Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,826,935	T→G	6.8%	D116E (GAT→GAG)	PP_2479 →	cytochrome c family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,826,935	0	T	G	6.8%	165.1 / 2.6	55	D116E (GAT→GAG)	PP_2479	cytochrome c family protein
Reads supporting (aligned to +/- strand):  ref base T (27/24);  new base G (2/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

CCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAATTTGCTGTTCCTTAACGATGAGCCATTTCAGGC  >  NC_002947/2826789‑2827081                                                                                                                                                    |                                                                                                                                                   cccAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGa                                                                                                                                                  >  2:98762/1‑149 (MQ=255) cccAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGa                                                                                                                                                  >  2:182816/1‑149 (MQ=255)  ccAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGa                                                                                                                                                  >  6:99163/1‑148 (MQ=255)  ccAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGa                                                                                                                                                  >  1:303865/1‑148 (MQ=255)  ccAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGAc                                                                                                                                                 <  4:66588/149‑1 (MQ=255)          aCCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTcg                                                                                                                                          <  1:182816/148‑1 (MQ=255)             ccGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGAt                                                                                                                                      >  2:42035/1‑149 (MQ=255)                 aCCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAg                                                                                                                                  <  6:72571/149‑1 (MQ=255)                  cccGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAgg                                                                                                                                 <  1:42035/149‑1 (MQ=255)                     gCAAACCTGCATCGGTTAATGGACCGATGACGAGTTCGTCAGTGAGCAGC‑AGAACTGCATTGGCATTGAAGGCAAGCACTACTACCCGTAGTTTACATATACATCATACAGCAAAATGTCGAGCGATGAAATCATCGCGATCAAGGGt                                                                                                                               >  8:73279/1‑148 (MQ=255)                     gCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGt                                                                                                                               >  2:85119/1‑148 (MQ=255)                        aaCCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTa                                                                                                                             <  7:298788/147‑1 (MQ=255)                        aaCCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTa                                                                                                                             >  8:298788/1‑147 (MQ=255)                         aCCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTAtct                                                                                                                          <  1:202439/149‑1 (MQ=255)                          tcgtcATCGGTCCATGCACCGATGACGAGTTCGTCAGTGCGCTGCGAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTAtct                                                                                                                          >  1:234351/5‑149 (MQ=255)                            ggCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGa                                                                                                                       <  2:99544/149‑1 (MQ=255)                             gCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGa                                                                                                                       >  7:246353/1‑148 (MQ=255)                               aTCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAg                                                                                                                    >  5:23811/1‑149 (MQ=255)                                            ccGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGta                                                                                                       <  1:85119/149‑2 (MQ=255)                                             cGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTc                                                                                                       >  6:220885/1‑148 (MQ=255)                                              gATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGa                                                                                                     <  6:271606/149‑1 (MQ=255)                                              gATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGa                                                                                                     <  8:246353/149‑1 (MQ=255)                                                tGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGag                                                                                                    >  2:172126/1‑148 (MQ=255)                                                    gAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCg                                                                                                                        <  1:176424/124‑1 (MQ=255)                                                    gAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCg                                                                                                                        >  2:176424/1‑124 (MQ=255)                                                                    tGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACca                                                                                >  5:210613/1‑148 (MQ=255)                                                                            cGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCt                                                                        >  8:200841/1‑149 (MQ=255)                                                                              gCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTc                                                                      >  4:154783/1‑149 (MQ=255)                                                                               cATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTcc                                                                     <  2:242930/149‑1 (MQ=255)                                                                                aTTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTccc                                                                    <  1:172126/149‑1 (MQ=255)                                                                                       gTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGa                                                                                                     <  4:206058/109‑1 (MQ=255)                                                                                       gTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGa                                                                                                     >  3:206058/1‑109 (MQ=255)                                                                                          aCGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCa                                                           <  5:220885/148‑1 (MQ=255)                                                                                                       ctacCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATg                                              <  2:303865/148‑1 (MQ=255)                                                                                                         acCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACcgcgc                                                                                        >  4:43551/1‑104 (MQ=255)                                                                                                         acCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACcgcgc                                                                                        <  3:43551/104‑1 (MQ=255)                                                                                                           ccGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGtttt                                         >  1:318016/1‑149 (MQ=255)                                                                                                           ccGGCGTTTCCCTATACCTCCAACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTGATCTCGACAGCCTGCGGCCGGTCGTGCAAGCACCGCGCGAAGACCACATCGGCGTCCCGTTCAATCAGCGCAGGGGCATGGttgt                                         >  8:205482/1‑147 (MQ=255)                                                                                                            cGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTc                                        <  6:23811/149‑1 (MQ=255)                                                                                                                    cccTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATAAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAAttt                                >  1:70946/1‑149 (MQ=255)                                                                                                                     ccTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGGTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAATTTg                               >  1:251705/1‑149 (MQ=255)                                                                                                                      cTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTccc                                                                    >  7:92746/1‑111 (MQ=255)                                                                                                                      cTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTccc                                                                    <  8:92746/111‑1 (MQ=255)                                                                                                                           cctcctACAGCAAAATGTCGCGCTATGACATCCTCGCGATCAATGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAATTTGCTGTTc                         <  7:73279/149‑1 (MQ=255)                                                                                                                           cctcctACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAATTTGCTGtt                          <  2:251705/148‑1 (MQ=255)                                                                                                                                    gCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTATGGAATTTGCTGTTCCTTAACGAt                <  7:200841/149‑1 (MQ=255)                                                                                                                                     cAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCT‑GAATTTGCTGTTCCTTAACGAt                >  3:93976/1‑147 (MQ=255)                                                                                                                                       aaaTGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAATTTGCTGTTCCTTAACGATGAg             >  6:145978/1‑149 (MQ=255)                                                                                                                                              tcgcgAGGATGTCATCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGc                                                                         >  4:217747/2‑82 (MQ=255)                                                                                                                                              tcgcgAGGATGTCATCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGc                                                                         <  3:217747/81‑1 (MQ=255)                                                                                                                                              tcgcgAGGATGTCATCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTggg                                                   <  4:88286/103‑1 (MQ=255)                                                                                                                                              tcgcgAGGATGTCATCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGAGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTggg                                                   >  3:88286/2‑104 (MQ=255)                                                                                                                                              gcgcgATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAATTTGCTGTTCCTTAACGATGAGCCAttt       >  4:113756/1‑148 (MQ=255)                                                                                                                                               cgcgATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAATTTGCTGTTCCTTAACGATGAGCCATTTCa     <  2:275425/149‑1 (MQ=255)                                                                                                                                                  gATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAATTTGCTGTTCCTTAACGATGAGCCATTTCAGGc  <  5:145978/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   CCCAACATTACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAATTTGCTGTTCCTTAACGATGAGCCATTTCAGGC  >  NC_002947/2826789‑2827081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑