Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,826,942 C→A 8.7% L119I (CTC→ATC)  PP_2479 → cytochrome c family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,826,9420CA 8.7% 157.8 / 2.6 52L119I (CTC→ATC) PP_2479cytochrome c family protein
Reads supporting (aligned to +/- strand):  ref base C (22/25);  new base A (3/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAATTTGCTGTTCCTTAACGATGAGCCATTTCAGGCGGACAGC  >  NC_002947/2826798‑2827088
                                                                                                                                                 |                                                                                                                                                  
aCCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTcg                                                                                                                                                 <  1:182816/148‑1 (MQ=255)
   ccGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGAt                                                                                                                                             >  2:42035/1‑149 (MQ=255)
       aCCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAg                                                                                                                                         <  6:72571/149‑1 (MQ=255)
        cccGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAgg                                                                                                                                        <  1:42035/149‑1 (MQ=255)
           gCAAACCTGCATCGGTTAATGGACCGATGACGAGTTCGTCAGTGAGCAGC‑AGAACTGCATTGGCATTGAAGGCAAGCACTACTACCCGTAGTTTACATATACATCATACAGCAAAATGTCGAGCGATGAAATCATCGCGATCAAGGGt                                                                                                                                      >  8:73279/1‑148 (MQ=255)
           gCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGt                                                                                                                                      >  2:85119/1‑148 (MQ=255)
              aaCCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTa                                                                                                                                    >  8:298788/1‑147 (MQ=255)
              aaCCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTa                                                                                                                                    <  7:298788/147‑1 (MQ=255)
               aCCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTAtct                                                                                                                                 <  1:202439/149‑1 (MQ=255)
                tcgtcATCGGTCCATGCACCGATGACGAGTTCGTCAGTGCGCTGCGAGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTAtct                                                                                                                                 >  1:234351/5‑149 (MQ=255)
                  ggCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGa                                                                                                                              <  2:99544/149‑1 (MQ=255)
                   gCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGa                                                                                                                              >  7:246353/1‑148 (MQ=255)
                     aTCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAg                                                                                                                           >  5:23811/1‑149 (MQ=255)
                                  ccGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGta                                                                                                              <  1:85119/149‑2 (MQ=255)
                                   cGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTc                                                                                                              >  6:220885/1‑148 (MQ=255)
                                    gATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGa                                                                                                            <  6:271606/149‑1 (MQ=255)
                                    gATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGa                                                                                                            <  8:246353/149‑1 (MQ=255)
                                      tGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGag                                                                                                           >  2:172126/1‑148 (MQ=255)
                                          gAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCg                                                                                                                               <  1:176424/124‑1 (MQ=255)
                                          gAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCg                                                                                                                               >  2:176424/1‑124 (MQ=255)
                                                          tGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACca                                                                                       >  5:210613/1‑148 (MQ=255)
                                                                  cGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCt                                                                               >  8:200841/1‑149 (MQ=255)
                                                                    gCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTc                                                                             >  4:154783/1‑149 (MQ=255)
                                                                     cATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTcc                                                                            <  2:242930/149‑1 (MQ=255)
                                                                      aTTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTccc                                                                           <  1:172126/149‑1 (MQ=255)
                                                                             gTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGa                                                                                                            <  4:206058/109‑1 (MQ=255)
                                                                             gTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGa                                                                                                            >  3:206058/1‑109 (MQ=255)
                                                                                aCGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCa                                                                  <  5:220885/148‑1 (MQ=255)
                                                                                             ctacCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATg                                                     <  2:303865/148‑1 (MQ=255)
                                                                                               acCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACcgcgc                                                                                               >  4:43551/1‑104 (MQ=255)
                                                                                               acCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACcgcgc                                                                                               <  3:43551/104‑1 (MQ=255)
                                                                                                 ccGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGtttt                                                >  1:318016/1‑149 (MQ=255)
                                                                                                 ccGGCGTTTCCCTATACCTCCAACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTGATCTCGACAGCCTGCGGCCGGTCGTGCAAGCACCGCGCGAAGACCACATCGGCGTCCCGTTCAATCAGCGCAGGGGCATGGttgt                                                >  8:205482/1‑147 (MQ=255)
                                                                                                  cGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTc                                               <  6:23811/149‑1 (MQ=255)
                                                                                                          cccTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATAAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAAttt                                       >  1:70946/1‑149 (MQ=255)
                                                                                                           ccTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGGTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAATTTg                                      >  1:251705/1‑149 (MQ=255)
                                                                                                            cTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTccc                                                                           <  8:92746/111‑1 (MQ=255)
                                                                                                            cTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTccc                                                                           >  7:92746/1‑111 (MQ=255)
                                                                                                                 cctcctACAGCAAAATGTCGCGCTATGACATCCTCGCGATCAATGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAATTTGCTGTTc                                <  7:73279/149‑1 (MQ=255)
                                                                                                                 cctcctACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAATTTGCTGtt                                 <  2:251705/148‑1 (MQ=255)
                                                                                                                          gCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTATGGAATTTGCTGTTCCTTAACGAt                       <  7:200841/149‑1 (MQ=255)
                                                                                                                           cAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCT‑GAATTTGCTGTTCCTTAACGAt                       >  3:93976/1‑147 (MQ=255)
                                                                                                                             aaaTGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAATTTGCTGTTCCTTAACGATGAg                    >  6:145978/1‑149 (MQ=255)
                                                                                                                                    tcgcgAGGATGTCATCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGc                                                                                <  3:217747/81‑1 (MQ=255)
                                                                                                                                    tcgcgAGGATGTCATCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGc                                                                                >  4:217747/2‑82 (MQ=255)
                                                                                                                                    tcgcgAGGATGTCATCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTggg                                                          <  4:88286/103‑1 (MQ=255)
                                                                                                                                    tcgcgAGGATGTCATCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGAGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTggg                                                          >  3:88286/2‑104 (MQ=255)
                                                                                                                                    gcgcgATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAATTTGCTGTTCCTTAACGATGAGCCAttt              >  4:113756/1‑148 (MQ=255)
                                                                                                                                     cgcgATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAATTTGCTGTTCCTTAACGATGAGCCATTTCa            <  2:275425/149‑1 (MQ=255)
                                                                                                                                        gATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAATTTGCTGTTCCTTAACGATGAGCCATTTCAGGc         <  5:145978/149‑1 (MQ=255)
                                                                                                                                            aCATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAATTTGCTGTTCCTTAACGATGAGCCATTTCAGGCGGa      <  5:99163/148‑1 (MQ=255)
                                                                                                                                               tCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAATTTGCTGTTCCTTAACGATGAGCCATTTCAGGCGGAcagc  <  2:318016/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
ACCCCGGACCCGCAAACCGGCATCGGTGCATGGACCGATGACGAGTTCGTCAGTGCGCTGC‑AGACCGGCATTGGCCGTGACGGCAAGCACTACTACCCGGCGTTTCCCTATACCTCCTACAGCAAAATGTCGCGCGATGACATCCTCGCGATCAAGGGTTATCTCGACAGCCTGCAACCGGTCGAGCAAGCACCGCGCGAAAACCACATCGGCTTCCCGTTCAATCAGCGCTGGGGCATGGTTTTCTGGAATTTGCTGTTCCTTAACGATGAGCCATTTCAGGCGGACAGC  >  NC_002947/2826798‑2827088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑