Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,694,601 T→C 100% Y298H (TAC→CAC)  PP_2362 → usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,694,6010TC100.0% 183.4 / NA 53Y298H (TAC→CAC) PP_2362usher protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/26);  total (27/26)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCTGTCACGCGACTTCGCGGCGCGTCCGGATCTTGTCACTTACCCGTTGCCTGCGTTCGCCGGCGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGTACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCACCAGCAGCCTGCTGCAGAAGGGCCTGTCGGACTACTCGGT  >  NC_002947/2694458‑2694746
                                                                                                                                               |                                                                                                                                                  
aGCTGTCACGCGACTTCGCGGCGCGTCCGGATCTTGTCACTTACCCGTTGCCTGCGTTCGCCGGCGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACAcc                                                                                                                                               >  3:275891/1‑149 (MQ=255)
     tCACGCGACTTCGCGGCGCGTCCGGATCTTGTCACTTACCCGTTGCCTGCGTTCGCCGGCGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCGAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCAc                                                                                                                                          <  4:169058/149‑1 (MQ=255)
        cgcgACTTCGCGGCGCGTCCGGATCTTGTCACTTACCCGTTGCCTGCGTTCGCCGGCGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGTCCCGCACACCCTCACCaa                                                                                                                                       <  4:275891/149‑1 (MQ=255)
                 gcggcgCGTCCGGATCTTGTCACTTACCCGTTGCCTGCGTTCGCCGGCGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGt                                                                                                                               >  7:277096/1‑148 (MQ=255)
                  cggcgCGTCCGGATCTTGTCACTTACCCGTTGCCTGCGTTCGCCGGCGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTtc                                                                                                                             <  3:44453/149‑1 (MQ=255)
                      gcgTCCGGATCTTGTCACTTACCCGTTGCCTGCGTTCGTCGGCGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTtcatc                                                                                                                          >  8:187344/1‑148 (MQ=255)
                             gATCTTGTCACTTACCCGTTGCCTGCGTTCGCCGGCGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCAAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGgtgt                                                                                                                  >  2:172035/1‑149 (MQ=255)
                                 ttGTCACTTACCCGTTGCCTGCGTTCGCCGGCGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCggg                                                                                                              >  5:335031/1‑149 (MQ=255)
                                   gTCACTTACCCGTTGCCTGCGTTCGCCGGCGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCgggg                                                                                                             <  6:335031/148‑1 (MQ=255)
                                   gTCACTTACCCGTTGCCTGCGTTCGCCGGCGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCgg                                                                                                               <  1:214486/146‑1 (MQ=255)
                                    tCACTTACCCGTTGCCTGCGTTCGCCGGCGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGa                                                                                                            <  6:119775/148‑1 (MQ=255)
                                        ttACCCGTTGCCTGCGTTCGCCGGCGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAgg                                                                                                          <  7:17731/146‑1 (MQ=255)
                                        ttACCCGTTGCCTGCGTTCGCCGGCGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAgg                                                                                                          >  8:17731/1‑146 (MQ=255)
                                            ccGTTGCCTGCGTTCGCCGGCGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCggtggt                                                                                                   >  4:101014/1‑149 (MQ=255)
                                                   cTGCGTTCGCCGGCGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTcac                                                                                             >  2:325606/1‑148 (MQ=255)
                                                   cTGCGTTCGCCGGCGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTcac                                                                                             >  2:76060/1‑148 (MQ=255)
                                                      cGTTCGTCGGCGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTcaccac                                                                                          <  7:187344/148‑1 (MQ=255)
                                                       gTTCGCCGGCGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACt                                                                                         >  5:65714/1‑148 (MQ=255)
                                                                cGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTg                                                                               <  3:101014/149‑1 (MQ=255)
                                                                 gAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTgg                                                                              >  6:212825/1‑149 (MQ=255)
                                                                   aGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGcc                                                                            >  4:256031/1‑149 (MQ=255)
                                                                    gcggcgGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCg                                                                           >  2:79463/1‑149 (MQ=255)
                                                                           tGCCGACGTCGCTCGACCTGGTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGgtg                                                                    <  7:115228/149‑1 (MQ=255)
                                                                                aCGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGac                                                              >  8:12059/1‑149 (MQ=255)
                                                                                aCGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGac                                                              <  6:65714/149‑1 (MQ=255)
                                                                                     gCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCt                                                         >  3:69967/1‑149 (MQ=255)
                                                                                          aCCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCg                                                    <  5:212825/149‑1 (MQ=255)
                                                                                               ttCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTa                                               <  1:76060/149‑1 (MQ=255)
                                                                                                  atcaACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGtatcatgc                                            >  3:72397/1‑142 (MQ=255)
                                                                                                     aaCGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCAc                                         <  1:325606/149‑1 (MQ=255)
                                                                                                      aCGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACCTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCTGCAGGTCT‑CGACCACCTTGCCGTTCTACGTCAc                                         >  1:76062/1‑148 (MQ=255)
                                                                                                        ggTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGTCCGACCACCTTGCCGTTCTACGTCACca                                       <  1:172035/149‑1 (MQ=255)
                                                                                                                 tCCAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCACCAGCAGCctgct                             <  8:277096/149‑1 (MQ=255)
                                                                                                                   cAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGa                                                               <  7:300900/113‑1 (MQ=255)
                                                                                                                   cAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGa                                                               >  8:300900/1‑113 (MQ=255)
                                                                                                                   cAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCACCAGCAGCctgctg                            >  8:255734/1‑148 (MQ=255)
                                                                                                                   cAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCACCAGCAGCCtgctgc                           <  1:79463/149‑1 (MQ=255)
                                                                                                                   cAGCACCACTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCACCAGCAGCCtgctgc                           <  2:238692/149‑1 (MQ=255)
                                                                                                                       accacTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCACCagcagc                                  >  1:273486/1‑138 (MQ=255)
                                                                                                                       accacTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCACCagcagc                                  <  2:273486/138‑1 (MQ=255)
                                                                                                                        ccacTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCACCAGCAGCCTGCTGCAGAAg                      <  7:12059/149‑1 (MQ=255)
                                                                                                                         cacTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCACCAGCAGCCTGCTGCAGAAgg                     <  7:255734/149‑1 (MQ=255)
                                                                                                                          acTGAACTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCACCAGCAGCCTGCTGCAGAAggg                    >  8:80701/1‑149 (MQ=255)
                                                                                                                              aaCTGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCACCAGCAGCCTGCTGCAGAAGGGCCt                 <  7:80701/148‑1 (MQ=255)
                                                                                                                                 tGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCACCAGCAGCctgctg                            >  8:161771/1‑134 (MQ=255)
                                                                                                                                 tGCAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCACCAGCAGCctgctg                            <  7:161771/134‑1 (MQ=255)
                                                                                                                                   cAGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCACCAGCAGCCTGCTGCAGAAGGGCCTGTCGGa           >  5:198941/1‑149 (MQ=255)
                                                                                                                                    aGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCAcc                                        <  2:95792/119‑1 (MQ=255)
                                                                                                                                    aGCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCAcc                                        >  1:95792/1‑119 (MQ=255)
                                                                                                                                     gCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCACCAGCAGCCTGCTGCAGAAGGGc                   <  7:77765/139‑1 (MQ=255)
                                                                                                                                     gCCAGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCACCAGCAGCCTGCTGCAGAAGGGc                   >  8:77765/1‑139 (MQ=255)
                                                                                                                                        aGGCCCGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCACCAGCAGCCTGCTGCAGAAGGGCCTGTCGGactact      >  2:236477/1‑149 (MQ=255)
                                                                                                                                            ccGCACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCACCAGCAGCCTGCTGCAGAAGGGCCTGTCGGACTACTCGGt  >  3:250605/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
AGCTGTCACGCGACTTCGCGGCGCGTCCGGATCTTGTCACTTACCCGTTGCCTGCGTTCGCCGGCGAAGCGGCGGTGCCGACGTCGCTCGACCTGTTCATCAACGGTTTCAAGTCCAGCACCACTGAACTGCAGCCAGGCCCGTACACCCTCACCAACGTGCCGTTCATCAACGGTGTCGGGGAGGCGGTGGTGGTCACCACTGATGCCCTTGGCCGGCAGGTGT‑CGACCACCTTGCCGTTCTACGTCACCAGCAGCCTGCTGCAGAAGGGCCTGTCGGACTACTCGGT  >  NC_002947/2694458‑2694746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑