Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,789,594	G→C	6.1%	*63S (TGA→TCA)	PP_2441 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,789,594	0	G	C	6.1%	207.0 / 3.0	65	*63S (TGA→TCA)	PP_2441	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (31/30);  new base C (2/2);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

CAAAGCCCGGGCAGCAAGAGCAGGACCTGCACTGGGGCTACCTGGAAATCTACGCCGACGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGCGCCGCCTTCCTGCTGCCGTGCAGCGAGAATCGCCTCGATTTCC  >  NC_002947/2789447‑2789741                                                                                                                                                    |                                                                                                                                                     cAAAGCCCGGGCAGCAAGAGCAGGACCTGCACTGGGGCTACCTGGAAATCTACGCCGACGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGa                                                                                                                                                     <  4:92254/149‑1 (MQ=255)        cGGGCAGCAAGAGCAGGACCTGCACTGGGGCTACCTGGAAATCTACGCCGACGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCa                                                                                                                                              <  1:33274/149‑1 (MQ=255)              gcaAGAGCAGGACCTGCACTGGGGCTACCTGGAAATCTACGCCGACGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTc                                                                                                                                        >  3:150191/1‑149 (MQ=255)                aaGAGCAGGACCTGCACTGGGGCTACCTGGAAATCTACGCCGACGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCa                                                                                                                                      <  4:188737/149‑1 (MQ=255)                  gagCAGGACCTGCACTGGGGCTACCTGGAAATCTACGCCGACGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGc                                                                                                                                    >  8:1721/1‑149 (MQ=255)                           cTGCACTGGGGCTACCTGGAAATCTACGCCGACGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCTGAGCCGTCACGGCTcggggtc                                                                                                                                         <  6:70443/135‑7 (MQ=255)                           cTGCACTGGGGCTACCTGGAAATCTACGCCGACGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCTGAGCCGTCACGGCTcggggtc                                                                                                                                         >  5:70443/1‑129 (MQ=255)                               aCTGGGGCTACCTGGAAATCTACGCCGACGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGgcgcg                                                                                                                      >  3:77973/1‑149 (MQ=255)                                   gggCTACCTGGAAATCTACGCCGACGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTc                                                                                                                  >  4:49649/1‑149 (MQ=255)                                   gggCTACCTGGAAATCTACGCCGACGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTc                                                                                                                  >  5:331988/1‑149 (MQ=255)                                    ggCTACCTGGAAATCTACGCCGACGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTc                                                                                                                  >  2:97074/1‑148 (MQ=255)                                     gCTACCTGGAAATCTACGCCGACGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCa                                                                                                                <  8:131614/149‑1 (MQ=255)                                         ccTGGAAATCTACGCCGACGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAg                                                                                                                                     <  4:84709/125‑1 (MQ=255)                                         ccTGGAAATCTACGCCGACGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAg                                                                                                                                     >  3:84709/1‑125 (MQ=255)                                            ggAAATCTACGCCGACGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGcgccg                                                                                                          >  2:298085/1‑148 (MQ=255)                                              aaaTCTACGCCGACGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCaa                                                                                                       >  5:315251/1‑149 (MQ=255)                                                     cGCCGACGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCgggc                                                                                                <  3:11326/149‑1 (MQ=255)                                                      gCCGACGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCGGCAATTTCCCCGACACCGAGCCGTGACGGCTCAGACTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGAAATGCgggct                                                                                               >  6:326855/1‑149 (MQ=255)                                                           cGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCgggcttcgag                                                                                          <  4:281005/149‑1 (MQ=255)                                                            ggTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCgggcttcgagg                                                                                         <  4:150191/149‑1 (MQ=255)                                                                 cgcTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCgggcttcgagggcttc                                                                                    >  5:178908/1‑149 (MQ=255)                                                                  gcTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCgggcttcgagggcttcg                                                                                   <  1:320571/149‑1 (MQ=255)                                                                      tgtTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCTCCGCAATGCGGGCTTCGAGGGCTTCGATg                                                                                <  4:58638/148‑1 (MQ=255)                                                                      tgtTCCTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGAt                                                                                 <  4:210477/147‑1 (MQ=255)                                                                              gACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGtcttct                                                                       <  2:217492/149‑1 (MQ=255)                                                                              gACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGtcttct                                                                       <  7:89004/149‑1 (MQ=255)                                                                                    cgcCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGacaca                                                                 >  2:107748/1‑149 (MQ=255)                                                                                        cTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTgc                                                             >  6:311412/1‑149 (MQ=255)                                                                                        cTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTgc                                                             <  6:178908/149‑1 (MQ=255)                                                                                              aCCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCAc                                                       <  3:49649/149‑1 (MQ=255)                                                                                              aCCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCAc                                                       >  3:101254/1‑149 (MQ=255)                                                                                               ccGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTCACgg                                                                                                                                                  <  1:288935/58‑1 (MQ=255)                                                                                               ccGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTCACgg                                                                                                                                                  >  2:288935/1‑58 (MQ=255)                                                                                                cGGGAGGTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGAGCCCGAGCCGTGTCGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTg                                                     <  8:64481/149‑1 (MQ=255)                                                                                                  ggAGCTGGCCGAGCGCAAGAGCTGCGGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCATGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGt                                                   <  4:77973/149‑1 (MQ=255)                                                                                                  ggAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGt                                                   <  6:315251/149‑1 (MQ=255)                                                                                                      cTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGt                                                   <  7:248174/145‑1 (MQ=255)                                                                                                      cTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGt                                                   >  8:248174/1‑145 (MQ=255)                                                                                                      cTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTgcgc                                               <  1:107748/149‑1 (MQ=255)                                                                                                         gCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGATGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTgcgcgcg                                            >  4:259663/1‑149 (MQ=255)                                                                                                           cGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGtcttct                                                                       <  7:240659/120‑1 (MQ=255)                                                                                                           cGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGtcttct                                                                       >  8:240659/1‑120 (MQ=255)                                                                                                           cGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGcgcc                                          <  1:97074/149‑1 (MQ=255)                                                                                                           cGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGcgcc                                          >  1:139954/1‑149 (MQ=255)                                                                                                            gAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCCCTGGTGCGCGcgccg                                         >  5:48240/1‑149 (MQ=255)                                                                                                              gcgcAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGCgccgcc                                       >  2:66491/1‑149 (MQ=255)                                                                                                                gcAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGCGCCGCCtt                                     >  8:254806/1‑149 (MQ=255)                                                                                                                gcAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGCGCCGCAtt                                     >  6:58317/1‑149 (MQ=255)                                                                                                                 cAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGCGCCGCCTTc                                    <  6:331988/149‑1 (MQ=255)                                                                                                                     agCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGCGCCGCCTTCctgc                                >  7:240121/1‑149 (MQ=255)                                                                                                                          ccGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGCGCCGCCTTCCTGCTGCCg                           >  3:136876/1‑149 (MQ=255)                                                                                                                          ccGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGCGCCGCCTTCCTGCTGCCg                           >  5:209650/1‑149 (MQ=255)                                                                                                                              aaTTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGCGCCGCCTTCCTGCTGCCGTGCa                       <  6:209650/149‑1 (MQ=255)                                                                                                                               aTTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGCGCCGCCTTCCTGCTGCCGTGCAg                      >  5:125850/1‑149 (MQ=255)                                                                                                                                 ttCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGCGCCGCCTTCCTGCTGCCGTGCAGc                     >  1:163845/1‑148 (MQ=255)                                                                                                                                     ccgaccccgaGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATg                                                                                >  6:102479/1‑85 (MQ=255)                                                                                                                                     ccgaccccgaGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATg                                                                                <  5:102479/85‑1 (MQ=255)                                                                                                                                      cgaccccgaGCCGTGACGGCTCAGCCTTCCAGCTCATGCCCGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGCGCCGCCTTCCTGCTGCCGTGCAGCGAGaa                >  1:220823/1‑149 (MQ=255)                                                                                                                                        accccgaGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGCGCCGCCTTCCTGCTGCCGTGCAGCGAGAATCg             <  6:125850/149‑1 (MQ=255)                                                                                                                                         ccccgaGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGCGCCGCCTTCCTGCTGCCGTGCAGCgag                  <  4:273440/143‑1 (MQ=255)                                                                                                                                         ccccgaGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGCGCCGCCTTCCTGCTGCCGTGCAGCgag                  >  3:273440/1‑143 (MQ=255)                                                                                                                                          cccgaGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGCGCCGCCTTCCTGCTGCCGTGCAGCGAGAATCGcc           <  1:298085/149‑1 (MQ=255)                                                                                                                                          cccgaGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGCGCCGCCTTCCTGCTGCCGTGCAGCGAGAATCGcc           >  6:270104/1‑149 (MQ=255)                                                                                                                                                ccGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGCGCCGCCTTCCTGCTGCCGTGCAGCGAGAATCGCCTCGAtt     <  4:136876/149‑1 (MQ=255)                                                                                                                                                   tGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGCGCCGCCTTCCTGCTGCCGTGCAGCGAGAATCGCCTCGATTTcc  <  2:139954/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CAAAGCCCGGGCAGCAAGAGCAGGACCTGCACTGGGGCTACCTGGAAATCTACGCCGACGGTCGCGCTGTGTTCGTCGACCAGCGCCCTACTGACCGGGAGCTGGCCGAGCGCAAGAGCTGCCGCAATTTCCCCGACCCCGAGCCGTGACGGCTCAGCCTTCCAGCTCA‑GCCAGGCGCGCTTCCAGCGCCGCAATGCGGGCTTCGAGGGCTTCGATGCGGTCTTCTGACACACTGCCGCCACTGGTGCGCGCGCCGCCTTCCTGCTGCCGTGCAGCGAGAATCGCCTCGATTTCC  >  NC_002947/2789447‑2789741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑