Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,753,888 G→T 7.3% A72S (GCG→TCG)  czcA‑II → cation efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,753,8880GT 7.3% 164.5 / 4.1 55A72S (GCG→TCG) czcA‑IIcation efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (26/25);  new base T (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.21e-01

GCGGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCGCGAGCAATT  >  NC_002947/2753747‑2754036
                                                                                                                                              |                                                                                                                                                     
gcgGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGCGGTCTCGAtggc                                                                                                                                                <  8:4408/149‑1 (MQ=255)
             acaGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAggg                                                                                                                                   >  5:53494/1‑149 (MQ=255)
             acaGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAggg                                                                                                                                   >  2:151815/1‑149 (MQ=255)
                    ccAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCaa                                                                                                                              <  5:29858/147‑1 (MQ=255)
                      aGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGAtggctgg                                                                                                                                             >  4:267085/1‑130 (MQ=255)
                      aGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGAtggctgg                                                                                                                                             <  3:267085/130‑1 (MQ=255)
                         aaCTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGAcc                                                                                                                       <  8:41410/149‑1 (MQ=255)
                            tGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCcgc                                                                                                                    >  1:257576/1‑149 (MQ=255)
                                 atcgatGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCcgctcgct                                                                                                               <  7:289599/149‑1 (MQ=255)
                                    gatGCTGTGTCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTc                                                                                                            >  2:238528/1‑149 (MQ=255)
                                             ccGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCgg                                                                                                    <  4:153779/148‑1 (MQ=255)
                                              cGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGc                                                                                                  <  2:98662/149‑1 (MQ=255)
                                                    tCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCg                                                                                            >  3:187059/1‑149 (MQ=255)
                                                     cACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTg                                                                                                              >  1:320377/1‑130 (MQ=255)
                                                     cACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTg                                                                                                              <  2:320377/130‑1 (MQ=255)
                                                       cTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCaa                                                                                         <  2:257576/149‑1 (MQ=255)
                                                        tAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGCGGTCTCGAtggcaaa                                                                                                                                             <  4:186516/96‑4 (MQ=37)
                                                        tAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGCGGTCTCGAtggcaaa                                                                                                                                             >  3:186516/1‑93 (MQ=37)
                                                         aaCGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGt                                                                                       >  3:29800/1‑149 (MQ=255)
                                                             tCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACg                                                                                   <  5:79312/149‑1 (MQ=255)
                                                                aGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTc                                                                                                            <  7:289164/121‑1 (MQ=255)
                                                                aGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTc                                                                                                            >  8:289164/1‑121 (MQ=255)
                                                                     cAGATCAACACCGCCGCCCCCTGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCAtttt                                                                           >  2:283399/1‑149 (MQ=255)
                                                                     cAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCAtttt                                                                           <  4:187059/149‑1 (MQ=255)
                                                                       gATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTc                                                                          >  4:23363/1‑148 (MQ=255)
                                                                        aTCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCga                                                                        <  7:252511/149‑1 (MQ=255)
                                                                        aTCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCga                                                                        <  5:93036/149‑1 (MQ=255)
                                                                           aaCACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCgatga                                                                     <  6:53494/149‑1 (MQ=255)
                                                                             cacCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGatga‑tg                                                                  <  8:276256/149‑1 (MQ=255)
                                                                             cacCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGatga‑tg                                                                  >  4:175770/1‑149 (MQ=255)
                                                                                  ccgccCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGGCATTTTCGATGA‑TGGCAcc                                                             >  7:254201/1‑149 (MQ=255)
                                                                                       cccGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCgatga                                                                     >  7:82324/1‑137 (MQ=255)
                                                                                       cccGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCgatga                                                                     <  8:82324/137‑1 (MQ=255)
                                                                                       cccGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGata                                                         >  2:292879/1‑148 (MQ=255)
                                                                                        ccGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCACTCTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGatat                                                        >  1:224697/1‑149 (MQ=255)
                                                                                        ccGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATAtc                                                       >  6:118951/1‑149 (MQ=255)
                                                                                                  gccgcTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTcgccc                                             >  3:235261/1‑149 (MQ=255)
                                                                                                  gccgcTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTcgccc                                             >  6:125434/1‑149 (MQ=255)
                                                                                                                agcagcGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAAc                               <  4:29800/149‑1 (MQ=255)
                                                                                                                agcagcGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAAc                               <  1:283399/149‑1 (MQ=255)
                                                                                                                agcagcGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAAc                               >  6:170834/1‑149 (MQ=255)
                                                                                                                   agcGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAg                            <  1:292879/149‑1 (MQ=255)
                                                                                                                    gcgcATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCGAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTGCTTCGCCCGCCAACTGGTCAACGAgc                           <  1:151815/149‑1 (MQ=255)
                                                                                                                    gcgcATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAgc                           <  2:122360/149‑1 (MQ=255)
                                                                                                                        aTCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACACGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCt                       >  4:122443/1‑149 (MQ=255)
                                                                                                                         tCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCAGTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCACCGAGCGCCTg                      >  8:280697/1‑149 (MQ=255)
                                                                                                                         tCAC‑CTTTGCCATCGAGACCGCGAAGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTg                      >  2:312382/1‑149 (MQ=255)
                                                                                                                           aC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGACTGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGc                     <  1:238528/149‑1 (MQ=255)
                                                                                                                                 tGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGt                 <  5:125434/147‑1 (MQ=255)
                                                                                                                                 tGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTg                <  3:23363/148‑1 (MQ=255)
                                                                                                                                  gCCATCGCGGTCTCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAA‑TCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGcc             >  2:71436/1‑149 (MQ=255)
                                                                                                                                    cATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGccc            >  4:144118/1‑149 (MQ=255)
                                                                                                                                     aTCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCcg           >  8:101422/1‑149 (MQ=255)
                                                                                                                                      tCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCaa                                                                                         <  7:224146/71‑1 (MQ=255)
                                                                                                                                      tCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCaa                                                                                         >  8:224146/1‑71 (MQ=255)
                                                                                                                                              gcgATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCGCGAGCAAtt  <  3:175770/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                     
GCGGTGGGTATCCACAGCTACCAGAAACTGCCGATCGATGCTGTGCCGGACATCACTAACGTCCAGGTGCAGATCAACACCGCCGCCCCCGGCTATTCGCCGCTGGAGACCGAGCAGCGCATCAC‑CTTTGCCATCGAGACCGCGATGGCTGGCCTGCCAGGGCTCAAGCAGACCCGCTCGCTGTCGCGCTCGGGCCTGTCGCAAGTCACGGTCATTTTCGATGA‑TGGCACCGATATCTTCTTCGCCCGCCAACTGGTCAACGAGCGCCTGCAGGTGGCCCGCGAGCAATT  >  NC_002947/2753747‑2754036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑