| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,822,277 | G→A | 7.7% | A186T (GCC→ACC) | PP_2475 → | TetR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,822,277 | 0 | G | A | 7.7% | 148.1 / 2.2 | 52 | A186T (GCC→ACC) | PP_2475 | TetR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (23/25); new base A (2/2); total (25/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.84e-01 | |||||||||||
TGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACTGACCAACTAGTCGACTGGTCTAATTCGGGTGATCGAGACAAGCCCAACTTCAGCAACCCCAGGAGTTTG > NC_002947/2822132‑2822424 | tGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCca > 7:237528/1‑149 (MQ=255) gAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGATGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCca > 5:8536/1‑148 (MQ=255) tGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAAc > 8:26209/1‑149 (MQ=255) ttGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCa < 5:91569/148‑1 (MQ=255) gAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGTCACAACTCAGa < 8:48710/148‑1 (MQ=255) gAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTc < 4:127185/145‑1 (MQ=255) gAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTc > 3:127185/1‑145 (MQ=255) aGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCATTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGAGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGAc > 1:205231/1‑148 (MQ=255) aGGTGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCGACTCTGCGACTGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCAtt < 6:8536/149‑1 (MQ=255) tctGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGTTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCt > 2:98720/1‑149 (MQ=255) aaTGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑gc > 3:178253/1‑149 (MQ=255) aTGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGc > 5:217017/1‑149 (MQ=255) tGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGc < 6:217017/148‑1 (MQ=255) cTTTCCACACTGCGAGGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTTCTGAGCAAGCTTCACCGTAATAGTCGGGCGCTGGAAATCGGCATGGCCACAACTGAGACGATTCTGTCCTCTGACAACATATGATG‑GCGCGGTTGCCCa < 2:205231/149‑1 (MQ=255) cTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCa > 4:141487/1‑149 (MQ=255) tttCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCAAAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCAc > 8:32142/1‑149 (MQ=255) tttCCACACTCCGACGGCCCTTTACCAGTTGTGGCACGGTTCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCAc < 5:43126/149‑1 (MQ=255) acacTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGtt > 3:12628/1‑149 (MQ=255) cacTGCGACGGCCCTTTACCAGTTGTGGATCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTtg < 4:4453/149‑1 (MQ=255) tGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGcc > 6:21468/1‑88 (MQ=255) tGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGcc < 5:21468/88‑1 (MQ=255) cGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTtggtgg < 3:141487/148‑1 (MQ=255) cGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTtggtgg < 8:237528/148‑1 (MQ=255) tttACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCAtttttt > 2:41500/1‑148 (MQ=255) tACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTc > 7:31534/1‑148 (MQ=255) aCCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCa < 7:32142/148‑1 (MQ=255) aCCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGG‑AAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCa < 5:300577/147‑1 (MQ=255) aCCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGG‑AAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCa > 6:300577/1‑147 (MQ=255) cAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTtggt > 4:319396/1‑132 (MQ=255) cAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTtggt < 3:319396/132‑1 (MQ=255) cAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACt > 6:251963/1‑148 (MQ=255) aGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACTGa < 5:251963/149‑1 (MQ=255) gTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTtg > 4:207840/1‑128 (MQ=255) gTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTtg < 3:207840/128‑1 (MQ=255) tgGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACTGACCaa < 4:178253/148‑1 (MQ=255) tCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACTGACCAACTAGt > 4:302826/1‑149 (MQ=255) tCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATATTTTTCACTGACCAagtagt > 4:298095/1‑149 (MQ=255) ggTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCGACCTGTTGGTGGGCATTTTTTTCACTGACCAACTAGt < 3:298095/147‑1 (MQ=255) ggTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACTGACCAACTAGt < 3:302826/147‑1 (MQ=255) tGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGACCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACTGACCAACTAGTCGAc > 2:100570/1‑149 (MQ=255) gAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACTGACCAACt > 3:309657/1‑130 (MQ=255) gAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACTGACCAACt < 4:309657/130‑1 (MQ=255) gCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATGTACGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACTGACCAACTAGTCGACTGGTCTAATTCgg < 1:98720/149‑1 (MQ=255) aGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACTGACCAACTAGTCGACTGGTCTAATTCGGGTGa > 8:198046/1‑149 (MQ=255) cacCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACTGACCAACTAGTCGACTGGTCTAATTCGGGTGATCga < 3:122636/148‑1 (MQ=255) cGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACTGACCAACTAGTCGACTGGTCTAATTCGGGTGATCGAGAc > 2:24209/1‑148 (MQ=255) gTAATAGTCGGCCGCTGGAAAACGCCATGACCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACTGACCAACTAGTCGACTGGTCTAATTCGGGTGATCGAGACaa < 1:100570/149‑1 (MQ=255) tCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACTGACCAACTAGTCGACTGGTCTAATTCGGGTGATCGAGACAAGCCCAAc < 1:24209/149‑1 (MQ=255) ggCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACTGACCAACTAGTCGACTGGTCTAATTCGGGTGATCGAGACAAGCCCAACt < 7:198046/148‑1 (MQ=255) gAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACTGACCAACTAGTCGACTGGTCTAATTCGGGTGATCGAGACAAGCCCAACTTCAGCAAc < 7:26209/148‑1 (MQ=255) ccATGACCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACTGACCAACTAGTCGACTGGTCTAATTCGGGTGATCGAGACAAGCCCAAc < 4:13641/132‑1 (MQ=255) ccATGACCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACTGACCAACTAGTCGACTGGTCTAATTCGGGTGATCGAGACAAGCCCAAc > 3:13641/1‑132 (MQ=255) gCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACTGACCAACTAGTCGACTGGTCTATTTCGGGTGAGCGAGACAAGCCCAACTTCAGCAACCCCAGGAGTTTg > 8:282410/1‑148 (MQ=255) | TGAATGTATTGAGCAGGGGCAGCGAGACGGTTCTCTGGCAATGGGCGATGCTTTCCACACTGCGACGGCCCTTTACCAGTTGTGGCTCGGTGCGAGTCTGCTGAGCAAGCTACACCGTAATAGTCGGCCGCTGGAAAACGCCATGGCCACAACTCAGACCATTCTGACCTCTGACAACATCTGATG‑GCGCGGTTGCCCACCTGTTGGTGGGCATTTTTTTCACTGACCAACTAGTCGACTGGTCTAATTCGGGTGATCGAGACAAGCCCAACTTCAGCAACCCCAGGAGTTTG > NC_002947/2822132‑2822424 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |