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (25/25);  new base T (3/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01

GAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCACTTCGCTGGTG  >  NC_002947/2673041‑2673333
                                                                                                                                                      |                                                                                                                                                
gAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAg                                                                                                                                                  >  7:119020/1‑149 (MQ=255)
        tGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCa                                                                                                                                            <  2:97563/147‑1 (MQ=255)
        tGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCa                                                                                                                                            <  1:4811/147‑1 (MQ=255)
                 gtTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGt                                                                                                                                    >  2:315350/1‑146 (MQ=255)
                 gtTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGa                                                                                                                                  <  1:315350/148‑1 (MQ=255)
                       gcCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGc                                                                                                                           >  3:193593/1‑149 (MQ=255)
                        ccGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCggg                                                                                                                            <  3:252721/147‑1 (MQ=255)
                        ccGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCggg                                                                                                                            >  4:252721/1‑147 (MQ=255)
                             gTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGaa                                                                                                                        <  4:259766/146‑1 (MQ=255)
                             gTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGaa                                                                                                                        >  3:259766/1‑146 (MQ=255)
                                   tGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGA‑GTTCTTCCTGCTTTCAGTGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGcc                                                                                                               >  6:161867/1‑149 (MQ=255)
                                       tGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGCTGCCCATGGCATCGCGCa                                                                                                                                            <  4:213371/116‑1 (MQ=255)
                                       tGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGCTGCCCATGGCATCGCGCa                                                                                                                                            >  3:213371/1‑116 (MQ=255)
                                       tGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGgtg                                                                                                           >  5:237562/1‑149 (MQ=255)
                                            aCTGCCCCAATTGCGCGATGCGATCG‑ATCGCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCTGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTc                                                                                                      <  8:119020/149‑1 (MQ=255)
                                                ccccAATTGCGCGATGCGATCG‑ATCGCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCaa                                                                                                  >  8:223334/1‑149 (MQ=255)
                                                   cAATTGCGCGATGCGATCG‑ATCGCGAGGTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAAcc                                                                                                >  1:172139/1‑149 (MQ=255)
                                                    gaactcgcgAT‑CGATCGCATCGCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGc                                                                                              >  2:24024/6‑149 (MQ=255)
                                                           cgatgcgatCG‑ATCGCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCa                                                                                       <  5:12060/149‑1 (MQ=255)
                                                            gatgcgatCG‑ATCGCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAg                                                                                      <  3:40214/149‑1 (MQ=255)
                                                                   tcg‑atcgCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGt                                                                               <  4:193593/149‑1 (MQ=255)
                                                                     g‑atcgCGA‑GTTCTTCCTGCTTTCAGAGGCACACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTg                                                                              <  7:160423/148‑1 (MQ=255)
                                                                       atcgCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGa                                                                            <  2:172139/149‑1 (MQ=255)
                                                                        tcgCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGAt                                                                           >  2:269212/1‑149 (MQ=255)
                                                                                ttcttcCTGCTTTCAGCGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAAcc                                                                    <  7:31357/149‑1 (MQ=255)
                                                                                    tcCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCg                                                                        <  3:243169/141‑1 (MQ=255)
                                                                                    tcCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCg                                                                        >  4:243169/1‑141 (MQ=255)
                                                                                     ccTGCTTTCAGTGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTgg                                                                <  5:161867/148‑1 (MQ=255)
                                                                                      cTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGa                                                               <  7:223334/148‑1 (MQ=255)
                                                                                        gCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGACACTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGTATGCGAACCTTGGACaa                                                            >  6:85634/1‑148 (MQ=255)
                                                                                          tttCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAAc                                                                     <  3:85613/138‑1 (MQ=255)
                                                                                          tttCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAAc                                                                     >  4:85613/1‑138 (MQ=255)
                                                                                            tCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTcc                                                        >  3:101893/1‑149 (MQ=255)
                                                                                                        tACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGc                                            <  4:101893/149‑1 (MQ=255)
                                                                                                            aGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGa                                        >  5:124642/1‑149 (MQ=255)
                                                                                                            aGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGa                                        >  6:44965/1‑149 (MQ=255)
                                                                                                                 tataTCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCa                                                 >  2:120102/1‑135 (MQ=255)
                                                                                                                 tataTCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCa                                                 <  1:120102/135‑1 (MQ=255)
                                                                                                                 tataTCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCt                                     >  1:48109/1‑147 (MQ=255)
                                                                                                                 tataTCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCt                                     <  2:48109/147‑1 (MQ=255)
                                                                                                                 tataTCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTgg                                   >  6:166850/1‑149 (MQ=255)
                                                                                                                  atatCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTcc                                                        <  8:5418/127‑1 (MQ=255)
                                                                                                                  atatCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTcc                                                        >  7:5418/1‑127 (MQ=255)
                                                                                                                  atatCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGt                                  >  6:282380/1‑149 (MQ=255)
                                                                                                                     tCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTcc                                <  6:124642/148‑1 (MQ=255)
                                                                                                                        cccccAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTcc                                >  3:85443/1‑145 (MQ=255)
                                                                                                                               cTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACtt                     >  7:130804/1‑149 (MQ=255)
                                                                                                                                gcccTGCGCGCTGCCCATGGCATCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGt                                  >  2:180312/3‑135 (MQ=255)
                                                                                                                                gcccTGCGCGCTGCCCATGGCATCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGt                                  <  1:180312/133‑1 (MQ=255)
                                                                                                                                gcccTGCGCGCTGCCCATGGCATCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTg                    >  4:173835/3‑149 (MQ=255)
                                                                                                                                      cgcgATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGAt               <  6:237562/148‑1 (MQ=255)
                                                                                                                                       cctctGCCAGGGGCAGCCCTCAGGGCCCGAGAGCCTGGCGAATTTGTCGTCGGGGTATTAGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGAt               <  4:18337/143‑1 (MQ=255)
                                                                                                                                            gCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCACTTCg        >  4:154162/1‑149 (MQ=255)
                                                                                                                                                 gggCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTg                                                                                            >  4:240773/1‑60 (MQ=255)
                                                                                                                                                 gggCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTg                                                                                            <  3:240773/60‑1 (MQ=255)
                                                                                                                                                  ggCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCACTTCGCtggtg  <  1:24024/149‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                
GAGGCCTATGGCATGGTGTTGCTGCCGGAGTTCCGTGACTGGGAACTGCCCCAATTGCGCGATGCGATCG‑ATCGCGA‑GTTCTTCCTGCTTTCAGAGGCGCACTACCAGCGCTATATCGCCCCCAGCTTCCTGCGCGATGCCATGGGCAGCGCGCAGGGCCAGTGAGCCGGGCGAATCAGTCGCCGGTGTATTCGCAACCGCTGGTGCAGGTTTCGTGGATGCGAACCTTGGACAGCTCCGGCATCAGCGGCTTGACCTGGTCCCAGATCCACTTGGCGATCACTTCGCTGGTG  >  NC_002947/2673041‑2673333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑