Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,787,623 G→A 9.6% intergenic (+70/‑55) ahpC → / → ahpF peroxiredoxin/alkylhydroperoxide reductase small subunit/alkyl hydroperoxide reductase subunit F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,787,6230GA 9.6% 135.9 / 5.2 52intergenic (+70/‑55)ahpC/ahpFperoxiredoxin/alkylhydroperoxide reductase small subunit/alkyl hydroperoxide reductase subunit F
Reads supporting (aligned to +/- strand):  ref base G (25/22);  new base A (2/3);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.98e-01

AGGCGAAGTGTGCCCGGCCAAGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCG  >  NC_002947/2787475‑2787768
                                                                                                                                                    |                                                                                                                                                 
aGGCGAAGTGTGCCCGGCCAAGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCg                                                                                                                                                   <  1:48277/149‑1 (MQ=255)
      aGTGTGCCCGGCCAAGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGAc                                                                                                                                             <  4:18465/149‑1 (MQ=255)
      aGTGTGCCCGGCCAAGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGAc                                                                                                                                             <  4:89038/149‑1 (MQ=255)
                gCCAAGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTggg                                                                                                                                   >  4:216607/1‑149 (MQ=255)
                   aaGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCAGGCGAGGTCGCCCGGGCGt                                                                                                                                <  7:307527/149‑1 (MQ=255)
                    aGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGtt                                                                                                                               >  3:271430/1‑149 (MQ=255)
                        gAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGttttt                                                                                                                            <  2:67781/148‑1 (MQ=255)
                        gAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCAGGCGAGGTCGCCCGGGCGtt                                                                                                                               >  7:102799/1‑145 (MQ=255)
                        gAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCAGGCGAGGTCGCCCGGGCGtt                                                                                                                               <  8:102799/145‑1 (MQ=255)
                           agaagGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTAt                                                                                                                        <  2:48551/149‑1 (MQ=255)
                           agaagGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTAt                                                                                                                        >  7:200205/1‑149 (MQ=255)
                             aagGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATcc                                                                                                                      >  3:255395/1‑149 (MQ=255)
                                  gAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCgg                                                                                                                                                  >  3:242333/1‑116 (MQ=255)
                                  gAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCgg                                                                                                                                                  <  4:242333/116‑1 (MQ=255)
                                  gAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGtt                                                                                                                 >  7:39363/1‑149 (MQ=255)
                                            tGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGaaaaa                                                                                                         <  4:255395/147‑1 (MQ=255)
                                             ggCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAgg                                                                                                       >  2:1720/1‑148 (MQ=255)
                                                  cGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGc                                                                                                 <  3:216607/149‑1 (MQ=255)
                                                                gTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGAc                                                                                    <  7:170739/148‑1 (MQ=255)
                                                                    gCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCAc                                                                               >  2:50633/1‑149 (MQ=255)
                                                                     cAAGATCTAAGGTCATGCCAATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACg                                                                              >  8:188763/1‑149 (MQ=255)
                                                                            tAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAAt                                                                       >  6:197127/1‑149 (MQ=255)
                                                                              aGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGTCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATc                                                                      <  6:190193/148‑1 (MQ=255)
                                                                                        cATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGaaa                                                            >  8:229792/1‑148 (MQ=255)
                                                                                                  gcggAAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGa                                                 >  6:238723/1‑149 (MQ=255)
                                                                                                    ggTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGc                                               <  8:200205/149‑1 (MQ=255)
                                                                                                      tAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCgg                                             <  5:197127/149‑1 (MQ=255)
                                                                                                      tAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCgg                                             <  6:188302/149‑1 (MQ=255)
                                                                                                      tAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGAGACCTCGCCTGGGCGTTTTTGTATACGAGTTTTGAAAAAGGAATCGACCGTATGTTGGACGCCACGCGTAAATCTAAACTGAAAAACTACTTGGAgatg                                             >  5:38148/1‑146 (MQ=255)
                                                                                                        agcagcCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGACCGGGCGACCTCGCCTGGGCGTTTTTGTATCCTAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGt                                           <  7:188763/149‑1 (MQ=255)
                                                                                                         gcagcCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGt                                           >  4:136127/1‑148 (MQ=255)
                                                                                                         gcagcCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTc                                          <  7:229792/149‑1 (MQ=255)
                                                                                                          cagcCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGaaaa                                                           <  5:114058/131‑1 (MQ=255)
                                                                                                          cagcCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGaaaa                                                           >  6:114058/1‑131 (MQ=255)
                                                                                                          cagcCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCa                                         >  2:178822/1‑149 (MQ=255)
                                                                                                             ccgccAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGATGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCAccc                                      >  2:29206/1‑149 (MQ=255)
                                                                                                               gccAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAg                                    <  8:39363/149‑1 (MQ=255)
                                                                                                                  aaaGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGt                                                                                                                          >  1:323940/1‑60 (MQ=255)
                                                                                                                  aaaGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGt                                                                                                                          <  2:323940/60‑1 (MQ=255)
                                                                                                                  aaaGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGcc                                  >  5:183460/1‑148 (MQ=255)
                                                                                                                     gCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGACACCCAGCCGATc                              <  5:238723/149‑1 (MQ=255)
                                                                                                                       tGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATc                              >  6:182306/1‑147 (MQ=255)
                                                                                                                       tGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATc                              >  4:177555/1‑147 (MQ=255)
                                                                                                                              cctaccGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACTCAGCCGATCGAGATCGtt                     <  4:271430/149‑1 (MQ=255)
                                                                                                                              cctaccGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGtt                     <  1:1720/149‑1 (MQ=255)
                                                                                                                              cctaccGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGtt                     >  6:134030/1‑149 (MQ=255)
                                                                                                                              cctaccGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGtt                     <  5:182306/149‑1 (MQ=255)
                                                                                                                                 accGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTCGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTgg                                                  >  1:122660/1‑117 (MQ=255)
                                                                                                                                 accGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTCGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTgg                                                  <  2:122660/117‑1 (MQ=255)
                                                                                                                                  cctcaccGTCAAACGCCCGGGCGACCTCGCCTGGTCGTTTTTGTATCCTAGTTTTTAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCtt                 <  6:38148/144‑1 (MQ=255)
                                                                                                                                       tacaaaaaCGCCCAGGCGAGGTCGCCCGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCtt                 <  5:208619/139‑1 (MQ=39)
                                                                                                                                       tacaaaaaCGCCCAGGCGAGGTCGCCCGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCtt                 >  6:208619/6‑144 (MQ=39)
                                                                                                                                             aaCGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCgacgac      >  6:97549/1‑149 (MQ=255)
                                                                                                                                              aCGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGacgacg     >  6:213618/1‑149 (MQ=255)
                                                                                                                                                 cccGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGgcg  >  6:238382/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AGGCGAAGTGTGCCCGGCCAAGTGGAAAGAAGGCGAAGCCACTCTGGCGCCGTCGCTGGACCTCGTAGGCAAGATCTAAGGTCATGCCCATAACGCGGGCGGTAAGCAGCCGCCAAAGCTGAAGTACCTACCGCCCCGTCAAACGCCCGGGCGACCTCGCCTGGGCGTTTTTGTATCCGAGTTTTGAAAAAGGAATCGCCCGTATGTTGGACGCCACGCTTAAATCGCAACTGAAAACCTACTTGGAGCGGGTCACCCAGCCGATCGAGATCGTTGCTTCCCTCGACGACGGCG  >  NC_002947/2787475‑2787768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑