| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,753,885 | A→G | 7.1% | T71A (ACC→GCC) ‡ | czcA‑II → | cation efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,753,885 | 0 | A | G | 7.1% | 156.3 / 2.6 | 56 | T71A (ACC→GCC) ‡ | czcA‑II | cation efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (27/25); new base G (2/2); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.71e-01 | |||||||||||
TGATGGCCGCGGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCG > NC_002947/2753739‑2754027 | tGATGGCCGCGGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGAcc > 8:289599/1‑149 (MQ=255) gcgGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGCGGTCTCGAtggc < 8:4408/149‑1 (MQ=255) acaGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAggg > 5:53494/1‑149 (MQ=255) acaGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAggg > 2:151815/1‑149 (MQ=255) ccAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCaa < 5:29858/147‑1 (MQ=255) aGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGAtggctgg > 4:267085/1‑130 (MQ=255) aGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGAtggctgg < 3:267085/130‑1 (MQ=255) aaCTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGAcc < 8:41410/149‑1 (MQ=255) tGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCcgc > 1:257576/1‑149 (MQ=255) atcgatGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCcgctcgct < 7:289599/149‑1 (MQ=255) gatGCTGTGTCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTc > 2:238528/1‑149 (MQ=255) ccGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCgg < 4:153779/148‑1 (MQ=255) cGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGc < 2:98662/149‑1 (MQ=255) tCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCg > 3:187059/1‑149 (MQ=255) cACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTg > 1:320377/1‑130 (MQ=255) cACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTg < 2:320377/130‑1 (MQ=255) cTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCaa < 2:257576/149‑1 (MQ=255) tAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGCGGTCTCGAtggcaaa > 3:186516/1‑93 (MQ=37) tAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGCGGTCTCGAtggcaaa < 4:186516/96‑4 (MQ=37) aaCGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGt > 3:29800/1‑149 (MQ=255) tCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACg < 5:79312/149‑1 (MQ=255) aGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTc > 8:289164/1‑121 (MQ=255) aGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTc < 7:289164/121‑1 (MQ=255) cAGATCAACACCGCCGCCCCCTGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCAtttt > 2:283399/1‑149 (MQ=255) cAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCAtttt < 4:187059/149‑1 (MQ=255) gATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTc > 4:23363/1‑148 (MQ=255) aTCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCga < 7:252511/149‑1 (MQ=255) aTCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCga < 5:93036/149‑1 (MQ=255) aaCACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCgatga < 6:53494/149‑1 (MQ=255) cacCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGatga‑tg < 8:276256/149‑1 (MQ=255) cacCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGatga‑tg > 4:175770/1‑149 (MQ=255) ccgccCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGGCATTTTCGATGA‑TGGCAcc > 7:254201/1‑149 (MQ=255) cccGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCgatga > 7:82324/1‑137 (MQ=255) cccGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCgatga < 8:82324/137‑1 (MQ=255) cccGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGata > 2:292879/1‑148 (MQ=255) ccGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACTCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGatat > 1:224697/1‑149 (MQ=255) ccGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATAtc > 6:118951/1‑149 (MQ=255) gccgcTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTcgccc > 6:125434/1‑149 (MQ=255) gccgcTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTcgccc > 3:235261/1‑149 (MQ=255) agcagcGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAAc < 4:29800/149‑1 (MQ=255) agcagcGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAAc > 6:170834/1‑149 (MQ=255) agcagcGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAAc < 1:283399/149‑1 (MQ=255) agcGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAg < 1:292879/149‑1 (MQ=255) gcgcATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCGAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTGCTTCGCCCGCCAACTGGTCAACGAgc < 1:151815/149‑1 (MQ=255) gcgcATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAgc < 2:122360/149‑1 (MQ=255) aTCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACACGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCt > 4:122443/1‑149 (MQ=255) tCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCAGTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCACCGAGCGCCTg > 8:280697/1‑149 (MQ=255) tCAC‑CTTTGCCATCGAGACCGCGAAGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTg > 2:312382/1‑149 (MQ=255) aC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGACTGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGc < 1:238528/149‑1 (MQ=255) tGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGt < 5:125434/147‑1 (MQ=255) tGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTg < 3:23363/148‑1 (MQ=255) gCCATCGCGGTCTCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAA‑TCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGcc > 2:71436/1‑149 (MQ=255) cATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGccc > 4:144118/1‑149 (MQ=255) aTCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCcg > 8:101422/1‑149 (MQ=255) tCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCaa > 8:224146/1‑71 (MQ=255) tCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCaa < 7:224146/71‑1 (MQ=255) | TGATGGCCGCGGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCG > NC_002947/2753739‑2754027 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |